RchiOBHm_Chr2g0092761

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003536
Physical Location & Seq
Forward (+)
6115638 .. 6118230
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 933 bp
ATGGGTACATTTCAGAGGACTGTTCTTACTATGCCAGAAAATGGTATTGGATTGTTTCCAGACATAGGTTTTTCACCTATAGCAGCACAAAAGTCCAGGAGAAGGATCTGTTGGGATGTTGTTCACAATGTCGTGCCTGCTATTGATGAAAGTCTACAAACAGGGGGCTTGATTATGGTTAAAGCTATTAAGCTAATGACATTGTTCAGGCCATCCCAAGTTCAACGGCAAGTCCAATTTTCCAACAATCTCTCCAGTAATGGAACGACAGCAATGAGGAACCTCTTGGACATCATTTATGTGCTCAATCTGATATTCATTCAGCTAGCTCTTGGTATGATGAGGGTGAAGAAACTTTGGATTGCTTCTCTTGGAATGACTGGGAAAAGAATTTCGACGCCATCGGATGTGCTATATGTTGGTCTTGGGACACATTTGGTGCCGTCTCAAAATTTAGGTTCAGTAAAGGAGGCCCTTTTGTCAATTAACTTTTCCCAGGATCCACATGAGGACATCAAAACGATTTTGGCCAAGTTCAACAGTAATCCTGATTCTGAATCCCAACTGAAGCAGTTTTTACCTCAAATAACCTCATCATTTGGTGCAAAAAAGTCAGTTATTGAGACAGTGAACTCTTCAAGAGGTCTTGGAAATGGGGCTCCTTTTTCACTTTTCTTGACACAGAAGTATCTCTTCAAAGTTGCATCTGCTCTTGGGAAGAATGACAATCAATTACCCACGAATCCAAAGTGGAACCCGTCAAGGTTGGAGGAAGTTAATGAGAGCGAAGTGGAAGCTCTGTTCGTACCATTGAGCCCAAACGTGGAGGAGTACTGTGACTGGCATTTTGAAAATGAACACGAGACTGGTTGTGCATGCCATTGGAATATATTGACTGATGATGCAAATAAGATAAACATATATACAAGATAA

Protein Analysis

310

Amino Acids

34.56

Weight (kDa)

