Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0002683

Physical Location & Seq

Genomic Coordinates Forward (+)

25084444 .. 25087373

Gene Structure 2930 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1881 bp

ATGAAGCCCCCGAAGCTGATTTCGTTACTGGTATGTTGGCTTGGTGTTTTCCTGACTGCAAATTCTGATCTGGCGTCCGACAGAGAGGCCTTGCTGGCGCTCAGGACCGCTGTGAGAGGCCGTATAATGGGGTGGAACGTGACGGAGACCAACCCCTGTTCGTGGCTCGGCGTCAAATGCGAGTCGGACCGGGTCACCGAGTTGAGGCTTCCGGGTCGGGCACTCGTCGGGAAGCTCCCTCCGGGCCTCGGGAACTTGACACAGCTCTTAAGCCTCTCTCTGCGCGTAAATGCGCTCTCCGGTCCACTCCCGAACGACCTTGTCAACTTAGTCAACCTTCAGAAACTCTACTTGCAAGGTAACTCGTTTTCCGGTCCAATTCCTGAGTTTTTGTTCAAATTGAAAAACCTTACGAGATTGAACCTTGATAAAAATAAATTCTCCGGTGAAATTTCTCCGGGGTTTAATAATTTGACGAGTTTGGTAAGTCTGCATTTGGAAGAAAATGAGCTTACCGGGTCAATTCCTGAGTTGGGTTTGTCGTCTCTGGGACAATTCAACGTTTCCTTTAACCAGTTGAACGGGTCGGTCCCGGCGAAGCTTTCAGGTTGGCCCAAAAACGCGTTTGAGGGGAATTTGCTCTGTGGGAAGCCGTTGAAGCCCTGTAATGAGACTGAGAGTGCTGGGAAGAAGAATAAGTTGGCTGGTGGGGCAATTGCAGGGATTGTGATTGGTAGTATTATTGGGTTGTTGGTGATTGTTGCCATTGTGATATTTTTGTGTAAAAGAAAGAAAAATGGCGAAAACGGTACAAACTTTGTCGTGACAGGGAAACCTCAGGAGGCTGAAACCGCCCGTGGAAGGGCGGTGATGGATAGGAGTTTGAGTACTGGTTCCTCATCTGCACCGAAGGGGAATGTAAAGAGTGCTGGTGGGAGTAAGAGTTTAGTGTTCTTCGGGGATGCGGTGAGGGTGTTTGATTTGGAGGATTTGTTGAGGGCATCCGCTGAGGTACTTGGAAAGGGGACTTTTGGGACTACGTACAAGGCCGCTTTGGATGAGGGGGTGTCAATGGCAGTGAAGAGGTTGAAGGAAGTGGCTGTATCAGAGAAGGAATTCAAGGAGAAGATTGAAGAGATTGGGAAAATGGATCATGTAAATTTGGTCCCACTCAGGGCTTACTACTATAGCAGGGATGAGAAGCTTCTTGTGTATGATTACATGCCTATGGGAAGCTTATCTGCTCTTTTGCACGGAGCCAGAGGCTCTGGAAGGACTCCATTGATTTGGGAAACAAGGTCTGGTATTGCTGTTGGAGCTGCTCGTGCAATTACATATCTACATTCTCAGGGTCCCACGACCTCCCATGGAAATATTAAATCTTCAAACATCCTTCTCACTAGATCCTATGAAGCCTGTGTCTCTGATTTTTGCCTTGCTCATCTTGCAAGCCCAACCTCTACTCCAAACCGCATTTCTGGTTATCGTGCCCCAGAACTCACAGATACTAGCAAAGTAACCCAAAAGGCAGATGTTTACAGCTTTGGCGTATTGCTTTTGGAGCTACTTACAGGAAAGCCTCCAACCCAAGCTATCATGAATGACGAAGGAGTAGACCTTCCAAGATGGGTTCACTCAGTGGTTCGAGAGGAGTGGACAGCTGAGGTGTTTGACTCCGAGCTTCTCAGGTACCAAAATGTTGAGGAGGATATGGTTCAGCTTTTACAGATTGCACTTGAATGTACTGTTACGTACCCTGATAGCCGCCCTTCAATGGGTGAGGTGACTAGCCGAATCCAGGAGCTTTACAGTTCCAGCTTACAGCACGGACATGATCCAAATCCTGATATATCTCATGATGGAGACGATGGACTTTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0008150 GO:0009505 GO:0009506 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016020 GO:0030054 GO:0030312 GO:0031090 GO:0031224 GO:0031225 GO:0031226 GO:0033993 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046658 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071944 GO:0097305 GO:0098588 GO:0098805 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

