pycom520g00290

Protein of unknown function (DUF3727)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0005107
Physical Location & Seq
Forward (+)
250651 .. 258779
8129 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1176 bp
ATGCATAAAAAACAAAAGAAAAAATATTTGAGGATTGAATTATTACTTAAAATGATGATTAACATGTTTATTTCTTATTATTACACTTACAGTCTGCCGCCAAAGCGTGAAAGAAGAAACTTCAACTTGGGTCTTCTCCTCGCTCACGCTTGCCAGACGAACATGGCATTCTCCTCACTCTCACTGTCCACAACTCCATCAACCACCACCTGCAGTCTCCATTCCAAGCGACCCCACCACCTCCCCTTGTTCGCTTTCCACTCCAACCAACATCTATTCCCACTCTCAATCTCGCTCCCATCCCCTCCTTCATCGACCCCTGCCAGACTCCGTATCTCTCTCTGCGCCGCCGCAAAGTCACAGACTGGCCCCGTCAAGAAGCGCTCACCTCCCACCAACAACAACAAGAAGAAAAAGCGCAAGGGCGGCAGTGGCGGTGATAATTCCGAGGATCCGAGCTTCAGTGATGTTGAAATTGTGGATAGTTGTGGTGGTGGGGTTTCAAATTCGAGGTCGTTGGATTTCCATCCAACACCGCTGCCAAATCCGCCGGCTGGGTTTGTGGTGGATGATCATGGGAAGGTGCTCATGGCTTCTTCCAAGCGAATTGCTTCCATTGTCGATCCTACCAATAATTTCCCTCTAGAGTGTGTTATAAGGAGAGTATTCAGAAGCTCGCGAGGGGATGAATGTATGCTGCTTTGCCCAGTTGACACGCCCGTTCAAATATTAAGGAGCACAAACATAGATGGCTGGTCAGCTGTCAGCGATGAAGAAGTTGAAGCTATTCTTCCCTCTGCTGCTTATGCCCTTGCTAAGATACACATGCATCTAGTACATAGTGGATTCTGCTATACAGCACGGGGAGGGTTTTGTTACTCCGAAGATGACATATTTGATTTCCGTACAGATGATGGTCAAGATGTAGATGGCCTGCCAACCGAGGGTGTTGAAATTACATGTTTCCATTTGGATGGTTCACATTACATGATTTATACGCCGTCTGACCCACTTCTCTTTGTTGTTGTAAAGGATAATGCTGGTTTGCTGCAAATTGCTGACGATGACATGTTGGAAGATCCTGCTATCATTAGTGCCATAGATGAGGAGACTGAGTTTAACGCCTTGGTGGAGGAAGAAGCGGCTCTTCTTGATTCATTGCTAGGAAAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3727

Protein Analysis

392

Amino Acids

43.11

Weight (kDa)

5.87

Isoelectric Point (pI)

