RchiOBHm_Chr6g0301871

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005902
Physical Location & Seq
Forward (+)
62143876 .. 62144450
575 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 444 bp
ATGTCTGGGAGAAAACGTGGAAACGCAATAGTCAGGGAGGAACCAAGAGATGCTTCTATTGAAGCAAGGAGTATTGCTCGTGAAGCGGCGAGAGCTCATCAAATGAGTTTAGCTTCTGGAACTCCGTCGGTCGTCATATCAATGGTCCCTTCGCATGTTTATAGAGTATTTTGGTTGAACCTTCCGAGAAGATTTTGTGATGGTAATCTCCCGGAAACTAAATCGAAACTGACGTTAGAGGATGAGGAAGGCAACGAATTCACGGTTGTCTTTATTCCGGAGCGATATGGGCTGAGTGGAGGGTGGGTGAGCTTTGCGCGGGAAAAAAAGTTGCACGACGGCGACGCCGTAGTGCTTGAACAAATTGAAAAAACTAGACTCAAAGTAAGTCTCCTGGCCCTGAACACAAATATAATTGATCTTACAATGGCTCTTCCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

147

Amino Acids

16.41

Weight (kDa)

7.91

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 47 - 131 4e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 319
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 2 cut(s) 86, 319
AcsI RAATTY 1 cut(s) 257
AcyI GRCGYC 1 cut(s) 345
AgsI TTSAA 4 cut(s) 62, 178, 359, 368
AjnI CCWGG 1 cut(s) 393
AjuI GAANNNNNNNTTGG 2 cut(s) 37, 69
AluBI AGCT 4 cut(s) 95, 113, 312, 441
AluI AGCT 4 cut(s) 95, 113, 312, 441
Alw21I GWGCWC 1 cut(s) 97
Alw26I GTCTC 1 cut(s) 395
Aor13HI TCCGGA 1 cut(s) 277
AoxI GGCC 1 cut(s) 396
ApoI RAATTY 1 cut(s) 257
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 2 cut(s) 145, 397
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 1 cut(s) 319
AvaII GGWCC 1 cut(s) 145
BanII GRGCYC 1 cut(s) 97
BauI CACGAG 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 97
BccI CCATC 1 cut(s) 194
BceAI ACGGC 2 cut(s) 332, 355
BciT130I CCWGG 1 cut(s) 395
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 2 cut(s) 375, 442
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
Bme1390I CCNGG 2 cut(s) 212, 395
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 2 cut(s) 145, 397
BmiI GGNNCC 2 cut(s) 42, 147
BmrFI CCNGG 2 cut(s) 212, 395
BmsI GCATC 1 cut(s) 40
BplI GAGNNNNNCTC 2 cut(s) 61, 93
BpuMI CCSGG 1 cut(s) 212
BsaBI GATNNNNATC 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 345
BsaWI WCCGGW 1 cut(s) 277
BsaXI ACNNNNNCTCC 3 cut(s) 31, 291, 321
Bse8I GATNNNNATC 1 cut(s) 204
BseAI TCCGGA 1 cut(s) 277
BseBI CCWGG 1 cut(s) 395
BseGI GGATG 1 cut(s) 247
BseJI GATNNNNATC 1 cut(s) 204
BseMII CTCAG 1 cut(s) 284
Bsh1236I CGCG 1 cut(s) 319
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 1 cut(s) 398
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 97
BsiSI CCGG 2 cut(s) 212, 278
BslFI GGGAC 1 cut(s) 131
BsmAI GTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 398
Bsp1286I GDGCHC 1 cut(s) 97
Bsp13I TCCGGA 1 cut(s) 277
Bsp143I GATC 1 cut(s) 418
BspACI CCGC 2 cut(s) 86, 319
BspANI GGCC 1 cut(s) 398
BspCNI CTCAG 1 cut(s) 285
BspEI TCCGGA 1 cut(s) 277
BspFNI CGCG 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 42, 147
BspQI GCTCTTC 1 cut(s) 438
BssMI GATC 1 cut(s) 418
BssNI GRCGYC 1 cut(s) 345
BssSI CACGAG 1 cut(s) 78
Bst2BI CACGAG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 395
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 1 cut(s) 438
BstACI GRCGYC 1 cut(s) 345
BstDEI CTNAG 1 cut(s) 293
BstF5I GGATG 1 cut(s) 247
BstFNI CGCG 1 cut(s) 319
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 1 cut(s) 421
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 1 cut(s) 418
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 3 cut(s) 83, 92, 289
BstNI CCWGG 1 cut(s) 395
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 2 cut(s) 210, 393
BstUI CGCG 1 cut(s) 319
BsuRI GGCC 1 cut(s) 398
