RchiOBHm_Chr2g0153851

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006945
Physical Location & Seq
Forward (+)
71114006 .. 71115841
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1632 bp
ATGCTTGCAGGGTACACCAAGAATAGGGAAATGTGTTTCGGGTTGAGGTTTTTTGCGGAGATGCTGGCGAGGAACGTGGTTTTGTGGAATTTGATGTTGGATGGGTTTGTGCAGATTCCAGACCCGAATATTGTTTCGTGGGTTACAATGTTGTGTGGGTTTGCGCAAAATGGGAAGATTGCGCAGGCAGGGGACCTCTTTGAGCAGACACCAAGTAGAAATGTGGTTGCTTGGAATGCAATGCTTGCAGGCTATGTTCAAGACCACCAAATTGACAAGGCTGTGAAAATTTTTAGGGATATGCCAGAGACGGATTCTGTATCATGGACTACAATGATCAATGGGTATGTTAGAGTTGGCAAGCTTGACGAAGCAAGGGAATTGCTAAACTGGATGCCATACAAGAACATTGCAGCACAAACAGCAATGATATCTGGATGTGCTCATAATGGAAGGATGGATGAAGCAAGTCAGATCTTCAATCAGATTGCTATTCGTGATGGTAAAAATGCTAACAAGGATTCTTGGAATACTATGATAACTGGTTATGCTCAAGTAGGGGAGATGGATAAAGCCCTTCAAATCTTTGAGGAGATGGGCGACAAGAACATAGTTCCTTGGAATTCTCTAATTACAGGTTATGTGCAGAACGGGTTATATCTGGATGCACTTAAGAGTATTGTGATCTTGGGACAGGAAAGAAAGAGGCCTGATCAGTCAACTTTTTCATGTGGTCTAAGTGCATATGCTAATCTTGCAGATTTACAAGTGGGACGGCAACTTCACCATTTGGTTGTGAAGACTGGCTATCTAAATGATTTGTTTGTCAGCAGTGCTCTGATTACGATGTATGCTAAATGTGGAACGGTTGCAGTAGAGCTCTTTGAAGAGATGCTAATAGAAGGGGTGAATCCAGATCAACTGACCTTTGTTGGGATGTTGTCTGCATGCAGTCACAGTGGGCTGGTTGATTGGGGATTAAAATTGTTTAAGAGCATGACTCAAGTTAACCTTATTGAACCTCTAGCTGAACACTATGCTTGCATGGTAGACTTGCTTGGCCGTGCAGGGAGGTTAGAGGAAGCCTTTGAAATAGTGAGGGATATGAAGATCAAGGCAACTGCAAGAATGTGGGGTGCATTACTTGGAGCTTCCAGGATACACCGGAATCTAAAGTTTGCCAATTATGCTACGAAGAAACTTTTAGAACTCAAACCTGATAAAACTTCAAATTTTGTGCTATTGTCAAACATGAATGCTGAGGCAGGCAGATGGGATGAGGTTGAAATAGTCAGTATGTTGATTAACCAACGTAAAATGGCCCCAGCCGCAGTTGGGAATCATGCTATTCTCTTTGATGATCCAGTGCAACCCAGAACAGCAGAGATATGCAGTGTACTGAACTCCCTAGCCAGAGAGATGAAAAACACTAGTTCCTTTGATGAATGTTTTACCACCTACAGATACGATAAATGGTACGCCTTTTCACTTTTCTTAAACTGCTACATTTTAGCACTTTGTAAATTATCAGACTCTTTTGTTAGTGCATATACAAATTATTGGGGTCCTCTTGGTAGAGAACTTGTACATTTCCATGGACATGGCAGGAAAGAAAAAAAATGTTCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

543

Amino Acids

61.13

Weight (kDa)

6.83

Isoelectric Point (pI)

29.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 73 - 103 6.4e-07 PPR repeat family
PPR_2 PF13041 105 - 137 5.6e-08 PPR repeat family
