RchiOBHm_Chr2g0101271

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002237
Physical Location & Seq
Reverse (-)
12908645 .. 12913523
4879 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2286 bp
ATGGGTACTGATGCTGCTGAAGTAGTAGGGACAGAGAGAAAGCCTAATTCCTTTAAGGTTCACCGTTCAATGGGCATTGAACTTTGGAAGTTGGTGGAGAGAATTTCAAAGATATTTCCCGACATAGAAGCAGCAAGACCTCGTTGCTCAACGGGAATACAGGCTCTATGCATGTTGAATCGTGCAATTGAGAAAGCCAATCAACTTGTTCAGTATTGCAGCGAGTCTAGCAAACTCTACCTGGCGTTTACGGGGGATGTGATAGTCTCAAGATGTCAAAAATCAAGGAACTTGTTGGAGCAAAGTTTGTGCCAGATTCAGAGTTGGGTTCCGGTGATTTTGGTGGCAGAGATCTCTAAAATAGTTGATGATCTTAAGGCTGCAACATTTAGACTGCATCCATCTGAAGAAGAGGCTGGGAAGGTCATCCGAGAATTGCTCCACCAAGATGCTTCTGCATCAAATTCAATAAAAGATTCTGAACTCAAGGCTCTTCAACTTGCATCTTTGAGGCTCCATATTACCACATCAAAGGAAGTCTTAATAGAGAAAAGATCTATTAAGAAGTTGCTACAAAAAGTTGGTGACAGTGACCCTCGAAAAAAGCAGATCTTGACATATTTCTTGTATCTATTGAAGAAGTATGGAAGCTTAATATTGGTAAAGCAAACAGAGAGTGCCCCTGTGTGGCAACAAGGATCACTTGCTTCTGAGAATTCTGGCAACGACTTTGGATATAATCAGTGTGCTGAAGTTGAACCTCACACTGGTTATGGGGAATATGAGGCACAAAGTAACATGTTGCTTGGAGCTGTACCTCCTCAAGCATTTTTGTGCGGGATATCTTCCAGGTTGATGTACGATCCTGTTGTCATTGCTTCTGGACAAACATATGAAAGGATGTGGATACAGAAGTGGTTTAATGAGGGAAATGATACATGCCCAAAAACTAATACCAAACTGGCCCACCTTTCATTGACTCCAAACGTTGGCATGAAGGAGTTGATATCAAGGTGGTGTACGGAGTCTGGAGTCACCATCCCCAACCCAAGTATGCAACCAGAAGCACTTGGCTCATGGGAAAACTCTTCCACTTCCATTGCTAGTTTAGGCAGTTCTATGAATGATCTACGTCTACAAATGGATCTCAGCACTATTTCATTTGGATCCTTAGATACCAGTTTCAATTCTAAGACAGAAGATGGCTCAAATCTGGTGCAGATTGATGATGATTCTCTGAAATACCGATCTGCTAAAAAATGCGAGACAGATTTGGAATTTCTATCTAATATTAGTGAGCTCAAATGGGATTCCCAACGCAAGGCTGTTGAAGATGTTAAAAACGATTTGAAACGCAATCCTCAAGCTTCTTATGCTATGTCCTCTAAGAATTTTGTTGAACCTCTCATCAAATTTTTGAGGGATGCATATGATCAGGATGATGTAGAGGCTCAGAGAGATGGATTTAAATTATTATCTACCTTTGTCAGCAAAAACAGAAACGGGATATCTTATTTAGGTGAAGACGTGTATAGTCTGTTAGTATCTTCTCTTGATTCAGAGGTAAAGGAAGAAGGTCTTGCCATAATGGAAATTCTGTCTGTCCACCAGGACTGCAGAGTTAACATCTCATCATCTGGTGCACTTACATCCATTCTAAAACTTCTAGACTCTCAGAGCAGAAGTATACAAGGAAAAGCCCTTAAAATTTTGCACAATTTGTCCTTCGATAGAGATATTTGTTCAGAGATGGTATCTTTGGAGTGCATCCCAAAATTGGTTCCTTTCTTTGAAGACAGTGCTCTTACTGGAAACTGTATCGCTATATTGAAAAATCTGAGCGATTATGAAGGGGCCAGGATTTCTATTGCTGAAACTGATGGATGCATTGCTTATGTTGTTGTAGTCCTAGACACTGGCAGTAAAGAGCATCAAGAGCATGCTGTTGATATCCTTCTTTCTTTATGCTCTCAGCGCATTGACTATTGTAACTTGGTCATGCATGAGGGTGTAATTCCTGCTCTTGTCCTCTCATCTGCCAATGGGACAGAGAGAACAAGGATAAACGCAACGGAAGTTCTACGTCTCTTGAGAGATATTGATTATGACGATGAGCAAGAATCTGCTGAACCTGAGCCTGAGCCTGAGGCAGCTAGAGATGAGCTACCTAGAGACAATGGAAATCACTCGAACGGGAAGAAAGCTTCTAAGGCATCAGGATTTTTTGGAAAGAAGTTATCAATATTCTCAAAACCGAGTTCTCTTTCACCGAAAAAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KAP U-box

