Rh2DG424600

Cytochrome c-type biogenesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007193
Physical Location & Seq
Forward (+)
61537633 .. 61541842
4210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG424600.1

Sequence Viewer

Length: 657 bp
ATGAAAATTTTCAAGGGTCCAGATTTACTTGGTCAGGCTGGTAGCCGCACAGAAAATTTCTACCGTCACAGTTTCTTCATTTCCCTCTTTACATTTACTCCTTCATTCACTACGCCAAAGGTCGGAGAATTCCTTGATCACCAATTCGTACTCACGTTCCCGTTTGAGAGGTTGAGGGTGATGGAAAGCGAAGAGGACCCTGTGAAGAAGACTCAAGTGGTGGATGCCCGGGCTAGAAATATCAGTCATAATGTTAGGTGCACTGAGTGTGGGAGTCAGTCCATTGAGGATTCACAAGCTGATATCGCCATTCTTCTTAGAAAGTTAATTCGTGACGAGATTCATGCTGGGAAGAGTGACAAGGAGATCTACAAGAAGCTTGAGGAGGATTTTGGGGAGACGGTACTTTATGCCCCAAAATTTGATGTGCAGACTGCAGGCCTATGGCTATCACCGCTTGTGGTTGCCGGTGCTGCTGCAGGTATTTGGGCTTATAAGAAGCACAGACAAAGGACTAACGTCCACGTCATGGCTTTGAACCTTATTAGAGGTGTTCCACTGACCCCAAATGAGAAGCAAACCATGTTAGACCTCCTCACACCGCCATCTTCACGAGGAAATATACCTTCATCTTGGTGGAGGTGGAGAGAACAATGA

Protein Analysis

218

Amino Acids

24.94

Weight (kDa)

8.85

Isoelectric Point (pI)

