Rh1BG171400

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004826
Physical Location & Seq
Forward (+)
27346484 .. 27364133
17650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG171400.1

Sequence Viewer

Length: 807 bp
ATGGCAGAAAGAAGCCAGTCCACTAGCCCAGCCCAAATCTGTCCACAAACCCTCACTGTTGTTCTCCCTTCCACCAGACGCCAACAACCGGCGGGCTTCCACCACCAACACACCACCGTTGTCGGGAAATCCATCCCTCGATCATACTTGGTAGAAATTAACATGTACGGACAGACAACCCTAGAGTCAGTAGATTCCGACTTGGCTCTTCTTGAATCCATTCGACAACACCTTCTAGGCGATCATATGGAGCCTCCGGCAACCATCTTCGGCGGCGACTTAGACGACATCATCCCCTTGTTTTTCCCCAGTTCTACTGCTGCTTCAACGTGCTTGAGTGGGCTTTTTACCACGGAGAACTGGTGGGAATCGGCGCTAAAACCCGAGGACTCTGAATCCGAAGCTGTAAGTACACCGGAGGAGGAGGAGGTTAAGTTGAGGAAGCAAGCTCAGAATGCGTCGGTTAAGCGTGATGTGCACGCACCCAGGGTGGAGAGGCATTTCAGGGGCGTGAGGAGGAGGCCTTGGGGGAAGTACGCGGCGGAGATTAGAGATCCGGCGAAGAATGGCGCGAGAGTGTGGCTCGGGACTTACGAGACTCCCGAGGACGCTGGTCTGGCTTACGACCGAGCTGCTTTCAAACTGCGCGGCTGTAAAGCCAAACTCAACTTTCCTCACTTGATTGGCTCGAACAGCGTTGAGCCGGTTAGGGTTACCCCGAAGCGGCGGCGCTCGCCGGAACAGTCCTCTACGTCGACCTCGTCGGCTTCGGAGGACAACGAGTCTCCCAAGGCAAAGAGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

268

Amino Acids

29.85

Weight (kDa)

8.41

Isoelectric Point (pI)