Protein Analysis

761

Amino Acids

84.54

Weight (kDa)

5.57

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 274 - 342 2.9e-17 U-box domain
ARM_PUB PF25598 452 - 699 1.4e-14 Plant U-box protein ARM repeat region
KAP PF05804 486 - 644 1e-07 Kinesin-associated protein (KAP)
Arm_2 PF04826 506 - 610 3e-07 Armadillo-like
ARM_ARMC5 PF24768 533 - 676 8.9e-07 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 989
AccI GTMKAC 2 cut(s) 1135, 1687
AciI CCGC 1 cut(s) 837
AclI AACGTT 1 cut(s) 987
AclWI GGATC 5 cut(s) 706, 857, 1152, 1161, 1174
AcsI RAATTY 8 cut(s) 102, 463, 715, 1277, 1390, 1412, 1593, 1707
AcuI CTGAAG 3 cut(s) 39, 426, 771
AfaI GTAC 4 cut(s) 7, 816, 860, 1021
AfiI CCNNNNNNNGG 6 cut(s) 70, 687, 767, 989, 1321, 1777
AflII CTTAAG 1 cut(s) 374
AflIII ACRYGT 2 cut(s) 798, 1527
AjiI CACGTC 1 cut(s) 1528
AjnI CCWGG 4 cut(s) 240, 848, 1608, 1856
AluBI AGCT 7 cut(s) 651, 812, 1300, 1367, 2153, 2164, 2204
AluI AGCT 7 cut(s) 651, 812, 1300, 1367, 2153, 2164, 2204
Alw21I GWGCWC 3 cut(s) 1302, 1645, 1804
Alw26I GTCTC 4 cut(s) 271, 1259, 2090, 2166
Alw44I GTGCAC 1 cut(s) 1641
AlwI GGATC 5 cut(s) 706, 857, 1152, 1161, 1174
AoxI GGCC 2 cut(s) 963, 1854
ApaLI GTGCAC 1 cut(s) 1641
ApeKI GCWGC 5 cut(s) 14, 131, 219, 380, 2150
ApoI RAATTY 8 cut(s) 102, 463, 715, 1277, 1390, 1412, 1593, 1707
AspLEI GCGC 1 cut(s) 1977
AspS9I GGNCC 2 cut(s) 964, 1854
AsuHPI GGTGA 6 cut(s) 53, 346, 596, 1027, 1532, 2259
AxyI CCTNAGG 1 cut(s) 2145
BaeGI GKGCMC 2 cut(s) 682, 1645
BaeI ACNNNNGTAYC 2 cut(s) 899, 932
BamHI GGATCC 1 cut(s) 1166
BanII GRGCYC 1 cut(s) 1302
BbsI GAAGAC 2 cut(s) 1530, 1800
Bbv12I GWGCWC 3 cut(s) 1302, 1645, 1804
BbvI GCAGC 4 cut(s) 143, 231, 367, 2162
BccI CCATC 6 cut(s) 409, 1046, 1196, 1454, 1744, 1874
BciT130I CCWGG 4 cut(s) 242, 850, 1610, 1858
BciVI GTATCC 1 cut(s) 900
BclI TGATCA 1 cut(s) 1432
BcoDI GTCTC 4 cut(s) 271, 1259, 2090, 2166
