RchiOBHm_Chr2g0159391

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009737
Physical Location & Seq
Reverse (-)
75542956 .. 75543983
1028 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGGGTCTGACAGTGACTCCGATGAGCCCAATGAGTACTACACTAGTGGCGAGGAAAGGTATATTCTTATTTGGTTCACAAAACTTTGGAGTAAAATTATTGGCTTTTTCCTTAGTAAGGATAATTTGCTGGTTTAGTAATGTGAATATAGAATCTAATGATTTGTGTTATGGTATGCTTGTCCAAGACCCCACCAGAGGCGGGGGTGACGTGGATTCAATTTTCACTCAAGCCAGACAGTTTGCACAAGGACCTTTGGAACCTTCGTCAAGCTCAAAAAGTTTTACTGGAACAGCTAGAACACTTACAGGAGAGACCGTTCCAACTGCTGCACCTCAGCCACCTGAGTCCATAAATCACACCATTACTTTATGGAGGAATGGCTTTTCTATAGATGACGGTCCGCTGAGGAGGCTGGATGATCCTGAAAATGCAGCTTTCTTAGAAGTAAGTTTTCATGAAATTATCCAATCGTTTCAATTATTTTCAGAGCTCTATACCTTTTTTTATTTTTTTAGCATACCAGCTTTTGTTTTAAGTGAAGTTTACTCTCTTCCATTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0000902 GO:0000904 GO:0002682 GO:0002683 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006520 GO:0006534 GO:0006555 GO:0006575 GO:0006725 GO:0006732 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009058 GO:0009066 GO:0009069 GO:0009116 GO:0009119 GO:0009653 GO:0009759 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010026 GO:0010090 GO:0010646 GO:0010648 GO:0016043 GO:0016143 GO:0016144 GO:0017144 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0023051 GO:0023057 GO:0030154 GO:0031323 GO:0031347 GO:0031348 GO:0032502 GO:0032989 GO:0033353 GO:0034641 GO:0042278 GO:0042343 GO:0042430 GO:0042435 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045088 GO:0045824 GO:0046128 GO:0046439 GO:0046483 GO:0046498 GO:0046500 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051186 GO:0055086 GO:0065007 GO:0071704 GO:0071840 GO:0072521 GO:0080134 GO:0080183 GO:0090342 GO:0090558 GO:0090626 GO:0097159 GO:1900457 GO:1900458 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901657 GO:1901659 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

188

Amino Acids

20.84

Weight (kDa)

