pycom17g24330

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006585
Physical Location & Seq
Forward (+)
22656423 .. 22659772
3350 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1362 bp
ATGGCCTCTTCCGCTTCCGGAGAAGCCCTCAAACCCTCCGTCTCCAACAAACTCAAGCAGAAAGAGGGTACTTTGGACAGTGATGCTGGGAACTCACCTAGTGTAGTTGGATCAGAAGATGACGTTGATTTCCTAAAGAGTTCAACATCAGGATCAACCAATTCAAATGTTGTCAAAACTTCCAATTGTTTTTCATCTAATGATGATGATATGAGCATGGACGATGAAGACGACAACGACGACGACGATGATAATGACTATGATTTCGATGACAACGATGGTTTTCTGTATGATGTTGGTGATGATTTGCAATCTCAGTTTGATAATGTGGACTTGCCTCCCGGTGTAGAAGCAACTCTTCCCTGGTTGAATGATCCTTCACCGAGTGAGAAACCATCCACTAAGACGCTAGCTATTTCAGATCTACCACCATGTAGTTCAACTGTTCATTGTGAAGCAAGTTCAACTGTTCCTTCTGAGGCAAGTTCTACTAAGGAACCGGCAGACAGCAATGACGACAGTATCATGCAAACGTCTCAACAGTTTAAGCAATTTGATATGGTGGAAGATTTCTCGGATCACCACTTTAGCCGCGTGGGATATTCAGATGGGCAGCCACCCAAGGCTTGGGCTAAGCAAATTCAGGACGAGTGGAAGATTTTGGAGGACTTACCGGACACTATATTTGTCAGGGTTTATGAAGCTAGAATGGAACTTCTGAGGGCAGTAATTATAGGACCTCCGGGCACTCCCTACCATGATGGTCTTTTTGTCTTTGATTGTCTCTTCCCCACTGACTATCCTAAATCACCACCGATGGTATACTACTATTCAGGTGGTCTTCGATTGAATCCAAATTTGTACGAGTGTGGGAAAGTTTGCCTCAGTCTCTTAGGCACTTGGAGTGGCAAACAAAATGAAAATTGGGTCCCAGGGCAATCAACAATGCTACAAGTTTTAGTGTCTATACAAGCTCTTATTTTGAATGCAGAGCCCTTCTTCAATGAGCCTGGTCATGAGAGAACGTATGTTGGAGCAGAAGGGCGAAGGCATTCCCAAAAGTACAATGAGGATACCTTTATTCTATCTCTAAAGACAATGATGTATACACTAAGGAGGCCGCCTAAGTATTTTGAGGATTTTGTCTTGGGCCATTTTCGCGATCGTGCACACAATATTCTGGCAGCATGTAAAGCATATACTGAGGGCATTTTGGTAGGGTCTGATATCACATCCCAGAATGCAGAAAAAAGTGGCTCCTTACAATTCCGGGCAACTGTAGGTAAGATGATGAATACACTCGTTACACATTTCACCAGAAATGGATCAACCGACTGTGACAAGTTTCGAGTTGAAGCCTGA

Protein Analysis

454

Amino Acids

50.27

Weight (kDa)

4.45

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 215 - 340 1.3e-20 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 627
AccI GTMKAC 2 cut(s) 822, 1106
AccII CGCG 2 cut(s) 594, 1161
AccIII TCCGGA 1 cut(s) 17
AciI CCGC 3 cut(s) 12, 592, 1121
AclWI GGATC 5 cut(s) 118, 160, 368, 585, 1333
AcsI RAATTY 2 cut(s) 639, 856
AdeI CACNNNGTG 2 cut(s) 101, 386
AfaI GTAC 3 cut(s) 70, 863, 1064
AfiI CCNNNNNNNGG 1 cut(s) 627
AgsI TTSAA 9 cut(s) 144, 165, 370, 441, 465, 850, 985, 1003, 1355
AjnI CCWGG 3 cut(s) 362, 931, 1009
AluBI AGCT 3 cut(s) 413, 704, 974
AluI AGCT 3 cut(s) 413, 704, 974
Alw21I GWGCWC 1 cut(s) 1171
Alw26I GTCTC 4 cut(s) 46, 540, 788, 893
Alw44I GTGCAC 1 cut(s) 1167
AlwI GGATC 5 cut(s) 118, 160, 368, 585, 1333
Aor13HI TCCGGA 1 cut(s) 17
AoxI GGCC 3 cut(s) 3, 1118, 1150