BfaI CTAG 6 cut(s) 228, 1104, 1667, 1910, 2154, 2169
BfmI CTRYAG 1 cut(s) 1615
BfrI CTTAAG 1 cut(s) 374
BfuI GTATCC 1 cut(s) 900
BglII AGATCT 3 cut(s) 351, 554, 609
BisI GCNGC 5 cut(s) 15, 132, 220, 381, 2151
BlsI GCNGC 5 cut(s) 16, 133, 221, 382, 2152
Bme1390I CCNGG 4 cut(s) 242, 850, 1610, 1858
BmgBI CACGTC 1 cut(s) 1528
BmgT120I GGNCC 2 cut(s) 964, 1854
BmiI GGNNCC 5 cut(s) 330, 515, 1168, 1782, 1855
BmrFI CCNGG 4 cut(s) 242, 850, 1610, 1858
BmsI GCATC 9 cut(s) 406, 439, 467, 512, 1414, 1776, 1874, 1939, 2222
BpiI GAAGAC 2 cut(s) 1530, 1800
BplI GAGNNNNNCTC 2 cut(s) 423, 455
BpmI CTGGAG 1 cut(s) 1050
Bpu10I CCTNAGC 2 cut(s) 2133, 2139
BpuEI CTTGAG 5 cut(s) 253, 470, 807, 1347, 2110
BsaWI WCCGGW 1 cut(s) 331
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 687, 767, 989, 1321, 1777
Bse1I ACTGG 5 cut(s) 772, 966, 1179, 1813, 1921
Bse21I CCTNAGG 1 cut(s) 2145
Bse3DI GCAATG 3 cut(s) 873, 1098, 1887
BseBI CCWGG 4 cut(s) 242, 850, 1610, 1858
BseLI CCNNNNNNNGG 6 cut(s) 70, 687, 767, 989, 1321, 1777
BseMI GCAATG 3 cut(s) 873, 1098, 1887
BseMII CTCAG 9 cut(s) 702, 1162, 1466, 1688, 1829, 1985, 2124, 2130, 2136
BseNI ACTGG 5 cut(s) 772, 966, 1179, 1813, 1921
BseRI GAGGAG 1 cut(s) 810
BseSI GKGCMC 2 cut(s) 682, 1645
BseXI GCAGC 4 cut(s) 143, 231, 367, 2162
BseYI CCCAGC 1 cut(s) 416
BsgI GTGCAG 1 cut(s) 1238
BshFI GGCC 2 cut(s) 965, 1856
BsiHKAI GWGCWC 3 cut(s) 1302, 1645, 1804
BsiSI CCGG 1 cut(s) 332
BslFI GGGAC 2 cut(s) 43, 2059
BslI CCNNNNNNNGG 6 cut(s) 70, 687, 767, 989, 1321, 1777
BsmAI GTCTC 4 cut(s) 271, 1259, 2090, 2166
BsmBI CGTCTC 1 cut(s) 2090
BsmFI GGGAC 2 cut(s) 43, 2059
BsnI GGCC 2 cut(s) 965, 1856
Bsp1286I GDGCHC 4 cut(s) 682, 1302, 1645, 1804
BspACI CCGC 1 cut(s) 837
BspANI GGCC 2 cut(s) 965, 1856
BspCNI CTCAG 9 cut(s) 703, 1161, 1465, 1687, 1830, 1984, 2125, 2131, 2137
BspLI GGNNCC 5 cut(s) 330, 515, 1168, 1782, 1855
BspMAI CTGCAG 1 cut(s) 1619
BspPI GGATC 5 cut(s) 706, 857, 1152, 1161, 1174
BspQI GCTCTTC 1 cut(s) 498
BspTI CTTAAG 1 cut(s) 374
BsrDI GCAATG 3 cut(s) 873, 1098, 1887
BsrI ACTGG 5 cut(s) 772, 966, 1179, 1813, 1921
BssNAI GTATAC 1 cut(s) 1688
Bst1107I GTATAC 1 cut(s) 1688
Bst2UI CCWGG 4 cut(s) 242, 850, 1610, 1858
Bst4CI ACNGT 4 cut(s) 65, 590, 1799, 1817
