pycom17g19060

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002641
Physical Location & Seq
Forward (+)
16652045 .. 16654097
2053 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 825 bp
ATGTACAAAGCAGCCTCCCGACTCAGGTCCCTCAAGGGCCGGGGAACTTTGGGGGCCACCCGGTTTGCAACAAGTGCAGCTGTATCGAAGCCATCATCTGGGGGTCTCTTTAGCTGGCTGACGGGGGAGAGCTCAAGTGCTCTTCCTCCGTTGGAAACCCAACTCCCAGGTGTTAACCTTCCTCCTCCAGTACCTGATTATGTTGAACCAAGCAAGACGCAGATTACAACTCTTTCTAATGGTGTCAAAATAGCTTCAGAAACTTCAACGAGTGCTGCAGCGTCCATTGGAATATATGTGGATTCTGGTTCCATATATGAGACACCAGTATCCAGTGGTGCCTCACACTTGCTAGAACGATTGGCTTTTAAGAGTACAACCAACCGGAGCCACTTACGCATTGTGAGAGAAGTGGAAGCAATCGGTGGTCACATAGGAGCCAGTGCCTCTAGGGAGCAAATGGGATATACTTTTGATGCTATCAAGACCTATGTTCCCCAGATGGTGGAACTACTTGTTGACTCTGTGAGGAACCCTGCCTTCTTGGAATGGGAAGTCAATGAAGAGCTCAATAAGGTGCGGGCAGAGATTGGAGAACTTTCTAAGAATCCGCAGGGCTTACTCTTGGAGGCGATTCATTCTGCTGGTTATTCTGGTGCATTGGCTAATCCTCTTTTGGCTTCTGAATCATCACTGAACAGATTGAATGGCAGCATCTTGGAGCAGTTCATTGCTGAAAATTATACTGCTCCTCGTATAGTTCTTGCAGCATCTGGGGTTGATCGATCAGGAGATTGTATCCATTGCAGAACCACTTCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006508 GO:0006605 GO:0006626 GO:0006627 GO:0006807 GO:0006810 GO:0006839 GO:0006886 GO:0006950 GO:0006970 GO:0006996 GO:0007005 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010467 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016485 GO:0016787 GO:0017076 GO:0019538 GO:0019866 GO:0019867 GO:0030554 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034982 GO:0035639 GO:0036094 GO:0042221 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045184 GO:0045275 GO:0046872 GO:0046907 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0070585 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072655 GO:0097159 GO:0097367 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C

Protein Analysis

275

Amino Acids

29.09

Weight (kDa)

