RchiOBHm_Chr1g0347241

Choline kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002182
Physical Location & Seq
Forward (+)
39849343 .. 39850214
872 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 330 bp
ATGACTAACGAGGTGTTCGAAATAAAGTGGCCAACAAGCACCGGGGAAGTCTCGCGAAAGGTTGTGGTTAGGATTTACGGTGAGGGTGTTGAGGTGTTCTTTGACAGGGATAATGAGATCCGAACATTTGAATACATGTCAAAGAACGGTCAGGGACCTCGTCTGCTTGGCCGTTTTCCTAATGGCCGAGTTGAAGAGTTCATCCATGCACGGACACTTTCAGCATCTGATCTACTTGATCCAGATATATCTGCTCTTATAGCTACTAAAATGAAGGAATTCCATGATCTTGAGATGCCCGGTCCAAAGGATGTCGTCCTCTGGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko03010 ko05231 map00564 map01100 map03010 map05231
Pfam Domains
Protein Families
Choline_kin_N Choline_kinase

Protein Analysis

109

Amino Acids

12.46

Weight (kDa)

5.17

Isoelectric Point (pI)

28.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_kinase PF01633 1 - 106 7.3e-34 Choline/ethanolamine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 55
AclWI GGATC 2 cut(s) 112, 233
AcoI YGGCCR 3 cut(s) 29, 169, 184
AcsI RAATTY 1 cut(s) 278
AflIII ACRYGT 1 cut(s) 135
AgsI TTSAA 2 cut(s) 131, 194
AluBI AGCT 1 cut(s) 263
AluI AGCT 1 cut(s) 263
Alw26I GTCTC 1 cut(s) 55
AlwI GGATC 2 cut(s) 112, 233
AlwNI CAGNNNCTG 1 cut(s) 227
AoxI GGCC 3 cut(s) 29, 169, 184
ApoI RAATTY 1 cut(s) 278
Asp700I GAANNNNTTC 1 cut(s) 278
AspS9I GGNCC 2 cut(s) 155, 302
AsuC2I CCSGG 2 cut(s) 43, 300
AsuHPI GGTGA 1 cut(s) 92
AsuII TTCGAA 1 cut(s) 18
AvaII GGWCC 2 cut(s) 155, 302
BalI TGGCCA 1 cut(s) 31
BceAI ACGGC 1 cut(s) 156
BcnI CCSGG 2 cut(s) 43, 300
BcoDI GTCTC 1 cut(s) 55
Bme1390I CCNGG 2 cut(s) 43, 300
Bme18I GGWCC 2 cut(s) 155, 302
BmgT120I GGNCC 2 cut(s) 155, 302
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 2 cut(s) 43, 300
BmsI GCATC 2 cut(s) 233, 285
Bpu14I TTCGAA 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 2 cut(s) 43, 300
BsaJI CCNNGG 1 cut(s) 42
BseDI CCNNGG 1 cut(s) 42
BseGI GGATG 2 cut(s) 201, 316
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 3 cut(s) 31, 171, 186
BsiSI CCGG 2 cut(s) 42, 300
BslFI GGGAC 1 cut(s) 168
BsmAI GTCTC 1 cut(s) 55
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 3 cut(s) 31, 171, 186
Bsp119I TTCGAA 1 cut(s) 18
Bsp143I GATC 4 cut(s) 117, 229, 238, 286
Bsp68I TCGCGA 1 cut(s) 55
BspANI GGCC 3 cut(s) 31, 171, 186
BspFNI CGCG 1 cut(s) 55
BspLI GGNNCC 1 cut(s) 156
BspPI GGATC 2 cut(s) 112, 233
BspT104I TTCGAA 1 cut(s) 18
BssECI CCNNGG 1 cut(s) 42
BssMI GATC 4 cut(s) 117, 229, 238, 286
Bst4CI ACNGT 2 cut(s) 80, 149
Bst6I CTCTTC 1 cut(s) 189
BstBI TTCGAA 1 cut(s) 18
BstF5I GGATG 2 cut(s) 201, 316
BstFNI CGCG 1 cut(s) 55
BstKTI GATC 4 cut(s) 120, 232, 241, 289
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 4 cut(s) 117, 229, 238, 286
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 2 cut(s) 41, 298
BstUI CGCG 1 cut(s) 55
BstX2I RGATCY 1 cut(s) 117
BstYI RGATCY 1 cut(s) 117
BsuRI GGCC 3 cut(s) 31, 171, 186