627

Amino Acids

67.83

Weight (kDa)

7.13

Isoelectric Point (pI)

30.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	25 - 60	4.7e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	83 - 193	1.4e-07	Leucine-rich repeat region
PK_Tyr_Ser-Thr	PF07714	337 - 598	2.3e-32	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	337 - 595	8.8e-32	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002683)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48480
fragaria_vesca	FvH4_2g12190 FvH4_2g12190 FvH4_5g17570
malus_domestica	MD02G1259100.v1.1 MD06G1231600.v1.1 MD09G1086600.v1.1 MD09G1271200.v1.1 MD14G1238300.v1.1 MD17G1269300.v1.1
prunus_persica	Prupe.3G017300_v2.0.a1 Prupe.5G235500_v2.0.a1
pyrus_communis	pycom06g20550 pycom09g18220 pycom14g19950 pycom17g26800
rosa_chinensis	RchiOBHm_Chr3g0491091 RchiOBHm_Chr7g0178081
rosa_laevigata	RLG00000005473 RLG00000022865
rosa_multiflora	Rmu_co8170494.1_g000001 Rmu_co8379533.1_g000001 Rmu_co8445967.1_g000001 Rmu_sc0000425.1_g000007 Rmu_sc0005038.1_g000014 Rmu_ssc0000386.1_g000061 Rmu_ssc0000386.1_g000063
rosa_roxburghii	Rroxscaffold_6G00393220
rosa_rugosa	Rorug03G0246000 Rorug06G0413300
rosa_samantha	Rh3AG296200 Rh3BG332200 Rh3CG329300 Rh3DG329900 Rh7AG012600 Rh7BG012500 Rh7CG012500 Rh7DG012800
rosa_wichuraiana	Rw3G026140 Rw7G000990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1689
AccB1I	GGYRCC	1 cut(s)	1689
AccI	GTMKAC	1 cut(s)	1614
AccII	CGCG	2 cut(s)	285, 623
AciI	CCGC	8 cut(s)	108, 852, 866, 965, 1005, 1050, 1471, 1765
AclI	AACGTT	1 cut(s)	561
AclWI	GGATC	3 cut(s)	1158, 1398, 1829
AcsI	RAATTY	6 cut(s)	61, 437, 450, 634, 1115, 1159
AcuI	CTGAAG	1 cut(s)	323
AcyI	GRCGYC	2 cut(s)	74, 171
AdeI	CACNNNGTG	1 cut(s)	1639
AfaI	GTAC	7 cut(s)	811, 889, 1014, 1043, 1691, 1744, 1754
AflII	CTTAAG	1 cut(s)	268
AflIII	ACRYGT	1 cut(s)	621
AjnI	CCWGG	1 cut(s)	1797
AjuI	GAANNNNNNNTTGG	4 cut(s)	683, 715, 1459, 1491
Alw26I	GTCTC	5 cut(s)	140, 549, 665, 1426, 1857
AlwI	GGATC	3 cut(s)	1158, 1398, 1829
Ama87I	CYCGRG	1 cut(s)	248
AoxI	GGCC	5 cut(s)	87, 118, 244, 611, 1047
ApeKI	GCWGC	1 cut(s)	1319
ApoI	RAATTY	6 cut(s)	61, 437, 450, 634, 1115, 1159
Asp700I	GAANNNNTTC	1 cut(s)	1115
Asp718I	GGTACC	1 cut(s)	1689
AspLEI	GCGC	3 cut(s)	100, 285, 295