62.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3727 PF12527 275 - 369 1.4e-19 Protein of unknown function (DUF3727)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 656
AarI CACCTGC 1 cut(s) 218
Acc36I ACCTGC 1 cut(s) 218
AccII CGCG 1 cut(s) 679
AciI CCGC 8 cut(s) 98, 348, 351, 426, 435, 536, 548, 1142
AclWI GGATC 4 cut(s) 446, 459, 617, 1073
AcsI RAATTY 1 cut(s) 505
AcuI CTGAAG 1 cut(s) 445
AfaI GTAC 2 cut(s) 837, 907
AfeI AGCGCT 1 cut(s) 383
AfiI CCNNNNNNNGG 2 cut(s) 554, 944
AflIII ACRYGT 3 cut(s) 63, 959, 1068
AgsI TTSAA 7 cut(s) 38, 124, 473, 504, 725, 782, 953
AluBI AGCT 4 cut(s) 459, 675, 761, 785
AluI AGCT 4 cut(s) 459, 675, 761, 785
Alw21I GWGCWC 2 cut(s) 588, 740
Alw26I GTCTC 2 cut(s) 221, 1103
AlwI GGATC 4 cut(s) 446, 459, 617, 1073
Aor51HI AGCGCT 1 cut(s) 383
AoxI GGCC 2 cut(s) 367, 931
ApeKI GCWGC 4 cut(s) 538, 697, 800, 1048
ApoI RAATTY 1 cut(s) 505
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
Asp700I GAANNNNTTC 2 cut(s) 610, 786
AspLEI GCGC 3 cut(s) 347, 384, 420
AspS9I GGNCC 1 cut(s) 368
AsuHPI GGTGA 2 cut(s) 378, 449
BamHI GGATCC 1 cut(s) 451
BbsI GAAGAC 1 cut(s) 125
Bbv12I GWGCWC 2 cut(s) 588, 740
BbvI GCAGC 4 cut(s) 525, 684, 787, 1035
BccI CCATC 7 cut(s) 205, 307, 534, 743, 908, 923, 968
BceAI ACGGC 1 cut(s) 985
BclI TGATCA 1 cut(s) 571
BcoDI GTCTC 2 cut(s) 221, 1103
BfaI CTAG 3 cut(s) 644, 833, 1163
BfmI CTRYAG 1 cut(s) 211
BfoI RGCGCY 1 cut(s) 385
BfuAI ACCTGC 1 cut(s) 218
BisI GCNGC 9 cut(s) 98, 348, 351, 427, 539, 698, 801, 1049, 1143
BlsI GCNGC 9 cut(s) 99, 349, 352, 428, 540, 699, 802, 1050, 1144
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 2 cut(s) 370, 453
BmrI ACTGGG 1 cut(s) 701
BmsI GCATC 1 cut(s) 838
BmuI ACTGGG 1 cut(s) 701
BpiI GAAGAC 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 525
BsaJI CCNNGG 3 cut(s) 447, 942, 1125
BsaXI ACNNNNNCTCC 2 cut(s) 1100, 1130
Bsc4I CCNNNNNNNGG 2 cut(s) 554, 944
Bse118I RCCGGY 1 cut(s) 550
Bse1I ACTGG 2 cut(s) 370, 707
Bse3DI GCAATG 1 cut(s) 1157
Bse8I GATNNNNATC 1 cut(s) 525
BseDI CCNNGG 3 cut(s) 447, 942, 1125
BseGI GGATG 5 cut(s) 299, 526, 574, 691, 979
BseJI GATNNNNATC 1 cut(s) 525
BseLI CCNNNNNNNGG 2 cut(s) 554, 944
BseMI GCAATG 1 cut(s) 1157
BseMII CTCAG 1 cut(s) 1104
BseNI ACTGG 2 cut(s) 370, 707
BseRI GAGGAG 3 cut(s) 128, 163, 1121
BseXI GCAGC 4 cut(s) 525, 684, 787, 1035