PPR PF01535 107 - 130 2.2e-06 PPR repeat
PPR_1 PF12854 172 - 199 8.5e-06 PPR repeat
PPR_2 PF13041 175 - 206 5.1e-08 PPR repeat family
PPR PF01535 175 - 204 9.6e-09 PPR repeat
E_motif PF20431 388 - 435 6.6e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 165, 183
AccI GTMKAC 1 cut(s) 1050
AciI CCGC 2 cut(s) 56, 1329
AclWI GGATC 1 cut(s) 1355
AcoI YGGCCR 1 cut(s) 1060
AcsI RAATTY 4 cut(s) 88, 288, 622, 1231
AfaI GTAC 4 cut(s) 14, 1398, 1478, 1587
AfiI CCNNNNNNNGG 3 cut(s) 24, 933, 1335
AflII CTTAAG 1 cut(s) 671
AgsI TTSAA 8 cut(s) 260, 481, 581, 887, 1019, 1091, 1230, 1286
AhlI ACTAGT 1 cut(s) 1430
AjnI CCWGG 1 cut(s) 1154
AjuI GAANNNNNNNTTGG 2 cut(s) 80, 112
AluBI AGCT 4 cut(s) 364, 880, 1028, 1151
AluI AGCT 4 cut(s) 364, 880, 1028, 1151
Alw21I GWGCWC 3 cut(s) 445, 838, 882
Alw26I GTCTC 1 cut(s) 302
AlwI GGATC 1 cut(s) 1355
AoxI GGCC 3 cut(s) 707, 1060, 1320
ApeKI GCWGC 1 cut(s) 413
ApoI RAATTY 4 cut(s) 88, 288, 622, 1231
AspLEI GCGC 2 cut(s) 166, 184
AspS9I GGNCC 3 cut(s) 193, 1321, 1565
AsuHPI GGTGA 2 cut(s) 776, 919
AvaII GGWCC 2 cut(s) 193, 1565
BanII GRGCYC 1 cut(s) 882
BbsI GAAGAC 1 cut(s) 806
Bbv12I GWGCWC 3 cut(s) 445, 838, 882
BbvCI CCTCAGC 1 cut(s) 1260
BbvI GCAGC 1 cut(s) 425
BccI CCATC 6 cut(s) 95, 451, 494, 559, 589, 1266
BceAI ACGGC 2 cut(s) 791, 1047
BciT130I CCWGG 1 cut(s) 1156
BciVI GTATCC 1 cut(s) 1152
BclI TGATCA 2 cut(s) 336, 712
BcoDI GTCTC 1 cut(s) 302
BcuI ACTAGT 1 cut(s) 1430
BfaI CTAG 3 cut(s) 1025, 1409, 1431
BfmI CTRYAG 1 cut(s) 1459
BfrI CTTAAG 1 cut(s) 671
BfuI GTATCC 1 cut(s) 1152
BglII AGATCT 1 cut(s) 474
BisI GCNGC 2 cut(s) 414, 1329
BlsI GCNGC 2 cut(s) 415, 1330
Bme1390I CCNGG 1 cut(s) 1156
Bme18I GGWCC 2 cut(s) 193, 1565
BmgT120I GGNCC 3 cut(s) 193, 1321, 1565
BmiI GGNNCC 3 cut(s) 194, 1323, 1566
BmrFI CCNGG 1 cut(s) 1156
BmsI GCATC 4 cut(s) 51, 384, 655, 882
BpiI GAAGAC 1 cut(s) 806
BplI GAGNNNNNCTC 2 cut(s) 985, 1017
Bpu10I CCTNAGC 1 cut(s) 1260
BpuEI CTTGAG 2 cut(s) 537, 987
BsaJI CCNNGG 2 cut(s) 617, 1594
BsaWI WCCGGW 1 cut(s) 1164
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 933, 1335