AspS9I	GGNCC	9 cut(s)	105, 187, 244, 302, 374, 589, 612, 1165, 1352
AsuC2I	CCSGG	6 cut(s)	191, 213, 243, 459, 517, 593
AsuHPI	GGTGA	7 cut(s)	187, 458, 766, 880, 979, 1790, 1795
AvaI	CYCGRG	1 cut(s)	248
AvaII	GGWCC	7 cut(s)	105, 187, 302, 374, 589, 1165, 1352
AxyI	CCTNAGG	1 cut(s)	837
BaeGI	GKGCMC	2 cut(s)	223, 1492
BanI	GGYRCC	1 cut(s)	1689
BauI	CACGAG	1 cut(s)	1323
BbvCI	CCTCAGC	2 cut(s)	1008, 1662
BbvI	GCAGC	1 cut(s)	1306
BccI	CCATC	4 cut(s)	865, 1620, 1853, 1862
BceAI	ACGGC	2 cut(s)	105, 637
BciT130I	CCWGG	1 cut(s)	1799
BcnI	CCSGG	6 cut(s)	191, 213, 243, 459, 517, 593
BcoDI	GTCTC	5 cut(s)	140, 549, 665, 1426, 1857
BfaI	CTAG	3 cut(s)	1401, 1509, 1788
BfmI	CTRYAG	1 cut(s)	1186
BfoI	RGCGCY	1 cut(s)	101
BfrI	CTTAAG	1 cut(s)	268
BglI	GCCNNNNNGGC	1 cut(s)	95
BisI	GCNGC	3 cut(s)	1050, 1320, 1765
BlsI	GCNGC	3 cut(s)	1051, 1321, 1766
BmcAI	AGTACT	1 cut(s)	889
Bme1390I	CCNGG	7 cut(s)	191, 213, 243, 459, 517, 593, 1799
Bme18I	GGWCC	7 cut(s)	105, 187, 302, 374, 589, 1165, 1352
BmeT110I	CYCGRG	1 cut(s)	248
BmgT120I	GGNCC	9 cut(s)	105, 187, 244, 302, 374, 589, 612, 1165, 1352
BmiI	GGNNCC	7 cut(s)	591, 895, 1167, 1258, 1353, 1354, 1691
BmrFI	CCNGG	7 cut(s)	191, 213, 243, 459, 517, 593, 1799
BmsI	GCATC	2 cut(s)	952, 1010
Bpu10I	CCTNAGC	3 cut(s)	101, 1008, 1662
BpuMI	CCSGG	6 cut(s)	191, 213, 243, 459, 517, 593
BsaAI	YACGTR	2 cut(s)	1041, 1752
BsaBI	GATNNNNATC	1 cut(s)	1839
BsaHI	GRCGYC	2 cut(s)	74, 171
BsaI	GGTCTC	1 cut(s)	140
BsaJI	CCNNGG	4 cut(s)	247, 458, 856, 1366
BsaWI	WCCGGW	3 cut(s)	299, 371, 443
BsaXI	ACNNNNNCTCC	8 cut(s)	871, 901, 928, 958, 977, 1007, 1447, 1477
Bse1I	ACTGG	3 cut(s)	33, 574, 895
Bse21I	CCTNAGG	1 cut(s)	837
Bse8I	GATNNNNATC	1 cut(s)	1839
BseBI	CCWGG	1 cut(s)	1799
BseDI	CCNNGG	4 cut(s)	247, 458, 856, 1366
BseGI	GGATG	5 cut(s)	967, 1001, 1063, 1201, 1389
BseJI	GATNNNNATC	1 cut(s)	1839
BseNI	ACTGG	3 cut(s)	33, 574, 895
BseRI	GAGGAG	2 cut(s)	1664, 1718
BseSI	GKGCMC	2 cut(s)	223, 1492
BseXI	GCAGC	1 cut(s)	1306
BseYI	CCCAGC	1 cut(s)	683
BsgI	GTGCAG	1 cut(s)	888
Bsh1236I	CGCG	2 cut(s)	285, 623
BshFI	GGCC	5 cut(s)	89, 120, 246, 613, 1049
BshNI	GGYRCC	1 cut(s)	1689
BsiHKCI	CYCGRG	1 cut(s)	248
BsiSI	CCGG	9 cut(s)	190, 212, 242, 300, 372, 444, 458, 516, 593
