pycom17g19790

Cytochrome c-type biogenesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007193
Physical Location & Seq
Forward (+)
17802869 .. 17804968
2100 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGGGTAGCGAAGAGGACCCGGTGAAGAAGTCTCAGGTGGTGGATGCCCGGGCTCGGAACATTAGTCATAATGTTCGATGCACTGAGTGTGGGAGTCAGTCCATTGAAGATTCACAAGCGGATATTGCCATTTTGCTAAGAAAGTTAATTCGGGATGAAATTCATGCTGGGAAGAGCGATAAAGAGATATATAAGAAGCTCGAAGAGGATTTTGGGGAGACAGTACTTTATGCCCCAAAATTTGATCTGCAGACTGCAGGCTTGTGGTTATCACCGCTTGTCGTTGCTGGTGCTGCTGCAGGAATATGGGCATATAACAGGCACAGGCAAAAGACTAATGTCCACATCATGGCCTTGAACCTAGTTAGAGGTGTTCCATTGACCCCGAATGAGAAGCAAACCATGTTAGACCTCCTCACGCCTCCGCCTTCACGAGGAACTCCTTCCTCATTGTGGAGGAGGTGGAGAGAACAGTGA

Protein Analysis

159

Amino Acids

17.79

Weight (kDa)

8.58

Isoelectric Point (pI)

