RchiOBHm_Chr6g0251791

WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005006
Physical Location & Seq
Forward (+)
7068803 .. 7072401
3599 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1218 bp
ATGGCCGACGATCACGACCAAGAAGACGCGGCCGTCGTTGAGGAAGAGTACGCCGTCTGGAAGAAGAACACTCCGTTCCTCTACGACCTCATCATCTCCCACCCTCTCGAATGGCCATCTCTCACCGTCCACTGGGTCCCCTCGGCCCCACAGCCCTACTCCGACCCGTCTCTCGCCGTCCACAAGCTCATCCTCGGGACCCACACCTCCGACGACTACCCCAACTACCTCATGGTCGCCGACGCCCTCCTCCCCGCATCTCAACCCAACGTTGACGCCGTTTCCGACGAGCCCACTCTCCCTAAGGTTGAGATTACGCAGAAGATTCGGGTCGATGGGGAAGTGAATAGGGCAAGGTGTATGCTGCAGAAGCCGAATATGGTTGGGGCGAAAACCAGCGGTAGCGAGGTGTATGTGTTTGATTGTACCAAGCAGGGGCTGAAGTCTCAAGATGGTGAGTGTGATCCTGATTTGAGGTTGAGGGGTCATGACAAAGAAGGGTATGGCTTGTCATGGAGTCCATTTAAGGAGGGTTACCTTTTGAGTGGTTCAAATGATTGTAAAATTTGCTTGTGGGATGTGTCTGCCTTGGCTCAAGATAGAGTGCTTCATCCAATGCATGTTTTTGAGCGTCATGAAAGTGTGGTTAATGATGTGTCGTGGCATTCGAAGAATGAGAATCTGTTTGGGTCTGTTGGGGACGATTGTCAATTGATGATATGGGACTTGAGAACAAATCAAGCCCAAGACTCTGTCAAAGTTCATGAGAAAGAGGTAAACTATCTGTCTTTCCATCCGTGTAATGAGTGGGTTTTGGCTACAGCATCTTCAGACACTACTGTGGGTCTGTTTGATATGCGGAAGCTGACGGTACCTTTGCATGCTCTGAGTGGCCACACAGAGGAGGTATTTCAGGTTGAATGGGACCCTAATCATGATACTGTGTTGGCATCTTATGCTGGTGACCGAAGGTTGATAGTTTGGGACCTTAACAGGATTGGGGATGAGCAGTTGGAGGGAGATGGTGATGATGGGCCACCAGAACTTCTGTTTTCACATGGCGGTCATAAAGCAAAGATATCAGATTTCTCATGGAACAAAGAAGTTCCATGGCTCATTTCAAGTGTAGCTGAAGACAACACTCTCCAGGTCTGGCAAATGGCTGATGGCATATACGGTGACGATGATGGGCAAGAAGGGGAAGCTTCTGATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0031461 GO:0032991 GO:0044424 GO:0044444 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
CAF1C_H4-bd WD40

Protein Analysis

405

Amino Acids

45.4

Weight (kDa)

4.55

Isoelectric Point (pI)

