RchiOBHm_Chr1g0359361

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0008440
Physical Location & Seq
Reverse (-)
51349731 .. 51351864
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
ATGTGTACGTATGCACTATATACCTTACCATTCTGGTTAGAGGTACTTCGAAACAAAATGGACATCTCAGATATCCTCTTTCTCCTTCTCTTCCTATTTCCTCATTTCTATGCACTAGAACTAGACGACTGTCCAGCCTCCATGTGTGGCAACACCGGCTTTCCTATCCGGTTCCCTTTCGGGCTACAAGGCTATCAACCCAAAAACTGTTCTTACCCAGGTTTCGACCTAACCTGCAATACACAGGGCATAACAGTCCTGAGACTTCCAAACTCTGGAGAATTTTTTGTCCGAGCAATCGATTATGTCACACAAGAGATTCAACTCTATGACCCTAGTAGTTGCCTTCCCAAAAGGCTTCTAAGCCTCAACCTTTCAGGCTCTCCTTTTGTTGCTGCTTTTTATCAGAACTACACCTTCCTCAGTTGCCCTGCTTCATTCACAGAGTCAAAGTTCACCCCCATTGATTGCCTCAGTAACTCCACAACCTCAGTCTTGGCAACCCCTTCCATCAGCCTCGCAAATTCAATGTCTAAGGTGTGCAAGATCATCGCTACAATGCCAGTTCCGGTTTCTGGGATAGTTGAGTCTGAGGATGGATTCTCAACCGACTTTGATTCAGACCTTAGTTTGACATGGTATGAGCCTGGTTGTACTCCTTGTGAAGGTGAAGGTGGCATTTGTGGCTTTGAGACCAACACTAGCCAAGTACTTGGTTGCTTCTACAATTCTAAAGGCAGATCAGGCGACGGTTTCCAAGTTTTCAGGATTATTTGTGTCTCCATCGCGGTACCAGCCCTCACAATGGCGATTGGGATCGCTTGCTTTGCATGCATCAAGGACAGGAGTGGGATCAGTCCTGGTCGCACTGCACAGCGGAACCCCACTGCAGTTGCAGCAGAACCCGAACAACAATCCACAATAGTAATGAATGGTCTGGATCAGACCACCATTGAATCCTATCAAAAGCTAGTTCTTGGTGAGAGCCGGCGGCTTCCAGGACCCAACGACACCACTTGCACTATATGCTTATCAGAGTATCTCACCAAAGACACAGTAAGATGCATCCCAGAGTGTAAACACTGCTTCCATGCTGAATGTGTTGATGAGTGGCTGCGATTGAACAGTACATGTCCAGTTTGTCGAAACTCCCCCTCCCCAGCTCACGTCAACTCAGATGGGGTCTGA

Protein Analysis

395

Amino Acids

43.28

Weight (kDa)

4.83

Isoelectric Point (pI)

