RchiOBHm_Chr7g0228051

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006657
Physical Location & Seq
Reverse (-)
51376574 .. 51378149
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1137 bp
ATGACAGATTCTCCATGGGCTGTTGTGCCTTACCATGACCCTTATGATCATGAAGTCCCCGACGTTGTGAATTTCATTAATGATACTCCTGATCCAACCCTTGACACATTGTTCGACGGTGGAGTAGTTCCTTCTTTCTCTCATCAATATCCAATTCCTCAGTTTGATAATGGAATCCAAGATTTTCCCATGTCCCCAATGTGGGATATGGGCAACAATTATAATGGCGGAGGGAATAATACTATTTCAGCACTAGCGGATCATCCTTCACAGTGTTCCGGACGCGGAGGAGGAGAATCGGGTTTTGATCAGAATTCGGGGTTGGGGTTTGGGAGTAATGTGATGCCACCTCCAGTTTGGGCTCCACCACCAATCCCCTTCAGTTGCTGCGGTTGCCAAGTTCTAAGGGAAATTGTTCACTTCAATCGCAATTATATTATGACACTTGAAATCCATGGAAGACTCGGTGCCATCTGTCATGCAATTCAAGAAACCCGCGCCAATTACTTGTACGATTCCAGTGCTTCTATTGAGCCTCACTATCAGAACTTTGACTTTTGCAATAAAAACATCGAGGAAGTGAAGCAGTTTCTGACGCAATACTGCTTAGAGCGAAAGGTAGAAGGCTACATTATGCAGCAAGATCCTCTGCAAGCATTTTATCAGACTCTCTGTGTTGGTATGGATGGTGAGTGGGATGATATTCTTAGTTATCCCGACGAATTCATTCCTGAGGACTTGGACACTACCGACGACTTCATTTCTCCTAATTCAGAAATGGCGGAAGAAATCGATGAGGTGACCGTGAATATGAAAGGGAAGGGTCCTAGGCTTCCCAGGTTTTCCCTTGCAGAACAGAGGGAGAGAGCAGCAAAAATGAAATTAAATGATCTTCGTGCTTACTTCCATCTCACCATTGAAGAGGCTTCTGAGCAATTGGGTTTTTGCCCAACTGTTGTCAAGAGGATCTGCCGCAAATTTGGAGTAAGCAGGTGGCCTGCTAGAAAGATCAAAAGCATTGAGAGGCGAATATCAAGCTTGAGGCCGCTTTTGAATTCATATAGTGATAGCACCAGGGTCCATGTTGCAGCTGAAATTAAAAGGCTTGAAAGAGAAGTAGCCCGACTTGTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

378

Amino Acids

42.82

Weight (kDa)

4.92

Isoelectric Point (pI)

51.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 292 - 340 9.8e-22 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 222
AarI CACCTGC 1 cut(s) 981
Acc36I ACCTGC 1 cut(s) 981
AccB1I GGYRCC 1 cut(s) 467
AccII CGCG 2 cut(s) 285, 498
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 8 cut(s) 228, 257, 285, 390, 496, 782, 973, 1046
AclWI GGATC 4 cut(s) 86, 267, 638, 974
AcsI RAATTY 5 cut(s) 70, 313, 722, 977, 1054
AcuI CTGAAG 1 cut(s) 364
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 2 cut(s) 201, 202
AgsI TTSAA 6 cut(s) 424, 449, 488, 920, 1054, 1109
AjnI CCWGG 2 cut(s) 836, 1073
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
AluBI AGCT 2 cut(s) 1038, 1091
AluI AGCT 2 cut(s) 1038, 1091
AlwI GGATC 4 cut(s) 86, 267, 638, 974
AlwNI CAGNNNCTG 2 cut(s) 387, 592
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 2 cut(s) 995, 1043