6.23

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 84 - 227 5.6e-41 Insulinase (Peptidase family M16)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 338
AccB7I CCANNNNNTGG 2 cut(s) 98, 505
AciI CCGC 2 cut(s) 580, 611
AcuI CTGAAG 1 cut(s) 240
AfaI GTAC 3 cut(s) 5, 192, 376
AfiI CCNNNNNNNGG 3 cut(s) 24, 98, 505
AgsI TTSAA 3 cut(s) 206, 267, 706
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 5 cut(s) 80, 114, 132, 254, 568
AluI AGCT 5 cut(s) 80, 114, 132, 254, 568
Alw21I GWGCWC 3 cut(s) 134, 142, 570
Alw26I GTCTC 2 cut(s) 110, 314
AlwNI CAGNNNCTG 2 cut(s) 194, 773
AoxI GGCC 2 cut(s) 37, 54
ApeKI GCWGC 6 cut(s) 11, 77, 275, 278, 711, 767
Asp700I GAANNNNTTC 1 cut(s) 814
AspS9I GGNCC 3 cut(s) 27, 37, 54
AsuC2I CCSGG 2 cut(s) 41, 61
AvaII GGWCC 1 cut(s) 27
BaeI ACNNNNGTAYC 2 cut(s) 312, 345
BanI GGYRCC 1 cut(s) 338
BanII GRGCYC 2 cut(s) 134, 570
Bbv12I GWGCWC 3 cut(s) 134, 142, 570
BbvI GCAGC 6 cut(s) 23, 89, 262, 290, 723, 779
BccI CCATC 2 cut(s) 100, 496
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BciT130I CCWGG 1 cut(s) 168
BciVI GTATCC 2 cut(s) 340, 809
BcnI CCSGG 2 cut(s) 41, 61
BcoDI GTCTC 2 cut(s) 110, 314
BfaI CTAG 2 cut(s) 353, 450
BfmI CTRYAG 1 cut(s) 276
BfuI GTATCC 2 cut(s) 340, 809
BisI GCNGC 6 cut(s) 12, 78, 276, 279, 712, 768
BlsI GCNGC 6 cut(s) 13, 79, 277, 280, 713, 769
Bme1390I CCNGG 3 cut(s) 41, 61, 168
Bme18I GGWCC 1 cut(s) 27
BmgT120I GGNCC 3 cut(s) 27, 37, 54
BmiI GGNNCC 7 cut(s) 29, 55, 310, 340, 389, 439, 533
BmrFI CCNGG 3 cut(s) 41, 61, 168
BmsI GCATC 3 cut(s) 466, 723, 779
BoxI GACNNNNGTC 1 cut(s) 25
BpmI CTGGAG 1 cut(s) 171
BpuEI CTTGAG 2 cut(s) 17, 118
BpuMI CCSGG 2 cut(s) 41, 61
Bsa29I ATCGAT 1 cut(s) 784
BsaI GGTCTC 1 cut(s) 110
BsaJI CCNNGG 2 cut(s) 40, 166
BsaWI WCCGGW 1 cut(s) 384
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 98, 505
Bse1I ACTGG 4 cut(s) 188, 326, 333, 441
Bse3DI GCAATG 2 cut(s) 729, 802
BseBI CCWGG 1 cut(s) 168
BseCI ATCGAT 1 cut(s) 784
BseDI CCNNGG 2 cut(s) 40, 166
BseLI CCNNNNNNNGG 3 cut(s) 24, 98, 505
BseMI GCAATG 2 cut(s) 729, 802
BseMII CTCAG 1 cut(s) 37
BseNI ACTGG 4 cut(s) 188, 326, 333, 441
BseRI GAGGAG 2 cut(s) 174, 741
BseXI GCAGC 6 cut(s) 23, 89, 262, 290, 723, 779
BsgI GTGCAG 1 cut(s) 96
BshFI GGCC 2 cut(s) 39, 56
BshNI GGYRCC 1 cut(s) 338
BshVI ATCGAT 1 cut(s) 784
BsiHKAI GWGCWC 3 cut(s) 134, 142, 570
BsiSI CCGG 3 cut(s) 40, 61, 385
BslFI GGGAC 1 cut(s) 13
BslI CCNNNNNNNGG 3 cut(s) 24, 98, 505
BsmAI GTCTC 2 cut(s) 110, 314
BsmFI GGGAC 1 cut(s) 13
BsnI GGCC 2 cut(s) 39, 56
Bso31I GGTCTC 1 cut(s) 110
Bsp1286I GDGCHC 3 cut(s) 134, 142, 570
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 781, 785
BspACI CCGC 2 cut(s) 580, 611