57.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 44 - 111 2.2e-17 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 173 - 244 3e-08 Wall-associated receptor kinase C-terminal
zf-RING_2 PF13639 339 - 382 5.9e-13 Ring finger domain
zf-rbx1 PF12678 339 - 382 3.8e-06 RING-H2 zinc finger domain
zf-RING_11 PF17123 340 - 368 1.6e-06 RING-like zinc finger
zf-C3HC4_2 PF13923 340 - 381 6.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28040 AT2G25410 AT2G46493 AT2G46494 AT2G46495 AT2G46495 AT5G36001 AT5G36001
fragaria_vesca FvH4_7g16350
malus_domestica MD07G1148900.v1.1
prunus_persica Prupe.2G187400_v2.0.a1
pyrus_communis pycom07g14790
rosa_chinensis RchiOBHm_Chr1g0359361
rosa_laevigata RLG00000027876
rosa_multiflora Rmu_ssc0000358.1_g000002
rosa_roxburghii Rroxscaffold_4G00297050
rosa_rugosa Rorug01G0270500
rosa_samantha Rh1AG283900 Rh1AG284000 Rh1BG250100 Rh1CG268000 Rh1DG279000
rosa_wichuraiana Rw1G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 242
Acc65I GGTACC 1 cut(s) 790
AccB1I GGYRCC 1 cut(s) 790
AccB7I CCANNNNNTGG 1 cut(s) 275
AccII CGCG 1 cut(s) 788
AciI CCGC 3 cut(s) 788, 877, 991
AclWI GGATC 3 cut(s) 824, 860, 948
AcsI RAATTY 2 cut(s) 281, 523
AfaI GTAC 6 cut(s) 7, 45, 655, 711, 792, 1129
AfiI CCNNNNNNNGG 4 cut(s) 275, 575, 665, 805
AflIII ACRYGT 1 cut(s) 1130
AgsI TTSAA 4 cut(s) 323, 528, 956, 1123
AjiI CACGTC 1 cut(s) 1168
AjnI CCWGG 4 cut(s) 217, 646, 859, 997
AluBI AGCT 2 cut(s) 970, 1163
AluI AGCT 2 cut(s) 970, 1163
Alw26I GTCTC 3 cut(s) 256, 686, 784
AlwI GGATC 3 cut(s) 824, 860, 948
ApeKI GCWGC 3 cut(s) 395, 896, 1114
ApoI RAATTY 2 cut(s) 281, 523
ArsI GACNNNNNNTTYG 2 cut(s) 515, 547
Asp718I GGTACC 1 cut(s) 790
AspS9I GGNCC 1 cut(s) 1001
AsuHPI GGTGA 4 cut(s) 448, 680, 992, 1036
AsuII TTCGAA 1 cut(s) 49
AvaII GGWCC 1 cut(s) 1001
BanI GGYRCC 1 cut(s) 790
BbvI GCAGC 3 cut(s) 382, 908, 1101
BccI CCATC 4 cut(s) 518, 590, 791, 1172
BcgI CGANNNNNNTGC 2 cut(s) 532, 566
BciT130I CCWGG 4 cut(s) 219, 648, 861, 999
BcoDI GTCTC 3 cut(s) 256, 686, 784
BfaI CTAG 5 cut(s) 116, 122, 336, 702, 971
BfmI CTRYAG 1 cut(s) 888
BfuAI ACCTGC 1 cut(s) 242
BisI GCNGC 4 cut(s) 396, 897, 992, 1115
BlsI GCNGC 4 cut(s) 397, 898, 993, 1116
BmcAI AGTACT 1 cut(s) 711
Bme1390I CCNGG 4 cut(s) 219, 648, 861, 999
Bme18I GGWCC 1 cut(s) 1001
BmgBI CACGTC 1 cut(s) 1168
BmgT120I GGNCC 1 cut(s) 1001
BmiI GGNNCC 4 cut(s) 173, 792, 881, 1003
BmrFI CCNGG 4 cut(s) 219, 648, 861, 999
BmsI GCATC 3 cut(s) 843, 1052, 1074
BoxI GACNNNNGTC 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 49
Bsa29I ATCGAT 1 cut(s) 300
