Rh2BG555400

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009737
Physical Location & Seq
Reverse (-)
76484329 .. 76487185
2857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG555400.1

Sequence Viewer

Length: 642 bp
ATGCGGGTTTCCGACACTGCCCGAGACGTTATTCTCAGGTTGGATGGTGATTGCCTCACACATGGTGTCTTGCGCCTCCCATATCATCTTCTCGAACCGTGCTCAGAAGGAGGTCGGCGACGACGACTTGTGGATGAAAGACTCGAGCTTTTCCATCTCCGGCATCTCTTTCTCGATGACAGCGCATTGCGGAGTAAGGGAGGGGCAGCAGCAGCTGCTGCTCTCAAGGATGGGTCTGACAGTGACTCCGATGAGCCCAATGAGTACTACACTAGTGGCGAGAAAAGTGGTATGCTTGTCCAAGACCCCACTAGAGGCGGGGGTGACGTGGATTCAATTTTCACTCAAGCCAGACAGTTTGCACAAGGACCTTTGGAACCTTCGTCAAGCTCAAAAAGTTTTACTGGAACAACTAGAACACTTACAGGAGAGACCGTTCCAACTGCTGCACCTCAGCCACCTGAGTCCATCAATCACACCATTACTTTATGGAGGAATGGCTTTTCCGTAGATGACGGTCCGCTGCGGAGGCTGAATGATCTTGCAAATGCAGCTTTCTTTGAATTCAACATCACCATCAAAGATAAGCGGGCTACAGATAATTCTGATCTTCCTTTTGAATATTGTTTTACAGTCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0000902 GO:0000904 GO:0002682 GO:0002683 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006520 GO:0006534 GO:0006555 GO:0006575 GO:0006725 GO:0006732 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009058 GO:0009066 GO:0009069 GO:0009116 GO:0009119 GO:0009653 GO:0009759 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010026 GO:0010090 GO:0010646 GO:0010648 GO:0016043 GO:0016143 GO:0016144 GO:0017144 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0023051 GO:0023057 GO:0030154 GO:0031323 GO:0031347 GO:0031348 GO:0032502 GO:0032989 GO:0033353 GO:0034641 GO:0042278 GO:0042343 GO:0042430 GO:0042435 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045088 GO:0045824 GO:0046128 GO:0046439 GO:0046483 GO:0046498 GO:0046500 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051186 GO:0055086 GO:0065007 GO:0071704 GO:0071840 GO:0072521 GO:0080134 GO:0080183 GO:0090342 GO:0090558 GO:0090626 GO:0097159 GO:1900457 GO:1900458 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901657 GO:1901659 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

213

Amino Acids

23.49

Weight (kDa)

5.26

Isoelectric Point (pI)