BspANI GGCC 2 cut(s) 39, 56
BspCNI CTCAG 1 cut(s) 36
BspDI ATCGAT 1 cut(s) 784
BspLI GGNNCC 7 cut(s) 29, 55, 310, 340, 389, 439, 533
BspMAI CTGCAG 1 cut(s) 280
BspQI GCTCTTC 2 cut(s) 147, 558
BspT107I GGYRCC 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 110
BsrDI GCAATG 2 cut(s) 729, 802
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 4 cut(s) 188, 326, 333, 441
BssECI CCNNGG 2 cut(s) 40, 166
BssMI GATC 2 cut(s) 781, 785
Bst2UI CCWGG 1 cut(s) 168
Bst6I CTCTTC 2 cut(s) 147, 558
BstAPI GCANNNNNTGC 1 cut(s) 74
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 116, 582
BstDEI CTNAG 2 cut(s) 23, 603
BstKTI GATC 2 cut(s) 784, 788
BstMAI GTCTC 2 cut(s) 110, 314
BstMBI GATC 2 cut(s) 781, 785
BstMWI GCNNNNNNNGC 2 cut(s) 74, 396
BstNI CCWGG 1 cut(s) 168
BstPAI GACNNNNGTC 1 cut(s) 25
BstSCI CCNGG 3 cut(s) 39, 59, 166
BstSFI CTRYAG 1 cut(s) 276
BstV1I GCAGC 6 cut(s) 23, 89, 262, 290, 723, 779
Bsu15I ATCGAT 1 cut(s) 784
BsuI GTATCC 2 cut(s) 340, 809
BsuRI GGCC 2 cut(s) 39, 56
BsuTUI ATCGAT 1 cut(s) 784
BtsIMutI CAGTG 3 cut(s) 340, 448, 692
Cac8I GCNNGC 2 cut(s) 116, 582
CaiI CAGNNNCTG 2 cut(s) 194, 773
Cfr13I GGNCC 3 cut(s) 27, 37, 54
ClaI ATCGAT 1 cut(s) 784
CseI GACGC 2 cut(s) 226, 270
Csp6I GTAC 3 cut(s) 4, 191, 375
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviQI GTAC 3 cut(s) 4, 191, 375
DdeI CTNAG 2 cut(s) 23, 603
DpnI GATC 2 cut(s) 783, 787
DpnII GATC 2 cut(s) 781, 785
Eam1104I CTCTTC 2 cut(s) 147, 558
EarI CTCTTC 2 cut(s) 147, 558
Ecl136II GAGCTC 2 cut(s) 132, 568
Eco24I GRGCYC 2 cut(s) 134, 570
Eco31I GGTCTC 1 cut(s) 110
Eco47I GGWCC 1 cut(s) 27
Eco53kI GAGCTC 2 cut(s) 132, 568
Eco57I CTGAAG 1 cut(s) 240
EcoICRI GAGCTC 2 cut(s) 132, 568
EcoO109I RGGNCCY 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 166
EcoT38I GRGCYC 2 cut(s) 134, 570
FaqI GGGAC 1 cut(s) 13
FauI CCCGC 1 cut(s) 573
Fnu4HI GCNGC 6 cut(s) 12, 78, 276, 279, 712, 768
FriOI GRGCYC 2 cut(s) 134, 570
Fsp4HI GCNGC 6 cut(s) 12, 78, 276, 279, 712, 768
FspBI CTAG 2 cut(s) 353, 450
GluI GCNGC 6 cut(s) 12, 78, 276, 279, 712, 768
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 2 cut(s) 39, 56
HapII CCGG 3 cut(s) 40, 61, 385
HgaI GACGC 2 cut(s) 226, 270
HincII GTYRAC 2 cut(s) 175, 520
HindII GTYRAC 2 cut(s) 175, 520
HinfI GANTC 6 cut(s) 21, 302, 521, 607, 634, 686
HpaI GTTAAC 1 cut(s) 175
HpaII CCGG 3 cut(s) 40, 61, 385
Hpy166II GTNNAC 2 cut(s) 175, 520
Hpy188I TCNGA 3 cut(s) 259, 685, 824
Hpy188III TCNNGA 3 cut(s) 18, 484, 789
Hpy8I GTNNAC 2 cut(s) 175, 520
HpyAV CCTTC 2 cut(s) 188, 550
HpyCH4V TGCA 6 cut(s) 68, 77, 278, 659, 767, 807
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 396
HpyF3I CTNAG 2 cut(s) 23, 603
KspAI GTTAAC 1 cut(s) 175