BsaAI YACGTR 1 cut(s) 9
BsaBI GATNNNNATC 1 cut(s) 815
BsaI GGTCTC 1 cut(s) 686
BsaJI CCNNGG 1 cut(s) 217
BsaWI WCCGGW 2 cut(s) 168, 568
BsaXI ACNNNNNCTCC 2 cut(s) 1139, 1169
Bsc4I CCNNNNNNNGG 4 cut(s) 275, 575, 665, 805
Bse118I RCCGGY 2 cut(s) 155, 987
Bse1I ACTGG 2 cut(s) 563, 1136
Bse8I GATNNNNATC 1 cut(s) 815
BseBI CCWGG 4 cut(s) 219, 648, 861, 999
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 2 cut(s) 601, 1065
BseJI GATNNNNATC 1 cut(s) 815
BseLI CCNNNNNNNGG 4 cut(s) 275, 575, 665, 805
BseMII CTCAG 7 cut(s) 81, 251, 436, 487, 504, 582, 1188
BseNI ACTGG 2 cut(s) 563, 1136
BseXI GCAGC 3 cut(s) 382, 908, 1101
BseYI CCCAGC 1 cut(s) 1159
BsgI GTGCAG 1 cut(s) 855
Bsh1236I CGCG 1 cut(s) 788
BshNI GGYRCC 1 cut(s) 790
BshVI ATCGAT 1 cut(s) 300
BsiSI CCGG 4 cut(s) 156, 169, 569, 988
BslI CCNNNNNNNGG 4 cut(s) 275, 575, 665, 805
BsmAI GTCTC 3 cut(s) 256, 686, 784
Bso31I GGTCTC 1 cut(s) 686
Bsp119I TTCGAA 1 cut(s) 49
Bsp143I GATC 5 cut(s) 546, 740, 816, 852, 940
BspACI CCGC 3 cut(s) 788, 877, 991
BspCNI CTCAG 7 cut(s) 80, 252, 435, 486, 503, 583, 1187
BspDI ATCGAT 1 cut(s) 300
BspFNI CGCG 1 cut(s) 788
BspLI GGNNCC 4 cut(s) 173, 792, 881, 1003
BspMAI CTGCAG 1 cut(s) 892
BspMI ACCTGC 1 cut(s) 242
BspPI GGATC 3 cut(s) 824, 860, 948
BspT104I TTCGAA 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 790
BspTNI GGTCTC 1 cut(s) 686
BsrFI RCCGGY 2 cut(s) 155, 987
BsrI ACTGG 2 cut(s) 563, 1136
BssAI RCCGGY 2 cut(s) 155, 987
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 5 cut(s) 546, 740, 816, 852, 940
Bst2UI CCWGG 4 cut(s) 219, 648, 861, 999
Bst4CI ACNGT 6 cut(s) 131, 209, 256, 752, 1057, 1127
Bst6I CTCTTC 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 1026
BstBAI YACGTR 1 cut(s) 9
BstBI TTCGAA 1 cut(s) 49
BstC8I GCNNGC 3 cut(s) 823, 832, 989
BstENI CCTNNNNNAGG 1 cut(s) 663
BstF5I GGATG 2 cut(s) 601, 1065
BstFNI CGCG 1 cut(s) 788
BstKTI GATC 5 cut(s) 549, 743, 819, 855, 943
BstMAI GTCTC 3 cut(s) 256, 686, 784
BstMBI GATC 5 cut(s) 546, 740, 816, 852, 940
BstMWI GCNNNNNNNGC 8 cut(s) 156, 684, 744, 794, 827, 831, 896, 1026
BstNI CCWGG 4 cut(s) 219, 648, 861, 999
BstNSI RCATGY 2 cut(s) 834, 1134
BstPAI GACNNNNGTC 1 cut(s) 129
BstSCI CCNGG 4 cut(s) 217, 646, 859, 997
BstSFI CTRYAG 1 cut(s) 888
BstSNI TACGTA 1 cut(s) 9
BstUI CGCG 1 cut(s) 788
BstV1I GCAGC 3 cut(s) 382, 908, 1101
BstXI CCANNNNNNTGG 1 cut(s) 713
Bsu15I ATCGAT 1 cut(s) 300
BsuTUI ATCGAT 1 cut(s) 300
BtgZI GCGATG 2 cut(s) 535, 769
BtrI CACGTC 1 cut(s) 1168
BtsCI GGATG 2 cut(s) 601, 1065
BtsI GCAGTG 3 cut(s) 867, 885, 1081