BslFI	GGGAC	6 cut(s)	564, 575, 1039, 1048, 1151, 1338
BsmAI	GTCTC	5 cut(s)	140, 549, 665, 1426, 1857
BsmBI	CGTCTC	2 cut(s)	549, 1857
BsmFI	GGGAC	6 cut(s)	564, 575, 1039, 1048, 1151, 1338
BsnI	GGCC	5 cut(s)	89, 120, 246, 613, 1049
Bso31I	GGTCTC	1 cut(s)	140
BsoBI	CYCGRG	1 cut(s)	248
Bsp1286I	GDGCHC	2 cut(s)	223, 1492
Bsp143I	GATC	4 cut(s)	67, 1150, 1403, 1834
Bsp19I	CCATGG	1 cut(s)	1366
BspACI	CCGC	8 cut(s)	108, 852, 866, 965, 1005, 1050, 1471, 1765
BspANI	GGCC	5 cut(s)	89, 120, 246, 613, 1049
BspFNI	CGCG	2 cut(s)	285, 623
BspHI	TCATGA	2 cut(s)	1596, 1855
BspLI	GGNNCC	7 cut(s)	591, 895, 1167, 1258, 1353, 1354, 1691
BspPI	GGATC	3 cut(s)	1158, 1398, 1829
BspT107I	GGYRCC	1 cut(s)	1689
BspTI	CTTAAG	1 cut(s)	268
BspTNI	GGTCTC	1 cut(s)	140
BsrI	ACTGG	3 cut(s)	33, 574, 895
BssECI	CCNNGG	4 cut(s)	247, 458, 856, 1366
BssMI	GATC	4 cut(s)	67, 1150, 1403, 1834
BssNI	GRCGYC	2 cut(s)	74, 171
BssSI	CACGAG	1 cut(s)	1323
BssT1I	CCWWGG	1 cut(s)	1366
Bst2BI	CACGAG	1 cut(s)	1323
Bst2UI	CCWGG	1 cut(s)	1799
Bst4CI	ACNGT	3 cut(s)	809, 1747, 1811
Bst6I	CTCTTC	2 cut(s)	1076, 1128
BstACI	GRCGYC	2 cut(s)	74, 171
BstAFI	CTTAAG	1 cut(s)	268
BstBAI	YACGTR	2 cut(s)	1041, 1752
BstC8I	GCNNGC	2 cut(s)	96, 1450
BstDSI	CCRYGG	2 cut(s)	856, 1366
BstEII	GGTNACC	1 cut(s)	193
BstF5I	GGATG	5 cut(s)	967, 1001, 1063, 1201, 1389
BstFNI	CGCG	2 cut(s)	285, 623
BstH2I	RGCGCY	1 cut(s)	101
BstHHI	GCGC	3 cut(s)	100, 285, 295
BstKTI	GATC	4 cut(s)	70, 1153, 1406, 1837
BstMAI	GTCTC	5 cut(s)	140, 549, 665, 1426, 1857
BstMBI	GATC	4 cut(s)	67, 1150, 1403, 1834
BstNI	CCWGG	1 cut(s)	1799
BstNSI	RCATGY	1 cut(s)	1225
BstPI	GGTNACC	1 cut(s)	193
BstSCI	CCNGG	7 cut(s)	189, 211, 241, 457, 515, 591, 1797
BstSFI	CTRYAG	1 cut(s)	1186
BstSLI	GKGCMC	2 cut(s)	223, 1492
BstSNI	TACGTA	2 cut(s)	1041, 1752
BstUI	CGCG	2 cut(s)	285, 623
BstV1I	GCAGC	1 cut(s)	1306
BstX2I	RGATCY	1 cut(s)	1403
BstXI	CCANNNNNNTGG	1 cut(s)	1287
BstYI	RGATCY	1 cut(s)	1403
Bsu36I	CCTNAGG	1 cut(s)	837
BsuRI	GGCC	5 cut(s)	89, 120, 246, 613, 1049
BtgI	CCRYGG	2 cut(s)	856, 1366
BtsCI	GGATG	5 cut(s)	967, 1001, 1063, 1201, 1389
BtsI	GCAGTG	1 cut(s)	1083
BtsIMutI	CAGTG	2 cut(s)	1083, 1644
Cac8I	GCNNGC	2 cut(s)	96, 1450
CciI	TCATGA	2 cut(s)	1596, 1855
CfoI	GCGC	3 cut(s)	100, 285, 295