BtsCI GGATG 1 cut(s) 247
CfoI GCGC 1 cut(s) 319
Cfr13I GGNCC 2 cut(s) 145, 397
CseI GACGC 1 cut(s) 353
CviAII CATG 1 cut(s) 155
CviJI RGCY 7 cut(s) 95, 113, 292, 312, 398, 431, 441
CviKI_1 RGCY 7 cut(s) 95, 113, 292, 312, 398, 431, 441
DdeI CTNAG 1 cut(s) 293
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Ecl136II GAGCTC 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 145
Eco53kI GAGCTC 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 393
EcoT38I GRGCYC 1 cut(s) 97
FaeI CATG 1 cut(s) 158
FaiI YATR 5 cut(s) 137, 156, 162, 288, 413
FalI AAGNNNNNCTT 2 cut(s) 37, 69
FaqI GGGAC 1 cut(s) 131
FatI CATG 1 cut(s) 154
FauI CCCGC 1 cut(s) 312
Fnu4HI GCNGC 1 cut(s) 87
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 97
Fsp4HI GCNGC 1 cut(s) 87
FspBI CTAG 2 cut(s) 375, 442
GlaI GCGC 1 cut(s) 318
GluI GCNGC 1 cut(s) 87
HaeIII GGCC 1 cut(s) 398
HapII CCGG 2 cut(s) 212, 278
HgaI GACGC 1 cut(s) 353
HhaI GCGC 1 cut(s) 319
Hin1I GRCGYC 1 cut(s) 345
Hin1II CATG 1 cut(s) 158
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HinfI GANTC 1 cut(s) 378
HpaII CCGG 2 cut(s) 212, 278
HphI GGTGA 1 cut(s) 319
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 3 cut(s) 80, 117, 278
Hpy99I CGWCG 3 cut(s) 130, 341, 347
HpyAV CCTTC 3 cut(s) 159, 191, 242
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4IV ACGT 2 cut(s) 16, 233
HpyCH4V TGCA 1 cut(s) 334
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 92, 289
HpyF3I CTNAG 1 cut(s) 293
HpySE526I ACGT 2 cut(s) 16, 233
Hsp92I GRCGYC 1 cut(s) 345
Hsp92II CATG 1 cut(s) 158
HspAI GCGC 1 cut(s) 317
Kpn2I TCCGGA 1 cut(s) 277
Kzo9I GATC 1 cut(s) 418
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 1 cut(s) 280
LpnPI CCDG 7 cut(s) 19, 102, 225, 291, 380, 407, 413
LweI GCATC 1 cut(s) 40
MaeI CTAG 2 cut(s) 375, 442
MaeII ACGT 2 cut(s) 16, 233
MalI GATC 1 cut(s) 420
MboI GATC 1 cut(s) 418
MboII GAAGA 2 cut(s) 201, 425
MhlI GDGCHC 1 cut(s) 97
MluCI AATT 3 cut(s) 257, 363, 414
MlyI GAGTC 1 cut(s) 372
MnlI CCTC 4 cut(s) 31, 232, 238, 293
MroI TCCGGA 1 cut(s) 277
MslI CAYNNNNRTG 1 cut(s) 140
MspI CCGG 2 cut(s) 212, 278
MspR9I CCNGG 2 cut(s) 212, 395
MvaI CCWGG 1 cut(s) 395
MvnI CGCG 1 cut(s) 319
MwoI GCNNNNNNNGC 3 cut(s) 83, 92, 289
NciI CCSGG 1 cut(s) 212
NdeII GATC 1 cut(s) 418
NlaIII CATG 1 cut(s) 158
NlaIV GGNNCC 2 cut(s) 42, 147
NspI RCATGY 1 cut(s) 158
PciSI GCTCTTC 1 cut(s) 438
PcsI WCGNNNNNNNCGW 2 cut(s) 230, 345
PfoI TCCNGGA 1 cut(s) 210
PkrI GCNGC 1 cut(s) 88
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
Psp124BI GAGCTC 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 393
PspGI CCWGG 1 cut(s) 393
PspN4I GGNNCC 2 cut(s) 42, 147
PspPI GGNCC 2 cut(s) 145, 397
RseI CAYNNNNRTG 1 cut(s) 140
SacI GAGCTC 1 cut(s) 97
SapI GCTCTTC 1 cut(s) 438
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 1 cut(s) 418
Sau96I GGNCC 2 cut(s) 145, 397
SchI GAGTC 1 cut(s) 372
ScrFI CCNGG 2 cut(s) 212, 395
SduI GDGCHC 1 cut(s) 97
SetI ASST 7 cut(s) 19, 97, 115, 183, 236, 314, 443
SfaNI GCATC 1 cut(s) 40
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 140
Sse9I AATT 3 cut(s) 257, 363, 414
SsiI CCGC 2 cut(s) 86, 319
SspMI CTAG 2 cut(s) 375, 442
SstI GAGCTC 1 cut(s) 97
StyD4I CCNGG 2 cut(s) 210, 393
TaaI ACNGT 1 cut(s) 265
TaiI ACGT 2 cut(s) 19, 236
TaqI TCGA 1 cut(s) 224
TaqII GACCGA 1 cut(s) 118
TasI AATT 3 cut(s) 257, 363, 414
TauI GCSGC 1 cut(s) 89
TspGWI ACGGA 1 cut(s) 114
VpaK11BI GGWCC 1 cut(s) 145
XapI RAATTY 1 cut(s) 257
XceI RCATGY 1 cut(s) 158
XspI CTAG 2 cut(s) 375, 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.