ApaLI GTGCAC 1 cut(s) 1167
ApeKI GCWGC 2 cut(s) 613, 1184
ApoI RAATTY 2 cut(s) 639, 856
ArsI GACNNNNNNTTYG 6 cut(s) 24, 56, 248, 280, 668, 700
Asp700I GAANNNNTTC 1 cut(s) 1051
AspS9I GGNCC 3 cut(s) 737, 928, 1150
AsuC2I CCSGG 3 cut(s) 342, 744, 1271
AsuHPI GGTGA 6 cut(s) 87, 311, 372, 572, 801, 1306
AsuNHI GCTAGC 1 cut(s) 409
AvaII GGWCC 2 cut(s) 737, 928
BaeGI GKGCMC 2 cut(s) 749, 1171
BaeI ACNNNNGTAYC 2 cut(s) 505, 538
BanII GRGCYC 1 cut(s) 996
BbsI GAAGAC 2 cut(s) 234, 833
Bbv12I GWGCWC 1 cut(s) 1171
BbvI GCAGC 2 cut(s) 625, 1196
BccI CCATC 5 cut(s) 272, 403, 602, 755, 811
BciT130I CCWGG 3 cut(s) 364, 933, 1011
BciVI GTATCC 1 cut(s) 1066
BcnI CCSGG 3 cut(s) 342, 744, 1271
BcoDI GTCTC 4 cut(s) 46, 540, 788, 893
BfaI CTAG 3 cut(s) 99, 410, 705
BfmI CTRYAG 1 cut(s) 1278
BfuI GTATCC 1 cut(s) 1066
BglII AGATCT 1 cut(s) 421
BisI GCNGC 4 cut(s) 592, 614, 1121, 1185
BlpI GCTNAGC 1 cut(s) 633
BlsI GCNGC 4 cut(s) 593, 615, 1122, 1186
Bme1390I CCNGG 6 cut(s) 342, 364, 744, 933, 1011, 1271
Bme18I GGWCC 2 cut(s) 737, 928
BmgT120I GGNCC 3 cut(s) 737, 928, 1150
BmiI GGNNCC 4 cut(s) 498, 929, 930, 1258
BmrFI CCNGG 6 cut(s) 342, 364, 744, 933, 1011, 1271
BmsI GCATC 1 cut(s) 73
BmtI GCTAGC 1 cut(s) 413
BpiI GAAGAC 2 cut(s) 234, 833
BplI GAGNNNNNCTC 2 cut(s) 12, 44
Bpu1102I GCTNAGC 1 cut(s) 633
BpuEI CTTGAG 1 cut(s) 38
BpuMI CCSGG 3 cut(s) 342, 744, 1271
BsaJI CCNNGG 4 cut(s) 362, 621, 931, 932
BsaWI WCCGGW 2 cut(s) 17, 673
Bsc4I CCNNNNNNNGG 1 cut(s) 627
Bse118I RCCGGY 1 cut(s) 499
Bse3DI GCAATG 1 cut(s) 517
BseAI TCCGGA 1 cut(s) 17
BseBI CCWGG 3 cut(s) 364, 933, 1011
BseDI CCNNGG 4 cut(s) 362, 621, 931, 932
BseGI GGATG 2 cut(s) 395, 1232
BseLI CCNNNNNNNGG 1 cut(s) 627
BseMI GCAATG 1 cut(s) 517
BseMII CTCAG 5 cut(s) 329, 468, 710, 898, 1194
BseSI GKGCMC 2 cut(s) 749, 1171
BseXI GCAGC 2 cut(s) 625, 1196
BseYI CCCAGC 1 cut(s) 86
Bsh1236I CGCG 2 cut(s) 594, 1161
Bsh1285I CGRYCG 1 cut(s) 1165
BshFI GGCC 3 cut(s) 5, 1120, 1152
BsiEI CGRYCG 1 cut(s) 1165
BsiHKAI GWGCWC 1 cut(s) 1171
BsiSI CCGG 6 cut(s) 18, 342, 500, 674, 743, 1270
BslFI GGGAC 1 cut(s) 914
BslI CCNNNNNNNGG 1 cut(s) 627
BsmAI GTCTC 4 cut(s) 46, 540, 788, 893
BsmBI CGTCTC 2 cut(s) 46, 540
BsmFI GGGAC 1 cut(s) 914
BsmI GAATGC 3 cut(s) 991, 1051, 1246
BsnI GGCC 3 cut(s) 5, 1120, 1152
Bsp1286I GDGCHC 3 cut(s) 749, 996, 1171
Bsp13I TCCGGA 1 cut(s) 17
Bsp143I GATC 7 cut(s) 110, 152, 373, 421, 577, 1162, 1325