Cfr13I	GGNCC	9 cut(s)	105, 187, 244, 302, 374, 589, 612, 1165, 1352
CpoI	CGGWCCG	1 cut(s)	187
CseI	GACGC	2 cut(s)	63, 160
Csp6I	GTAC	7 cut(s)	810, 888, 1013, 1042, 1690, 1743, 1753
CspI	CGGWCCG	1 cut(s)	187
CviAII	CATG	6 cut(s)	1154, 1222, 1367, 1597, 1832, 1856
CviQI	GTAC	7 cut(s)	810, 888, 1013, 1042, 1690, 1743, 1753
DpnI	GATC	4 cut(s)	69, 1152, 1405, 1836
DpnII	GATC	4 cut(s)	67, 1150, 1403, 1834
DraIII	CACNNNGTG	1 cut(s)	1639
Eam1104I	CTCTTC	2 cut(s)	1076, 1128
EarI	CTCTTC	2 cut(s)	1076, 1128
Eco105I	TACGTA	2 cut(s)	1041, 1752
Eco130I	CCWWGG	1 cut(s)	1366
Eco147I	AGGCCT	1 cut(s)	89
Eco31I	GGTCTC	1 cut(s)	140
Eco47I	GGWCC	7 cut(s)	105, 187, 302, 374, 589, 1165, 1352
Eco57I	CTGAAG	1 cut(s)	323
Eco81I	CCTNAGG	1 cut(s)	837
Eco88I	CYCGRG	1 cut(s)	248
Eco91I	GGTNACC	1 cut(s)	193
EcoO109I	RGGNCCY	1 cut(s)	1352
EcoO65I	GGTNACC	1 cut(s)	193
EcoRI	GAATTC	1 cut(s)	1115
EcoRII	CCWGG	1 cut(s)	1797
EcoT14I	CCWWGG	1 cut(s)	1366
ErhI	CCWWGG	1 cut(s)	1366
Esp3I	CGTCTC	2 cut(s)	549, 1857
FaeI	CATG	6 cut(s)	1157, 1225, 1370, 1600, 1835, 1859
FaqI	GGGAC	6 cut(s)	564, 575, 1039, 1048, 1151, 1338
FatI	CATG	6 cut(s)	1153, 1221, 1366, 1596, 1831, 1855
FblI	GTMKAC	1 cut(s)	1614
Fnu4HI	GCNGC	3 cut(s)	1050, 1320, 1765
FokI	GGATG	5 cut(s)	974, 988, 1070, 1208, 1376
Fsp4HI	GCNGC	3 cut(s)	1050, 1320, 1765
FspBI	CTAG	3 cut(s)	1401, 1509, 1788
GlaI	GCGC	3 cut(s)	99, 284, 294
GluI	GCNGC	3 cut(s)	1050, 1320, 1765
GsaI	CCCAGC	1 cut(s)	687
HaeII	RGCGCY	1 cut(s)	101
HaeIII	GGCC	5 cut(s)	89, 120, 246, 613, 1049
HapII	CCGG	9 cut(s)	190, 212, 242, 300, 372, 444, 458, 516, 593
HgaI	GACGC	2 cut(s)	63, 160
HhaI	GCGC	3 cut(s)	100, 285, 295
Hin1I	GRCGYC	2 cut(s)	74, 171
Hin1II	CATG	6 cut(s)	1157, 1225, 1370, 1600, 1835, 1859
Hin6I	GCGC	3 cut(s)	98, 283, 293
HinP1I	GCGC	3 cut(s)	98, 283, 293
HincII	GTYRAC	2 cut(s)	325, 334
HindII	GTYRAC	2 cut(s)	325, 334
HindIII	AAGCTT	3 cut(s)	599, 1202, 1234
HinfI	GANTC	4 cut(s)	182, 1276, 1673, 1794
HpaII	CCGG	9 cut(s)	190, 212, 242, 300, 372, 444, 458, 516, 593
HphI	GGTGA	7 cut(s)	187, 458, 766, 880, 979, 1790, 1795
Hpy166II	GTNNAC	7 cut(s)	305, 325, 334, 1537, 1615, 1633, 1656
Hpy188I	TCNGA	7 cut(s)	67, 79, 187, 342, 1108, 1426, 1678
Hpy8I	GTNNAC	7 cut(s)	305, 325, 334, 1537, 1615, 1633, 1656
Hpy99I	CGWCG	1 cut(s)	230
