RchiOBHm_Chr1g0347631

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004826
Physical Location & Seq
Forward (+)
40332410 .. 40333327
918 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57373

Sequence Viewer

Length: 642 bp
ATGTACGGACAGACAACCCTAGAGTCAGTAGATTCCGACTTGGCTCTTCTTGAATCCATTCGACAACACCTTCTAGGCGATCATATGGAGCCTCCGGCAACCATCTTCGGCGGCGACGTAGACGACATCATCCCCTTGTTTTTCCCCAGTTCTACTGCTGCTTCAACCTGCTTAAGTGGGCTTTTTACCACGGAGAACTGGTGGGAATCGGCGCTAAAACCCGAGGACTCTGAATCCGAAGCTGTAAGTACACCGGAGGAGGAGGAGGTTAAGTTGAGGAAGCAAGCTCAGAATGCGTCGGTTAAACGTGATGTGCACGCACCCAGGGTGGAGAGGCATTTCAGGGGCGTGAGGAGGAGGCCTTGGGGGAAGTACGCGGCGGAGATTAGAGATCCGGCGAAGAATGGCGCGAGAGTGTGGCTCGGGACTTACGAGACTCCCGAGGATGCTGGTCTGGCTTACGACCGAGCTGCTTTCAAACTGCGCGGCTGTAAAGCCAAACTCAACTTTCCTCACTTGATTGGCTCGAACAGCGTTGAGCCGGTTAGGGTTACCCCGAAGCGGCGGCGCTCGCCGGAACTGTCCTCTACGTCCTTGTCGGCATCGGAGGACAACGAGTCTCCCAAGGCAAAGCGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

213

Amino Acids

23.78

Weight (kDa)

6.54

Isoelectric Point (pI)

