RchiOBHm_Chr1g0377401

Actin depolymerisation factor/cofilin -like domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0008202
Physical Location & Seq
Reverse (-)
64369354 .. 64371753
2400 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 846 bp
ATGGCGAACAAAAGACAGAGAGAGGCACGCAAGAGATTCAGAGCAGAGCACCCAGAACTGGTTAAAACCCCAGAGCCAACTCCCCCGAAAGACCCAAGCAAGAAGAAGAAGGTAAAGAAGCCCAGTTTCAAGCGCAAGAGATCAGAATCTGGAGCGCCCAATGACCCCAATAGAGCAGCCAAGAAGTCTCGGAGTAAACACCCACTTAGAGTCCCTGGTATGAAGCCCGGAGATAGCTGCTTCATTTGTAAAGGCACTGACCATATTGCCAAGCTTTGCCCTGAGAAAGCTGAGTGGGATAGGCACAAGATATGCTTGTTTTGTCGACAACGCGGGCATAGTCTGAAGAATTGCCAAAAGAAGAATGAGAATGATACGGAGGCGAAGTTATGCTATAATTGTGGGGAAACCGGGCATTCGCTCTCTAGATGCCCCTTATCTCTTCAAGATGGAGGAACTAAATTTGCCAAGTGCTTCGTCTGTAACGAGACTGGTCACCTGAGCAAGGACTGTCCTAAAAACTCTCATGGGATTTATCCCAAGGGTGGTTCTTGTAAAATATGTGGTGGTGTGACACATTTGGCACGAGATTGTCCTAACAAAGACAGCAGGAATTCAATAGGTTCTGGCAATTCTAATCCCAAATGGAATGAACCAAGAGGAAAGGTTACCAAATTTGCGAGTGGAGATGATCTCGAGGATGATTTCAGTTTCATAGGTGAGAAGACTGTTGCCAAAACAGTAGACGAGTCTGCCAATGTAGTTGGTACTGCTTCTCTTCAACAAGAAAGTAACGTAAAATCAAAGAAGAAACAGGGTCCCAAAATAGTAAATTTTGCCGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF zf-CCHC zf-CCHC_4

Protein Analysis

281

Amino Acids

31.03

Weight (kDa)