HpyCH4III	ACNGT	3 cut(s)	809, 1747, 1811
HpyCH4IV	ACGT	4 cut(s)	138, 561, 1040, 1751
HpyCH4V	TGCA	9 cut(s)	59, 355, 493, 719, 905, 1252, 1328, 1448, 1733
HpySE526I	ACGT	4 cut(s)	138, 561, 1040, 1751
Hsp92I	GRCGYC	2 cut(s)	74, 171
Hsp92II	CATG	6 cut(s)	1157, 1225, 1370, 1600, 1835, 1859
HspAI	GCGC	3 cut(s)	98, 283, 293
KflI	GGGWCCC	1 cut(s)	1352
KpnI	GGTACC	1 cut(s)	1693
Kzo9I	GATC	4 cut(s)	67, 1150, 1403, 1834
LmnI	GCTCC	5 cut(s)	240, 1256, 1316, 1561, 1801
Lsp1109I	GCAGC	1 cut(s)	1306
LweI	GCATC	2 cut(s)	952, 1010
MaeI	CTAG	3 cut(s)	1401, 1509, 1788
MaeII	ACGT	4 cut(s)	138, 561, 1040, 1751
MaeIII	GTNAC	8 cut(s)	24, 139, 193, 359, 823, 1516, 1747, 1783
MalI	GATC	4 cut(s)	69, 1152, 1405, 1836
MboI	GATC	4 cut(s)	67, 1150, 1403, 1834
MboII	GAAGA	8 cut(s)	512, 700, 703, 946, 1093, 1138, 1145, 1374
MfeI	CAATTG	1 cut(s)	714
MflI	RGATCY	1 cut(s)	1403
MhlI	GDGCHC	2 cut(s)	223, 1492
MluI	ACGCGT	1 cut(s)	621
MlyI	GAGTC	3 cut(s)	191, 1270, 1667
MmeI	TCCRAC	4 cut(s)	102, 165, 1294, 1607
MroXI	GAANNNNTTC	1 cut(s)	1115
MseI	TTAA	4 cut(s)	269, 465, 570, 1377
MslI	CAYNNNNRTG	3 cut(s)	1226, 1738, 1830
MspA1I	CMGCKG	3 cut(s)	110, 1007, 1661
MspCI	CTTAAG	1 cut(s)	268
MspI	CCGG	9 cut(s)	190, 212, 242, 300, 372, 444, 458, 516, 593
MspR9I	CCNGG	7 cut(s)	191, 213, 243, 459, 517, 593, 1799
MunI	CAATTG	1 cut(s)	714
MvaI	CCWGG	1 cut(s)	1799
MvnI	CGCG	2 cut(s)	285, 623
NciI	CCSGG	6 cut(s)	191, 213, 243, 459, 517, 593
NcoI	CCATGG	1 cut(s)	1366
NdeII	GATC	4 cut(s)	67, 1150, 1403, 1834
NlaIII	CATG	6 cut(s)	1157, 1225, 1370, 1600, 1835, 1859
NlaIV	GGNNCC	7 cut(s)	591, 895, 1167, 1258, 1353, 1354, 1691
NmeAIII	GCCGAG	1 cut(s)	147
NmuCI	GTSAC	4 cut(s)	139, 193, 823, 1783
NspI	RCATGY	1 cut(s)	1225
PagI	TCATGA	2 cut(s)	1596, 1855
PceI	AGGCCT	1 cut(s)	89
PcsI	WCGNNNNNNNCGW	1 cut(s)	593
PdmI	GAANNNNTTC	1 cut(s)	1115
PfeI	GAWTC	1 cut(s)	1794
PflFI	GACNNNGTC	2 cut(s)	191, 320
PfoI	TCCNGGA	1 cut(s)	1797
PkrI	GCNGC	3 cut(s)	1051, 1321, 1766
PleI	GAGTC	3 cut(s)	190, 1270, 1667
PpsI	GAGTC	3 cut(s)	190, 1270, 1667
Ppu21I	YACGTR	2 cut(s)	1041, 1752
PpuMI	RGGWCCY	1 cut(s)	1352
Psp1406I	AACGTT	1 cut(s)	561
Psp5II	RGGWCCY	1 cut(s)	1352
Psp6I	CCWGG	1 cut(s)	1797
PspEI	GGTNACC	1 cut(s)	193
PspFI	CCCAGC	1 cut(s)	683