ApeKI GCWGC 4 cut(s) 387, 637, 869, 1088
ApoI RAATTY 5 cut(s) 70, 313, 722, 977, 1054
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AseI ATTAAT 1 cut(s) 78
Asp700I GAANNNNTTC 2 cut(s) 414, 726
AspA2I CCTAGG 1 cut(s) 827
AspLEI GCGC 1 cut(s) 500
AspS9I GGNCC 2 cut(s) 824, 1078
AsuHPI GGTGA 3 cut(s) 701, 811, 904
AvaII GGWCC 2 cut(s) 824, 1078
AvrII CCTAGG 1 cut(s) 827
AxyI CCTNAGG 1 cut(s) 732
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 466
BbvI GCAGC 4 cut(s) 374, 649, 881, 1100
BccI CCATC 3 cut(s) 479, 680, 915
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 2 cut(s) 838, 1075
BclI TGATCA 2 cut(s) 46, 307
BfaI CTAG 3 cut(s) 254, 828, 1002
BfuAI ACCTGC 1 cut(s) 981
BisI GCNGC 6 cut(s) 388, 638, 870, 973, 1046, 1089
BlnI CCTAGG 1 cut(s) 827
BlsI GCNGC 6 cut(s) 389, 639, 871, 974, 1047, 1090
Bme1390I CCNGG 2 cut(s) 838, 1075
Bme18I GGWCC 2 cut(s) 824, 1078
BmgT120I GGNCC 2 cut(s) 824, 1078
BmiI GGNNCC 4 cut(s) 363, 469, 825, 1079
BmrFI CCNGG 2 cut(s) 838, 1075
BmsI GCATC 1 cut(s) 333
BpiI GAAGAC 1 cut(s) 466
BpmI CTGGAG 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 1060
Bsa29I ATCGAT 1 cut(s) 792
BsaJI CCNNGG 5 cut(s) 14, 454, 827, 836, 1074
BsaWI WCCGGW 1 cut(s) 278
BsaXI ACNNNNNCTCC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 202
Bse1I ACTGG 2 cut(s) 353, 519
Bse21I CCTNAGG 1 cut(s) 732
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 2 cut(s) 838, 1075
BseCI ATCGAT 1 cut(s) 792
BseDI CCNNGG 5 cut(s) 14, 454, 827, 836, 1074
BseGI GGATG 3 cut(s) 262, 691, 703
BseLI CCNNNNNNNGG 2 cut(s) 201, 202
BseMII CTCAG 3 cut(s) 173, 723, 921
BseNI ACTGG 2 cut(s) 353, 519
BseRI GAGGAG 2 cut(s) 303, 306
BseXI GCAGC 4 cut(s) 374, 649, 881, 1100
Bsh1236I CGCG 2 cut(s) 285, 498
BshFI GGCC 2 cut(s) 997, 1045
BshNI GGYRCC 1 cut(s) 467
BshVI ATCGAT 1 cut(s) 792
BsiSI CCGG 1 cut(s) 279
BslFI GGGAC 2 cut(s) 41, 178
BslI CCNNNNNNNGG 2 cut(s) 201, 202
BsmFI GGGAC 2 cut(s) 41, 178
BsnI GGCC 2 cut(s) 997, 1045
Bsp1286I GDGCHC 1 cut(s) 364
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 8 cut(s) 46, 91, 259, 307, 643, 889, 966, 1008
Bsp19I CCATGG 2 cut(s) 14, 454
BspACI CCGC 8 cut(s) 228, 257, 285, 390, 496, 782, 973, 1046
BspANI GGCC 2 cut(s) 997, 1045
BspCNI CTCAG 3 cut(s) 172, 724, 922
BspDI ATCGAT 1 cut(s) 792