49.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEP PF08059 161 - 188 7.6e-09 SEP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 4, 190, 318, 521, 526, 589
AcsI RAATTY 1 cut(s) 563
AdeI CACNNNGTG 1 cut(s) 65
AfaI GTAC 1 cut(s) 266
AfiI CCNNNNNNNGG 1 cut(s) 314
AgsI TTSAA 4 cut(s) 336, 563, 568, 620
AhlI ACTAGT 1 cut(s) 272
AjiI CACGTC 1 cut(s) 328
AloI GAACNNNNNNTCC 2 cut(s) 420, 452
AluBI AGCT 4 cut(s) 148, 215, 390, 554
AluI AGCT 4 cut(s) 148, 215, 390, 554
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 2 cut(s) 18, 425
AlwNI CAGNNNCTG 2 cut(s) 215, 218
Ama87I CYCGRG 2 cut(s) 21, 143
ApeKI GCWGC 8 cut(s) 206, 209, 212, 215, 218, 446, 523, 551
ApoI RAATTY 1 cut(s) 563
AspLEI GCGC 2 cut(s) 75, 185
AspS9I GGNCC 2 cut(s) 368, 518
AsuHPI GGTGA 3 cut(s) 59, 335, 565
AvaI CYCGRG 2 cut(s) 21, 143
AvaII GGWCC 2 cut(s) 368, 518
BanII GRGCYC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 104
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 8 cut(s) 202, 205, 218, 221, 224, 433, 510, 563
BccI CCATC 5 cut(s) 38, 162, 224, 476, 584
BcoDI GTCTC 2 cut(s) 18, 425
BcuI ACTAGT 1 cut(s) 272
BfaI CTAG 3 cut(s) 273, 312, 414
BfmI CTRYAG 1 cut(s) 594
BisI GCNGC 8 cut(s) 207, 210, 213, 216, 219, 447, 524, 552
BlsI GCNGC 8 cut(s) 208, 211, 214, 217, 220, 448, 525, 553
BmcAI AGTACT 1 cut(s) 266
Bme18I GGWCC 2 cut(s) 368, 518
BmeT110I CYCGRG 2 cut(s) 21, 143
BmgBI CACGTC 1 cut(s) 328
BmgT120I GGNCC 2 cut(s) 368, 518
BmiI GGNNCC 1 cut(s) 378
BmsI GCATC 1 cut(s) 172
Bpu10I CCTNAGC 1 cut(s) 453
BpuEI CTTGAG 2 cut(s) 209, 330
BsaI GGTCTC 1 cut(s) 425
BsaXI ACNNNNNCTCC 4 cut(s) 230, 260, 420, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse1I ACTGG 1 cut(s) 409
Bse3DI GCAATG 1 cut(s) 185
BseGI GGATG 3 cut(s) 49, 139, 235
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMI GCAATG 1 cut(s) 185
BseMII CTCAG 4 cut(s) 49, 117, 453, 467
BseNI ACTGG 1 cut(s) 409
BseXI GCAGC 8 cut(s) 202, 205, 218, 221, 224, 433, 510, 563
BsgI GTGCAG 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 104
BsiHKCI CYCGRG 2 cut(s) 21, 143
BsiSI CCGG 1 cut(s) 160
BslI CCNNNNNNNGG 1 cut(s) 314
BsmAI GTCTC 2 cut(s) 18, 425
BsmBI CGTCTC 1 cut(s) 18
Bso31I GGTCTC 1 cut(s) 425
BsoBI CYCGRG 2 cut(s) 21, 143
Bsp1286I GDGCHC 2 cut(s) 104, 258
Bsp143I GATC 2 cut(s) 538, 607
BspACI CCGC 6 cut(s) 4, 190, 318, 521, 526, 589
BspCNI CTCAG 4 cut(s) 48, 116, 454, 466
BspLI GGNNCC 1 cut(s) 378
BspTNI GGTCTC 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 185
BsrI ACTGG 1 cut(s) 409
BssMI GATC 2 cut(s) 538, 607
Bst4CI ACNGT 6 cut(s) 99, 242, 357, 436, 518, 634
BstAPI GCANNNNNTGC 2 cut(s) 215, 218
BstC8I GCNNGC 1 cut(s) 591
BstDEI CTNAG 4 cut(s) 35, 103, 453, 462
BstF5I GGATG 3 cut(s) 49, 139, 235
BstHHI GCGC 2 cut(s) 75, 185
BstKTI GATC 2 cut(s) 541, 610
BstMAI GTCTC 2 cut(s) 18, 425
BstMBI GATC 2 cut(s) 538, 607
BstMWI GCNNNNNNNGC 5 cut(s) 212, 215, 218, 529, 551
BstSFI CTRYAG 1 cut(s) 594
BstV1I GCAGC 8 cut(s) 202, 205, 218, 221, 224, 433, 510, 563
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 3 cut(s) 49, 139, 235
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 2 cut(s) 15, 247
Cac8I GCNNGC 1 cut(s) 591
CaiI CAGNNNCTG 2 cut(s) 215, 218
CfoI GCGC 2 cut(s) 75, 185
Cfr13I GGNCC 2 cut(s) 368, 518
CpoI CGGWCCG 1 cut(s) 518
Csp6I GTAC 1 cut(s) 265
CspI CGGWCCG 1 cut(s) 518
CviAII CATG 1 cut(s) 62
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 4 cut(s) 35, 103, 453, 462
DpnI GATC 2 cut(s) 540, 609
DpnII GATC 2 cut(s) 538, 607
DraIII CACNNNGTG 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 258
Eco31I GGTCTC 1 cut(s) 425
Eco47I GGWCC 2 cut(s) 368, 518
Eco88I CYCGRG 2 cut(s) 21, 143
EcoO109I RGGNCCY 1 cut(s) 368