74.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CcmH PF03918 5 - 138 1.4e-29 Cytochrome C biogenesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 349
AciI CCGC 3 cut(s) 119, 275, 425
AcsI RAATTY 2 cut(s) 159, 239
AdeI CACNNNGTG 1 cut(s) 87
AfaI GTAC 1 cut(s) 225
AfiI CCNNNNNNNGG 4 cut(s) 54, 349, 434, 453
AgsI TTSAA 2 cut(s) 107, 358
AjuI GAANNNNNNNTTGG 2 cut(s) 195, 227
AluBI AGCT 1 cut(s) 199
AluI AGCT 1 cut(s) 199
Alw26I GTCTC 2 cut(s) 36, 212
Ama87I CYCGRG 1 cut(s) 48
AoxI GGCC 1 cut(s) 351
ApeKI GCWGC 2 cut(s) 293, 296
ApoI RAATTY 2 cut(s) 159, 239
Asp700I GAANNNNTTC 1 cut(s) 442
AspS9I GGNCC 1 cut(s) 16
AsuC2I CCSGG 3 cut(s) 20, 49, 50
AsuHPI GGTGA 2 cut(s) 34, 264
AvaI CYCGRG 1 cut(s) 48
AvaII GGWCC 1 cut(s) 16
BanII GRGCYC 1 cut(s) 55
BauI CACGAG 1 cut(s) 432
BbvI GCAGC 2 cut(s) 280, 283
BcnI CCSGG 3 cut(s) 20, 49, 50
BcoDI GTCTC 2 cut(s) 36, 212
BfaI CTAG 1 cut(s) 362
BfmI CTRYAG 3 cut(s) 248, 255, 297
BisI GCNGC 2 cut(s) 294, 297
BlsI GCNGC 2 cut(s) 295, 298
BmcAI AGTACT 1 cut(s) 225
Bme1390I CCNGG 3 cut(s) 20, 49, 50
Bme18I GGWCC 1 cut(s) 16
BmeT110I CYCGRG 1 cut(s) 48
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 3 cut(s) 20, 49, 50
BmsI GCATC 2 cut(s) 34, 68
BoxI GACNNNNGTC 1 cut(s) 338
BpuMI CCSGG 3 cut(s) 20, 49, 50
BsaJI CCNNGG 1 cut(s) 48
Bsc4I CCNNNNNNNGG 4 cut(s) 54, 349, 434, 453
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 2 cut(s) 49, 160
BseLI CCNNNNNNNGG 4 cut(s) 54, 349, 434, 453
BseMII CTCAG 2 cut(s) 47, 75
BseRI GAGGAG 2 cut(s) 404, 472
BseXI GCAGC 2 cut(s) 280, 283
BseYI CCCAGC 1 cut(s) 167
BshFI GGCC 1 cut(s) 353
BsiHKCI CYCGRG 1 cut(s) 48
BsiSI CCGG 2 cut(s) 20, 49
BslI CCNNNNNNNGG 4 cut(s) 54, 349, 434, 453
BsmAI GTCTC 2 cut(s) 36, 212
BsnI GGCC 1 cut(s) 353
BsoBI CYCGRG 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 55
Bsp143I GATC 1 cut(s) 244
BspACI CCGC 3 cut(s) 119, 275, 425
BspANI GGCC 1 cut(s) 353
BspCNI CTCAG 2 cut(s) 46, 76
BspLI GGNNCC 1 cut(s) 18
BspMAI CTGCAG 3 cut(s) 252, 259, 301
BspQI GCTCTTC 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 1 cut(s) 244
BssSI CACGAG 1 cut(s) 432
Bst2BI CACGAG 1 cut(s) 432
Bst4CI ACNGT 2 cut(s) 223, 474
Bst6I CTCTTC 3 cut(s) 6, 167, 198
BstC8I GCNNGC 1 cut(s) 259
BstDEI CTNAG 3 cut(s) 33, 84, 137
BstENI CCTNNNNNAGG 1 cut(s) 432
BstF5I GGATG 2 cut(s) 49, 160
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 2 cut(s) 36, 212
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 2 cut(s) 125, 293
BstPAI GACNNNNGTC 1 cut(s) 338
BstSCI CCNGG 3 cut(s) 18, 47, 48
BstSFI CTRYAG 3 cut(s) 248, 255, 297
BstV1I GCAGC 2 cut(s) 280, 283
BsuRI GGCC 1 cut(s) 353
BtsCI GGATG 2 cut(s) 49, 160
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 1 cut(s) 259
Cfr13I GGNCC 1 cut(s) 16
Cfr9I CCCGGG 1 cut(s) 48
Csp6I GTAC 1 cut(s) 224
CviAII CATG 3 cut(s) 164, 349, 403
CviJI RGCY 4 cut(s) 53, 199, 261, 353
CviKI_1 RGCY 4 cut(s) 53, 199, 261, 353
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 3 cut(s) 33, 84, 137
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
DraIII CACNNNGTG 1 cut(s) 87
Eam1104I CTCTTC 3 cut(s) 6, 167, 198
EarI CTCTTC 3 cut(s) 6, 167, 198
EciI GGCGGA 1 cut(s) 414
Eco24I GRGCYC 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 16