Bsp1720I GCTNAGC 1 cut(s) 633
Bsp68I TCGCGA 1 cut(s) 1161
BspACI CCGC 3 cut(s) 12, 592, 1121
BspANI GGCC 3 cut(s) 5, 1120, 1152
BspCNI CTCAG 5 cut(s) 328, 469, 711, 897, 1195
BspEI TCCGGA 1 cut(s) 17
BspFNI CGCG 2 cut(s) 594, 1161
BspHI TCATGA 1 cut(s) 1015
BspLI GGNNCC 4 cut(s) 498, 929, 930, 1258
BspOI GCTAGC 1 cut(s) 413
BspPI GGATC 5 cut(s) 118, 160, 368, 585, 1333
BsrDI GCAATG 1 cut(s) 517
BsrFI RCCGGY 1 cut(s) 499
BssAI RCCGGY 1 cut(s) 499
BssECI CCNNGG 4 cut(s) 362, 621, 931, 932
BssMI GATC 7 cut(s) 110, 152, 373, 421, 577, 1162, 1325
BssNAI GTATAC 2 cut(s) 823, 1107
BssT1I CCWWGG 1 cut(s) 621
Bst1107I GTATAC 2 cut(s) 823, 1107
Bst2UI CCWGG 3 cut(s) 364, 933, 1011
Bst4CI ACNGT 7 cut(s) 80, 445, 469, 521, 543, 1279, 1337
Bst6I CTCTTC 3 cut(s) 13, 363, 791
BstC8I GCNNGC 1 cut(s) 411
BstF5I GGATG 2 cut(s) 395, 1232
BstFNI CGCG 2 cut(s) 594, 1161
BstKTI GATC 7 cut(s) 113, 155, 376, 424, 580, 1165, 1328
BstMAI GTCTC 4 cut(s) 46, 540, 788, 893
BstMBI GATC 7 cut(s) 110, 152, 373, 421, 577, 1162, 1325
BstMCI CGRYCG 1 cut(s) 1165
BstMWI GCNNNNNNNGC 3 cut(s) 11, 1158, 1193
BstNI CCWGG 3 cut(s) 364, 933, 1011
BstNSI RCATGY 1 cut(s) 1191
BstSCI CCNGG 6 cut(s) 340, 362, 742, 931, 1009, 1269
BstSFI CTRYAG 1 cut(s) 1278
BstSLI GKGCMC 2 cut(s) 749, 1171
BstUI CGCG 2 cut(s) 594, 1161
BstV1I GCAGC 2 cut(s) 625, 1196
BstV2I GAAGAC 2 cut(s) 234, 833
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BstZ17I GTATAC 2 cut(s) 823, 1107
BsuI GTATCC 1 cut(s) 1066
BsuRI GGCC 3 cut(s) 5, 1120, 1152
BtsCI GGATG 2 cut(s) 395, 1232
BtsIMutI CAGTG 2 cut(s) 85, 792
BtuMI TCGCGA 1 cut(s) 1161
Cac8I GCNNGC 1 cut(s) 411
CciI TCATGA 1 cut(s) 1015
Cfr10I RCCGGY 1 cut(s) 499
Cfr13I GGNCC 3 cut(s) 737, 928, 1150
CseI GACGC 1 cut(s) 415
Csp6I GTAC 3 cut(s) 69, 862, 1063
CviAII CATG 6 cut(s) 217, 432, 526, 758, 1016, 1188
CviQI GTAC 3 cut(s) 69, 862, 1063
DpnI GATC 7 cut(s) 112, 154, 375, 423, 579, 1164, 1327
DpnII GATC 7 cut(s) 110, 152, 373, 421, 577, 1162, 1325
DraIII CACNNNGTG 2 cut(s) 101, 386
Eam1104I CTCTTC 3 cut(s) 13, 363, 791
EarI CTCTTC 3 cut(s) 13, 363, 791
Eco130I CCWWGG 1 cut(s) 621
Eco24I GRGCYC 1 cut(s) 996
Eco32I GATATC 1 cut(s) 1228
Eco47I GGWCC 2 cut(s) 737, 928
EcoO109I RGGNCCY 2 cut(s) 737, 928
EcoRII CCWGG 3 cut(s) 362, 931, 1009
EcoRV GATATC 1 cut(s) 1228
EcoT14I CCWWGG 1 cut(s) 621
EcoT38I GRGCYC 1 cut(s) 996
ErhI CCWWGG 1 cut(s) 621
Esp3I CGTCTC 2 cut(s) 46, 540