66.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 113 - 163 4.3e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 176
AccI GTMKAC 1 cut(s) 120
AccII CGCG 3 cut(s) 377, 410, 486
AciI CCGC 6 cut(s) 111, 377, 380, 486, 562, 565
AclWI GGATC 1 cut(s) 386
AfaI GTAC 3 cut(s) 5, 250, 374
AfiI CCNNNNNNNGG 1 cut(s) 561
AflII CTTAAG 1 cut(s) 172
AgsI TTSAA 3 cut(s) 53, 165, 478
AhdI GACNNNNNGTC 1 cut(s) 616
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 3 cut(s) 242, 287, 470
AluI AGCT 3 cut(s) 242, 287, 470
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 2 cut(s) 428, 624
Alw44I GTGCAC 1 cut(s) 314
AlwI GGATC 1 cut(s) 386
Ama87I CYCGRG 3 cut(s) 221, 422, 440
AoxI GGCC 1 cut(s) 359
ApaLI GTGCAC 1 cut(s) 314
ApeKI GCWGC 2 cut(s) 158, 470
Asp700I GAANNNNTTC 1 cut(s) 57
AspLEI GCGC 4 cut(s) 214, 410, 486, 570
AvaI CYCGRG 3 cut(s) 221, 422, 440
BaeGI GKGCMC 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 318
BbvI GCAGC 2 cut(s) 145, 457
BccI CCATC 1 cut(s) 110
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 1 cut(s) 325
BcoDI GTCTC 2 cut(s) 428, 624
BfaI CTAG 2 cut(s) 20, 74
BfoI RGCGCY 2 cut(s) 215, 571
BfrI CTTAAG 1 cut(s) 172
BfuAI ACCTGC 1 cut(s) 176
BisI GCNGC 7 cut(s) 112, 159, 378, 471, 487, 563, 566
BlsI GCNGC 7 cut(s) 113, 160, 379, 472, 488, 564, 567
Bme1390I CCNGG 1 cut(s) 325
BmeRI GACNNNNNGTC 1 cut(s) 616
BmeT110I CYCGRG 3 cut(s) 221, 422, 440
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 325
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 2 cut(s) 436, 611
BmuI ACTGGG 1 cut(s) 141
BplI GAGNNNNNCTC 2 cut(s) 405, 437
BsaJI CCNNGG 7 cut(s) 189, 222, 323, 324, 362, 441, 624
BsaWI WCCGGW 1 cut(s) 253
BsaXI ACNNNNNCTCC 4 cut(s) 185, 215, 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 561
Bse118I RCCGGY 1 cut(s) 541
Bse1I ACTGG 2 cut(s) 147, 203
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 7 cut(s) 189, 222, 323, 324, 362, 441, 624
BseGI GGATG 2 cut(s) 129, 451
BseLI CCNNNNNNNGG 1 cut(s) 561
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 2 cut(s) 147, 203
BseRI GAGGAG 5 cut(s) 272, 275, 278, 367, 370
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 2 cut(s) 145, 457
Bsh1236I CGCG 3 cut(s) 377, 410, 486
Bsh1285I CGRYCG 1 cut(s) 466
BshFI GGCC 1 cut(s) 361
BsiEI CGRYCG 1 cut(s) 466
BsiHKAI GWGCWC 1 cut(s) 318
BsiHKCI CYCGRG 3 cut(s) 221, 422, 440
BsiSI CCGG 5 cut(s) 95, 254, 395, 542, 575
BslFI GGGAC 1 cut(s) 439
BslI CCNNNNNNNGG 1 cut(s) 561
BsmAI GTCTC 2 cut(s) 428, 624
BsmFI GGGAC 1 cut(s) 439
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 1 cut(s) 361
BsoBI CYCGRG 3 cut(s) 221, 422, 440
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 2 cut(s) 79, 391
BspACI CCGC 6 cut(s) 111, 377, 380, 486, 562, 565
BspANI GGCC 1 cut(s) 361
BspCNI CTCAG 1 cut(s) 301
BspFNI CGCG 3 cut(s) 377, 410, 486
BspLI GGNNCC 1 cut(s) 90
BspMI ACCTGC 1 cut(s) 176
BspPI GGATC 1 cut(s) 386
BspQI GCTCTTC 1 cut(s) 51
BspTI CTTAAG 1 cut(s) 172
BsrFI RCCGGY 1 cut(s) 541
BsrI ACTGG 2 cut(s) 147, 203
BssAI RCCGGY 1 cut(s) 541
BssECI CCNNGG 7 cut(s) 189, 222, 323, 324, 362, 441, 624
BssMI GATC 2 cut(s) 79, 391
BssT1I CCWWGG 2 cut(s) 362, 624
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 1 cut(s) 582
Bst6I CTCTTC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 172
BstC8I GCNNGC 3 cut(s) 285, 318, 572
BstDEI CTNAG 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 189
BstEII GGTNACC 1 cut(s) 550
BstF5I GGATG 2 cut(s) 129, 451
BstFNI CGCG 3 cut(s) 377, 410, 486
BstH2I RGCGCY 2 cut(s) 215, 571
BstHHI GCGC 4 cut(s) 214, 410, 486, 570
BstKTI GATC 2 cut(s) 82, 394
BstMAI GTCTC 2 cut(s) 428, 624
BstMBI GATC 2 cut(s) 79, 391
BstMCI CGRYCG 1 cut(s) 466
BstMWI GCNNNNNNNGC 4 cut(s) 293, 455, 531, 571
BstNI CCWGG 1 cut(s) 325
BstPI GGTNACC 1 cut(s) 550
BstSCI CCNGG 1 cut(s) 323
BstSLI GKGCMC 1 cut(s) 318
BstUI CGCG 3 cut(s) 377, 410, 486
BstV1I GCAGC 2 cut(s) 145, 457
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BsuRI GGCC 1 cut(s) 361
BtgI CCRYGG 1 cut(s) 189
BtsCI GGATG 2 cut(s) 129, 451
BveI ACCTGC 1 cut(s) 176
Cac8I GCNNGC 3 cut(s) 285, 318, 572
CfoI GCGC 4 cut(s) 214, 410, 486, 570
Cfr10I RCCGGY 1 cut(s) 541
CseI GACGC 1 cut(s) 285
Csp6I GTAC 3 cut(s) 4, 249, 373