BtsIMutI CAGTG 3 cut(s) 867, 885, 1081
BveI ACCTGC 1 cut(s) 242
Cac8I GCNNGC 3 cut(s) 823, 832, 989
Cfr10I RCCGGY 2 cut(s) 155, 987
Cfr13I GGNCC 1 cut(s) 1001
ClaI ATCGAT 1 cut(s) 300
Csp6I GTAC 6 cut(s) 6, 44, 654, 710, 791, 1128
CspCI CAANNNNNGTGG 2 cut(s) 874, 909
CviAII CATG 5 cut(s) 142, 636, 831, 1091, 1131
CviQI GTAC 6 cut(s) 6, 44, 654, 710, 791, 1128
DpnI GATC 5 cut(s) 548, 742, 818, 854, 942
DpnII GATC 5 cut(s) 546, 740, 816, 852, 940
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
Eco105I TACGTA 1 cut(s) 9
Eco31I GGTCTC 1 cut(s) 686
Eco32I GATATC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 1001
EcoNI CCTNNNNNAGG 1 cut(s) 663
EcoO109I RGGNCCY 1 cut(s) 1001
EcoRII CCWGG 4 cut(s) 217, 646, 859, 997
EcoRV GATATC 1 cut(s) 73
EcoT22I ATGCAT 2 cut(s) 836, 1067
FaeI CATG 5 cut(s) 145, 639, 834, 1094, 1134
FatI CATG 5 cut(s) 141, 635, 830, 1090, 1130
Fnu4HI GCNGC 4 cut(s) 396, 897, 992, 1115
FokI GGATG 2 cut(s) 608, 1052
Fsp4HI GCNGC 4 cut(s) 396, 897, 992, 1115
FspBI CTAG 5 cut(s) 116, 122, 336, 702, 971
GluI GCNGC 4 cut(s) 396, 897, 992, 1115
GsaI CCCAGC 1 cut(s) 1163
GsuI CTGGAG 1 cut(s) 297
HapII CCGG 4 cut(s) 156, 169, 569, 988
Hin1II CATG 5 cut(s) 145, 639, 834, 1094, 1134
HincII GTYRAC 1 cut(s) 1171
HindII GTYRAC 1 cut(s) 1171
HinfI GANTC 6 cut(s) 319, 446, 587, 600, 617, 956
HpaII CCGG 4 cut(s) 156, 169, 569, 988
HphI GGTGA 4 cut(s) 448, 680, 992, 1036
Hpy166II GTNNAC 4 cut(s) 6, 456, 1079, 1171
Hpy188I TCNGA 9 cut(s) 70, 293, 408, 592, 622, 945, 1036, 1177, 1187
Hpy188III TCNNGA 4 cut(s) 259, 276, 766, 938
Hpy8I GTNNAC 4 cut(s) 6, 456, 1079, 1171
Hpy99I CGWCG 1 cut(s) 752
HpyAV CCTTC 6 cut(s) 95, 356, 427, 516, 659, 665
HpyCH4III ACNGT 6 cut(s) 131, 209, 256, 752, 1057, 1127
HpyCH4IV ACGT 2 cut(s) 8, 1167
HpyF10VI GCNNNNNNNGC 8 cut(s) 156, 684, 744, 794, 827, 831, 896, 1026
HpySE526I ACGT 2 cut(s) 8, 1167
Hsp92II CATG 5 cut(s) 145, 639, 834, 1094, 1134
KpnI GGTACC 1 cut(s) 794
KroI GCCGGC 1 cut(s) 987
KroNI GCCGGC 1 cut(s) 989
Kzo9I GATC 5 cut(s) 546, 740, 816, 852, 940
Lsp1109I GCAGC 3 cut(s) 382, 908, 1101
LweI GCATC 3 cut(s) 843, 1052, 1074
MaeI CTAG 5 cut(s) 116, 122, 336, 702, 971
MaeII ACGT 2 cut(s) 8, 1167
MaeIII GTNAC 2 cut(s) 307, 476
MalI GATC 5 cut(s) 548, 742, 818, 854, 942
MboI GATC 5 cut(s) 546, 740, 816, 852, 940
MboII GAAGA 1 cut(s) 82
MluCI AATT 3 cut(s) 281, 523, 727
MlyI GAGTC 2 cut(s) 455, 596
Mph1103I ATGCAT 2 cut(s) 836, 1067
MroNI GCCGGC 1 cut(s) 987
MslI CAYNNNNRTG 1 cut(s) 108
MspA1I CMGCKG 1 cut(s) 877
MspI CCGG 4 cut(s) 156, 169, 569, 988