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 2 cut(s) 285, 498
BspHI TCATGA 1 cut(s) 49
BspLI GGNNCC 4 cut(s) 363, 469, 825, 1079
BspMI ACCTGC 1 cut(s) 981
BspPI GGATC 4 cut(s) 86, 267, 638, 974
BspT107I GGYRCC 1 cut(s) 467
BsrI ACTGG 2 cut(s) 353, 519
BssECI CCNNGG 5 cut(s) 14, 454, 827, 836, 1074
BssMI GATC 8 cut(s) 46, 91, 259, 307, 643, 889, 966, 1008
BssT1I CCWWGG 3 cut(s) 14, 454, 827
Bst2UI CCWGG 2 cut(s) 838, 1075
Bst4CI ACNGT 4 cut(s) 119, 273, 805, 955
Bst6I CTCTTC 1 cut(s) 915
BstC8I GCNNGC 2 cut(s) 654, 999
BstDEI CTNAG 6 cut(s) 159, 404, 607, 707, 732, 930
BstDSI CCRYGG 2 cut(s) 14, 454
BstEII GGTNACC 1 cut(s) 799
BstF5I GGATG 3 cut(s) 262, 691, 703
BstFNI CGCG 2 cut(s) 285, 498
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 8 cut(s) 49, 94, 262, 310, 646, 892, 969, 1011
BstMBI GATC 8 cut(s) 46, 91, 259, 307, 643, 889, 966, 1008
BstMWI GCNNNNNNNGC 1 cut(s) 393
BstNI CCWGG 2 cut(s) 838, 1075
BstPI GGTNACC 1 cut(s) 799
BstSCI CCNGG 2 cut(s) 836, 1073
BstUI CGCG 2 cut(s) 285, 498
BstV1I GCAGC 4 cut(s) 374, 649, 881, 1100
BstV2I GAAGAC 1 cut(s) 466
BstX2I RGATCY 2 cut(s) 643, 966
BstYI RGATCY 2 cut(s) 643, 966
Bsu15I ATCGAT 1 cut(s) 792
Bsu36I CCTNAGG 1 cut(s) 732
BsuRI GGCC 2 cut(s) 997, 1045
BsuTUI ATCGAT 1 cut(s) 792
BtgI CCRYGG 2 cut(s) 14, 454
BtsCI GGATG 3 cut(s) 262, 691, 703
BtsIMutI CAGTG 2 cut(s) 278, 526
BveI ACCTGC 1 cut(s) 981
Cac8I GCNNGC 2 cut(s) 654, 999
CaiI CAGNNNCTG 2 cut(s) 387, 592
CciI TCATGA 1 cut(s) 49
CfoI GCGC 1 cut(s) 500
Cfr13I GGNCC 2 cut(s) 824, 1078
ClaI ATCGAT 1 cut(s) 792
CseI GACGC 2 cut(s) 291, 604
Csp6I GTAC 1 cut(s) 511
CviAII CATG 7 cut(s) 15, 35, 50, 190, 455, 479, 1082
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 6 cut(s) 159, 404, 607, 707, 732, 930
DpnI GATC 8 cut(s) 48, 93, 261, 309, 645, 891, 968, 1010
DpnII GATC 8 cut(s) 46, 91, 259, 307, 643, 889, 966, 1008
Eam1104I CTCTTC 1 cut(s) 915
EarI CTCTTC 1 cut(s) 915
EciI GGCGGA 2 cut(s) 243, 797
Eco130I CCWWGG 3 cut(s) 14, 454, 827
Eco24I GRGCYC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 824, 1078
Eco57I CTGAAG 1 cut(s) 364
Eco81I CCTNAGG 1 cut(s) 732
Eco91I GGTNACC 1 cut(s) 799
EcoO109I RGGNCCY 1 cut(s) 824
EcoO65I GGTNACC 1 cut(s) 799
EcoRI GAATTC 3 cut(s) 313, 722, 1054
EcoRII CCWGG 2 cut(s) 836, 1073