BseYI CCCAGC 1 cut(s) 554
Bsh1236I CGCG 1 cut(s) 679
BshFI GGCC 2 cut(s) 369, 933
BsiHKAI GWGCWC 2 cut(s) 588, 740
BsiSI CCGG 1 cut(s) 551
BslI CCNNNNNNNGG 2 cut(s) 554, 944
BsmAI GTCTC 2 cut(s) 221, 1103
BsmI GAATGC 1 cut(s) 167
BsnI GGCC 2 cut(s) 369, 933
Bsp1286I GDGCHC 2 cut(s) 588, 740
Bsp143I GATC 4 cut(s) 451, 571, 622, 1078
Bsp68I TCGCGA 1 cut(s) 679
BspACI CCGC 8 cut(s) 98, 348, 351, 426, 435, 536, 548, 1142
BspANI GGCC 2 cut(s) 369, 933
BspCNI CTCAG 1 cut(s) 1105
BspFNI CGCG 1 cut(s) 679
BspLI GGNNCC 2 cut(s) 370, 453
BspMAI CTGCAG 1 cut(s) 215
BspMI ACCTGC 1 cut(s) 218
BspPI GGATC 4 cut(s) 446, 459, 617, 1073
BspQI GCTCTTC 1 cut(s) 1152
BsrDI GCAATG 1 cut(s) 1157
BsrFI RCCGGY 1 cut(s) 550
BsrI ACTGG 2 cut(s) 370, 707
BssAI RCCGGY 1 cut(s) 550
BssECI CCNNGG 3 cut(s) 447, 942, 1125
BssMI GATC 4 cut(s) 451, 571, 622, 1078
BssT1I CCWWGG 1 cut(s) 1125
Bst4CI ACNGT 2 cut(s) 92, 186
Bst6I CTCTTC 1 cut(s) 1152
BstC8I GCNNGC 4 cut(s) 151, 552, 677, 935
BstDEI CTNAG 2 cut(s) 816, 1113
BstF5I GGATG 5 cut(s) 299, 526, 574, 691, 979
BstFNI CGCG 1 cut(s) 679
BstH2I RGCGCY 1 cut(s) 385
BstHHI GCGC 3 cut(s) 347, 384, 420
BstKTI GATC 4 cut(s) 454, 574, 625, 1081
BstMAI GTCTC 2 cut(s) 221, 1103
BstMBI GATC 4 cut(s) 451, 571, 622, 1078
BstMWI GCNNNNNNNGC 5 cut(s) 103, 426, 432, 547, 806
BstNSI RCATGY 4 cut(s) 67, 829, 963, 1072
BstSFI CTRYAG 1 cut(s) 211
BstUI CGCG 1 cut(s) 679
BstV1I GCAGC 4 cut(s) 525, 684, 787, 1035
BstV2I GAAGAC 1 cut(s) 125
BstX2I RGATCY 2 cut(s) 451, 1078
BstXI CCANNNNNNTGG 1 cut(s) 974
BstYI RGATCY 2 cut(s) 451, 1078
BsuRI GGCC 2 cut(s) 369, 933
BtgZI GCGATG 1 cut(s) 783
BtsCI GGATG 5 cut(s) 299, 526, 574, 691, 979
BtsI GCAGTG 1 cut(s) 436
BtsIMutI CAGTG 3 cut(s) 182, 436, 469
BtuMI TCGCGA 1 cut(s) 679
BveI ACCTGC 1 cut(s) 218
Cac8I GCNNGC 4 cut(s) 151, 552, 677, 935
CfoI GCGC 3 cut(s) 347, 384, 420
Cfr10I RCCGGY 1 cut(s) 550
Cfr13I GGNCC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 836, 906
CviAII CATG 8 cut(s) 64, 163, 575, 589, 826, 960, 988, 1069
CviQI GTAC 2 cut(s) 836, 906
DdeI CTNAG 2 cut(s) 816, 1113
DpnI GATC 4 cut(s) 453, 573, 624, 1080
DpnII GATC 4 cut(s) 451, 571, 622, 1078
Eam1104I CTCTTC 1 cut(s) 1152
EarI CTCTTC 1 cut(s) 1152
EciI GGCGGA 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 1125
Eco47III AGCGCT 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 1125