Eco88I CYCGRG 1 cut(s) 48
EcoNI CCTNNNNNAGG 1 cut(s) 432
EcoO109I RGGNCCY 1 cut(s) 16
EcoT38I GRGCYC 1 cut(s) 55
FaeI CATG 3 cut(s) 167, 352, 406
FatI CATG 3 cut(s) 163, 348, 402
Fnu4HI GCNGC 2 cut(s) 294, 297
FokI GGATG 2 cut(s) 56, 167
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 2 cut(s) 294, 297
FspBI CTAG 1 cut(s) 362
GluI GCNGC 2 cut(s) 294, 297
GsaI CCCAGC 1 cut(s) 171
HaeIII GGCC 1 cut(s) 353
HapII CCGG 2 cut(s) 20, 49
Hin1II CATG 3 cut(s) 167, 352, 406
HinfI GANTC 2 cut(s) 94, 110
HpaII CCGG 2 cut(s) 20, 49
HphI GGTGA 2 cut(s) 34, 264
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 1 cut(s) 57
Hpy188III TCNNGA 2 cut(s) 152, 432
Hpy8I GTNNAC 1 cut(s) 343
HpyAV CCTTC 2 cut(s) 438, 453
HpyCH4III ACNGT 2 cut(s) 223, 474
HpyCH4V TGCA 4 cut(s) 81, 250, 257, 299
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 293
HpyF3I CTNAG 3 cut(s) 33, 84, 137
Hsp92II CATG 3 cut(s) 167, 352, 406
Kzo9I GATC 1 cut(s) 244
LguI GCTCTTC 1 cut(s) 167
LpnPI CCDG 9 cut(s) 20, 33, 62, 153, 243, 273, 285, 304, 310
Lsp1109I GCAGC 2 cut(s) 280, 283
LweI GCATC 2 cut(s) 34, 68
MaeI CTAG 1 cut(s) 362
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 5 cut(s) 23, 37, 119, 184, 215
MhlI GDGCHC 1 cut(s) 55
MluCI AATT 3 cut(s) 147, 159, 239
MlyI GAGTC 1 cut(s) 103
MroXI GAANNNNTTC 1 cut(s) 442
MseI TTAA 1 cut(s) 146
MspI CCGG 2 cut(s) 20, 49
MspR9I CCNGG 3 cut(s) 20, 49, 50
MwoI GCNNNNNNNGC 2 cut(s) 125, 293
NciI CCSGG 3 cut(s) 20, 49, 50
NdeII GATC 1 cut(s) 244
NlaIII CATG 3 cut(s) 167, 352, 406
NlaIV GGNNCC 1 cut(s) 18
PciSI GCTCTTC 1 cut(s) 167
PdmI GAANNNNTTC 1 cut(s) 442
PfeI GAWTC 1 cut(s) 110
PflMI CCANNNNNTGG 1 cut(s) 349
PkrI GCNGC 2 cut(s) 295, 298
PleI GAGTC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 102
PpuMI RGGWCCY 1 cut(s) 16
PshAI GACNNNNGTC 1 cut(s) 338
Psp5II RGGWCCY 1 cut(s) 16
PspFI CCCAGC 1 cut(s) 167
PspN4I GGNNCC 1 cut(s) 18
PspPI GGNCC 1 cut(s) 16
PspPPI RGGWCCY 1 cut(s) 16
PstI CTGCAG 3 cut(s) 252, 259, 301
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 167
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 2 cut(s) 294, 297
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 1 cut(s) 16
ScaI AGTACT 1 cut(s) 225
SchI GAGTC 1 cut(s) 103
ScrFI CCNGG 3 cut(s) 20, 49, 50
SduI GDGCHC 1 cut(s) 55
SetI ASST 6 cut(s) 39, 201, 363, 373, 414, 464
SfaNI GCATC 2 cut(s) 34, 68
SfcI CTRYAG 3 cut(s) 248, 255, 297
SinI GGWCC 1 cut(s) 16
SmaI CCCGGG 1 cut(s) 50
SrfI GCCCGGGC 1 cut(s) 50
Sse9I AATT 3 cut(s) 147, 159, 239
SsiI CCGC 3 cut(s) 119, 275, 425
SspMI CTAG 1 cut(s) 362
StyD4I CCNGG 3 cut(s) 18, 47, 48
TaaI ACNGT 2 cut(s) 223, 474
TaqI TCGA 2 cut(s) 76, 201
TasI AATT 3 cut(s) 147, 159, 239
TatI WGTACW 1 cut(s) 223
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TscAI CASTG 1 cut(s) 88
TseI GCWGC 2 cut(s) 293, 296
TspDTI ATGAA 2 cut(s) 152, 171
TspMI CCCGGG 1 cut(s) 48
TspRI CASTG 1 cut(s) 88
Van91I CCANNNNNTGG 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 16
XagI CCTNNNNNAGG 1 cut(s) 432
XapI RAATTY 2 cut(s) 159, 239
XmaI CCCGGG 1 cut(s) 48
XmnI GAANNNNTTC 1 cut(s) 442
XspI CTAG 1 cut(s) 362
ZrmI AGTACT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.