EcoT14I CCWWGG 3 cut(s) 14, 454, 827
EcoT38I GRGCYC 1 cut(s) 364
ErhI CCWWGG 3 cut(s) 14, 454, 827
FaeI CATG 7 cut(s) 18, 38, 53, 193, 458, 482, 1085
FaqI GGGAC 2 cut(s) 41, 178
FatI CATG 7 cut(s) 14, 34, 49, 189, 454, 478, 1081
FauI CCCGC 1 cut(s) 503
FbaI TGATCA 2 cut(s) 46, 307
Fnu4HI GCNGC 6 cut(s) 388, 638, 870, 973, 1046, 1089
FokI GGATG 3 cut(s) 249, 698, 710
FriOI GRGCYC 1 cut(s) 364
Fsp4HI GCNGC 6 cut(s) 388, 638, 870, 973, 1046, 1089
FspBI CTAG 3 cut(s) 254, 828, 1002
GlaI GCGC 1 cut(s) 499
GluI GCNGC 6 cut(s) 388, 638, 870, 973, 1046, 1089
GsuI CTGGAG 1 cut(s) 336
HaeIII GGCC 2 cut(s) 997, 1045
HapII CCGG 1 cut(s) 279
HgaI GACGC 2 cut(s) 291, 604
HhaI GCGC 1 cut(s) 500
Hin1II CATG 7 cut(s) 18, 38, 53, 193, 458, 482, 1085
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HindIII AAGCTT 1 cut(s) 1036
HinfI GANTC 6 cut(s) 8, 174, 296, 462, 515, 667
HpaII CCGG 1 cut(s) 279
HphI GGTGA 3 cut(s) 701, 811, 904
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 6 cut(s) 312, 546, 594, 666, 775, 931
Hpy188III TCNNGA 7 cut(s) 50, 89, 279, 488, 716, 731, 961
Hpy8I GTNNAC 1 cut(s) 418
Hpy99I CGWCG 4 cut(s) 65, 119, 722, 755
HpyAV CCTTC 5 cut(s) 141, 276, 388, 617, 814
HpyCH4III ACNGT 4 cut(s) 119, 273, 805, 955
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 6 cut(s) 482, 561, 637, 652, 851, 1088
HpyF10VI GCNNNNNNNGC 1 cut(s) 393
HpyF3I CTNAG 6 cut(s) 159, 404, 607, 707, 732, 930
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 7 cut(s) 18, 38, 53, 193, 458, 482, 1085
HspAI GCGC 1 cut(s) 498
Kpn2I TCCGGA 1 cut(s) 278
Ksp22I TGATCA 2 cut(s) 46, 307
Kzo9I GATC 8 cut(s) 46, 91, 259, 307, 643, 889, 966, 1008
LmnI GCTCC 1 cut(s) 367
Lsp1109I GCAGC 4 cut(s) 374, 649, 881, 1100
LweI GCATC 1 cut(s) 333
MaeI CTAG 3 cut(s) 254, 828, 1002
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 799
MalI GATC 8 cut(s) 48, 93, 261, 309, 645, 891, 968, 1010
MboI GATC 8 cut(s) 46, 91, 259, 307, 643, 889, 966, 1008
MboII GAAGA 4 cut(s) 471, 797, 884, 932
MfeI CAATTG 1 cut(s) 935
MflI RGATCY 2 cut(s) 643, 966
MhlI GDGCHC 1 cut(s) 364
MlyI GAGTC 2 cut(s) 456, 661
MmeI TCCRAC 1 cut(s) 119
MroI TCCGGA 1 cut(s) 278
MroXI GAANNNNTTC 2 cut(s) 414, 726
MseI TTAA 3 cut(s) 78, 884, 1098
MspA1I CMGCKG 1 cut(s) 1091
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 2 cut(s) 838, 1075