EcoT22I ATGCAT 2 cut(s) 6, 831
ErhI CCWWGG 1 cut(s) 1125
FaeI CATG 8 cut(s) 67, 166, 578, 592, 829, 963, 991, 1072
FalI AAGNNNNNCTT 4 cut(s) 774, 806, 1131, 1163
FatI CATG 8 cut(s) 63, 162, 574, 588, 825, 959, 987, 1068
FbaI TGATCA 1 cut(s) 571
Fnu4HI GCNGC 9 cut(s) 98, 348, 351, 427, 539, 698, 801, 1049, 1143
FokI GGATG 5 cut(s) 286, 513, 581, 698, 986
Fsp4HI GCNGC 9 cut(s) 98, 348, 351, 427, 539, 698, 801, 1049, 1143
FspBI CTAG 3 cut(s) 644, 833, 1163
GlaI GCGC 3 cut(s) 346, 383, 419
GluI GCNGC 9 cut(s) 98, 348, 351, 427, 539, 698, 801, 1049, 1143
GsaI CCCAGC 1 cut(s) 558
HaeII RGCGCY 1 cut(s) 385
HaeIII GGCC 2 cut(s) 369, 933
HapII CCGG 1 cut(s) 551
HhaI GCGC 3 cut(s) 347, 384, 420
Hin1II CATG 8 cut(s) 67, 166, 578, 592, 829, 963, 991, 1072
Hin6I GCGC 3 cut(s) 345, 382, 418
HinP1I GCGC 3 cut(s) 345, 382, 418
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HinfI GANTC 3 cut(s) 327, 846, 1154
HpaII CCGG 1 cut(s) 551
HphI GGTGA 2 cut(s) 378, 449
Hpy166II GTNNAC 3 cut(s) 189, 712, 980
Hpy188I TCNGA 5 cut(s) 448, 456, 671, 883, 1006
Hpy188III TCNNGA 5 cut(s) 376, 644, 678, 920, 1151
Hpy8I GTNNAC 3 cut(s) 189, 712, 980
HpyAV CCTTC 2 cut(s) 318, 574
HpyCH4III ACNGT 2 cut(s) 92, 186
HpyCH4V TGCA 4 cut(s) 4, 213, 829, 1051
HpyF10VI GCNNNNNNNGC 5 cut(s) 103, 426, 432, 547, 806
HpyF3I CTNAG 2 cut(s) 816, 1113
Hsp92II CATG 8 cut(s) 67, 166, 578, 592, 829, 963, 991, 1072
HspAI GCGC 3 cut(s) 345, 382, 418
KroI GCCGGC 1 cut(s) 550
KroNI GCCGGC 1 cut(s) 552
Ksp22I TGATCA 1 cut(s) 571
Kzo9I GATC 4 cut(s) 451, 571, 622, 1078
LguI GCTCTTC 1 cut(s) 1152
LmnI GCTCC 2 cut(s) 300, 735
Lsp1109I GCAGC 4 cut(s) 525, 684, 787, 1035
LweI GCATC 1 cut(s) 838
MaeI CTAG 3 cut(s) 644, 833, 1163
MaeIII GTNAC 2 cut(s) 357, 875
MalI GATC 4 cut(s) 453, 573, 624, 1080
MboI GATC 4 cut(s) 451, 571, 622, 1078
MflI RGATCY 2 cut(s) 451, 1078
MhlI GDGCHC 2 cut(s) 588, 740
MluCI AATT 8 cut(s) 38, 442, 474, 505, 606, 634, 954, 1053
MlyI GAGTC 1 cut(s) 321
MmeI TCCRAC 4 cut(s) 288, 498, 554, 1053
Mph1103I ATGCAT 2 cut(s) 6, 831
MroNI GCCGGC 1 cut(s) 550
MroXI GAANNNNTTC 2 cut(s) 610, 786
MseI TTAA 4 cut(s) 48, 60, 731, 1119
MslI CAYNNNNRTG 1 cut(s) 972
MspA1I CMGCKG 2 cut(s) 538, 761
MspI CCGG 1 cut(s) 551
Mva1269I GAATGC 1 cut(s) 167
MvnI CGCG 1 cut(s) 679
MwoI GCNNNNNNNGC 5 cut(s) 103, 426, 432, 547, 806