FaeI CATG 6 cut(s) 220, 435, 529, 761, 1019, 1191
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FaqI GGGAC 1 cut(s) 914
FatI CATG 6 cut(s) 216, 431, 525, 757, 1015, 1187
FblI GTMKAC 2 cut(s) 822, 1106
Fnu4HI GCNGC 4 cut(s) 592, 614, 1121, 1185
FokI GGATG 2 cut(s) 382, 1219
FriOI GRGCYC 1 cut(s) 996
Fsp4HI GCNGC 4 cut(s) 592, 614, 1121, 1185
FspBI CTAG 3 cut(s) 99, 410, 705
GluI GCNGC 4 cut(s) 592, 614, 1121, 1185
GsaI CCCAGC 1 cut(s) 90
HaeIII GGCC 3 cut(s) 5, 1120, 1152
HapII CCGG 6 cut(s) 18, 342, 500, 674, 743, 1270
HgaI GACGC 1 cut(s) 415
Hin1II CATG 6 cut(s) 220, 435, 529, 761, 1019, 1191
HinfI GANTC 1 cut(s) 850
HpaII CCGG 6 cut(s) 18, 342, 500, 674, 743, 1270
HphI GGTGA 6 cut(s) 87, 311, 372, 572, 801, 1306
Hpy166II GTNNAC 4 cut(s) 331, 823, 1107, 1169
Hpy188I TCNGA 7 cut(s) 115, 421, 478, 577, 607, 720, 1225
Hpy188III TCNNGA 5 cut(s) 18, 150, 644, 1016, 1160
Hpy8I GTNNAC 4 cut(s) 331, 823, 1107, 1169
Hpy99I CGWCG 3 cut(s) 242, 245, 248
HpyAV CCTTC 5 cut(s) 387, 483, 1006, 1034, 1041
HpyCH4III ACNGT 7 cut(s) 80, 445, 469, 521, 543, 1279, 1337
HpyCH4IV ACGT 3 cut(s) 123, 533, 1025
HpyCH4V TGCA 5 cut(s) 310, 529, 989, 1169, 1244
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 1158, 1193
HpySE526I ACGT 3 cut(s) 123, 533, 1025
Hsp92II CATG 6 cut(s) 220, 435, 529, 761, 1019, 1191
KflI GGGWCCC 1 cut(s) 928
Kpn2I TCCGGA 1 cut(s) 17
Kzo9I GATC 7 cut(s) 110, 152, 373, 421, 577, 1162, 1325
LmnI GCTCC 2 cut(s) 1034, 1262
Lsp1109I GCAGC 2 cut(s) 625, 1196
LweI GCATC 1 cut(s) 73
MaeI CTAG 3 cut(s) 99, 410, 705
MaeII ACGT 3 cut(s) 123, 533, 1025
MaeIII GTNAC 2 cut(s) 1303, 1337
MalI GATC 7 cut(s) 112, 154, 375, 423, 579, 1164, 1327
MboI GATC 7 cut(s) 110, 152, 373, 421, 577, 1162, 1325
MboII GAAGA 8 cut(s) 128, 239, 350, 578, 667, 778, 833, 991
MfeI CAATTG 1 cut(s) 184
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 3 cut(s) 749, 996, 1171
MluCI AATT 8 cut(s) 160, 184, 551, 639, 729, 856, 922, 1265
MmeI TCCRAC 3 cut(s) 69, 88, 1012
MroI TCCGGA 1 cut(s) 17
MroXI GAANNNNTTC 1 cut(s) 1051
MseI TTAA 1 cut(s) 546
MspI CCGG 6 cut(s) 18, 342, 500, 674, 743, 1270
MspR9I CCNGG 6 cut(s) 342, 364, 744, 933, 1011, 1271
MunI CAATTG 1 cut(s) 184
Mva1269I GAATGC 3 cut(s) 991, 1051, 1246
MvaI CCWGG 3 cut(s) 364, 933, 1011
MvnI CGCG 2 cut(s) 594, 1161
MwoI GCNNNNNNNGC 3 cut(s) 11, 1158, 1193
NciI CCSGG 3 cut(s) 342, 744, 1271
NdeII GATC 7 cut(s) 110, 152, 373, 421, 577, 1162, 1325