MunI CAATTG 1 cut(s) 935
MvaI CCWGG 2 cut(s) 838, 1075
MvnI CGCG 2 cut(s) 285, 498
MwoI GCNNNNNNNGC 1 cut(s) 393
NcoI CCATGG 2 cut(s) 14, 454
NdeII GATC 8 cut(s) 46, 91, 259, 307, 643, 889, 966, 1008
NlaIII CATG 7 cut(s) 18, 38, 53, 193, 458, 482, 1085
NlaIV GGNNCC 4 cut(s) 363, 469, 825, 1079
NmuCI GTSAC 1 cut(s) 799
PagI TCATGA 1 cut(s) 49
PaqCI CACCTGC 1 cut(s) 981
PdmI GAANNNNTTC 2 cut(s) 414, 726
PfeI GAWTC 4 cut(s) 8, 174, 296, 515
PkrI GCNGC 6 cut(s) 389, 639, 871, 974, 1047, 1090
PleI GAGTC 2 cut(s) 456, 661
PpsI GAGTC 2 cut(s) 456, 661
PpuMI RGGWCCY 1 cut(s) 824
PshBI ATTAAT 1 cut(s) 78
PsiI TTATAA 1 cut(s) 222
Psp5II RGGWCCY 1 cut(s) 824
Psp6I CCWGG 2 cut(s) 836, 1073
PspEI GGTNACC 1 cut(s) 799
PspGI CCWGG 2 cut(s) 836, 1073
PspN4I GGNNCC 4 cut(s) 363, 469, 825, 1079
PspPI GGNCC 2 cut(s) 824, 1078
PspPPI RGGWCCY 1 cut(s) 824
PstNI CAGNNNCTG 2 cut(s) 387, 592
PsuI RGATCY 2 cut(s) 643, 966
PvuII CAGCTG 1 cut(s) 1091
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
SaqAI TTAA 3 cut(s) 78, 884, 1098
SatI GCNGC 6 cut(s) 388, 638, 870, 973, 1046, 1089
Sau3AI GATC 8 cut(s) 46, 91, 259, 307, 643, 889, 966, 1008
Sau96I GGNCC 2 cut(s) 824, 1078
SchI GAGTC 2 cut(s) 456, 661
ScrFI CCNGG 2 cut(s) 838, 1075
SduI GDGCHC 1 cut(s) 364
SetI ASST 8 cut(s) 66, 352, 621, 801, 842, 995, 1040, 1093
SfaNI GCATC 1 cut(s) 333
SinI GGWCC 2 cut(s) 824, 1078
SmlI CTYRAG 1 cut(s) 1039
SmoI CTYRAG 1 cut(s) 1039
SsiI CCGC 8 cut(s) 228, 257, 285, 390, 496, 782, 973, 1046
SspMI CTAG 3 cut(s) 254, 828, 1002
StyD4I CCNGG 2 cut(s) 836, 1073
StyI CCWWGG 3 cut(s) 14, 454, 827
TaaI ACNGT 4 cut(s) 119, 273, 805, 955
TaiI ACGT 1 cut(s) 66
TaqI TCGA 3 cut(s) 114, 573, 792
TauI GCSGC 2 cut(s) 975, 1048
TfiI GAWTC 4 cut(s) 8, 174, 296, 515
Tru1I TTAA 3 cut(s) 78, 884, 1098
Tru9I TTAA 3 cut(s) 78, 884, 1098
TscAI CASTG 2 cut(s) 278, 526
TseFI GTSAC 1 cut(s) 799
TseI GCWGC 4 cut(s) 387, 637, 869, 1088
Tsp45I GTSAC 1 cut(s) 799
TspDTI ATGAA 7 cut(s) 64, 66, 715, 748, 827, 893, 1047
TspRI CASTG 2 cut(s) 278, 526
VpaK11BI GGWCC 2 cut(s) 824, 1078
VspI ATTAAT 1 cut(s) 78
XapI RAATTY 5 cut(s) 70, 313, 722, 977, 1054
XcmI CCANNNNNNNNNTGG 1 cut(s) 354
XmaJI CCTAGG 1 cut(s) 827
XmnI GAANNNNTTC 2 cut(s) 414, 726
XspI CTAG 3 cut(s) 254, 828, 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.