7.69

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 124 - 210 1.2e-10 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 439
AccB7I CCANNNNNTGG 1 cut(s) 41
AccI GTMKAC 1 cut(s) 154
AclWI GGATC 3 cut(s) 113, 494, 507
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 2 cut(s) 390, 451
AcuI CTGAAG 1 cut(s) 587
AcyI GRCGYC 1 cut(s) 398
AfaI GTAC 3 cut(s) 7, 807, 833
AfiI CCNNNNNNNGG 4 cut(s) 41, 65, 102, 823
AgsI TTSAA 5 cut(s) 224, 538, 639, 697, 851
AjnI CCWGG 2 cut(s) 95, 495
AjuI GAANNNNNNNTTGG 4 cut(s) 30, 62, 94, 126
AloI GAACNNNNNNTCC 2 cut(s) 785, 817
AluBI AGCT 5 cut(s) 185, 193, 325, 329, 797
AluI AGCT 5 cut(s) 185, 193, 325, 329, 797
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 3 cut(s) 450, 617, 857
AlwI GGATC 3 cut(s) 113, 494, 507
AoxI GGCC 3 cut(s) 209, 471, 528
ApeKI GCWGC 1 cut(s) 83
ApoI RAATTY 2 cut(s) 390, 451
ArsI GACNNNNNNTTYG 2 cut(s) 830, 862
AspS9I GGNCC 1 cut(s) 472
AsuHPI GGTGA 2 cut(s) 66, 358
AsuNHI GCTAGC 1 cut(s) 325
BaeI ACNNNNGTAYC 2 cut(s) 671, 704
BalI TGGCCA 1 cut(s) 530
BamHI GGATCC 1 cut(s) 499
BanI GGYRCC 1 cut(s) 439
BanII GRGCYC 2 cut(s) 661, 818
BauI CACGAG 1 cut(s) 860
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 95
BccI CCATC 2 cut(s) 220, 409
BceAI ACGGC 2 cut(s) 242, 427
BciT130I CCWGG 2 cut(s) 97, 497
BcoDI GTCTC 3 cut(s) 450, 617, 857
BfaI CTAG 1 cut(s) 326
BfmI CTRYAG 1 cut(s) 78
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
BmcAI AGTACT 1 cut(s) 833
Bme1390I CCNGG 2 cut(s) 97, 497
BmgT120I GGNCC 1 cut(s) 472
BmiI GGNNCC 5 cut(s) 281, 441, 501, 660, 755
BmrFI CCNGG 2 cut(s) 97, 497
BmrI ACTGGG 1 cut(s) 390
BmsI GCATC 2 cut(s) 713, 892
BmtI GCTAGC 1 cut(s) 329
BmuI ACTGGG 1 cut(s) 390
BpmI CTGGAG 1 cut(s) 238
BsaHI GRCGYC 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 495
BsaXI ACNNNNNCTCC 2 cut(s) 236, 266
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 65, 102, 823
Bse1I ACTGG 4 cut(s) 255, 385, 845, 871
Bse3DI GCAATG 1 cut(s) 279
BseBI CCWGG 2 cut(s) 97, 497
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 3 cut(s) 121, 212, 412
BseLI CCNNNNNNNGG 4 cut(s) 41, 65, 102, 823
BseMI GCAATG 1 cut(s) 279
BseNI ACTGG 4 cut(s) 255, 385, 845, 871
BseRI GAGGAG 1 cut(s) 842
BseXI GCAGC 1 cut(s) 95
BshFI GGCC 3 cut(s) 211, 473, 530
BshNI GGYRCC 1 cut(s) 439
BsiHKAI GWGCWC 1 cut(s) 306
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 4 cut(s) 41, 65, 102, 823
BsmAI GTCTC 3 cut(s) 450, 617, 857
BsmBI CGTCTC 1 cut(s) 450
BsmFI GGGAC 1 cut(s) 442
BsnI GGCC 3 cut(s) 211, 473, 530
Bsp1286I GDGCHC 3 cut(s) 306, 661, 818
Bsp143I GATC 2 cut(s) 105, 499
BspANI GGCC 3 cut(s) 211, 473, 530
BspLI GGNNCC 5 cut(s) 281, 441, 501, 660, 755
BspOI GCTAGC 1 cut(s) 329
BspPI GGATC 3 cut(s) 113, 494, 507
BspT107I GGYRCC 1 cut(s) 439
BsrDI GCAATG 1 cut(s) 279
BsrI ACTGG 4 cut(s) 255, 385, 845, 871
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 2 cut(s) 105, 499
BssNI GRCGYC 1 cut(s) 398
BssSI CACGAG 1 cut(s) 860
Bst2BI CACGAG 1 cut(s) 860
Bst2UI CCWGG 2 cut(s) 97, 497
Bst4CI ACNGT 4 cut(s) 22, 542, 628, 836
Bst6I CTCTTC 2 cut(s) 640, 698
BstACI GRCGYC 1 cut(s) 398
BstC8I GCNNGC 3 cut(s) 138, 327, 877
BstF5I GGATG 3 cut(s) 121, 212, 412
BstKTI GATC 2 cut(s) 108, 502
BstMAI GTCTC 3 cut(s) 450, 617, 857
BstMBI GATC 2 cut(s) 105, 499
BstNI CCWGG 2 cut(s) 97, 497
BstNSI RCATGY 1 cut(s) 879
BstSCI CCNGG 2 cut(s) 95, 495
BstSFI CTRYAG 1 cut(s) 78
BstV1I GCAGC 1 cut(s) 95
BstX2I RGATCY 2 cut(s) 105, 499
BstYI RGATCY 2 cut(s) 105, 499
BsuRI GGCC 3 cut(s) 211, 473, 530
BtsCI GGATG 3 cut(s) 121, 212, 412
BtsIMutI CAGTG 1 cut(s) 633
Cac8I GCNNGC 3 cut(s) 138, 327, 877
Cfr13I GGNCC 1 cut(s) 472
CseI GACGC 1 cut(s) 406
Csp6I GTAC 3 cut(s) 6, 806, 832
CviAII CATG 2 cut(s) 506, 876