BtsCI GGATG 2 cut(s) 201, 316
BtuMI TCGCGA 1 cut(s) 55
CaiI CAGNNNCTG 1 cut(s) 227
Cfr13I GGNCC 2 cut(s) 155, 302
CviAII CATG 3 cut(s) 136, 206, 284
CviJI RGCY 5 cut(s) 31, 171, 186, 263, 327
CviKI_1 RGCY 5 cut(s) 31, 171, 186, 263, 327
DpnI GATC 4 cut(s) 119, 231, 240, 288
DpnII GATC 4 cut(s) 117, 229, 238, 286
EaeI YGGCCR 3 cut(s) 29, 169, 184
Eam1104I CTCTTC 1 cut(s) 189
EarI CTCTTC 1 cut(s) 189
Eco47I GGWCC 2 cut(s) 155, 302
EcoO109I RGGNCCY 1 cut(s) 155
EcoRI GAATTC 1 cut(s) 278
FaeI CATG 3 cut(s) 139, 209, 287
FaiI YATR 5 cut(s) 137, 207, 248, 260, 285
FaqI GGGAC 1 cut(s) 168
FatI CATG 3 cut(s) 135, 205, 283
FokI GGATG 2 cut(s) 188, 323
HaeIII GGCC 3 cut(s) 31, 171, 186
HapII CCGG 2 cut(s) 42, 300
Hin1II CATG 3 cut(s) 139, 209, 287
HpaII CCGG 2 cut(s) 42, 300
HphI GGTGA 1 cut(s) 92
Hpy188I TCNGA 2 cut(s) 122, 229
Hpy188III TCNNGA 3 cut(s) 54, 242, 290
HpyAV CCTTC 1 cut(s) 268
HpyCH4III ACNGT 2 cut(s) 80, 149
HpyCH4V TGCA 1 cut(s) 209
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
Hsp92II CATG 3 cut(s) 139, 209, 287
Kzo9I GATC 4 cut(s) 117, 229, 238, 286
LpnPI CCDG 6 cut(s) 55, 91, 137, 255, 307, 313
LweI GCATC 2 cut(s) 233, 285
MalI GATC 4 cut(s) 119, 231, 240, 288
MboI GATC 4 cut(s) 117, 229, 238, 286
MboII GAAGA 1 cut(s) 206
MflI RGATCY 1 cut(s) 117
MlsI TGGCCA 1 cut(s) 31
MluCI AATT 1 cut(s) 278
MluNI TGGCCA 1 cut(s) 31
MnlI CCTC 5 cut(s) 4, 76, 85, 168, 329
Mox20I TGGCCA 1 cut(s) 31
MroXI GAANNNNTTC 1 cut(s) 278
MscI TGGCCA 1 cut(s) 31
Msp20I TGGCCA 1 cut(s) 31
MspI CCGG 2 cut(s) 42, 300
MspR9I CCNGG 2 cut(s) 43, 300
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 1 cut(s) 260
NciI CCSGG 2 cut(s) 43, 300
NdeII GATC 4 cut(s) 117, 229, 238, 286
NlaIII CATG 3 cut(s) 139, 209, 287
NlaIV GGNNCC 1 cut(s) 156
NmeAIII GCCGAG 1 cut(s) 212
NruI TCGCGA 1 cut(s) 55
NspI RCATGY 1 cut(s) 139
NspV TTCGAA 1 cut(s) 18
PciI ACATGT 1 cut(s) 135
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 278
PflFI GACNNNGTC 1 cut(s) 159
PpuMI RGGWCCY 1 cut(s) 155
PscI ACATGT 1 cut(s) 135
Psp5II RGGWCCY 1 cut(s) 155
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 2 cut(s) 155, 302
PspPPI RGGWCCY 1 cut(s) 155
PstNI CAGNNNCTG 1 cut(s) 227
PsuI RGATCY 1 cut(s) 117
PsyI GACNNNGTC 1 cut(s) 159
RruI TCGCGA 1 cut(s) 55
Sau3AI GATC 4 cut(s) 117, 229, 238, 286
Sau96I GGNCC 2 cut(s) 155, 302
ScrFI CCNGG 2 cut(s) 43, 300
SetI ASST 5 cut(s) 15, 63, 96, 160, 265
SfaNI GCATC 2 cut(s) 233, 285
SfuI TTCGAA 1 cut(s) 18
SinI GGWCC 2 cut(s) 155, 302
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 1 cut(s) 278
StyD4I CCNGG 2 cut(s) 41, 298
TaaI ACNGT 2 cut(s) 80, 149
TaqI TCGA 1 cut(s) 18
TasI AATT 1 cut(s) 278
TspDTI ATGAA 2 cut(s) 190, 287
TspGWI ACGGA 1 cut(s) 226
Tth111I GACNNNGTC 1 cut(s) 159
VpaK11BI GGWCC 2 cut(s) 155, 302
XapI RAATTY 1 cut(s) 278
XceI RCATGY 1 cut(s) 139
XmnI GAANNNNTTC 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.