pycom17g20960

DNA replication complex GINS protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002339
Physical Location & Seq
Reverse (-)
19385791 .. 19388051
2261 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 459 bp
ATGTATGGGACAAATGCCATCCAACTGGTCAAAGAAATTGCAAATGGTGAAAAGGGGCAGCTCACGGCTTTCAGTAGTAATCTGTTCAAGGATGTAATTGCAGAATGTAGTCAACATTATGAAGATCTTCAAAAGTCGTTAAGGAAAATGCAGGAAGCAGGAATAGATTTCCAAACTCAAAATGAGGACCACTATGGTTTCCTTATCCACCACCTTTCTTTACTTCGCAATAAACGCTGCTTAATGACTTACATGCACAGCCGAGCAGAAATGTTACGGAATCTCATATGGAAGGTGGGCTATGAAATTCCAGAAGAAATTGAAGAGAAGCTTAGTCACACAGAGAAAGAGTACTTTAAGAAACATTCAGAAGCTTTAAGATCATACATGTCGAGAGTGGAACTCCATTTGGATATGGGTGCCCCCGAAGGACCCCTACATCAAGGTGAGAGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sld5

Protein Analysis

153

Amino Acids

17.71

Weight (kDa)

5.98

Isoelectric Point (pI)

68.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sld5 PF05916 60 - 133 9.8e-14 GINS complex protein helical bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 419
AcsI RAATTY 1 cut(s) 306
AfaI GTAC 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 387
AgsI TTSAA 3 cut(s) 88, 131, 323
AluBI AGCT 3 cut(s) 61, 331, 374
AluI AGCT 3 cut(s) 61, 331, 374
ApeKI GCWGC 2 cut(s) 58, 237
ApoI RAATTY 1 cut(s) 306
Asp700I GAANNNNTTC 1 cut(s) 126
AspS9I GGNCC 2 cut(s) 187, 431
AsuHPI GGTGA 2 cut(s) 59, 458
AvaII GGWCC 2 cut(s) 187, 431
BaeGI GKGCMC 1 cut(s) 424
BanI GGYRCC 1 cut(s) 419
BarI GAAGNNNNNNTAC 2 cut(s) 420, 452
BbvI GCAGC 2 cut(s) 70, 224
BccI CCATC 1 cut(s) 26
BceAI ACGGC 1 cut(s) 81
BglII AGATCT 1 cut(s) 124
BisI GCNGC 2 cut(s) 59, 238
BlsI GCNGC 2 cut(s) 60, 239
BmcAI AGTACT 1 cut(s) 353
Bme18I GGWCC 2 cut(s) 187, 431
BmgT120I GGNCC 2 cut(s) 187, 431
BmiI GGNNCC 2 cut(s) 421, 433
BplI GAGNNNNNCTC 2 cut(s) 387, 419
Bse1I ACTGG 1 cut(s) 30
BseGI GGATG 2 cut(s) 18, 97
BseNI ACTGG 1 cut(s) 30
BseSI GKGCMC 1 cut(s) 424
BseXI GCAGC 2 cut(s) 70, 224
BshNI GGYRCC 1 cut(s) 419
BslFI GGGAC 1 cut(s) 22
BsmFI GGGAC 1 cut(s) 22
Bsp1286I GDGCHC 1 cut(s) 424
Bsp143I GATC 2 cut(s) 124, 380
BspLI GGNNCC 2 cut(s) 421, 433
BspT107I GGYRCC 1 cut(s) 419
BsrI ACTGG 1 cut(s) 30
BssMI GATC 2 cut(s) 124, 380
Bst6I CTCTTC 1 cut(s) 318
BstDEI CTNAG 1 cut(s) 332
BstF5I GGATG 2 cut(s) 18, 97
BstKTI GATC 2 cut(s) 127, 383
BstMBI GATC 2 cut(s) 124, 380
BstMWI GCNNNNNNNGC 1 cut(s) 234
BstNSI RCATGY 2 cut(s) 256, 391
BstSLI GKGCMC 1 cut(s) 424
BstV1I GCAGC 2 cut(s) 70, 224
BstX2I RGATCY 1 cut(s) 124
BstXI CCANNNNNNTGG 1 cut(s) 25
BstYI RGATCY 1 cut(s) 124
BtsCI GGATG 2 cut(s) 18, 97
Cfr13I GGNCC 2 cut(s) 187, 431
Csp6I GTAC 1 cut(s) 352
CviAII CATG 2 cut(s) 253, 388
CviJI RGCY 6 cut(s) 61, 68, 261, 300, 331, 374
CviKI_1 RGCY 6 cut(s) 61, 68, 261, 300, 331, 374
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 1 cut(s) 332
DpnI GATC 2 cut(s) 126, 382