74.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 167 - 217 6.4e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 755
AccII CGCG 3 cut(s) 539, 572, 648
AciI CCGC 7 cut(s) 92, 273, 539, 542, 648, 724, 727
AclWI GGATC 1 cut(s) 548
AcyI GRCGYC 1 cut(s) 79
AfaI GTAC 3 cut(s) 167, 412, 536
AfiI CCNNNNNNNGG 3 cut(s) 88, 123, 723
AflIII ACRYGT 1 cut(s) 162
AgsI TTSAA 3 cut(s) 215, 327, 640
AhdI GACNNNNNGTC 1 cut(s) 781
AjnI CCWGG 1 cut(s) 485
AluBI AGCT 3 cut(s) 404, 449, 632
AluI AGCT 3 cut(s) 404, 449, 632
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 2 cut(s) 590, 789
Alw44I GTGCAC 1 cut(s) 476
AlwI GGATC 1 cut(s) 548
Ama87I CYCGRG 3 cut(s) 383, 584, 602
AoxI GGCC 1 cut(s) 521
ApaLI GTGCAC 1 cut(s) 476
ApeKI GCWGC 2 cut(s) 320, 632
Asp700I GAANNNNTTC 1 cut(s) 219
AspLEI GCGC 4 cut(s) 376, 572, 648, 732
AvaI CYCGRG 3 cut(s) 383, 584, 602
BaeGI GKGCMC 1 cut(s) 480
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 2 cut(s) 307, 619
BccI CCATC 2 cut(s) 140, 272
BcgI CGANNNNNNTGC 2 cut(s) 614, 648
BciT130I CCWGG 1 cut(s) 487
BcoDI GTCTC 2 cut(s) 590, 789
BfaI CTAG 3 cut(s) 24, 182, 236
BfoI RGCGCY 2 cut(s) 377, 733
BisI GCNGC 7 cut(s) 274, 321, 540, 633, 649, 725, 728
BlsI GCNGC 7 cut(s) 275, 322, 541, 634, 650, 726, 729
Bme1390I CCNGG 1 cut(s) 487
BmeRI GACNNNNNGTC 1 cut(s) 781
BmeT110I CYCGRG 3 cut(s) 383, 584, 602
BmiI GGNNCC 1 cut(s) 252
BmrFI CCNGG 1 cut(s) 487
BmrI ACTGGG 1 cut(s) 303
BmuI ACTGGG 1 cut(s) 303
BoxI GACNNNNGTC 1 cut(s) 612
BplI GAGNNNNNCTC 2 cut(s) 567, 599
BpuEI CTTGAG 1 cut(s) 355
BsaHI GRCGYC 1 cut(s) 79
BsaJI CCNNGG 7 cut(s) 351, 384, 485, 486, 524, 603, 789
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 4 cut(s) 347, 377, 419, 449
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 123, 723
Bse118I RCCGGY 2 cut(s) 88, 703
Bse1I ACTGG 3 cut(s) 16, 309, 365
BseBI CCWGG 1 cut(s) 487
BseDI CCNNGG 7 cut(s) 351, 384, 485, 486, 524, 603, 789
BseGI GGATG 2 cut(s) 132, 291
BseLI CCNNNNNNNGG 3 cut(s) 88, 123, 723
BseMII CTCAG 1 cut(s) 464
BseNI ACTGG 3 cut(s) 16, 309, 365
BseRI GAGGAG 5 cut(s) 434, 437, 440, 529, 532
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 2 cut(s) 307, 619
BseYI CCCAGC 1 cut(s) 28
Bsh1236I CGCG 3 cut(s) 539, 572, 648
Bsh1285I CGRYCG 1 cut(s) 628
BshFI GGCC 1 cut(s) 523
BsiEI CGRYCG 1 cut(s) 628
BsiHKAI GWGCWC 1 cut(s) 480
BsiHKCI CYCGRG 3 cut(s) 383, 584, 602
BsiSI CCGG 6 cut(s) 89, 257, 416, 557, 704, 737
BslFI GGGAC 1 cut(s) 601
BslI CCNNNNNNNGG 3 cut(s) 88, 123, 723
BsmAI GTCTC 2 cut(s) 590, 789
BsmFI GGGAC 1 cut(s) 601
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 1 cut(s) 523
BsoBI CYCGRG 3 cut(s) 383, 584, 602
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 3 cut(s) 140, 241, 553
BspACI CCGC 7 cut(s) 92, 273, 539, 542, 648, 724, 727
BspANI GGCC 1 cut(s) 523
BspCNI CTCAG 1 cut(s) 463
BspFNI CGCG 3 cut(s) 539, 572, 648
BspLI GGNNCC 1 cut(s) 252
BspPI GGATC 1 cut(s) 548
BspQI GCTCTTC 1 cut(s) 213
BsrFI RCCGGY 2 cut(s) 88, 703
BsrI ACTGG 3 cut(s) 16, 309, 365
BssAI RCCGGY 2 cut(s) 88, 703
BssECI CCNNGG 7 cut(s) 351, 384, 485, 486, 524, 603, 789
BssMI GATC 3 cut(s) 140, 241, 553
BssNI GRCGYC 1 cut(s) 79
BssT1I CCWWGG 2 cut(s) 524, 789
Bst2UI CCWGG 1 cut(s) 487
Bst4CI ACNGT 3 cut(s) 58, 118, 744
Bst6I CTCTTC 1 cut(s) 213
BstACI GRCGYC 1 cut(s) 79
BstC8I GCNNGC 4 cut(s) 94, 447, 480, 734
BstDEI CTNAG 2 cut(s) 280, 450
BstDSI CCRYGG 1 cut(s) 351
BstEII GGTNACC 1 cut(s) 712
BstF5I GGATG 2 cut(s) 132, 291
BstFNI CGCG 3 cut(s) 539, 572, 648
BstH2I RGCGCY 2 cut(s) 377, 733
BstHHI GCGC 4 cut(s) 376, 572, 648, 732
BstKTI GATC 3 cut(s) 143, 244, 556
BstMAI GTCTC 2 cut(s) 590, 789
BstMBI GATC 3 cut(s) 140, 241, 553
BstMCI CGRYCG 1 cut(s) 628