CviQI GTAC 3 cut(s) 6, 806, 832
DpnI GATC 2 cut(s) 107, 501
DpnII GATC 2 cut(s) 105, 499
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 2 cut(s) 640, 698
EarI CTCTTC 2 cut(s) 640, 698
Eco24I GRGCYC 2 cut(s) 661, 818
Eco57I CTGAAG 1 cut(s) 587
EcoO109I RGGNCCY 1 cut(s) 472
EcoRII CCWGG 2 cut(s) 95, 495
EcoT38I GRGCYC 2 cut(s) 661, 818
Esp3I CGTCTC 1 cut(s) 450
FaeI CATG 2 cut(s) 509, 879
FalI AAGNNNNNCTT 2 cut(s) 677, 709
FaqI GGGAC 1 cut(s) 442
FatI CATG 2 cut(s) 505, 875
FblI GTMKAC 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 3 cut(s) 128, 199, 419
FriOI GRGCYC 2 cut(s) 661, 818
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 1 cut(s) 326
GluI GCNGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 238
HaeIII GGCC 3 cut(s) 211, 473, 530
HgaI GACGC 1 cut(s) 406
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 2 cut(s) 509, 879
HinfI GANTC 3 cut(s) 551, 557, 742
HphI GGTGA 2 cut(s) 66, 358
Hpy166II GTNNAC 3 cut(s) 124, 155, 631
Hpy188I TCNGA 4 cut(s) 15, 312, 406, 556
Hpy188III TCNNGA 4 cut(s) 59, 548, 639, 676
Hpy8I GTNNAC 3 cut(s) 124, 155, 631
Hpy99I CGWCG 1 cut(s) 400
HpyAV CCTTC 1 cut(s) 96
HpyCH4III ACNGT 4 cut(s) 22, 542, 628, 836
HpyCH4IV ACGT 1 cut(s) 822
HpyCH4V TGCA 4 cut(s) 605, 704, 875, 905
HpySE526I ACGT 1 cut(s) 822
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 2 cut(s) 509, 879
Kzo9I GATC 2 cut(s) 105, 499
LmnI GCTCC 1 cut(s) 664
Lsp1109I GCAGC 1 cut(s) 95
LweI GCATC 2 cut(s) 713, 892
MaeI CTAG 1 cut(s) 326
MaeII ACGT 1 cut(s) 822
MaeIII GTNAC 1 cut(s) 836
MalI GATC 2 cut(s) 107, 501
MboI GATC 2 cut(s) 105, 499
MboII GAAGA 4 cut(s) 361, 627, 685, 730
MflI RGATCY 2 cut(s) 105, 499
MhlI GDGCHC 3 cut(s) 306, 661, 818
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 5 cut(s) 236, 390, 451, 483, 731
MluNI TGGCCA 1 cut(s) 530
MmeI TCCRAC 2 cut(s) 267, 747
Mox20I TGGCCA 1 cut(s) 530
MscI TGGCCA 1 cut(s) 530
MseI TTAA 4 cut(s) 180, 189, 486, 777
MslI CAYNNNNRTG 1 cut(s) 299
Msp20I TGGCCA 1 cut(s) 530
MspR9I CCNGG 2 cut(s) 97, 497
MvaI CCWGG 2 cut(s) 97, 497
NdeII GATC 2 cut(s) 105, 499
NheI GCTAGC 1 cut(s) 325
NlaIII CATG 2 cut(s) 509, 879
NlaIV GGNNCC 5 cut(s) 281, 441, 501, 660, 755
NmuCI GTSAC 1 cut(s) 836
NspI RCATGY 1 cut(s) 879
PaeI GCATGC 1 cut(s) 879
PfeI GAWTC 3 cut(s) 551, 557, 742
PflMI CCANNNNNTGG 1 cut(s) 41
PfoI TCCNGGA 1 cut(s) 95
PkrI GCNGC 1 cut(s) 85
Psp6I CCWGG 2 cut(s) 95, 495
PspGI CCWGG 2 cut(s) 95, 495
PspN4I GGNNCC 5 cut(s) 281, 441, 501, 660, 755
PspPI GGNCC 1 cut(s) 472
PsuI RGATCY 2 cut(s) 105, 499
RsaI GTAC 3 cut(s) 7, 807, 833
RsaNI GTAC 3 cut(s) 6, 806, 832
RseI CAYNNNNRTG 1 cut(s) 299
SaqAI TTAA 4 cut(s) 180, 189, 486, 777
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 105, 499
Sau96I GGNCC 1 cut(s) 472
ScaI AGTACT 1 cut(s) 833
ScrFI CCNGG 2 cut(s) 97, 497
SduI GDGCHC 3 cut(s) 306, 661, 818
SfaNI GCATC 2 cut(s) 713, 892
SfcI CTRYAG 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 299
SphI GCATGC 1 cut(s) 879
Sse9I AATT 5 cut(s) 236, 390, 451, 483, 731
SspMI CTAG 1 cut(s) 326
StyD4I CCNGG 2 cut(s) 95, 495
TaaI ACNGT 4 cut(s) 22, 542, 628, 836
TaiI ACGT 1 cut(s) 825
TaqI TCGA 1 cut(s) 395
TasI AATT 5 cut(s) 236, 390, 451, 483, 731
TatI WGTACW 1 cut(s) 831
TfiI GAWTC 3 cut(s) 551, 557, 742
Tru1I TTAA 4 cut(s) 180, 189, 486, 777
Tru9I TTAA 4 cut(s) 180, 189, 486, 777
TscAI CASTG 1 cut(s) 633
TseFI GTSAC 1 cut(s) 836
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 1 cut(s) 836
TspDTI ATGAA 3 cut(s) 162, 307, 870
TspRI CASTG 1 cut(s) 633
Van91I CCANNNNNTGG 1 cut(s) 41
XapI RAATTY 2 cut(s) 390, 451
XceI RCATGY 1 cut(s) 879
XmiI GTMKAC 1 cut(s) 154
XspI CTAG 1 cut(s) 326
ZrmI AGTACT 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.