53.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CcmH PF03918 60 - 198 1.6e-29 Cytochrome C biogenesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 495
Acc36I ACCTGC 1 cut(s) 470
AccB7I CCANNNNNTGG 1 cut(s) 529
AciI CCGC 3 cut(s) 46, 455, 602
AcsI RAATTY 4 cut(s) 6, 55, 128, 419
AdeI CACNNNGTG 1 cut(s) 267
AfaI GTAC 2 cut(s) 150, 405
AfiI CCNNNNNNNGG 2 cut(s) 122, 529
AgsI TTSAA 2 cut(s) 13, 538
AjiI CACGTC 1 cut(s) 526
AluBI AGCT 2 cut(s) 299, 379
AluI AGCT 2 cut(s) 299, 379
Alw21I GWGCWC 1 cut(s) 263
Alw26I GTCTC 1 cut(s) 392
Alw44I GTGCAC 1 cut(s) 259
Ama87I CYCGRG 1 cut(s) 228
AoxI GGCC 1 cut(s) 439
ApaLI GTGCAC 1 cut(s) 259
ApeKI GCWGC 2 cut(s) 473, 476
ApoI RAATTY 4 cut(s) 6, 55, 128, 419
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 17, 196
AsuC2I CCSGG 2 cut(s) 229, 230
AsuHPI GGTGA 3 cut(s) 131, 190, 444
AvaI CYCGRG 1 cut(s) 228
AvaII GGWCC 2 cut(s) 17, 196
BaeGI GKGCMC 1 cut(s) 263
BauI CACGAG 1 cut(s) 612
BbsI GAAGAC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 263
BbvI GCAGC 2 cut(s) 460, 463
BccI CCATC 2 cut(s) 175, 613
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BclI TGATCA 1 cut(s) 136
BcnI CCSGG 2 cut(s) 229, 230
BcoDI GTCTC 1 cut(s) 392
BfaI CTAG 1 cut(s) 234
BfmI CTRYAG 2 cut(s) 435, 477
BfuAI ACCTGC 1 cut(s) 470
BglII AGATCT 1 cut(s) 366
BisI GCNGC 3 cut(s) 46, 474, 477
BlsI GCNGC 3 cut(s) 47, 475, 478
Bme1390I CCNGG 2 cut(s) 229, 230
Bme18I GGWCC 2 cut(s) 17, 196
BmeT110I CYCGRG 1 cut(s) 228
BmgBI CACGTC 1 cut(s) 526
BmgT120I GGNCC 2 cut(s) 17, 196
BmiI GGNNCC 2 cut(s) 18, 198
BmrFI CCNGG 2 cut(s) 229, 230
BmsI GCATC 1 cut(s) 214
BoxI GACNNNNGTC 1 cut(s) 518
BpiI GAAGAC 1 cut(s) 215
BpuEI CTTGAG 2 cut(s) 198, 401
BpuMI CCSGG 2 cut(s) 229, 230
BsaJI CCNNGG 1 cut(s) 228
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 529
Bse118I RCCGGY 1 cut(s) 467
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 2 cut(s) 122, 529
BseMII CTCAG 1 cut(s) 255
BseRI GAGGAG 2 cut(s) 398, 584
BseSI GKGCMC 1 cut(s) 263
BseXI GCAGC 2 cut(s) 460, 463
BseYI CCCAGC 1 cut(s) 347
BsgI GTGCAG 1 cut(s) 449
BshFI GGCC 1 cut(s) 441
BsiHKAI GWGCWC 1 cut(s) 263
BsiHKCI CYCGRG 1 cut(s) 228
BsiSI CCGG 2 cut(s) 229, 468
BslI CCNNNNNNNGG 2 cut(s) 122, 529
BsmAI GTCTC 1 cut(s) 392
BsmBI CGTCTC 1 cut(s) 392
BsnI GGCC 1 cut(s) 441
BsoBI CYCGRG 1 cut(s) 228
Bsp1286I GDGCHC 1 cut(s) 263
Bsp143I GATC 2 cut(s) 136, 366
BspACI CCGC 3 cut(s) 46, 455, 602
BspANI GGCC 1 cut(s) 441
BspCNI CTCAG 1 cut(s) 256
BspLI GGNNCC 2 cut(s) 18, 198
BspMAI CTGCAG 2 cut(s) 439, 481
BspMI ACCTGC 1 cut(s) 470
BsrFI RCCGGY 1 cut(s) 467
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 2 cut(s) 136, 366
BssSI CACGAG 1 cut(s) 612
Bst2BI CACGAG 1 cut(s) 612
Bst4CI ACNGT 3 cut(s) 65, 71, 403
Bst6I CTCTTC 2 cut(s) 186, 347
BstC8I GCNNGC 1 cut(s) 439
BstDEI CTNAG 2 cut(s) 264, 317
BstF5I GGATG 1 cut(s) 229
BstKTI GATC 2 cut(s) 139, 369
BstMAI GTCTC 1 cut(s) 392
BstMBI GATC 2 cut(s) 136, 366
BstMWI GCNNNNNNNGC 3 cut(s) 305, 454, 473
BstPAI GACNNNNGTC 1 cut(s) 518
BstSCI CCNGG 2 cut(s) 227, 228
BstSFI CTRYAG 2 cut(s) 435, 477
BstSLI GKGCMC 1 cut(s) 263
BstV1I GCAGC 2 cut(s) 460, 463
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 366
BstYI RGATCY 1 cut(s) 366
BsuRI GGCC 1 cut(s) 441
BtrI CACGTC 1 cut(s) 526
BtsCI GGATG 1 cut(s) 229
BtsIMutI CAGTG 2 cut(s) 261, 557
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 1 cut(s) 439
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 2 cut(s) 17, 196
Cfr9I CCCGGG 1 cut(s) 228
Csp6I GTAC 2 cut(s) 149, 404
CviAII CATG 3 cut(s) 344, 529, 583
CviJI RGCY 9 cut(s) 38, 45, 233, 299, 379, 441, 448, 491, 533
CviKI_1 RGCY 9 cut(s) 38, 45, 233, 299, 379, 441, 448, 491, 533
CviQI GTAC 2 cut(s) 149, 404
DdeI CTNAG 2 cut(s) 264, 317
DpnI GATC 2 cut(s) 138, 368
DpnII GATC 2 cut(s) 136, 366
DraIII CACNNNGTG 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 186, 347
EarI CTCTTC 2 cut(s) 186, 347
Eco147I AGGCCT 1 cut(s) 441