Bse1I ACTGG 4 cut(s) 395, 547, 808, 1364
Bse3DI GCAATG 3 cut(s) 246, 408, 432
BseBI CCWGG 1 cut(s) 1156
BseDI CCNNGG 2 cut(s) 617, 1594
BseGI GGATG 8 cut(s) 106, 399, 443, 462, 466, 670, 942, 1282
BseLI CCNNNNNNNGG 3 cut(s) 24, 933, 1335
BseMI GCAATG 3 cut(s) 246, 408, 432
BseMII CTCAG 1 cut(s) 1251
BseNI ACTGG 4 cut(s) 395, 547, 808, 1364
BseRI GAGGAG 1 cut(s) 605
BseXI GCAGC 1 cut(s) 425
BseYI CCCAGC 1 cut(s) 1324
BsgI GTGCAG 3 cut(s) 131, 665, 1086
BshFI GGCC 3 cut(s) 709, 1062, 1322
BsiHKAI GWGCWC 3 cut(s) 445, 838, 882
BsiSI CCGG 1 cut(s) 1165
BslFI GGGAC 3 cut(s) 206, 705, 786
BslI CCNNNNNNNGG 3 cut(s) 24, 933, 1335
BsmAI GTCTC 1 cut(s) 302
BsmBI CGTCTC 1 cut(s) 302
BsmFI GGGAC 3 cut(s) 206, 705, 786
BsmI GAATGC 2 cut(s) 241, 1261
BsnI GGCC 3 cut(s) 709, 1062, 1322
Bsp1286I GDGCHC 3 cut(s) 445, 838, 882
Bsp1407I TGTACA 1 cut(s) 1585
Bsp143I GATC 7 cut(s) 336, 474, 684, 712, 916, 1110, 1360
Bsp19I CCATGG 1 cut(s) 1594
BspACI CCGC 2 cut(s) 56, 1329
BspANI GGCC 3 cut(s) 709, 1062, 1322
BspCNI CTCAG 1 cut(s) 1252
BspLI GGNNCC 3 cut(s) 194, 1323, 1566
BspPI GGATC 1 cut(s) 1355
BspTI CTTAAG 1 cut(s) 671
BsrDI GCAATG 3 cut(s) 246, 408, 432
BsrGI TGTACA 1 cut(s) 1585
BsrI ACTGG 4 cut(s) 395, 547, 808, 1364
BssECI CCNNGG 2 cut(s) 617, 1594
BssMI GATC 7 cut(s) 336, 474, 684, 712, 916, 1110, 1360
BssT1I CCWWGG 2 cut(s) 617, 1594
Bst2UI CCWGG 1 cut(s) 1156
Bst4CI ACNGT 2 cut(s) 868, 959
Bst6I CTCTTC 1 cut(s) 882
BstAFI CTTAAG 1 cut(s) 671
BstAPI GCANNNNNTGC 1 cut(s) 245
BstAUI TGTACA 1 cut(s) 1585
BstC8I GCNNGC 9 cut(s) 6, 66, 186, 246, 250, 362, 949, 1042, 1267
BstDEI CTNAG 2 cut(s) 737, 1260
BstDSI CCRYGG 1 cut(s) 1594
BstF5I GGATG 8 cut(s) 106, 399, 443, 462, 466, 670, 942, 1282
BstHHI GCGC 2 cut(s) 166, 184
BstKTI GATC 7 cut(s) 339, 477, 687, 715, 919, 1113, 1363
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 7 cut(s) 336, 474, 684, 712, 916, 1110, 1360
BstMWI GCNNNNNNNGC 6 cut(s) 236, 245, 422, 755, 1187, 1328
BstNI CCWGG 1 cut(s) 1156
BstNSI RCATGY 1 cut(s) 951
BstSCI CCNGG 1 cut(s) 1154
BstSFI CTRYAG 1 cut(s) 1459
BstV1I GCAGC 1 cut(s) 425
BstV2I GAAGAC 1 cut(s) 806