4.79

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEP PF08059 122 - 149 2.8e-11 SEP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 201, 404
AclWI GGATC 1 cut(s) 416
AfaI GTAC 1 cut(s) 37
AfiI CCNNNNNNNGG 3 cut(s) 117, 197, 201
AgsI TTSAA 2 cut(s) 219, 479
AhlI ACTAGT 1 cut(s) 43
AjiI CACGTC 1 cut(s) 211
AloI GAACNNNNNNTCC 2 cut(s) 303, 335
AluBI AGCT 5 cut(s) 273, 296, 437, 493, 527
AluI AGCT 5 cut(s) 273, 296, 437, 493, 527
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 1 cut(s) 308
AlwI GGATC 1 cut(s) 416
ApeKI GCWGC 2 cut(s) 329, 434
AspS9I GGNCC 2 cut(s) 251, 401
AsuHPI GGTGA 1 cut(s) 218
AvaII GGWCC 2 cut(s) 251, 401
BanII GRGCYC 2 cut(s) 29, 495
Bbv12I GWGCWC 1 cut(s) 495
BbvCI CCTCAGC 2 cut(s) 336, 407
BbvI GCAGC 2 cut(s) 316, 446
BcoDI GTCTC 1 cut(s) 308
BcuI ACTAGT 1 cut(s) 43
BfaI CTAG 2 cut(s) 44, 297
BfmI CTRYAG 1 cut(s) 390
BisI GCNGC 2 cut(s) 330, 435
BlsI GCNGC 2 cut(s) 331, 436
BmcAI AGTACT 1 cut(s) 37
Bme18I GGWCC 2 cut(s) 251, 401
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 2 cut(s) 251, 401
BmiI GGNNCC 1 cut(s) 261
Bpu10I CCTNAGC 2 cut(s) 336, 407
BpuEI CTTGAG 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 308
BsaXI ACNNNNNCTCC 3 cut(s) 31, 303, 333
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 197, 201
Bse1I ACTGG 1 cut(s) 292
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 3 cut(s) 117, 197, 201
BseMII CTCAG 3 cut(s) 336, 350, 398
BseNI ACTGG 1 cut(s) 292
BseRI GAGGAG 1 cut(s) 424
BseXI GCAGC 2 cut(s) 316, 446
BsgI GTGCAG 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 495
BslI CCNNNNNNNGG 3 cut(s) 117, 197, 201
BsmAI GTCTC 1 cut(s) 308
Bso31I GGTCTC 1 cut(s) 308
Bsp1286I GDGCHC 2 cut(s) 29, 495
Bsp143I GATC 1 cut(s) 421
BspACI CCGC 2 cut(s) 201, 404
BspCNI CTCAG 3 cut(s) 337, 349, 399
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 1 cut(s) 261
BspPI GGATC 1 cut(s) 416
BspTNI GGTCTC 1 cut(s) 308
BsrI ACTGG 1 cut(s) 292
BssMI GATC 1 cut(s) 421
Bst4CI ACNGT 4 cut(s) 13, 240, 319, 401
Bst6I CTCTTC 1 cut(s) 558
BstDEI CTNAG 5 cut(s) 112, 336, 345, 407, 442
BstENI CCTNNNNNAGG 1 cut(s) 115
BstF5I GGATG 1 cut(s) 424
BstKTI GATC 1 cut(s) 424
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 1 cut(s) 421
BstMWI GCNNNNNNNGC 1 cut(s) 412
BstSFI CTRYAG 1 cut(s) 390
BstV1I GCAGC 2 cut(s) 316, 446
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 18
CciI TCATGA 1 cut(s) 457
Cfr13I GGNCC 2 cut(s) 251, 401
CpoI CGGWCCG 1 cut(s) 401
Csp6I GTAC 1 cut(s) 36
CspI CGGWCCG 1 cut(s) 401
CviAII CATG 1 cut(s) 458
CviQI GTAC 1 cut(s) 36
DdeI CTNAG 5 cut(s) 112, 336, 345, 407, 442
DpnI GATC 1 cut(s) 423
DpnII GATC 1 cut(s) 421
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
Ecl136II GAGCTC 1 cut(s) 493
Eco24I GRGCYC 2 cut(s) 29, 495
Eco31I GGTCTC 1 cut(s) 308
Eco47I GGWCC 2 cut(s) 251, 401
Eco53kI GAGCTC 1 cut(s) 493
EcoICRI GAGCTC 1 cut(s) 493
EcoNI CCTNNNNNAGG 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 251
