pycom17g26860

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005732
Physical Location & Seq
Forward (+)
25164517 .. 25167274
2758 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
ATGTTGCAACCGAAACAAACAATAAGCACCCACAGTTTTTTTCATTTGAGCTCATATCATCTATTTATATTTATGGAGAAGGCACACTCCAAATCTACTCTTAAGTTTGTGAAGAGTGCTTCACAGTCTACAACAGGTTCATGGAGCTCGAATTCTGAAGGAGCGCCAAAAGATGAATTCAAGGACAAATCAAAGTCTTGTCAAAAGTCTCCAACAAGGGAAAATACCAAAGCTCAAGAAGTTAAACTTCACACTAAAGAAAGAGCTACCAAAAGAGCAATGTTCAACTACATGGTTGCAACCAAGCTGAGTATCTTGGAGCACCAGAGAAAACAAGAAGAGAGGTTGCAGAAGATGATAGAAGAGGAAGAGATTCGCTTACTGAGAAAGGAGATGGTTCCAAGGGCTCGATTGATGCCTTTTTTCGATAGACCTTTCATTCCACAAAGATCAAGCAGGCCCTTGACGATTCCTAGGGAACCAAGTTTCCGCAAGTACTTAAGCTGCAACCCTGGCAGTCAACTCTACAATTTCCAACCGACAACTCGAGCTACGAATCCCATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

190

Amino Acids

22.14

Weight (kDa)

10.14

Isoelectric Point (pI)