Kzo9I GATC 2 cut(s) 781, 785
LguI GCTCTTC 2 cut(s) 147, 558
LmnI GCTCC 5 cut(s) 387, 437, 454, 721, 754
Lsp1109I GCAGC 6 cut(s) 23, 89, 262, 290, 723, 779
LweI GCATC 3 cut(s) 466, 723, 779
MaeI CTAG 2 cut(s) 353, 450
MaeIII GTNAC 1 cut(s) 428
MalI GATC 2 cut(s) 783, 787
MboI GATC 2 cut(s) 781, 785
MboII GAAGA 2 cut(s) 134, 575
MhlI GDGCHC 3 cut(s) 134, 142, 570
MluCI AATT 1 cut(s) 739
MlyI GAGTC 2 cut(s) 15, 515
MmeI TCCRAC 1 cut(s) 132
MroXI GAANNNNTTC 1 cut(s) 814
MseI TTAA 2 cut(s) 174, 369
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 3 cut(s) 40, 61, 385
MspR9I CCNGG 3 cut(s) 41, 61, 168
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 74, 396
NciI CCSGG 2 cut(s) 41, 61
NdeII GATC 2 cut(s) 781, 785
NlaIV GGNNCC 7 cut(s) 29, 55, 310, 340, 389, 439, 533
NmuCI GTSAC 1 cut(s) 428
PciSI GCTCTTC 2 cut(s) 147, 558
PdmI GAANNNNTTC 1 cut(s) 814
PfeI GAWTC 4 cut(s) 302, 607, 634, 686
PflMI CCANNNNNTGG 2 cut(s) 98, 505
PkrI GCNGC 6 cut(s) 13, 79, 277, 280, 713, 769
PleI GAGTC 2 cut(s) 15, 515
PpsI GAGTC 2 cut(s) 15, 515
PpuMI RGGWCCY 1 cut(s) 27
PshAI GACNNNNGTC 1 cut(s) 25
Psp124BI GAGCTC 2 cut(s) 134, 570
Psp5II RGGWCCY 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 7 cut(s) 29, 55, 310, 340, 389, 439, 533
PspPI GGNCC 3 cut(s) 27, 37, 54
PspPPI RGGWCCY 1 cut(s) 27
PstI CTGCAG 1 cut(s) 280
PstNI CAGNNNCTG 2 cut(s) 194, 773
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 3 cut(s) 5, 192, 376
RsaNI GTAC 3 cut(s) 4, 191, 375
SacI GAGCTC 2 cut(s) 134, 570
SapI GCTCTTC 2 cut(s) 147, 558
SaqAI TTAA 2 cut(s) 174, 369
SatI GCNGC 6 cut(s) 12, 78, 276, 279, 712, 768
Sau3AI GATC 2 cut(s) 781, 785
Sau96I GGNCC 3 cut(s) 27, 37, 54
SchI GAGTC 2 cut(s) 15, 515
ScrFI CCNGG 3 cut(s) 41, 61, 168
SduI GDGCHC 3 cut(s) 134, 142, 570
SfaNI GCATC 3 cut(s) 466, 723, 779
SfcI CTRYAG 1 cut(s) 276
SinI GGWCC 1 cut(s) 27
SmlI CTYRAG 2 cut(s) 32, 133
SmoI CTYRAG 2 cut(s) 32, 133
Sse9I AATT 1 cut(s) 739
SsiI CCGC 2 cut(s) 580, 611
SspMI CTAG 2 cut(s) 353, 450
SstI GAGCTC 2 cut(s) 134, 570
StyD4I CCNGG 3 cut(s) 39, 59, 166
TaqI TCGA 2 cut(s) 86, 784
TasI AATT 1 cut(s) 739
TatI WGTACW 2 cut(s) 3, 374
TfiI GAWTC 4 cut(s) 302, 607, 634, 686
Tru1I TTAA 2 cut(s) 174, 369
Tru9I TTAA 2 cut(s) 174, 369
TscAI CASTG 3 cut(s) 340, 448, 699
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 6 cut(s) 11, 77, 275, 278, 711, 767
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 3 cut(s) 576, 626, 718
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 3 cut(s) 340, 448, 699
Van91I CCANNNNNTGG 2 cut(s) 98, 505
VpaK11BI GGWCC 1 cut(s) 27
XmnI GAANNNNTTC 1 cut(s) 814
XspI CTAG 2 cut(s) 353, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.