BstX2I RGATCY 1 cut(s) 474
BstXI CCANNNNNNTGG 1 cut(s) 1601
BstYI RGATCY 1 cut(s) 474
BsuI GTATCC 1 cut(s) 1152
BsuRI GGCC 3 cut(s) 709, 1062, 1322
BtgI CCRYGG 1 cut(s) 1594
BtsCI GGATG 8 cut(s) 106, 399, 443, 462, 466, 670, 942, 1282
BtsI GCAGTG 2 cut(s) 838, 1399
BtsIMutI CAGTG 4 cut(s) 838, 964, 1371, 1399
Cac8I GCNNGC 9 cut(s) 6, 66, 186, 246, 250, 362, 949, 1042, 1267
CfoI GCGC 2 cut(s) 166, 184
Cfr13I GGNCC 3 cut(s) 193, 1321, 1565
Csp6I GTAC 4 cut(s) 13, 1397, 1477, 1586
CviAII CATG 9 cut(s) 324, 729, 948, 997, 1045, 1252, 1343, 1595, 1601
CviQI GTAC 4 cut(s) 13, 1397, 1477, 1586
DdeI CTNAG 2 cut(s) 737, 1260
DpnI GATC 7 cut(s) 338, 476, 686, 714, 918, 1112, 1362
DpnII GATC 7 cut(s) 336, 474, 684, 712, 916, 1110, 1360
EaeI YGGCCR 1 cut(s) 1060
Eam1104I CTCTTC 1 cut(s) 882
EarI CTCTTC 1 cut(s) 882
Ecl136II GAGCTC 1 cut(s) 880
Eco130I CCWWGG 2 cut(s) 617, 1594
Eco147I AGGCCT 1 cut(s) 709
Eco24I GRGCYC 1 cut(s) 882
Eco32I GATATC 1 cut(s) 432
Eco47I GGWCC 2 cut(s) 193, 1565
Eco53kI GAGCTC 1 cut(s) 880
EcoICRI GAGCTC 1 cut(s) 880
EcoO109I RGGNCCY 2 cut(s) 193, 1565
EcoRI GAATTC 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 1154
EcoRV GATATC 1 cut(s) 432
EcoT14I CCWWGG 2 cut(s) 617, 1594
EcoT38I GRGCYC 1 cut(s) 882
ErhI CCWWGG 2 cut(s) 617, 1594
Esp3I CGTCTC 1 cut(s) 302
FaeI CATG 9 cut(s) 327, 732, 951, 1000, 1048, 1255, 1346, 1598, 1604
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FaqI GGGAC 3 cut(s) 206, 705, 786
FatI CATG 9 cut(s) 323, 728, 947, 996, 1044, 1251, 1342, 1594, 1600
FauNDI CATATG 1 cut(s) 745
FbaI TGATCA 2 cut(s) 336, 712
FblI GTMKAC 1 cut(s) 1050
Fnu4HI GCNGC 2 cut(s) 414, 1329
FokI GGATG 8 cut(s) 113, 406, 450, 469, 473, 677, 949, 1289
FriOI GRGCYC 1 cut(s) 882
Fsp4HI GCNGC 2 cut(s) 414, 1329
FspBI CTAG 3 cut(s) 1025, 1409, 1431
FspI TGCGCA 2 cut(s) 165, 183
GlaI GCGC 2 cut(s) 165, 183
GluI GCNGC 2 cut(s) 414, 1329
GsaI CCCAGC 1 cut(s) 1328
HaeIII GGCC 3 cut(s) 709, 1062, 1322
HapII CCGG 1 cut(s) 1165
HhaI GCGC 2 cut(s) 166, 184
Hin1II CATG 9 cut(s) 327, 732, 951, 1000, 1048, 1255, 1346, 1598, 1604
Hin6I GCGC 2 cut(s) 164, 182