58.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 82 - 156 2.6e-23 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 128
AciI CCGC 1 cut(s) 490
AcsI RAATTY 2 cut(s) 151, 176
AcuI CTGAAG 1 cut(s) 177
AfaI GTAC 1 cut(s) 497
AflII CTTAAG 2 cut(s) 101, 499
AgsI TTSAA 2 cut(s) 181, 286
AjnI CCWGG 1 cut(s) 511
AloI GAACNNNNNNTCC 2 cut(s) 471, 503
AluBI AGCT 7 cut(s) 51, 147, 233, 266, 307, 504, 551
AluI AGCT 7 cut(s) 51, 147, 233, 266, 307, 504, 551
Alw21I GWGCWC 3 cut(s) 53, 149, 324
Alw26I GTCTC 1 cut(s) 213
Ama87I CYCGRG 1 cut(s) 546
AoxI GGCC 1 cut(s) 458
ApeKI GCWGC 1 cut(s) 504
ApoI RAATTY 2 cut(s) 151, 176
Asp700I GAANNNNTTC 1 cut(s) 372
AspA2I CCTAGG 1 cut(s) 473
AspLEI GCGC 1 cut(s) 166
AspS9I GGNCC 1 cut(s) 459
AvaI CYCGRG 1 cut(s) 546
AvrII CCTAGG 1 cut(s) 473
BanII GRGCYC 3 cut(s) 53, 149, 409
Bbv12I GWGCWC 3 cut(s) 53, 149, 324
BbvI GCAGC 1 cut(s) 491
BccI CCATC 2 cut(s) 388, 569
BciT130I CCWGG 1 cut(s) 513
BcoDI GTCTC 1 cut(s) 213
BfaI CTAG 1 cut(s) 474
BfoI RGCGCY 1 cut(s) 167
BfrI CTTAAG 2 cut(s) 101, 499
BisI GCNGC 1 cut(s) 505
BlnI CCTAGG 1 cut(s) 473
BlsI GCNGC 1 cut(s) 506
BmcAI AGTACT 1 cut(s) 497
Bme1390I CCNGG 1 cut(s) 513
BmeT110I CYCGRG 1 cut(s) 546
BmgT120I GGNCC 1 cut(s) 459
BmiI GGNNCC 2 cut(s) 399, 480
BmrFI CCNGG 1 cut(s) 513
BmsI GCATC 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 401, 473, 511
Bse3DI GCAATG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 3 cut(s) 401, 473, 511
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 2 cut(s) 299, 374
BseXI GCAGC 1 cut(s) 491
BshFI GGCC 1 cut(s) 460
BsiHKAI GWGCWC 3 cut(s) 53, 149, 324
BsiHKCI CYCGRG 1 cut(s) 546
BsmAI GTCTC 1 cut(s) 213
BsnI GGCC 1 cut(s) 460
BsoBI CYCGRG 1 cut(s) 546
Bsp1286I GDGCHC 4 cut(s) 53, 149, 324, 409
Bsp143I GATC 1 cut(s) 449
BspACI CCGC 1 cut(s) 490
BspANI GGCC 1 cut(s) 460
BspCNI CTCAG 2 cut(s) 300, 375
BspLI GGNNCC 2 cut(s) 399, 480
BspTI CTTAAG 2 cut(s) 101, 499
BsrDI GCAATG 1 cut(s) 285
BssECI CCNNGG 3 cut(s) 401, 473, 511
BssMI GATC 1 cut(s) 449
BssT1I CCWWGG 2 cut(s) 401, 473
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 2 cut(s) 35, 126
Bst6I CTCTTC 4 cut(s) 107, 333, 357, 363
BstAFI CTTAAG 2 cut(s) 101, 499
BstC8I GCNNGC 1 cut(s) 458
BstDEI CTNAG 2 cut(s) 308, 383
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 1 cut(s) 452
BstMAI GTCTC 1 cut(s) 213
BstMBI GATC 1 cut(s) 449
BstMWI GCNNNNNNNGC 1 cut(s) 513
BstNI CCWGG 1 cut(s) 513
BstSCI CCNGG 1 cut(s) 511
BstV1I GCAGC 1 cut(s) 491
BsuRI GGCC 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 458
CfoI GCGC 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 459
Csp6I GTAC 1 cut(s) 496
CviAII CATG 2 cut(s) 141, 292
CviJI RGCY 9 cut(s) 51, 147, 233, 266, 307, 407, 460, 504, 551
CviKI_1 RGCY 9 cut(s) 51, 147, 233, 266, 307, 407, 460, 504, 551
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 2 cut(s) 308, 383
DpnI GATC 1 cut(s) 451
DpnII GATC 1 cut(s) 449
Eam1104I CTCTTC 4 cut(s) 107, 333, 357, 363
EarI CTCTTC 4 cut(s) 107, 333, 357, 363
Ecl136II GAGCTC 2 cut(s) 51, 147
Eco130I CCWWGG 2 cut(s) 401, 473
Eco24I GRGCYC 3 cut(s) 53, 149, 409
Eco53kI GAGCTC 2 cut(s) 51, 147
Eco57I CTGAAG 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 546
EcoICRI GAGCTC 2 cut(s) 51, 147
EcoO109I RGGNCCY 1 cut(s) 459