CviQI GTAC 3 cut(s) 4, 249, 373
DdeI CTNAG 1 cut(s) 288
DpnI GATC 2 cut(s) 81, 393
DpnII GATC 2 cut(s) 79, 391
DriI GACNNNNNGTC 1 cut(s) 616
Eam1104I CTCTTC 1 cut(s) 51
Eam1105I GACNNNNNGTC 1 cut(s) 616
EarI CTCTTC 1 cut(s) 51
EciI GGCGGA 1 cut(s) 395
Eco130I CCWWGG 2 cut(s) 362, 624
Eco147I AGGCCT 1 cut(s) 361
Eco88I CYCGRG 3 cut(s) 221, 422, 440
Eco91I GGTNACC 1 cut(s) 550
EcoO65I GGTNACC 1 cut(s) 550
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 2 cut(s) 362, 624
ErhI CCWWGG 2 cut(s) 362, 624
FaiI YATR 2 cut(s) 84, 86
FaqI GGGAC 1 cut(s) 439
FauNDI CATATG 1 cut(s) 84
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 7 cut(s) 112, 159, 378, 471, 487, 563, 566
FokI GGATG 2 cut(s) 116, 458
Fsp4HI GCNGC 7 cut(s) 112, 159, 378, 471, 487, 563, 566
FspBI CTAG 2 cut(s) 20, 74
GlaI GCGC 4 cut(s) 213, 409, 485, 569
GluI GCNGC 7 cut(s) 112, 159, 378, 471, 487, 563, 566
HaeII RGCGCY 2 cut(s) 215, 571
HaeIII GGCC 1 cut(s) 361
HapII CCGG 5 cut(s) 95, 254, 395, 542, 575
HgaI GACGC 1 cut(s) 285
HhaI GCGC 4 cut(s) 214, 410, 486, 570
Hin6I GCGC 4 cut(s) 212, 408, 484, 568
HinP1I GCGC 4 cut(s) 212, 408, 484, 568
HinfI GANTC 8 cut(s) 23, 32, 53, 206, 227, 233, 436, 617
HpaII CCGG 5 cut(s) 95, 254, 395, 542, 575
Hpy166II GTNNAC 3 cut(s) 121, 251, 316
Hpy188I TCNGA 5 cut(s) 37, 232, 238, 291, 607
Hpy188III TCNNGA 3 cut(s) 50, 424, 440
Hpy8I GTNNAC 3 cut(s) 121, 251, 316
Hpy99I CGWCG 2 cut(s) 119, 301
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 1 cut(s) 582
HpyCH4IV ACGT 3 cut(s) 117, 307, 590
HpyCH4V TGCA 1 cut(s) 316
HpyF10VI GCNNNNNNNGC 4 cut(s) 293, 455, 531, 571
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 3 cut(s) 117, 307, 590
HspAI GCGC 4 cut(s) 212, 408, 484, 568
Kzo9I GATC 2 cut(s) 79, 391
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 88
Lsp1109I GCAGC 2 cut(s) 145, 457
LweI GCATC 2 cut(s) 436, 611
MaeI CTAG 2 cut(s) 20, 74
MaeII ACGT 3 cut(s) 117, 307, 590
MaeIII GTNAC 1 cut(s) 550
MalI GATC 2 cut(s) 81, 393
MboI GATC 2 cut(s) 79, 391
MboII GAAGA 3 cut(s) 38, 97, 412
MflI RGATCY 1 cut(s) 391
MhlI GDGCHC 1 cut(s) 318
MlyI GAGTC 4 cut(s) 32, 221, 430, 626
MmeI TCCRAC 1 cut(s) 60
MroXI GAANNNNTTC 1 cut(s) 57
MseI TTAA 3 cut(s) 173, 270, 303
MspCI CTTAAG 1 cut(s) 172
MspI CCGG 5 cut(s) 95, 254, 395, 542, 575
MspR9I CCNGG 1 cut(s) 325
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 1 cut(s) 325
MvnI CGCG 3 cut(s) 377, 410, 486
MwoI GCNNNNNNNGC 4 cut(s) 293, 455, 531, 571
NdeI CATATG 1 cut(s) 84
NdeII GATC 2 cut(s) 79, 391
NlaIV GGNNCC 1 cut(s) 90
PasI CCCWGGG 1 cut(s) 324
PceI AGGCCT 1 cut(s) 361
PciSI GCTCTTC 1 cut(s) 51
PcsI WCGNNNNNNNCGW 3 cut(s) 114, 429, 438
PctI GAATGC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 57
PfeI GAWTC 4 cut(s) 32, 53, 206, 233
PkrI GCNGC 7 cut(s) 113, 160, 379, 472, 488, 564, 567
PleI GAGTC 4 cut(s) 31, 221, 430, 625
PpsI GAGTC 4 cut(s) 31, 221, 430, 625
Psp6I CCWGG 1 cut(s) 323
PspEI GGTNACC 1 cut(s) 550
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 90
PsuI RGATCY 1 cut(s) 391
RsaI GTAC 3 cut(s) 5, 250, 374
RsaNI GTAC 3 cut(s) 4, 249, 373
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 3 cut(s) 173, 270, 303
SatI GCNGC 7 cut(s) 112, 159, 378, 471, 487, 563, 566
Sau3AI GATC 2 cut(s) 79, 391
SchI GAGTC 4 cut(s) 32, 221, 430, 626
ScrFI CCNGG 1 cut(s) 325
SduI GDGCHC 1 cut(s) 318
SetI ASST 9 cut(s) 72, 120, 170, 244, 270, 289, 310, 472, 593
SfaNI GCATC 2 cut(s) 436, 611
SmlI CTYRAG 1 cut(s) 172
SmoI CTYRAG 1 cut(s) 172
SseBI AGGCCT 1 cut(s) 361
SsiI CCGC 6 cut(s) 111, 377, 380, 486, 562, 565
SspMI CTAG 2 cut(s) 20, 74
StuI AGGCCT 1 cut(s) 361
StyD4I CCNGG 1 cut(s) 323
StyI CCWWGG 2 cut(s) 362, 624
TaaI ACNGT 1 cut(s) 582
TaiI ACGT 3 cut(s) 120, 310, 593
TaqI TCGA 2 cut(s) 61, 527
TaqII GACCGA 1 cut(s) 480
TatI WGTACW 1 cut(s) 248
TauI GCSGC 5 cut(s) 114, 380, 489, 565, 568
TfiI GAWTC 4 cut(s) 32, 53, 206, 233
Tru1I TTAA 3 cut(s) 173, 270, 303
Tru9I TTAA 3 cut(s) 173, 270, 303
TseI GCWGC 2 cut(s) 158, 470
TspGWI ACGGA 2 cut(s) 21, 206
Vha464I CTTAAG 1 cut(s) 172
VneI GTGCAC 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 120
XmnI GAANNNNTTC 1 cut(s) 57
XspI CTAG 2 cut(s) 20, 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.