DpnII GATC 2 cut(s) 124, 380
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Eco47I GGWCC 2 cut(s) 187, 431
EcoO109I RGGNCCY 1 cut(s) 431
FaeI CATG 2 cut(s) 256, 391
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FaqI GGGAC 1 cut(s) 22
FatI CATG 2 cut(s) 252, 387
FauNDI CATATG 1 cut(s) 287
Fnu4HI GCNGC 2 cut(s) 59, 238
FokI GGATG 2 cut(s) 5, 104
Fsp4HI GCNGC 2 cut(s) 59, 238
GluI GCNGC 2 cut(s) 59, 238
Hin1II CATG 2 cut(s) 256, 391
HincII GTYRAC 1 cut(s) 113
HindII GTYRAC 1 cut(s) 113
HindIII AAGCTT 2 cut(s) 329, 372
HinfI GANTC 2 cut(s) 280, 451
HphI GGTGA 2 cut(s) 59, 458
Hpy166II GTNNAC 1 cut(s) 113
Hpy188I TCNGA 1 cut(s) 370
Hpy188III TCNNGA 2 cut(s) 311, 393
Hpy8I GTNNAC 1 cut(s) 113
HpyAV CCTTC 2 cut(s) 286, 422
HpyCH4V TGCA 4 cut(s) 41, 101, 151, 256
HpyF10VI GCNNNNNNNGC 1 cut(s) 234
HpyF3I CTNAG 1 cut(s) 332
Hsp92II CATG 2 cut(s) 256, 391
Kzo9I GATC 2 cut(s) 124, 380
LpnPI CCDG 4 cut(s) 11, 137, 144, 324
Lsp1109I GCAGC 2 cut(s) 70, 224
MaeIII GTNAC 2 cut(s) 273, 335
MalI GATC 2 cut(s) 126, 382
MboI GATC 2 cut(s) 124, 380
MboII GAAGA 4 cut(s) 119, 134, 326, 335
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 424
MluCI AATT 4 cut(s) 36, 96, 306, 318
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 1 cut(s) 178
MroXI GAANNNNTTC 1 cut(s) 126
MseI TTAA 4 cut(s) 140, 242, 357, 377
MslI CAYNNNNRTG 1 cut(s) 444
MwoI GCNNNNNNNGC 1 cut(s) 234
NdeI CATATG 1 cut(s) 287
NdeII GATC 2 cut(s) 124, 380
NlaIII CATG 2 cut(s) 256, 391
NlaIV GGNNCC 2 cut(s) 421, 433
NmeAIII GCCGAG 1 cut(s) 287
NmuCI GTSAC 1 cut(s) 335
NspI RCATGY 2 cut(s) 256, 391
PciI ACATGT 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 2 cut(s) 60, 239
PpuMI RGGWCCY 1 cut(s) 431
PscI ACATGT 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 431
PspN4I GGNNCC 2 cut(s) 421, 433
PspPI GGNCC 2 cut(s) 187, 431
PspPPI RGGWCCY 1 cut(s) 431
PsuI RGATCY 1 cut(s) 124
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 444
SaqAI TTAA 4 cut(s) 140, 242, 357, 377
SatI GCNGC 2 cut(s) 59, 238
Sau3AI GATC 2 cut(s) 124, 380
Sau96I GGNCC 2 cut(s) 187, 431
ScaI AGTACT 1 cut(s) 353
SduI GDGCHC 1 cut(s) 424
SetI ASST 6 cut(s) 63, 216, 297, 333, 376, 448
SinI GGWCC 2 cut(s) 187, 431
SmiMI CAYNNNNRTG 1 cut(s) 444
Sse9I AATT 4 cut(s) 36, 96, 306, 318
TaqI TCGA 1 cut(s) 392
TasI AATT 4 cut(s) 36, 96, 306, 318
TatI WGTACW 1 cut(s) 351
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 4 cut(s) 140, 242, 357, 377
Tru9I TTAA 4 cut(s) 140, 242, 357, 377
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 2 cut(s) 58, 237
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 2 cut(s) 135, 318
TspGWI ACGGA 1 cut(s) 292
VpaK11BI GGWCC 2 cut(s) 187, 431
XapI RAATTY 1 cut(s) 306
XceI RCATGY 2 cut(s) 256, 391
XmnI GAANNNNTTC 1 cut(s) 126
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.