HinP1I GCGC 2 cut(s) 164, 182
HincII GTYRAC 2 cut(s) 720, 1009
HindII GTYRAC 2 cut(s) 720, 1009
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 8 cut(s) 115, 314, 521, 910, 1000, 1168, 1339, 1532
HpaI GTTAAC 1 cut(s) 1009
HpaII CCGG 1 cut(s) 1165
HphI GGTGA 2 cut(s) 776, 919
Hpy166II GTNNAC 5 cut(s) 15, 720, 1009, 1051, 1397
Hpy188I TCNGA 4 cut(s) 474, 486, 840, 1531
Hpy188III TCNNGA 6 cut(s) 119, 260, 435, 497, 662, 914
Hpy8I GTNNAC 5 cut(s) 15, 720, 1009, 1051, 1397
HpyAV CCTTC 3 cut(s) 447, 587, 896
HpyCH4III ACNGT 2 cut(s) 868, 959
HpyCH4IV ACGT 2 cut(s) 75, 1312
HpyF10VI GCNNNNNNNGC 6 cut(s) 236, 245, 422, 755, 1187, 1328
HpyF3I CTNAG 2 cut(s) 737, 1260
HpySE526I ACGT 2 cut(s) 75, 1312
Hsp92II CATG 9 cut(s) 327, 732, 951, 1000, 1048, 1255, 1346, 1598, 1604
HspAI GCGC 2 cut(s) 164, 182
Ksp22I TGATCA 2 cut(s) 336, 712
KspAI GTTAAC 1 cut(s) 1009
Kzo9I GATC 7 cut(s) 336, 474, 684, 712, 916, 1110, 1360
LmnI GCTCC 1 cut(s) 1148
Lsp1109I GCAGC 1 cut(s) 425
LweI GCATC 4 cut(s) 51, 384, 655, 882
MaeI CTAG 3 cut(s) 1025, 1409, 1431
MaeII ACGT 2 cut(s) 75, 1312
MaeIII GTNAC 2 cut(s) 142, 953
MalI GATC 7 cut(s) 338, 476, 686, 714, 918, 1112, 1362
MboI GATC 7 cut(s) 336, 474, 684, 712, 916, 1110, 1360
MboII GAAGA 6 cut(s) 187, 469, 811, 899, 1120, 1207
MflI RGATCY 1 cut(s) 474
MhlI GDGCHC 3 cut(s) 445, 838, 882
MlyI GAGTC 2 cut(s) 994, 1526
MmeI TCCRAC 1 cut(s) 78
MseI TTAA 6 cut(s) 672, 980, 990, 1008, 1305, 1496
MslI CAYNNNNRTG 2 cut(s) 1593, 1599
MspCI CTTAAG 1 cut(s) 671
MspI CCGG 1 cut(s) 1165
MspR9I CCNGG 1 cut(s) 1156
Mva1269I GAATGC 2 cut(s) 241, 1261
MvaI CCWGG 1 cut(s) 1156
MwoI GCNNNNNNNGC 6 cut(s) 236, 245, 422, 755, 1187, 1328
NcoI CCATGG 1 cut(s) 1594
NdeI CATATG 1 cut(s) 745
NdeII GATC 7 cut(s) 336, 474, 684, 712, 916, 1110, 1360
NlaIII CATG 9 cut(s) 327, 732, 951, 1000, 1048, 1255, 1346, 1598, 1604
NlaIV GGNNCC 3 cut(s) 194, 1323, 1566
NmuCI GTSAC 1 cut(s) 953
NsbI TGCGCA 2 cut(s) 165, 183
NspI RCATGY 1 cut(s) 951
PaeI GCATGC 1 cut(s) 951
PceI AGGCCT 1 cut(s) 709
PctI GAATGC 2 cut(s) 241, 1261
PfeI GAWTC 6 cut(s) 115, 314, 521, 910, 1168, 1339