34.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1C_H4-bd PF12265 15 - 85 1.9e-25 Histone-binding protein RBBP4 or subunit C of CAF1 complex
WD40_CDC20-Fz PF24807 111 - 254 2.6e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 129 - 285 4.6e-15 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 142 - 287 5.4e-09 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 144 - 332 4.8e-12 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 162 - 331 1.8e-21 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 163 - 288 6.3e-18 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 168 - 333 6e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_Aladin PF25460 169 - 278 1.3e-07 Aladin seven-bladed propeller
WD40_Prp19 PF24814 171 - 288 2.3e-14 Prp19 WD40 domain
EIF3I PF24805 185 - 290 6.9e-08 EIF3I
WDR55 PF24796 205 - 332 2.8e-09 WDR55
Beta-prop_CAF1B_HIR1 PF24105 207 - 288 4.6e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 211 - 331 5.2e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_EIPR1 PF23609 212 - 284 1.8e-08 EIPR1 beta-propeller
Beta-prop_EIPR1 PF23609 238 - 328 6e-09 EIPR1 beta-propeller
Beta-prop_WDR3_1st PF25173 238 - 388 4.7e-15 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 255 - 331 7.2e-13 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 32
Acc65I GGTACC 1 cut(s) 871
AccB1I GGYRCC 1 cut(s) 871
AccII CGCG 1 cut(s) 29
AciI CCGC 5 cut(s) 29, 255, 399, 859, 1062
AclI AACGTT 1 cut(s) 270
AclWI GGATC 1 cut(s) 458
AcoI YGGCCR 4 cut(s) 3, 30, 113, 892
AcsI RAATTY 1 cut(s) 564
AcuI CTGAAG 3 cut(s) 461, 813, 1152
AcyI GRCGYC 2 cut(s) 243, 276
AfaI GTAC 3 cut(s) 50, 427, 873
AfiI CCNNNNNNNGG 3 cut(s) 132, 435, 901
AgsI TTSAA 3 cut(s) 552, 920, 1122
AjnI CCWGG 1 cut(s) 1146
AleI CACNNNNGTG 1 cut(s) 839
AluBI AGCT 4 cut(s) 187, 865, 1130, 1205
AluI AGCT 4 cut(s) 187, 865, 1130, 1205
Alw26I GTCTC 2 cut(s) 174, 450
AlwI GGATC 1 cut(s) 458
AlwNI CAGNNNCTG 1 cut(s) 439
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 6 cut(s) 3, 30, 113, 144, 892, 1034
ApeKI GCWGC 1 cut(s) 364
ApoI RAATTY 1 cut(s) 564
ArsI GACNNNNNNTTYG 2 cut(s) 859, 891
Asp718I GGTACC 1 cut(s) 871
AspS9I GGNCC 6 cut(s) 136, 145, 198, 925, 985, 1034
AsuHPI GGTGA 5 cut(s) 115, 467, 974, 1037, 1190
AsuII TTCGAA 1 cut(s) 668
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 4 cut(s) 136, 198, 925, 985
AxyI CCTNAGG 1 cut(s) 303
BalI TGGCCA 2 cut(s) 115, 894
BanI GGYRCC 1 cut(s) 871
BanII GRGCYC 1 cut(s) 294
BbsI GAAGAC 2 cut(s) 30, 1140
BbvI GCAGC 1 cut(s) 351
BccI CCATC 8 cut(s) 124, 329, 446, 801, 1016, 1025, 1160, 1181
BceAI ACGGC 4 cut(s) 17, 38, 161, 263
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 1 cut(s) 1148
BcoDI GTCTC 2 cut(s) 174, 450
BfmI CTRYAG 2 cut(s) 365, 819
BisI GCNGC 2 cut(s) 30, 365
BlsI GCNGC 2 cut(s) 31, 366
Bme1390I CCNGG 1 cut(s) 1148
Bme18I GGWCC 4 cut(s) 136, 198, 925, 985