Bst6I CTCTTC 3 cut(s) 405, 498, 1093
BstAFI CTTAAG 1 cut(s) 374
BstC8I GCNNGC 1 cut(s) 1941
BstHHI GCGC 1 cut(s) 1977
BstMAI GTCTC 4 cut(s) 271, 1259, 2090, 2166
BstMWI GCNNNNNNNGC 5 cut(s) 228, 1373, 1936, 1974, 2210
BstNI CCWGG 4 cut(s) 242, 850, 1610, 1858
BstNSI RCATGY 4 cut(s) 175, 802, 942, 1943
BstSCI CCNGG 4 cut(s) 240, 848, 1608, 1856
BstSFI CTRYAG 1 cut(s) 1615
BstSLI GKGCMC 2 cut(s) 682, 1645
BstV1I GCAGC 4 cut(s) 143, 231, 367, 2162
BstV2I GAAGAC 2 cut(s) 1530, 1800
BstX2I RGATCY 5 cut(s) 351, 554, 609, 1144, 1166
BstYI RGATCY 5 cut(s) 351, 554, 609, 1144, 1166
BstZ17I GTATAC 1 cut(s) 1688
Bsu36I CCTNAGG 1 cut(s) 2145
BsuI GTATCC 1 cut(s) 900
BsuRI GGCC 2 cut(s) 965, 1856
BtrI CACGTC 1 cut(s) 1528
BtsIMutI CAGTG 5 cut(s) 595, 749, 765, 1804, 1914
Cac8I GCNNGC 1 cut(s) 1941
CfoI GCGC 1 cut(s) 1977
Cfr13I GGNCC 2 cut(s) 964, 1854
Csp6I GTAC 4 cut(s) 6, 815, 859, 1020
CspCI CAANNNNNGTGG 2 cut(s) 1081, 1116
CviAII CATG 8 cut(s) 172, 799, 939, 994, 1077, 1940, 1999, 2003
CviQI GTAC 4 cut(s) 6, 815, 859, 1020
DraI TTTAAA 1 cut(s) 1468
Eam1104I CTCTTC 3 cut(s) 405, 498, 1093
EarI CTCTTC 3 cut(s) 405, 498, 1093
Ecl136II GAGCTC 1 cut(s) 1300
Eco24I GRGCYC 1 cut(s) 1302
Eco32I GATATC 4 cut(s) 843, 1008, 1509, 1951
Eco53kI GAGCTC 1 cut(s) 1300
Eco57I CTGAAG 3 cut(s) 39, 426, 771
Eco81I CCTNAGG 1 cut(s) 2145
EcoICRI GAGCTC 1 cut(s) 1300
EcoRI GAATTC 1 cut(s) 715
EcoRII CCWGG 4 cut(s) 240, 848, 1608, 1856
EcoRV GATATC 4 cut(s) 843, 1008, 1509, 1951
EcoT22I ATGCAT 4 cut(s) 173, 1429, 1889, 2004
EcoT38I GRGCYC 1 cut(s) 1302
Esp3I CGTCTC 1 cut(s) 2090
FaeI CATG 8 cut(s) 175, 802, 942, 997, 1080, 1943, 2002, 2006
FalI AAGNNNNNCTT 8 cut(s) 524, 556, 596, 628, 687, 719, 1563, 1595
FaqI GGGAC 2 cut(s) 43, 2059
FatI CATG 8 cut(s) 171, 798, 938, 993, 1076, 1939, 1998, 2002
FauI CCCGC 1 cut(s) 830
FauNDI CATATG 2 cut(s) 892, 1429
FbaI TGATCA 1 cut(s) 1432
FblI GTMKAC 2 cut(s) 1135, 1687
Fnu4HI GCNGC 5 cut(s) 15, 132, 220, 381, 2151
FriOI GRGCYC 1 cut(s) 1302
Fsp4HI GCNGC 5 cut(s) 15, 132, 220, 381, 2151
FspBI CTAG 6 cut(s) 228, 1104, 1667, 1910, 2154, 2169
GlaI GCGC 1 cut(s) 1976
GluI GCNGC 5 cut(s) 15, 132, 220, 381, 2151
GsaI CCCAGC 1 cut(s) 420
GsuI CTGGAG 1 cut(s) 1050
HaeIII GGCC 2 cut(s) 965, 1856