NheI GCTAGC 1 cut(s) 409
NlaIII CATG 6 cut(s) 220, 435, 529, 761, 1019, 1191
NlaIV GGNNCC 4 cut(s) 498, 929, 930, 1258
NmuCI GTSAC 1 cut(s) 1337
NruI TCGCGA 1 cut(s) 1161
NspI RCATGY 1 cut(s) 1191
PagI TCATGA 1 cut(s) 1015
PasI CCCWGGG 1 cut(s) 932
PcsI WCGNNNNNNNCGW 4 cut(s) 228, 237, 243, 273
PctI GAATGC 3 cut(s) 991, 1051, 1246
PdmI GAANNNNTTC 1 cut(s) 1051
PfeI GAWTC 1 cut(s) 850
PflMI CCANNNNNTGG 1 cut(s) 627
PkrI GCNGC 4 cut(s) 593, 615, 1122, 1186
Ple19I CGATCG 1 cut(s) 1165
PpuMI RGGWCCY 2 cut(s) 737, 928
Psp5II RGGWCCY 2 cut(s) 737, 928
Psp6I CCWGG 3 cut(s) 362, 931, 1009
PspFI CCCAGC 1 cut(s) 86
PspGI CCWGG 3 cut(s) 362, 931, 1009
PspN4I GGNNCC 4 cut(s) 498, 929, 930, 1258
PspPI GGNCC 3 cut(s) 737, 928, 1150
PspPPI RGGWCCY 2 cut(s) 737, 928
PsuI RGATCY 1 cut(s) 421
PvuI CGATCG 1 cut(s) 1165
RruI TCGCGA 1 cut(s) 1161
RsaI GTAC 3 cut(s) 70, 863, 1064
RsaNI GTAC 3 cut(s) 69, 862, 1063
SaqAI TTAA 1 cut(s) 546
SatI GCNGC 4 cut(s) 592, 614, 1121, 1185
Sau3AI GATC 7 cut(s) 110, 152, 373, 421, 577, 1162, 1325
Sau96I GGNCC 3 cut(s) 737, 928, 1150
ScrFI CCNGG 6 cut(s) 342, 364, 744, 933, 1011, 1271
SduI GDGCHC 3 cut(s) 749, 996, 1171
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 1278
SinI GGWCC 2 cut(s) 737, 928
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
Sse9I AATT 8 cut(s) 160, 184, 551, 639, 729, 856, 922, 1265
SsiI CCGC 3 cut(s) 12, 592, 1121
SspI AATATT 1 cut(s) 1177
SspMI CTAG 3 cut(s) 99, 410, 705
StyD4I CCNGG 6 cut(s) 340, 362, 742, 931, 1009, 1269
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 7 cut(s) 80, 445, 469, 521, 543, 1279, 1337
TaiI ACGT 3 cut(s) 126, 536, 1028
TaqI TCGA 3 cut(s) 267, 844, 1348
TasI AATT 8 cut(s) 160, 184, 551, 639, 729, 856, 922, 1265
TatI WGTACW 1 cut(s) 1062
TauI GCSGC 2 cut(s) 594, 1123
TfiI GAWTC 1 cut(s) 850
Tru1I TTAA 1 cut(s) 546
Tru9I TTAA 1 cut(s) 546
TscAI CASTG 2 cut(s) 85, 799
TseFI GTSAC 1 cut(s) 1337
TseI GCWGC 2 cut(s) 613, 1184
Tsp45I GTSAC 1 cut(s) 1337
TspDTI ATGAA 6 cut(s) 183, 240, 437, 714, 933, 1307
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 2 cut(s) 85, 799
Van91I CCANNNNNTGG 1 cut(s) 627
VneI GTGCAC 1 cut(s) 1167
VpaK11BI GGWCC 2 cut(s) 737, 928
XapI RAATTY 2 cut(s) 639, 856
XceI RCATGY 1 cut(s) 1191
XcmI CCANNNNNNNNNTGG 1 cut(s) 624
XmiI GTMKAC 2 cut(s) 822, 1106
XmnI GAANNNNTTC 1 cut(s) 1051
XspI CTAG 3 cut(s) 99, 410, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.