NaeI GCCGGC 1 cut(s) 552
NdeII GATC 4 cut(s) 451, 571, 622, 1078
NgoMIV GCCGGC 1 cut(s) 550
NlaIII CATG 8 cut(s) 67, 166, 578, 592, 829, 963, 991, 1072
NlaIV GGNNCC 2 cut(s) 370, 453
NmuCI GTSAC 1 cut(s) 357
NruI TCGCGA 1 cut(s) 679
NsiI ATGCAT 2 cut(s) 6, 831
NspI RCATGY 4 cut(s) 67, 829, 963, 1072
PaqCI CACCTGC 1 cut(s) 218
PciI ACATGT 3 cut(s) 63, 959, 1068
PciSI GCTCTTC 1 cut(s) 1152
PctI GAATGC 1 cut(s) 167
PdiI GCCGGC 1 cut(s) 552
PdmI GAANNNNTTC 2 cut(s) 610, 786
PfeI GAWTC 2 cut(s) 846, 1154
PkrI GCNGC 9 cut(s) 99, 349, 352, 428, 540, 699, 802, 1050, 1144
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
PscI ACATGT 3 cut(s) 63, 959, 1068
PsiI TTATAA 1 cut(s) 656
PspFI CCCAGC 1 cut(s) 554
PspN4I GGNNCC 2 cut(s) 370, 453
PspPI GGNCC 1 cut(s) 368
PstI CTGCAG 1 cut(s) 215
PsuI RGATCY 2 cut(s) 451, 1078
PvuII CAGCTG 1 cut(s) 761
RruI TCGCGA 1 cut(s) 679
RsaI GTAC 2 cut(s) 837, 907
RsaNI GTAC 2 cut(s) 836, 906
RseI CAYNNNNRTG 1 cut(s) 972
SapI GCTCTTC 1 cut(s) 1152
SaqAI TTAA 4 cut(s) 48, 60, 731, 1119
SatI GCNGC 9 cut(s) 98, 348, 351, 427, 539, 698, 801, 1049, 1143
Sau3AI GATC 4 cut(s) 451, 571, 622, 1078
Sau96I GGNCC 1 cut(s) 368
SchI GAGTC 1 cut(s) 321
SduI GDGCHC 2 cut(s) 588, 740
SetI ASST 9 cut(s) 212, 243, 391, 461, 515, 585, 677, 763, 787
SfaNI GCATC 1 cut(s) 838
SfcI CTRYAG 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 972
Sse9I AATT 8 cut(s) 38, 442, 474, 505, 606, 634, 954, 1053
SsiI CCGC 8 cut(s) 98, 348, 351, 426, 435, 536, 548, 1142
SspI AATATT 2 cut(s) 26, 729
SspMI CTAG 3 cut(s) 644, 833, 1163
StyI CCWWGG 1 cut(s) 1125
TaaI ACNGT 2 cut(s) 92, 186
TaqI TCGA 3 cut(s) 314, 509, 621
TasI AATT 8 cut(s) 38, 442, 474, 505, 606, 634, 954, 1053
TatI WGTACW 1 cut(s) 835
TauI GCSGC 5 cut(s) 100, 350, 353, 429, 1145
TfiI GAWTC 2 cut(s) 846, 1154
Tru1I TTAA 4 cut(s) 48, 60, 731, 1119
Tru9I TTAA 4 cut(s) 48, 60, 731, 1119
TscAI CASTG 3 cut(s) 189, 436, 469
TseFI GTSAC 1 cut(s) 357
TseI GCWGC 4 cut(s) 538, 697, 800, 1048
Tsp45I GTSAC 1 cut(s) 357
TspDTI ATGAA 4 cut(s) 300, 702, 786, 1146
TspGWI ACGGA 2 cut(s) 320, 893
TspRI CASTG 3 cut(s) 189, 436, 469
XapI RAATTY 1 cut(s) 505
XbaI TCTAGA 1 cut(s) 643
XceI RCATGY 4 cut(s) 67, 829, 963, 1072
XmnI GAANNNNTTC 2 cut(s) 610, 786
XspI CTAG 3 cut(s) 644, 833, 1163
Zsp2I ATGCAT 2 cut(s) 6, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.