HapII CCGG 1 cut(s) 332
HhaI GCGC 1 cut(s) 1977
Hin1II CATG 8 cut(s) 175, 802, 942, 997, 1080, 1943, 2002, 2006
Hin6I GCGC 1 cut(s) 1975
HinP1I GCGC 1 cut(s) 1975
HincII GTYRAC 1 cut(s) 1624
HindII GTYRAC 1 cut(s) 1624
HindIII AAGCTT 3 cut(s) 649, 1365, 2202
HpaI GTTAAC 1 cut(s) 1624
HpaII CCGG 1 cut(s) 332
HphI GGTGA 6 cut(s) 53, 346, 596, 1027, 1532, 2259
Hpy166II GTNNAC 8 cut(s) 61, 249, 1020, 1136, 1606, 1624, 1643, 1688
Hpy8I GTNNAC 8 cut(s) 61, 249, 1020, 1136, 1606, 1624, 1643, 1688
HpyAV CCTTC 6 cut(s) 415, 991, 1568, 1735, 1844, 1964
HpyCH4III ACNGT 4 cut(s) 65, 590, 1799, 1817
HpyCH4IV ACGT 4 cut(s) 987, 1132, 1527, 2083
HpyF10VI GCNNNNNNNGC 5 cut(s) 228, 1373, 1936, 1974, 2210
HpySE526I ACGT 4 cut(s) 987, 1132, 1527, 2083
Hsp92II CATG 8 cut(s) 175, 802, 942, 997, 1080, 1943, 2002, 2006
HspAI GCGC 1 cut(s) 1975
Ksp22I TGATCA 1 cut(s) 1432
KspAI GTTAAC 1 cut(s) 1624
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 4 cut(s) 298, 444, 519, 809
Lsp1109I GCAGC 4 cut(s) 143, 231, 367, 2162
LweI GCATC 9 cut(s) 406, 439, 467, 512, 1414, 1776, 1874, 1939, 2222
MaeI CTAG 6 cut(s) 228, 1104, 1667, 1910, 2154, 2169
MaeII ACGT 4 cut(s) 987, 1132, 1527, 2083
MaeIII GTNAC 5 cut(s) 584, 590, 794, 1033, 1988
MfeI CAATTG 1 cut(s) 186
MflI RGATCY 5 cut(s) 351, 554, 609, 1144, 1166
MhlI GDGCHC 4 cut(s) 682, 1302, 1645, 1804
MlyI GAGTC 5 cut(s) 233, 973, 1034, 1041, 1664
MmeI TCCRAC 1 cut(s) 276
Mph1103I ATGCAT 4 cut(s) 173, 1429, 1889, 2004
MslI CAYNNNNRTG 3 cut(s) 447, 832, 2007
MspCI CTTAAG 1 cut(s) 374
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 4 cut(s) 242, 850, 1610, 1858
MunI CAATTG 1 cut(s) 186
MvaI CCWGG 4 cut(s) 242, 850, 1610, 1858
MwoI GCNNNNNNNGC 5 cut(s) 228, 1373, 1936, 1974, 2210
NdeI CATATG 2 cut(s) 892, 1429
NlaIII CATG 8 cut(s) 175, 802, 942, 997, 1080, 1943, 2002, 2006
NlaIV GGNNCC 5 cut(s) 330, 515, 1168, 1782, 1855
NmuCI GTSAC 3 cut(s) 584, 590, 1033
NsiI ATGCAT 4 cut(s) 173, 1429, 1889, 2004
NspI RCATGY 4 cut(s) 175, 802, 942, 1943
PaeI GCATGC 1 cut(s) 1943
PciI ACATGT 1 cut(s) 798
PciSI GCTCTTC 1 cut(s) 498
PfeI GAWTC 7 cut(s) 178, 316, 476, 1232, 1310, 1556, 2120
PflMI CCANNNNNTGG 1 cut(s) 989
PkrI GCNGC 5 cut(s) 16, 133, 221, 382, 2152