MspR9I CCNGG 4 cut(s) 219, 648, 861, 999
MvaI CCWGG 4 cut(s) 219, 648, 861, 999
MvnI CGCG 1 cut(s) 788
MwoI GCNNNNNNNGC 8 cut(s) 156, 684, 744, 794, 827, 831, 896, 1026
NaeI GCCGGC 1 cut(s) 989
NdeII GATC 5 cut(s) 546, 740, 816, 852, 940
NgoMIV GCCGGC 1 cut(s) 987
NlaIII CATG 5 cut(s) 145, 639, 834, 1094, 1134
NlaIV GGNNCC 4 cut(s) 173, 792, 881, 1003
NmuCI GTSAC 1 cut(s) 307
NsiI ATGCAT 2 cut(s) 836, 1067
NspI RCATGY 2 cut(s) 834, 1134
NspV TTCGAA 1 cut(s) 49
PaeI GCATGC 1 cut(s) 834
PciI ACATGT 1 cut(s) 1130
PdiI GCCGGC 1 cut(s) 989
PfeI GAWTC 4 cut(s) 319, 600, 617, 956
PflMI CCANNNNNTGG 1 cut(s) 275
PfoI TCCNGGA 1 cut(s) 997
PkrI GCNGC 4 cut(s) 397, 898, 993, 1116
PleI GAGTC 2 cut(s) 454, 595
PpsI GAGTC 2 cut(s) 454, 595
Ppu21I YACGTR 1 cut(s) 9
PpuMI RGGWCCY 1 cut(s) 1001
PscI ACATGT 1 cut(s) 1130
PshAI GACNNNNGTC 1 cut(s) 129
Psp5II RGGWCCY 1 cut(s) 1001
Psp6I CCWGG 4 cut(s) 217, 646, 859, 997
PspFI CCCAGC 1 cut(s) 1159
PspGI CCWGG 4 cut(s) 217, 646, 859, 997
PspN4I GGNNCC 4 cut(s) 173, 792, 881, 1003
PspPI GGNCC 1 cut(s) 1001
PspPPI RGGWCCY 1 cut(s) 1001
PstI CTGCAG 1 cut(s) 892
RsaI GTAC 6 cut(s) 7, 45, 655, 711, 792, 1129
RsaNI GTAC 6 cut(s) 6, 44, 654, 710, 791, 1128
RseI CAYNNNNRTG 1 cut(s) 108
SatI GCNGC 4 cut(s) 396, 897, 992, 1115
Sau3AI GATC 5 cut(s) 546, 740, 816, 852, 940
Sau96I GGNCC 1 cut(s) 1001
ScaI AGTACT 1 cut(s) 711
SchI GAGTC 2 cut(s) 455, 596
ScrFI CCNGG 4 cut(s) 219, 648, 861, 999
SfaNI GCATC 3 cut(s) 843, 1052, 1074
SfcI CTRYAG 1 cut(s) 888
SfuI TTCGAA 1 cut(s) 49
SinI GGWCC 1 cut(s) 1001
SmiMI CAYNNNNRTG 1 cut(s) 108
SnaBI TACGTA 1 cut(s) 9
SphI GCATGC 1 cut(s) 834
Sse9I AATT 3 cut(s) 281, 523, 727
SsiI CCGC 3 cut(s) 788, 877, 991
SspMI CTAG 5 cut(s) 116, 122, 336, 702, 971
StyD4I CCNGG 4 cut(s) 217, 646, 859, 997
TaaI ACNGT 6 cut(s) 131, 209, 256, 752, 1057, 1127
TaiI ACGT 2 cut(s) 11, 1170
TaqI TCGA 4 cut(s) 49, 225, 300, 1144
TasI AATT 3 cut(s) 281, 523, 727
TatI WGTACW 3 cut(s) 653, 709, 1127
TauI GCSGC 1 cut(s) 994
TfiI GAWTC 4 cut(s) 319, 600, 617, 956
TscAI CASTG 3 cut(s) 874, 892, 1088
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 3 cut(s) 395, 896, 1114
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 2 cut(s) 426, 944
TspRI CASTG 3 cut(s) 874, 892, 1088
Van91I CCANNNNNTGG 1 cut(s) 275
VpaK11BI GGWCC 1 cut(s) 1001
XagI CCTNNNNNAGG 1 cut(s) 663
XapI RAATTY 2 cut(s) 281, 523
XceI RCATGY 2 cut(s) 834, 1134
XspI CTAG 5 cut(s) 116, 122, 336, 702, 971
ZrmI AGTACT 1 cut(s) 711
Zsp2I ATGCAT 2 cut(s) 836, 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.