EcoT38I GRGCYC 2 cut(s) 29, 495
FaeI CATG 1 cut(s) 461
FatI CATG 1 cut(s) 457
FauI CCCGC 1 cut(s) 194
Fnu4HI GCNGC 2 cut(s) 330, 435
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 2 cut(s) 29, 495
Fsp4HI GCNGC 2 cut(s) 330, 435
FspBI CTAG 2 cut(s) 44, 297
GluI GCNGC 2 cut(s) 330, 435
Hin1II CATG 1 cut(s) 461
HinfI GANTC 4 cut(s) 16, 152, 215, 347
HphI GGTGA 1 cut(s) 218
Hpy166II GTNNAC 2 cut(s) 77, 547
Hpy188I TCNGA 3 cut(s) 9, 21, 490
Hpy188III TCNNGA 2 cut(s) 425, 458
Hpy8I GTNNAC 2 cut(s) 77, 547
HpyAV CCTTC 1 cut(s) 273
HpyCH4III ACNGT 4 cut(s) 13, 240, 319, 401
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 3 cut(s) 245, 332, 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 412
HpyF3I CTNAG 5 cut(s) 112, 336, 345, 407, 442
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 1 cut(s) 461
Kzo9I GATC 1 cut(s) 421
LpnPI CCDG 9 cut(s) 115, 208, 247, 273, 294, 357, 401, 438, 537
Lsp1109I GCAGC 2 cut(s) 316, 446
MaeI CTAG 2 cut(s) 44, 297
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 2 cut(s) 13, 206
MalI GATC 1 cut(s) 423
MboI GATC 1 cut(s) 421
MboII GAAGA 1 cut(s) 545
MhlI GDGCHC 2 cut(s) 29, 495
MluCI AATT 5 cut(s) 95, 123, 219, 462, 479
MlyI GAGTC 2 cut(s) 10, 356
MmeI TCCRAC 1 cut(s) 347
MnlI CCTC 6 cut(s) 45, 191, 345, 369, 402, 405
MseI TTAA 1 cut(s) 536
MspA1I CMGCKG 1 cut(s) 406
MwoI GCNNNNNNNGC 1 cut(s) 412
NdeII GATC 1 cut(s) 421
NlaIII CATG 1 cut(s) 461
NlaIV GGNNCC 1 cut(s) 261
NmuCI GTSAC 2 cut(s) 13, 206
PagI TCATGA 1 cut(s) 457
PfeI GAWTC 2 cut(s) 152, 215
PkrI GCNGC 2 cut(s) 331, 436
PleI GAGTC 2 cut(s) 10, 355
PpsI GAGTC 2 cut(s) 10, 355
PpuMI RGGWCCY 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 495
Psp5II RGGWCCY 1 cut(s) 251
PspN4I GGNNCC 1 cut(s) 261
PspPI GGNCC 2 cut(s) 251, 401
PspPPI RGGWCCY 1 cut(s) 251
RsaI GTAC 1 cut(s) 37
RsaNI GTAC 1 cut(s) 36
Rsr2I CGGWCCG 1 cut(s) 401
RsrII CGGWCCG 1 cut(s) 401
SacI GAGCTC 1 cut(s) 495
SaqAI TTAA 1 cut(s) 536
SatI GCNGC 2 cut(s) 330, 435
Sau3AI GATC 1 cut(s) 421
Sau96I GGNCC 2 cut(s) 251, 401
ScaI AGTACT 1 cut(s) 37
SchI GAGTC 2 cut(s) 10, 356
SduI GDGCHC 2 cut(s) 29, 495
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 2 cut(s) 251, 401
SmlI CTYRAG 1 cut(s) 228
SmoI CTYRAG 1 cut(s) 228
SpeI ACTAGT 1 cut(s) 43
Sse9I AATT 5 cut(s) 95, 123, 219, 462, 479
SsiI CCGC 2 cut(s) 201, 404
SspMI CTAG 2 cut(s) 44, 297
SstI GAGCTC 1 cut(s) 495
TaaI ACNGT 4 cut(s) 13, 240, 319, 401
TaiI ACGT 1 cut(s) 213
TasI AATT 5 cut(s) 95, 123, 219, 462, 479
TatI WGTACW 1 cut(s) 35
TfiI GAWTC 2 cut(s) 152, 215
Tru1I TTAA 1 cut(s) 536
Tru9I TTAA 1 cut(s) 536
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 2 cut(s) 13, 206
TseI GCWGC 2 cut(s) 329, 434
Tsp45I GTSAC 2 cut(s) 13, 206
TspDTI ATGAA 2 cut(s) 446, 474
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 2 cut(s) 251, 401
XagI CCTNNNNNAGG 1 cut(s) 115
XspI CTAG 2 cut(s) 44, 297
ZrmI AGTACT 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.