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 6 cut(s) 136, 145, 198, 925, 985, 1034
BmiI GGNNCC 9 cut(s) 137, 138, 147, 199, 200, 873, 926, 927, 986
BmrFI CCNGG 1 cut(s) 1148
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 3 cut(s) 266, 833, 959
BmuI ACTGGG 1 cut(s) 142
BoxI GACNNNNGTC 1 cut(s) 705
BpiI GAAGAC 2 cut(s) 30, 1140
BpmI CTGGAG 1 cut(s) 1130
Bpu14I TTCGAA 1 cut(s) 668
BpuEI CTTGAG 3 cut(s) 432, 579, 748
BsaHI GRCGYC 2 cut(s) 243, 276
BsaJI CCNNGG 4 cut(s) 141, 193, 588, 1109
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 435, 901
Bse1I ACTGG 1 cut(s) 137
Bse21I CCTNAGG 1 cut(s) 303
BseBI CCWGG 1 cut(s) 1148
BseDI CCNNGG 4 cut(s) 141, 193, 588, 1109
BseGI GGATG 5 cut(s) 189, 583, 610, 793, 1009
BseLI CCNNNNNNNGG 3 cut(s) 132, 435, 901
BseMII CTCAG 1 cut(s) 878
BseNI ACTGG 1 cut(s) 137
BseRI GAGGAG 2 cut(s) 239, 917
BseX3I CGGCCG 1 cut(s) 30
BseXI GCAGC 1 cut(s) 351
Bsh1236I CGCG 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 6 cut(s) 5, 32, 115, 146, 894, 1036
BshNI GGYRCC 1 cut(s) 871
BsiEI CGRYCG 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 194
BslFI GGGAC 6 cut(s) 122, 211, 713, 737, 938, 998
BslI CCNNNNNNNGG 3 cut(s) 132, 435, 901
BsmAI GTCTC 2 cut(s) 174, 450
BsmBI CGTCTC 1 cut(s) 174
BsmFI GGGAC 6 cut(s) 122, 211, 713, 737, 938, 998
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 6 cut(s) 5, 32, 115, 146, 894, 1036
BsoBI CYCGRG 1 cut(s) 194
Bsp119I TTCGAA 1 cut(s) 668
Bsp1286I GDGCHC 1 cut(s) 294
Bsp143I GATC 2 cut(s) 10, 463
Bsp19I CCATGG 1 cut(s) 1109
BspACI CCGC 5 cut(s) 29, 255, 399, 859, 1062
BspANI GGCC 6 cut(s) 5, 32, 115, 146, 894, 1036
BspCNI CTCAG 1 cut(s) 879
BspFNI CGCG 1 cut(s) 29
BspHI TCATGA 4 cut(s) 487, 634, 763, 934
BspLI GGNNCC 9 cut(s) 137, 138, 147, 199, 200, 873, 926, 927, 986
BspMAI CTGCAG 1 cut(s) 369
BspPI GGATC 1 cut(s) 458
BspT104I TTCGAA 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 871
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 4 cut(s) 141, 193, 588, 1109
BssMI GATC 2 cut(s) 10, 463
BssNI GRCGYC 2 cut(s) 243, 276
BssT1I CCWWGG 2 cut(s) 588, 1109
Bst2UI CCWGG 1 cut(s) 1148
Bst4CI ACNGT 5 cut(s) 127, 841, 871, 943, 1178
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 2 cut(s) 243, 276
BstAPI GCANNNNNTGC 1 cut(s) 956
BstBI TTCGAA 1 cut(s) 668
BstC8I GCNNGC 1 cut(s) 882
BstDEI CTNAG 2 cut(s) 303, 887
BstDSI CCRYGG 1 cut(s) 1109
BstEII GGTNACC 2 cut(s) 533, 962
BstF5I GGATG 5 cut(s) 189, 583, 610, 793, 1009
BstFNI CGCG 1 cut(s) 29
BstKTI GATC 2 cut(s) 13, 466
BstMAI GTCTC 2 cut(s) 174, 450
BstMBI GATC 2 cut(s) 10, 463
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 2 cut(s) 370, 956
BstNI CCWGG 1 cut(s) 1148
BstNSI RCATGY 2 cut(s) 623, 884
BstPAI GACNNNNGTC 1 cut(s) 705
BstPI GGTNACC 2 cut(s) 533, 962
BstSCI CCNGG 1 cut(s) 1146
BstSFI CTRYAG 2 cut(s) 365, 819
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 351
BstV2I GAAGAC 2 cut(s) 30, 1140