EcoRI GAATTC 1 cut(s) 563
EcoT38I GRGCYC 1 cut(s) 258
Esp3I CGTCTC 1 cut(s) 18
FaeI CATG 1 cut(s) 65
FaiI YATR 4 cut(s) 63, 82, 293, 490
FatI CATG 1 cut(s) 61
FauI CCCGC 2 cut(s) 311, 582
Fnu4HI GCNGC 8 cut(s) 207, 210, 213, 216, 219, 447, 524, 552
FokI GGATG 3 cut(s) 56, 146, 242
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 8 cut(s) 207, 210, 213, 216, 219, 447, 524, 552
FspBI CTAG 3 cut(s) 273, 312, 414
GlaI GCGC 2 cut(s) 74, 184
GluI GCNGC 8 cut(s) 207, 210, 213, 216, 219, 447, 524, 552
HapII CCGG 1 cut(s) 160
HhaI GCGC 2 cut(s) 75, 185
Hin1II CATG 1 cut(s) 65
Hin6I GCGC 2 cut(s) 73, 183
HinP1I GCGC 2 cut(s) 73, 183
HinfI GANTC 4 cut(s) 141, 245, 332, 464
HpaII CCGG 1 cut(s) 160
HphI GGTGA 3 cut(s) 59, 335, 565
Hpy188I TCNGA 5 cut(s) 13, 106, 238, 250, 607
Hpy188III TCNNGA 2 cut(s) 92, 173
Hpy99I CGWCG 2 cut(s) 123, 126
HpyAV CCTTC 2 cut(s) 101, 390
HpyCH4III ACNGT 6 cut(s) 99, 242, 357, 436, 518, 634
HpyCH4IV ACGT 2 cut(s) 27, 327
HpyCH4V TGCA 4 cut(s) 362, 449, 545, 551
HpyF10VI GCNNNNNNNGC 5 cut(s) 212, 215, 218, 529, 551
HpyF3I CTNAG 4 cut(s) 35, 103, 453, 462
HpySE526I ACGT 2 cut(s) 27, 327
Hsp92II CATG 1 cut(s) 65
HspAI GCGC 2 cut(s) 73, 183
Kzo9I GATC 2 cut(s) 538, 607
LpnPI CCDG 7 cut(s) 22, 173, 364, 390, 411, 474, 622
Lsp1109I GCAGC 8 cut(s) 202, 205, 218, 221, 224, 433, 510, 563
LweI GCATC 1 cut(s) 172
MaeI CTAG 3 cut(s) 273, 312, 414
MaeII ACGT 2 cut(s) 27, 327
MaeIII GTNAC 2 cut(s) 242, 323
MalI GATC 2 cut(s) 540, 609
MboI GATC 2 cut(s) 538, 607
MboII GAAGA 2 cut(s) 80, 602
MhlI GDGCHC 2 cut(s) 104, 258
MluCI AATT 3 cut(s) 336, 563, 601
MlyI GAGTC 3 cut(s) 135, 239, 473
MmeI TCCRAC 3 cut(s) 21, 36, 464
MnlI CCTC 8 cut(s) 65, 86, 104, 194, 308, 462, 486, 522
MspA1I CMGCKG 2 cut(s) 215, 523
MspI CCGG 1 cut(s) 160
MwoI GCNNNNNNNGC 5 cut(s) 212, 215, 218, 529, 551
NdeII GATC 2 cut(s) 538, 607
NlaIII CATG 1 cut(s) 65
NlaIV GGNNCC 1 cut(s) 378
NmuCI GTSAC 2 cut(s) 242, 323
PaeR7I CTCGAG 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 121
PfeI GAWTC 1 cut(s) 332
PkrI GCNGC 8 cut(s) 208, 211, 214, 217, 220, 448, 525, 553
PleI GAGTC 3 cut(s) 135, 239, 472
PpsI GAGTC 3 cut(s) 135, 239, 472
PpuMI RGGWCCY 1 cut(s) 368
Psp5II RGGWCCY 1 cut(s) 368
PspN4I GGNNCC 1 cut(s) 378
PspPI GGNCC 2 cut(s) 368, 518
PspPPI RGGWCCY 1 cut(s) 368
PspXI VCTCGAGB 1 cut(s) 143
PstNI CAGNNNCTG 2 cut(s) 215, 218
PvuII CAGCTG 1 cut(s) 215
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
Rsr2I CGGWCCG 1 cut(s) 518
RsrII CGGWCCG 1 cut(s) 518
SatI GCNGC 8 cut(s) 207, 210, 213, 216, 219, 447, 524, 552
Sau3AI GATC 2 cut(s) 538, 607
Sau96I GGNCC 2 cut(s) 368, 518
ScaI AGTACT 1 cut(s) 266
SchI GAGTC 3 cut(s) 135, 239, 473
SduI GDGCHC 2 cut(s) 104, 258
SfaNI GCATC 1 cut(s) 172
SfcI CTRYAG 1 cut(s) 594
Sfr274I CTCGAG 1 cut(s) 143
SinI GGWCC 2 cut(s) 368, 518
SlaI CTCGAG 1 cut(s) 143
SmlI CTYRAG 3 cut(s) 143, 224, 345
SmoI CTYRAG 3 cut(s) 143, 224, 345
SpeI ACTAGT 1 cut(s) 272
Sse9I AATT 3 cut(s) 336, 563, 601
SsiI CCGC 6 cut(s) 4, 190, 318, 521, 526, 589
SspI AATATT 1 cut(s) 623
SspMI CTAG 3 cut(s) 273, 312, 414
TaaI ACNGT 6 cut(s) 99, 242, 357, 436, 518, 634
TaiI ACGT 2 cut(s) 30, 330
TaqI TCGA 3 cut(s) 93, 144, 174
TasI AATT 3 cut(s) 336, 563, 601
TatI WGTACW 1 cut(s) 264
TfiI GAWTC 1 cut(s) 332
TscAI CASTG 2 cut(s) 22, 247
TseFI GTSAC 2 cut(s) 242, 323
TseI GCWGC 8 cut(s) 206, 209, 212, 215, 218, 446, 523, 551
Tsp45I GTSAC 2 cut(s) 242, 323
TspDTI ATGAA 1 cut(s) 150
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 2 cut(s) 22, 247
VpaK11BI GGWCC 2 cut(s) 368, 518
XapI RAATTY 1 cut(s) 563
XhoI CTCGAG 1 cut(s) 143
XspI CTAG 3 cut(s) 273, 312, 414
ZrmI AGTACT 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.