EcoRI GAATTC 2 cut(s) 151, 176
EcoRII CCWGG 1 cut(s) 511
EcoT14I CCWWGG 2 cut(s) 401, 473
EcoT38I GRGCYC 3 cut(s) 53, 149, 409
ErhI CCWWGG 2 cut(s) 401, 473
FaeI CATG 2 cut(s) 144, 295
FaiI YATR 5 cut(s) 55, 68, 74, 142, 293
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FatI CATG 2 cut(s) 140, 291
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 1 cut(s) 505
FriOI GRGCYC 3 cut(s) 53, 149, 409
Fsp4HI GCNGC 1 cut(s) 505
FspBI CTAG 1 cut(s) 474
GlaI GCGC 1 cut(s) 165
GluI GCNGC 1 cut(s) 505
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 1 cut(s) 460
HhaI GCGC 1 cut(s) 166
Hin1II CATG 2 cut(s) 144, 295
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HincII GTYRAC 1 cut(s) 521
HindII GTYRAC 1 cut(s) 521
HinfI GANTC 3 cut(s) 373, 469, 556
Hpy166II GTNNAC 2 cut(s) 129, 521
Hpy188I TCNGA 1 cut(s) 157
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 2 cut(s) 129, 521
HpyAV CCTTC 2 cut(s) 73, 152
HpyCH4III ACNGT 2 cut(s) 35, 126
HpyCH4V TGCA 4 cut(s) 7, 299, 349, 507
HpyF10VI GCNNNNNNNGC 1 cut(s) 513
HpyF3I CTNAG 2 cut(s) 308, 383
Hsp92II CATG 2 cut(s) 144, 295
HspAI GCGC 1 cut(s) 164
Kzo9I GATC 1 cut(s) 449
LmnI GCTCC 3 cut(s) 144, 161, 319
LpnPI CCDG 5 cut(s) 120, 338, 442, 498, 525
Lsp1109I GCAGC 1 cut(s) 491
LweI GCATC 1 cut(s) 405
MaeI CTAG 1 cut(s) 474
MalI GATC 1 cut(s) 451
MboI GATC 1 cut(s) 449
MboII GAAGA 5 cut(s) 124, 350, 364, 374, 380
MhlI GDGCHC 4 cut(s) 53, 149, 324, 409
MluCI AATT 3 cut(s) 151, 176, 529
MmeI TCCRAC 2 cut(s) 236, 559
MnlI CCTC 2 cut(s) 336, 358
MroXI GAANNNNTTC 1 cut(s) 372
MseI TTAA 3 cut(s) 102, 243, 500
MspCI CTTAAG 2 cut(s) 101, 499
MspR9I CCNGG 1 cut(s) 513
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 1 cut(s) 513
NdeII GATC 1 cut(s) 449
NlaIII CATG 2 cut(s) 144, 295
NlaIV GGNNCC 2 cut(s) 399, 480
PaeR7I CTCGAG 1 cut(s) 546
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 3 cut(s) 373, 469, 556
PkrI GCNGC 1 cut(s) 506
Psp124BI GAGCTC 2 cut(s) 53, 149
Psp6I CCWGG 1 cut(s) 511
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 2 cut(s) 399, 480
PspPI GGNCC 1 cut(s) 459
PspXI VCTCGAGB 1 cut(s) 546
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
SacI GAGCTC 2 cut(s) 53, 149
SaqAI TTAA 3 cut(s) 102, 243, 500
SatI GCNGC 1 cut(s) 505
Sau3AI GATC 1 cut(s) 449
Sau96I GGNCC 1 cut(s) 459
ScaI AGTACT 1 cut(s) 497
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 4 cut(s) 53, 149, 324, 409
SfaNI GCATC 1 cut(s) 405
Sfr274I CTCGAG 1 cut(s) 546
SlaI CTCGAG 1 cut(s) 546
SmlI CTYRAG 4 cut(s) 101, 234, 499, 546
SmoI CTYRAG 4 cut(s) 101, 234, 499, 546
Sse9I AATT 3 cut(s) 151, 176, 529
SsiI CCGC 1 cut(s) 490
SspMI CTAG 1 cut(s) 474
SstI GAGCTC 2 cut(s) 53, 149
StyD4I CCNGG 1 cut(s) 511
StyI CCWWGG 2 cut(s) 401, 473
TaaI ACNGT 2 cut(s) 35, 126
TaqI TCGA 4 cut(s) 149, 409, 426, 547
TasI AATT 3 cut(s) 151, 176, 529
TatI WGTACW 1 cut(s) 495
TfiI GAWTC 3 cut(s) 373, 469, 556
Tru1I TTAA 3 cut(s) 102, 243, 500
Tru9I TTAA 3 cut(s) 102, 243, 500
TseI GCWGC 1 cut(s) 504
TspDTI ATGAA 4 cut(s) 32, 129, 189, 427
Vha464I CTTAAG 2 cut(s) 101, 499
XapI RAATTY 2 cut(s) 151, 176
XhoI CTCGAG 1 cut(s) 546
XmaJI CCTAGG 1 cut(s) 473
XmiI GTMKAC 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 372
XspI CTAG 1 cut(s) 474
ZrmI AGTACT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.