BstZI CGGCCG 1 cut(s) 30
Bsu36I CCTNAGG 1 cut(s) 303
BsuRI GGCC 6 cut(s) 5, 32, 115, 146, 894, 1036
BtgI CCRYGG 1 cut(s) 1109
BtsCI GGATG 5 cut(s) 189, 583, 610, 793, 1009
BtsIMutI CAGTG 1 cut(s) 130
Cac8I GCNNGC 1 cut(s) 882
CaiI CAGNNNCTG 1 cut(s) 439
CciI TCATGA 4 cut(s) 487, 634, 763, 934
Cfr13I GGNCC 6 cut(s) 136, 145, 198, 925, 985, 1034
CseI GACGC 4 cut(s) 35, 251, 284, 620
Csp6I GTAC 3 cut(s) 49, 426, 872
CviQI GTAC 3 cut(s) 49, 426, 872
DdeI CTNAG 2 cut(s) 303, 887
DpnI GATC 2 cut(s) 12, 465
DpnII GATC 2 cut(s) 10, 463
DrdI GACNNNNNNGTC 1 cut(s) 32
DseDI GACNNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 4 cut(s) 3, 30, 113, 892
EagI CGGCCG 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EclXI CGGCCG 1 cut(s) 30
Eco130I CCWWGG 2 cut(s) 588, 1109
Eco24I GRGCYC 1 cut(s) 294
Eco32I GATATC 1 cut(s) 1080
Eco47I GGWCC 4 cut(s) 136, 198, 925, 985
Eco52I CGGCCG 1 cut(s) 30
Eco57I CTGAAG 3 cut(s) 461, 813, 1152
Eco81I CCTNAGG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 194
Eco91I GGTNACC 2 cut(s) 533, 962
EcoO109I RGGNCCY 4 cut(s) 136, 198, 925, 985
EcoO65I GGTNACC 2 cut(s) 533, 962
EcoRII CCWGG 1 cut(s) 1146
EcoRV GATATC 1 cut(s) 1080
EcoT14I CCWWGG 2 cut(s) 588, 1109
EcoT22I ATGCAT 1 cut(s) 621
EcoT38I GRGCYC 1 cut(s) 294
ErhI CCWWGG 2 cut(s) 588, 1109
Esp3I CGTCTC 1 cut(s) 174
FaqI GGGAC 6 cut(s) 122, 211, 713, 737, 938, 998
FauI CCCGC 1 cut(s) 262
Fnu4HI GCNGC 2 cut(s) 30, 365
FokI GGATG 5 cut(s) 176, 590, 597, 780, 1016
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 2 cut(s) 30, 365
GluI GCNGC 2 cut(s) 30, 365
GsuI CTGGAG 1 cut(s) 1130
HaeIII GGCC 6 cut(s) 5, 32, 115, 146, 894, 1036
HgaI GACGC 4 cut(s) 35, 251, 284, 620
Hin1I GRCGYC 2 cut(s) 243, 276
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 1203
HinfI GANTC 4 cut(s) 325, 517, 679, 749
HphI GGTGA 5 cut(s) 115, 467, 974, 1037, 1190
Hpy166II GTNNAC 4 cut(s) 130, 181, 274, 778
Hpy188I TCNGA 8 cut(s) 163, 211, 286, 832, 888, 1084, 1210, 1217
Hpy8I GTNNAC 4 cut(s) 130, 181, 274, 778
Hpy99I CGWCG 5 cut(s) 11, 38, 215, 245, 290
HpyAV CCTTC 3 cut(s) 491, 963, 1190
HpyCH4III ACNGT 5 cut(s) 127, 841, 871, 943, 1178
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 3 cut(s) 367, 619, 880
HpyF10VI GCNNNNNNNGC 2 cut(s) 370, 956
HpyF3I CTNAG 2 cut(s) 303, 887
HpySE526I ACGT 1 cut(s) 270
Hsp92I GRCGYC 2 cut(s) 243, 276
KflI GGGWCCC 3 cut(s) 136, 198, 925
KpnI GGTACC 1 cut(s) 875
Kzo9I GATC 2 cut(s) 10, 463
Lsp1109I GCAGC 1 cut(s) 351
LweI GCATC 3 cut(s) 266, 833, 959
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 3 cut(s) 533, 962, 1178
MalI GATC 2 cut(s) 12, 465
MboI GATC 2 cut(s) 10, 463
MboII GAAGA 8 cut(s) 35, 56, 73, 76, 334, 682, 819, 1145
MfeI CAATTG 1 cut(s) 710
MhlI GDGCHC 1 cut(s) 294
MlsI TGGCCA 2 cut(s) 115, 894
MluCI AATT 2 cut(s) 564, 710
MluNI TGGCCA 2 cut(s) 115, 894
MlyI GAGTC 2 cut(s) 526, 743
MmeI TCCRAC 4 cut(s) 186, 234, 309, 993