PleI GAGTC 5 cut(s) 232, 973, 1033, 1040, 1664
PpsI GAGTC 5 cut(s) 232, 973, 1033, 1040, 1664
PscI ACATGT 1 cut(s) 798
Psp124BI GAGCTC 1 cut(s) 1302
Psp1406I AACGTT 1 cut(s) 987
Psp6I CCWGG 4 cut(s) 240, 848, 1608, 1856
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 4 cut(s) 240, 848, 1608, 1856
PspN4I GGNNCC 5 cut(s) 330, 515, 1168, 1782, 1855
PspPI GGNCC 2 cut(s) 964, 1854
PstI CTGCAG 1 cut(s) 1619
PsuI RGATCY 5 cut(s) 351, 554, 609, 1144, 1166
RsaI GTAC 4 cut(s) 7, 816, 860, 1021
RsaNI GTAC 4 cut(s) 6, 815, 859, 1020
RseI CAYNNNNRTG 3 cut(s) 447, 832, 2007
SacI GAGCTC 1 cut(s) 1302
SapI GCTCTTC 1 cut(s) 498
SatI GCNGC 5 cut(s) 15, 132, 220, 381, 2151
Sau96I GGNCC 2 cut(s) 964, 1854
SchI GAGTC 5 cut(s) 233, 973, 1034, 1041, 1664
ScrFI CCNGG 4 cut(s) 242, 850, 1610, 1858
SduI GDGCHC 4 cut(s) 682, 1302, 1645, 1804
SfaNI GCATC 9 cut(s) 406, 439, 467, 512, 1414, 1776, 1874, 1939, 2222
SfcI CTRYAG 1 cut(s) 1615
SmiI ATTTAAAT 1 cut(s) 1468
SmiMI CAYNNNNRTG 3 cut(s) 447, 832, 2007
SmlI CTYRAG 6 cut(s) 268, 374, 485, 822, 1362, 2089
SmoI CTYRAG 6 cut(s) 268, 374, 485, 822, 1362, 2089
SphI GCATGC 1 cut(s) 1943
SsiI CCGC 1 cut(s) 837
SspI AATATT 3 cut(s) 657, 1291, 2244
SspMI CTAG 6 cut(s) 228, 1104, 1667, 1910, 2154, 2169
SstI GAGCTC 1 cut(s) 1302
StyD4I CCNGG 4 cut(s) 240, 848, 1608, 1856
SwaI ATTTAAAT 1 cut(s) 1468
TaaI ACNGT 4 cut(s) 65, 590, 1799, 1817
TaiI ACGT 4 cut(s) 990, 1135, 1530, 2086
TaqI TCGA 3 cut(s) 598, 1728, 2189
TfiI GAWTC 7 cut(s) 178, 316, 476, 1232, 1310, 1556, 2120
TscAI CASTG 5 cut(s) 595, 749, 772, 1804, 1921
TseFI GTSAC 3 cut(s) 584, 590, 1033
TseI GCWGC 5 cut(s) 14, 131, 219, 380, 2150
Tsp45I GTSAC 3 cut(s) 584, 590, 1033
TspDTI ATGAA 6 cut(s) 909, 963, 1010, 1136, 1149, 1863
TspGWI ACGGA 2 cut(s) 1037, 2087
TspRI CASTG 5 cut(s) 595, 749, 772, 1804, 1921
Van91I CCANNNNNTGG 1 cut(s) 989
Vha464I CTTAAG 1 cut(s) 374
VneI GTGCAC 1 cut(s) 1641
XapI RAATTY 8 cut(s) 102, 463, 715, 1277, 1390, 1412, 1593, 1707
XbaI TCTAGA 1 cut(s) 1666
XceI RCATGY 4 cut(s) 175, 802, 942, 1943
XmiI GTMKAC 2 cut(s) 1135, 1687
XspI CTAG 6 cut(s) 228, 1104, 1667, 1910, 2154, 2169
Zsp2I ATGCAT 4 cut(s) 173, 1429, 1889, 2004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.