9.66

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 157 - 173 3e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 325, 744
AccII CGCG 1 cut(s) 333
AciI CCGC 1 cut(s) 333
AcsI RAATTY 4 cut(s) 461, 613, 674, 832
AcuI CTGAAG 1 cut(s) 365
AfaI GTAC 1 cut(s) 769
AfiI CCNNNNNNNGG 3 cut(s) 58, 505, 545
AgsI TTSAA 4 cut(s) 130, 446, 618, 782
AjnI CCWGG 1 cut(s) 214
AluBI AGCT 3 cut(s) 237, 274, 290
AluI AGCT 3 cut(s) 237, 274, 290
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 2 cut(s) 192, 482
AlwNI CAGNNNCTG 1 cut(s) 149
Ama87I CYCGRG 1 cut(s) 695
ApeKI GCWGC 2 cut(s) 176, 237
ApoI RAATTY 4 cut(s) 461, 613, 674, 832
AspLEI GCGC 2 cut(s) 135, 157
AspS9I GGNCC 1 cut(s) 818
AsuC2I CCSGG 2 cut(s) 228, 412
AsuHPI GGTGA 2 cut(s) 488, 731
AvaI CYCGRG 1 cut(s) 695
AvaII GGWCC 1 cut(s) 818
BauI CACGAG 1 cut(s) 585
BbsI GAAGAC 1 cut(s) 731
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 2 cut(s) 188, 224
BccI CCATC 1 cut(s) 443
BciT130I CCWGG 1 cut(s) 216
BcnI CCSGG 2 cut(s) 228, 412
BcoDI GTCTC 2 cut(s) 192, 482
BfaI CTAG 1 cut(s) 426
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 2 cut(s) 177, 238
BlsI GCNGC 2 cut(s) 178, 239
Bme1390I CCNGG 3 cut(s) 216, 228, 412
Bme18I GGWCC 1 cut(s) 818
BmeT110I CYCGRG 1 cut(s) 695
BmgT120I GGNCC 1 cut(s) 818
BmiI GGNNCC 2 cut(s) 819, 820
BmrFI CCNGG 3 cut(s) 216, 228, 412
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 419
BmuI ACTGGG 1 cut(s) 117
BpiI GAAGAC 1 cut(s) 731
BplI GAGNNNNNCTC 2 cut(s) 678, 710
BpmI CTGGAG 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 500
BpuMI CCSGG 2 cut(s) 228, 412
BsaBI GATNNNNATC 1 cut(s) 145
BsaJI CCNNGG 2 cut(s) 214, 540
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 505, 545
Bse1I ACTGG 3 cut(s) 63, 123, 496
Bse8I GATNNNNATC 1 cut(s) 145
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 2 cut(s) 214, 540
BseGI GGATG 1 cut(s) 706
BseJI GATNNNNATC 1 cut(s) 145
BseLI CCNNNNNNNGG 3 cut(s) 58, 505, 545
BseMII CTCAG 3 cut(s) 273, 282, 491
BseNI ACTGG 3 cut(s) 63, 123, 496
BseXI GCAGC 2 cut(s) 188, 224
Bsh1236I CGCG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 695
BsiSI CCGG 3 cut(s) 228, 411, 840
BslFI GGGAC 2 cut(s) 197, 804
BslI CCNNNNNNNGG 3 cut(s) 58, 505, 545
BsmAI GTCTC 2 cut(s) 192, 482
BsmFI GGGAC 2 cut(s) 197, 804
BsmI GAATGC 1 cut(s) 415
BsoBI CYCGRG 1 cut(s) 695
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 2 cut(s) 140, 691
BspACI CCGC 1 cut(s) 333
BspCNI CTCAG 3 cut(s) 274, 283, 492
BspFNI CGCG 1 cut(s) 333
BspLI GGNNCC 2 cut(s) 819, 820
BsrI ACTGG 3 cut(s) 63, 123, 496
BssECI CCNNGG 2 cut(s) 214, 540
BssMI GATC 2 cut(s) 140, 691
BssSI CACGAG 1 cut(s) 585
BssT1I CCWWGG 1 cut(s) 540
Bst2BI CACGAG 1 cut(s) 585
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 3 cut(s) 512, 730, 742
Bst6I CTCTTC 2 cut(s) 447, 783
BstC8I GCNNGC 2 cut(s) 28, 335
BstDEI CTNAG 4 cut(s) 206, 282, 291, 500
BstEII GGTNACC 2 cut(s) 494, 667
BstENI CCTNNNNNAGG 1 cut(s) 503
BstF5I GGATG 1 cut(s) 706
BstFNI CGCG 1 cut(s) 333
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 2 cut(s) 135, 157
BstKTI GATC 2 cut(s) 143, 694
BstMAI GTCTC 2 cut(s) 192, 482
BstMBI GATC 2 cut(s) 140, 691
BstNI CCWGG 1 cut(s) 216
BstPI GGTNACC 2 cut(s) 494, 667
BstSCI CCNGG 3 cut(s) 214, 226, 410
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 188, 224
BstV2I GAAGAC 1 cut(s) 731
BtsCI GGATG 1 cut(s) 706
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 28, 335
CaiI CAGNNNCTG 1 cut(s) 149
CfoI GCGC 2 cut(s) 135, 157
Cfr13I GGNCC 1 cut(s) 818
Csp6I GTAC 1 cut(s) 768
CviAII CATG 1 cut(s) 527
CviJI RGCY 7 cut(s) 76, 121, 179, 226, 237, 274, 290
CviKI_1 RGCY 7 cut(s) 76, 121, 179, 226, 237, 274, 290
CviQI GTAC 1 cut(s) 768
DdeI CTNAG 4 cut(s) 206, 282, 291, 500
DpnI GATC 2 cut(s) 142, 693
DpnII GATC 2 cut(s) 140, 691
Eam1104I CTCTTC 2 cut(s) 447, 783
EarI CTCTTC 2 cut(s) 447, 783
Eco130I CCWWGG 1 cut(s) 540
Eco47I GGWCC 1 cut(s) 818
Eco57I CTGAAG 1 cut(s) 365
Eco88I CYCGRG 1 cut(s) 695
Eco91I GGTNACC 2 cut(s) 494, 667