Mox20I TGGCCA 2 cut(s) 115, 894
Mph1103I ATGCAT 1 cut(s) 621
MscI TGGCCA 2 cut(s) 115, 894
MseI TTAA 3 cut(s) 525, 648, 990
MslI CAYNNNNRTG 2 cut(s) 639, 839
Msp20I TGGCCA 2 cut(s) 115, 894
MspA1I CMGCKG 1 cut(s) 399
MspR9I CCNGG 1 cut(s) 1148
MunI CAATTG 1 cut(s) 710
Mva1269I GAATGC 1 cut(s) 664
MvaI CCWGG 1 cut(s) 1148
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 2 cut(s) 370, 956
NcoI CCATGG 1 cut(s) 1109
NdeII GATC 2 cut(s) 10, 463
NlaIV GGNNCC 9 cut(s) 137, 138, 147, 199, 200, 873, 926, 927, 986
NmeAIII GCCGAG 1 cut(s) 122
NmuCI GTSAC 2 cut(s) 962, 1178
NsiI ATGCAT 1 cut(s) 621
NspI RCATGY 2 cut(s) 623, 884
NspV TTCGAA 1 cut(s) 668
OliI CACNNNNGTG 1 cut(s) 839
PaeI GCATGC 1 cut(s) 884
PagI TCATGA 4 cut(s) 487, 634, 763, 934
PcsI WCGNNNNNNNCGW 4 cut(s) 33, 276, 282, 665
PctI GAATGC 1 cut(s) 664
PfeI GAWTC 2 cut(s) 325, 679
PflFI GACNNNGTC 1 cut(s) 752
PkrI GCNGC 2 cut(s) 31, 366
PleI GAGTC 2 cut(s) 525, 743
PpsI GAGTC 2 cut(s) 525, 743
PpuMI RGGWCCY 4 cut(s) 136, 198, 925, 985
PshAI GACNNNNGTC 1 cut(s) 705
Psp1406I AACGTT 1 cut(s) 270
Psp5II RGGWCCY 4 cut(s) 136, 198, 925, 985
Psp6I CCWGG 1 cut(s) 1146
PspEI GGTNACC 2 cut(s) 533, 962
PspGI CCWGG 1 cut(s) 1146
PspN4I GGNNCC 9 cut(s) 137, 138, 147, 199, 200, 873, 926, 927, 986
PspPI GGNCC 6 cut(s) 136, 145, 198, 925, 985, 1034
PspPPI RGGWCCY 4 cut(s) 136, 198, 925, 985
PstI CTGCAG 1 cut(s) 369
PstNI CAGNNNCTG 1 cut(s) 439
PsyI GACNNNGTC 1 cut(s) 752
RsaI GTAC 3 cut(s) 50, 427, 873
RsaNI GTAC 3 cut(s) 49, 426, 872
RseI CAYNNNNRTG 2 cut(s) 639, 839
SaqAI TTAA 3 cut(s) 525, 648, 990
SatI GCNGC 2 cut(s) 30, 365
Sau3AI GATC 2 cut(s) 10, 463
Sau96I GGNCC 6 cut(s) 136, 145, 198, 925, 985, 1034
SchI GAGTC 2 cut(s) 526, 743
ScrFI CCNGG 1 cut(s) 1148
SduI GDGCHC 1 cut(s) 294
SfaNI GCATC 3 cut(s) 266, 833, 959
SfcI CTRYAG 2 cut(s) 365, 819
SfuI TTCGAA 1 cut(s) 668
SinI GGWCC 4 cut(s) 136, 198, 925, 985
SmiMI CAYNNNNRTG 2 cut(s) 639, 839
SmlI CTYRAG 3 cut(s) 447, 594, 727
SmoI CTYRAG 3 cut(s) 447, 594, 727
SphI GCATGC 1 cut(s) 884
Sse9I AATT 2 cut(s) 564, 710
SsiI CCGC 5 cut(s) 29, 255, 399, 859, 1062
StyD4I CCNGG 1 cut(s) 1146
StyI CCWWGG 2 cut(s) 588, 1109
TaaI ACNGT 5 cut(s) 127, 841, 871, 943, 1178
TaiI ACGT 1 cut(s) 273
TaqI TCGA 3 cut(s) 108, 333, 668
TaqII GACCGA 1 cut(s) 981
TasI AATT 2 cut(s) 564, 710
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 2 cut(s) 325, 679
Tru1I TTAA 3 cut(s) 525, 648, 990
Tru9I TTAA 3 cut(s) 525, 648, 990
TscAI CASTG 1 cut(s) 137
TseFI GTSAC 2 cut(s) 962, 1178
TseI GCWGC 1 cut(s) 364
Tsp45I GTSAC 2 cut(s) 962, 1178
TspDTI ATGAA 3 cut(s) 599, 651, 752
TspGWI ACGGA 2 cut(s) 63, 786
TspRI CASTG 1 cut(s) 137
Tth111I GACNNNGTC 1 cut(s) 752
VpaK11BI GGWCC 4 cut(s) 136, 198, 925, 985
XapI RAATTY 1 cut(s) 564
XceI RCATGY 2 cut(s) 623, 884
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
Zsp2I ATGCAT 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.