PspGI	CCWGG	1 cut(s)	1797
PspN4I	GGNNCC	7 cut(s)	591, 895, 1167, 1258, 1353, 1354, 1691
PspPI	GGNCC	9 cut(s)	105, 187, 244, 302, 374, 589, 612, 1165, 1352
PspPPI	RGGWCCY	1 cut(s)	1352
PsuI	RGATCY	1 cut(s)	1403
PsyI	GACNNNGTC	2 cut(s)	191, 320
PvuII	CAGCTG	1 cut(s)	1661
RsaI	GTAC	7 cut(s)	811, 889, 1014, 1043, 1691, 1744, 1754
RsaNI	GTAC	7 cut(s)	810, 888, 1013, 1042, 1690, 1743, 1753
RseI	CAYNNNNRTG	3 cut(s)	1226, 1738, 1830
Rsr2I	CGGWCCG	1 cut(s)	187
RsrII	CGGWCCG	1 cut(s)	187
SaqAI	TTAA	4 cut(s)	269, 465, 570, 1377
SatI	GCNGC	3 cut(s)	1050, 1320, 1765
Sau3AI	GATC	4 cut(s)	67, 1150, 1403, 1834
Sau96I	GGNCC	9 cut(s)	105, 187, 244, 302, 374, 589, 612, 1165, 1352
ScaI	AGTACT	1 cut(s)	889
SchI	GAGTC	3 cut(s)	191, 1270, 1667
ScrFI	CCNGG	7 cut(s)	191, 213, 243, 459, 517, 593, 1799
SduI	GDGCHC	2 cut(s)	223, 1492
SfaNI	GCATC	2 cut(s)	952, 1010
SfcI	CTRYAG	1 cut(s)	1186
SinI	GGWCC	7 cut(s)	105, 187, 302, 374, 589, 1165, 1352
SmiMI	CAYNNNNRTG	3 cut(s)	1226, 1738, 1830
SmlI	CTYRAG	1 cut(s)	268
SmoI	CTYRAG	1 cut(s)	268
SnaBI	TACGTA	2 cut(s)	1041, 1752
SseBI	AGGCCT	1 cut(s)	89
SsiI	CCGC	8 cut(s)	108, 852, 866, 965, 1005, 1050, 1471, 1765
SspI	AATATT	1 cut(s)	1375
SspMI	CTAG	3 cut(s)	1401, 1509, 1788
StuI	AGGCCT	1 cut(s)	89
StyD4I	CCNGG	7 cut(s)	189, 211, 241, 457, 515, 591, 1797
StyI	CCWWGG	1 cut(s)	1366
TaaI	ACNGT	3 cut(s)	809, 1747, 1811
TaiI	ACGT	4 cut(s)	141, 564, 1043, 1754
TaqI	TCGA	1 cut(s)	1645
TaqII	GACCGA	1 cut(s)	577
TatI	WGTACW	2 cut(s)	887, 1742
TauI	GCSGC	2 cut(s)	1052, 1767
TfiI	GAWTC	1 cut(s)	1794
Tru1I	TTAA	4 cut(s)	269, 465, 570, 1377
Tru9I	TTAA	4 cut(s)	269, 465, 570, 1377
TscAI	CASTG	2 cut(s)	1083, 1644
TseFI	GTSAC	4 cut(s)	139, 193, 823, 1783
TseI	GCWGC	1 cut(s)	1319
Tsp45I	GTSAC	4 cut(s)	139, 193, 823, 1783
TspDTI	ATGAA	3 cut(s)	17, 1425, 1613
TspGWI	ACGGA	3 cut(s)	158, 1269, 1842
TspRI	CASTG	2 cut(s)	1083, 1644
Tth111I	GACNNNGTC	2 cut(s)	191, 320
Vha464I	CTTAAG	1 cut(s)	268
VpaK11BI	GGWCC	7 cut(s)	105, 187, 302, 374, 589, 1165, 1352
XapI	RAATTY	6 cut(s)	61, 437, 450, 634, 1115, 1159
XceI	RCATGY	1 cut(s)	1225
XmiI	GTMKAC	1 cut(s)	1614
XmnI	GAANNNNTTC	1 cut(s)	1115
XspI	CTAG	3 cut(s)	1401, 1509, 1788
ZrmI	AGTACT	1 cut(s)	889

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.