PfoI TCCNGGA 1 cut(s) 1154
PkrI GCNGC 2 cut(s) 415, 1330
PleI GAGTC 2 cut(s) 994, 1526
PpsI GAGTC 2 cut(s) 994, 1526
PpuMI RGGWCCY 2 cut(s) 193, 1565
Psp124BI GAGCTC 1 cut(s) 882
Psp5II RGGWCCY 2 cut(s) 193, 1565
Psp6I CCWGG 1 cut(s) 1154
PspFI CCCAGC 1 cut(s) 1324
PspGI CCWGG 1 cut(s) 1154
PspN4I GGNNCC 3 cut(s) 194, 1323, 1566
PspPI GGNCC 3 cut(s) 193, 1321, 1565
PspPPI RGGWCCY 2 cut(s) 193, 1565
PsuI RGATCY 1 cut(s) 474
RsaI GTAC 4 cut(s) 14, 1398, 1478, 1587
RsaNI GTAC 4 cut(s) 13, 1397, 1477, 1586
RseI CAYNNNNRTG 2 cut(s) 1593, 1599
SacI GAGCTC 1 cut(s) 882
SaqAI TTAA 6 cut(s) 672, 980, 990, 1008, 1305, 1496
SatI GCNGC 2 cut(s) 414, 1329
Sau3AI GATC 7 cut(s) 336, 474, 684, 712, 916, 1110, 1360
Sau96I GGNCC 3 cut(s) 193, 1321, 1565
SchI GAGTC 2 cut(s) 994, 1526
ScrFI CCNGG 1 cut(s) 1156
SduI GDGCHC 3 cut(s) 445, 838, 882
SfaNI GCATC 4 cut(s) 51, 384, 655, 882
SfcI CTRYAG 1 cut(s) 1459
SinI GGWCC 2 cut(s) 193, 1565
SmiMI CAYNNNNRTG 2 cut(s) 1593, 1599
SmlI CTYRAG 3 cut(s) 552, 671, 1002
SmoI CTYRAG 3 cut(s) 552, 671, 1002
SpeI ACTAGT 1 cut(s) 1430
SphI GCATGC 1 cut(s) 951
SseBI AGGCCT 1 cut(s) 709
SsiI CCGC 2 cut(s) 56, 1329
SspI AATATT 1 cut(s) 130
SspMI CTAG 3 cut(s) 1025, 1409, 1431
SstI GAGCTC 1 cut(s) 882
StuI AGGCCT 1 cut(s) 709
StyD4I CCNGG 1 cut(s) 1154
StyI CCWWGG 2 cut(s) 617, 1594
TaaI ACNGT 2 cut(s) 868, 959
TaiI ACGT 2 cut(s) 78, 1315
TatI WGTACW 2 cut(s) 1396, 1585
TauI GCSGC 1 cut(s) 1331
TfiI GAWTC 6 cut(s) 115, 314, 521, 910, 1168, 1339
Tru1I TTAA 6 cut(s) 672, 980, 990, 1008, 1305, 1496
Tru9I TTAA 6 cut(s) 672, 980, 990, 1008, 1305, 1496
TscAI CASTG 4 cut(s) 838, 964, 1371, 1399
TseFI GTSAC 1 cut(s) 953
TseI GCWGC 1 cut(s) 413
Tsp45I GTSAC 1 cut(s) 953
TspDTI ATGAA 6 cut(s) 477, 717, 1121, 1268, 1436, 1458
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 4 cut(s) 838, 964, 1371, 1399
Vha464I CTTAAG 1 cut(s) 671
VpaK11BI GGWCC 2 cut(s) 193, 1565
XapI RAATTY 4 cut(s) 88, 288, 622, 1231
XceI RCATGY 1 cut(s) 951
XmiI GTMKAC 1 cut(s) 1050
XspI CTAG 3 cut(s) 1025, 1409, 1431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.