Eco32I GATATC 1 cut(s) 304
Eco47I GGWCC 2 cut(s) 17, 196
Eco88I CYCGRG 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 196
EcoRI GAATTC 1 cut(s) 128
EcoRV GATATC 1 cut(s) 304
Esp3I CGTCTC 1 cut(s) 392
FaeI CATG 3 cut(s) 347, 532, 586
FaiI YATR 8 cut(s) 249, 345, 411, 445, 495, 530, 584, 623
FatI CATG 3 cut(s) 343, 528, 582
FbaI TGATCA 1 cut(s) 136
Fnu4HI GCNGC 3 cut(s) 46, 474, 477
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 3 cut(s) 46, 474, 477
FspBI CTAG 1 cut(s) 234
GluI GCNGC 3 cut(s) 46, 474, 477
GsaI CCCAGC 1 cut(s) 351
HaeIII GGCC 1 cut(s) 441
HapII CCGG 2 cut(s) 229, 468
Hin1II CATG 3 cut(s) 347, 532, 586
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 4 cut(s) 211, 274, 290, 340
HpaII CCGG 2 cut(s) 229, 468
HphI GGTGA 3 cut(s) 131, 190, 444
Hpy166II GTNNAC 2 cut(s) 261, 523
Hpy188I TCNGA 1 cut(s) 125
Hpy188III TCNNGA 3 cut(s) 20, 332, 612
Hpy8I GTNNAC 2 cut(s) 261, 523
HpyAV CCTTC 2 cut(s) 111, 636
HpyCH4III ACNGT 3 cut(s) 65, 71, 403
HpyCH4IV ACGT 3 cut(s) 155, 519, 525
HpyCH4V TGCA 4 cut(s) 261, 430, 437, 479
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 454, 473
HpyF3I CTNAG 2 cut(s) 264, 317
HpySE526I ACGT 3 cut(s) 155, 519, 525
Hsp92II CATG 3 cut(s) 347, 532, 586
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 2 cut(s) 136, 366
LpnPI CCDG 9 cut(s) 20, 24, 33, 213, 242, 333, 423, 465, 481
Lsp1109I GCAGC 2 cut(s) 460, 463
LweI GCATC 1 cut(s) 214
MaeI CTAG 1 cut(s) 234
MaeII ACGT 3 cut(s) 155, 519, 525
MaeIII GTNAC 3 cut(s) 65, 332, 356
MalI GATC 2 cut(s) 138, 368
MboI GATC 2 cut(s) 136, 366
MboII GAAGA 7 cut(s) 67, 203, 217, 220, 305, 364, 600
MflI RGATCY 1 cut(s) 366
MhlI GDGCHC 1 cut(s) 263
MluCI AATT 6 cut(s) 6, 55, 128, 143, 327, 419
MlyI GAGTC 2 cut(s) 205, 283
MmeI TCCRAC 1 cut(s) 103
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 1 cut(s) 326
MslI CAYNNNNRTG 1 cut(s) 634
MspI CCGG 2 cut(s) 229, 468
MspR9I CCNGG 2 cut(s) 229, 230
MwoI GCNNNNNNNGC 3 cut(s) 305, 454, 473
NciI CCSGG 2 cut(s) 229, 230
NdeII GATC 2 cut(s) 136, 366
NlaIII CATG 3 cut(s) 347, 532, 586
NlaIV GGNNCC 2 cut(s) 18, 198
NmuCI GTSAC 3 cut(s) 65, 332, 356
PceI AGGCCT 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 290, 340
PflMI CCANNNNNTGG 1 cut(s) 529
PkrI GCNGC 3 cut(s) 47, 475, 478
PleI GAGTC 2 cut(s) 205, 282
PpsI GAGTC 2 cut(s) 205, 282
PpuMI RGGWCCY 1 cut(s) 196
PshAI GACNNNNGTC 1 cut(s) 518
PsiI TTATAA 1 cut(s) 495
Psp5II RGGWCCY 1 cut(s) 196
PspFI CCCAGC 1 cut(s) 347
PspN4I GGNNCC 2 cut(s) 18, 198
PspPI GGNCC 2 cut(s) 17, 196
PspPPI RGGWCCY 1 cut(s) 196
PstI CTGCAG 2 cut(s) 439, 481
PsuI RGATCY 1 cut(s) 366
RsaI GTAC 2 cut(s) 150, 405
RsaNI GTAC 2 cut(s) 149, 404
RseI CAYNNNNRTG 1 cut(s) 634
SaqAI TTAA 1 cut(s) 326
SatI GCNGC 3 cut(s) 46, 474, 477
Sau3AI GATC 2 cut(s) 136, 366
Sau96I GGNCC 2 cut(s) 17, 196
SchI GAGTC 2 cut(s) 205, 283
ScrFI CCNGG 2 cut(s) 229, 230
SduI GDGCHC 1 cut(s) 263
SfaNI GCATC 1 cut(s) 214
SfcI CTRYAG 2 cut(s) 435, 477
SinI GGWCC 2 cut(s) 17, 196
SmaI CCCGGG 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 634
SmlI CTYRAG 2 cut(s) 213, 380
SmoI CTYRAG 2 cut(s) 213, 380
SrfI GCCCGGGC 1 cut(s) 230
Sse9I AATT 6 cut(s) 6, 55, 128, 143, 327, 419
SseBI AGGCCT 1 cut(s) 441
SsiI CCGC 3 cut(s) 46, 455, 602
SspMI CTAG 1 cut(s) 234
StuI AGGCCT 1 cut(s) 441
StyD4I CCNGG 2 cut(s) 227, 228
TaaI ACNGT 3 cut(s) 65, 71, 403
TaiI ACGT 3 cut(s) 158, 522, 528
TasI AATT 6 cut(s) 6, 55, 128, 143, 327, 419
TauI GCSGC 1 cut(s) 48
TfiI GAWTC 2 cut(s) 290, 340
Tru1I TTAA 1 cut(s) 326
Tru9I TTAA 1 cut(s) 326
TscAI CASTG 2 cut(s) 268, 564
TseFI GTSAC 3 cut(s) 65, 332, 356
TseI GCWGC 2 cut(s) 473, 476
Tsp45I GTSAC 3 cut(s) 65, 332, 356
TspDTI ATGAA 5 cut(s) 17, 67, 93, 332, 618
TspMI CCCGGG 1 cut(s) 228
TspRI CASTG 2 cut(s) 268, 564
Van91I CCANNNNNTGG 1 cut(s) 529
VneI GTGCAC 1 cut(s) 259
VpaK11BI GGWCC 2 cut(s) 17, 196
XapI RAATTY 4 cut(s) 6, 55, 128, 419
XmaI CCCGGG 1 cut(s) 228
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.