EcoNI CCTNNNNNAGG 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 818
EcoO65I GGTNACC 2 cut(s) 494, 667
EcoRI GAATTC 1 cut(s) 613
EcoRII CCWGG 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 540
ErhI CCWWGG 1 cut(s) 540
FaeI CATG 1 cut(s) 530
FaiI YATR 9 cut(s) 221, 264, 313, 339, 391, 396, 528, 562, 716
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 2 cut(s) 197, 804
FatI CATG 1 cut(s) 526
FauI CCCGC 1 cut(s) 326
FblI GTMKAC 2 cut(s) 325, 744
Fnu4HI GCNGC 2 cut(s) 177, 238
FokI GGATG 1 cut(s) 713
Fsp4HI GCNGC 2 cut(s) 177, 238
FspBI CTAG 1 cut(s) 426
GlaI GCGC 2 cut(s) 134, 156
GluI GCNGC 2 cut(s) 177, 238
GsuI CTGGAG 1 cut(s) 171
HaeII RGCGCY 1 cut(s) 158
HapII CCGG 3 cut(s) 228, 411, 840
HhaI GCGC 2 cut(s) 135, 157
Hin1II CATG 1 cut(s) 530
Hin6I GCGC 2 cut(s) 133, 155
HinP1I GCGC 2 cut(s) 133, 155
HincII GTYRAC 1 cut(s) 326
HindII GTYRAC 1 cut(s) 326
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 4 cut(s) 36, 146, 210, 749
HpaII CCGG 3 cut(s) 228, 411, 840
HphI GGTGA 2 cut(s) 488, 731
Hpy166II GTNNAC 3 cut(s) 197, 326, 745
Hpy188I TCNGA 4 cut(s) 41, 145, 192, 345
Hpy188III TCNNGA 4 cut(s) 150, 426, 446, 695
Hpy8I GTNNAC 3 cut(s) 197, 326, 745
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 3 cut(s) 512, 730, 742
HpyCH4IV ACGT 1 cut(s) 795
HpyF3I CTNAG 4 cut(s) 206, 282, 291, 500
HpySE526I ACGT 1 cut(s) 795
Hsp92II CATG 1 cut(s) 530
HspAI GCGC 2 cut(s) 133, 155
KflI GGGWCCC 1 cut(s) 818
Kzo9I GATC 2 cut(s) 140, 691
LmnI GCTCC 1 cut(s) 152
Lsp1109I GCAGC 2 cut(s) 188, 224
LweI GCATC 1 cut(s) 419
MaeI CTAG 1 cut(s) 426
MaeII ACGT 1 cut(s) 795
MaeIII GTNAC 5 cut(s) 482, 494, 571, 667, 791
MalI GATC 2 cut(s) 142, 693
MboI GATC 2 cut(s) 140, 691
MboII GAAGA 8 cut(s) 115, 118, 358, 373, 434, 736, 770, 820
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 7 cut(s) 349, 397, 461, 613, 631, 674, 832
MlyI GAGTC 2 cut(s) 219, 758
MnlI CCTC 5 cut(s) 16, 373, 446, 653, 691
MseI TTAA 1 cut(s) 63
MspI CCGG 3 cut(s) 228, 411, 840
MspR9I CCNGG 3 cut(s) 216, 228, 412
Mva1269I GAATGC 1 cut(s) 415
MvaI CCWGG 1 cut(s) 216
MvnI CGCG 1 cut(s) 333
NciI CCSGG 2 cut(s) 228, 412
NdeII GATC 2 cut(s) 140, 691
NlaIII CATG 1 cut(s) 530
NlaIV GGNNCC 2 cut(s) 819, 820
NmuCI GTSAC 2 cut(s) 494, 571
PaeR7I CTCGAG 1 cut(s) 695
PcsI WCGNNNNNNNCGW 1 cut(s) 483
PctI GAATGC 1 cut(s) 415
PfeI GAWTC 2 cut(s) 36, 146
PkrI GCNGC 2 cut(s) 178, 239
PleI GAGTC 2 cut(s) 218, 757
PpsI GAGTC 2 cut(s) 218, 757
PpuMI RGGWCCY 1 cut(s) 818
Psp5II RGGWCCY 1 cut(s) 818
Psp6I CCWGG 1 cut(s) 214
PspEI GGTNACC 2 cut(s) 494, 667
PspGI CCWGG 1 cut(s) 214
PspN4I GGNNCC 2 cut(s) 819, 820
PspPI GGNCC 1 cut(s) 818
PspPPI RGGWCCY 1 cut(s) 818
PstNI CAGNNNCTG 1 cut(s) 149
RsaI GTAC 1 cut(s) 769
RsaNI GTAC 1 cut(s) 768
SalI GTCGAC 1 cut(s) 324
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 2 cut(s) 177, 238
Sau3AI GATC 2 cut(s) 140, 691
Sau96I GGNCC 1 cut(s) 818
SchI GAGTC 2 cut(s) 219, 758
ScrFI CCNGG 3 cut(s) 216, 228, 412
SduI GDGCHC 1 cut(s) 51
SetI ASST 9 cut(s) 114, 239, 276, 292, 501, 625, 669, 721, 798
SfaNI GCATC 1 cut(s) 419
Sfr274I CTCGAG 1 cut(s) 695
SinI GGWCC 1 cut(s) 818
SlaI CTCGAG 1 cut(s) 695
SmlI CTYRAG 1 cut(s) 695
SmoI CTYRAG 1 cut(s) 695
Sse9I AATT 7 cut(s) 349, 397, 461, 613, 631, 674, 832
SsiI CCGC 1 cut(s) 333
SspMI CTAG 1 cut(s) 426
StyD4I CCNGG 3 cut(s) 214, 226, 410
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 3 cut(s) 512, 730, 742
TaiI ACGT 1 cut(s) 798
TaqI TCGA 2 cut(s) 325, 696
TasI AATT 7 cut(s) 349, 397, 461, 613, 631, 674, 832
TfiI GAWTC 2 cut(s) 36, 146
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 2 cut(s) 494, 571
TseI GCWGC 2 cut(s) 176, 237
Tsp45I GTSAC 2 cut(s) 494, 571
TspDTI ATGAA 4 cut(s) 232, 236, 666, 703
TspGWI ACGGA 1 cut(s) 392
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 818
XagI CCTNNNNNAGG 1 cut(s) 503
XapI RAATTY 4 cut(s) 461, 613, 674, 832
XbaI TCTAGA 1 cut(s) 425
XhoI CTCGAG 1 cut(s) 695
XmiI GTMKAC 2 cut(s) 325, 744
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.