BstMWI GCNNNNNNNGC 5 cut(s) 455, 475, 617, 693, 733
BstNI CCWGG 1 cut(s) 487
BstNSI RCATGY 1 cut(s) 166
BstPAI GACNNNNGTC 1 cut(s) 612
BstPI GGTNACC 1 cut(s) 712
BstSCI CCNGG 1 cut(s) 485
BstSLI GKGCMC 1 cut(s) 480
BstUI CGCG 3 cut(s) 539, 572, 648
BstV1I GCAGC 2 cut(s) 307, 619
BstX2I RGATCY 1 cut(s) 553
BstYI RGATCY 1 cut(s) 553
BsuRI GGCC 1 cut(s) 523
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 2 cut(s) 132, 291
BtsIMutI CAGTG 1 cut(s) 54
Cac8I GCNNGC 4 cut(s) 94, 447, 480, 734
CfoI GCGC 4 cut(s) 376, 572, 648, 732
Cfr10I RCCGGY 2 cut(s) 88, 703
CseI GACGC 3 cut(s) 87, 447, 617
Csp6I GTAC 3 cut(s) 166, 411, 535
CviAII CATG 1 cut(s) 163
CviQI GTAC 3 cut(s) 166, 411, 535
DdeI CTNAG 2 cut(s) 280, 450
DpnI GATC 3 cut(s) 142, 243, 555
DpnII GATC 3 cut(s) 140, 241, 553
DriI GACNNNNNGTC 1 cut(s) 781
Eam1104I CTCTTC 1 cut(s) 213
Eam1105I GACNNNNNGTC 1 cut(s) 781
EarI CTCTTC 1 cut(s) 213
EciI GGCGGA 1 cut(s) 557
Eco130I CCWWGG 2 cut(s) 524, 789
Eco147I AGGCCT 1 cut(s) 523
Eco88I CYCGRG 3 cut(s) 383, 584, 602
Eco91I GGTNACC 1 cut(s) 712
EcoO65I GGTNACC 1 cut(s) 712
EcoRII CCWGG 1 cut(s) 485
EcoT14I CCWWGG 2 cut(s) 524, 789
ErhI CCWWGG 2 cut(s) 524, 789
FaeI CATG 1 cut(s) 166
FaiI YATR 4 cut(s) 145, 164, 246, 248
FaqI GGGAC 1 cut(s) 601
FatI CATG 1 cut(s) 162
FauI CCCGC 1 cut(s) 85
FauNDI CATATG 1 cut(s) 246
FblI GTMKAC 1 cut(s) 755
Fnu4HI GCNGC 7 cut(s) 274, 321, 540, 633, 649, 725, 728
FokI GGATG 2 cut(s) 119, 278
Fsp4HI GCNGC 7 cut(s) 274, 321, 540, 633, 649, 725, 728
FspBI CTAG 3 cut(s) 24, 182, 236
GlaI GCGC 4 cut(s) 375, 571, 647, 731
GluI GCNGC 7 cut(s) 274, 321, 540, 633, 649, 725, 728
GsaI CCCAGC 1 cut(s) 32
HaeII RGCGCY 2 cut(s) 377, 733
HaeIII GGCC 1 cut(s) 523
HapII CCGG 6 cut(s) 89, 257, 416, 557, 704, 737
HgaI GACGC 3 cut(s) 87, 447, 617
HhaI GCGC 4 cut(s) 376, 572, 648, 732
Hin1I GRCGYC 1 cut(s) 79
Hin1II CATG 1 cut(s) 166
Hin6I GCGC 4 cut(s) 374, 570, 646, 730
HinP1I GCGC 4 cut(s) 374, 570, 646, 730
HincII GTYRAC 1 cut(s) 756
HindII GTYRAC 1 cut(s) 756
HinfI GANTC 8 cut(s) 185, 194, 215, 368, 389, 395, 598, 782
HpaII CCGG 6 cut(s) 89, 257, 416, 557, 704, 737
Hpy166II GTNNAC 5 cut(s) 21, 44, 413, 478, 756
Hpy188I TCNGA 5 cut(s) 199, 394, 400, 453, 772
Hpy188III TCNNGA 4 cut(s) 124, 212, 586, 602
Hpy8I GTNNAC 5 cut(s) 21, 44, 413, 478, 756
Hpy99I CGWCG 3 cut(s) 463, 757, 766
HpyAV CCTTC 2 cut(s) 78, 242
HpyCH4III ACNGT 3 cut(s) 58, 118, 744
HpyCH4IV ACGT 2 cut(s) 329, 752
HpyCH4V TGCA 1 cut(s) 478
HpyF10VI GCNNNNNNNGC 5 cut(s) 455, 475, 617, 693, 733
HpyF3I CTNAG 2 cut(s) 280, 450
HpySE526I ACGT 2 cut(s) 329, 752
Hsp92I GRCGYC 1 cut(s) 79
Hsp92II CATG 1 cut(s) 166
HspAI GCGC 4 cut(s) 374, 570, 646, 730
Kzo9I GATC 3 cut(s) 140, 241, 553
LguI GCTCTTC 1 cut(s) 213
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 2 cut(s) 307, 619
MaeI CTAG 3 cut(s) 24, 182, 236
MaeII ACGT 2 cut(s) 329, 752
MaeIII GTNAC 1 cut(s) 712
MalI GATC 3 cut(s) 142, 243, 555
MboI GATC 3 cut(s) 140, 241, 553
MboII GAAGA 3 cut(s) 200, 259, 574
MflI RGATCY 1 cut(s) 553
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 1 cut(s) 156
MlyI GAGTC 4 cut(s) 194, 383, 592, 791
MmeI TCCRAC 1 cut(s) 222
MroXI GAANNNNTTC 1 cut(s) 219
MseI TTAA 3 cut(s) 159, 432, 465
MspI CCGG 6 cut(s) 89, 257, 416, 557, 704, 737
MspR9I CCNGG 1 cut(s) 487
Mva1269I GAATGC 1 cut(s) 460
MvaI CCWGG 1 cut(s) 487
MvnI CGCG 3 cut(s) 539, 572, 648
MwoI GCNNNNNNNGC 5 cut(s) 455, 475, 617, 693, 733
NdeI CATATG 1 cut(s) 246
NdeII GATC 3 cut(s) 140, 241, 553
NlaIII CATG 1 cut(s) 166
NlaIV GGNNCC 1 cut(s) 252
NspI RCATGY 1 cut(s) 166
PasI CCCWGGG 1 cut(s) 486
PceI AGGCCT 1 cut(s) 523
PciI ACATGT 1 cut(s) 162
PciSI GCTCTTC 1 cut(s) 213
PcsI WCGNNNNNNNCGW 3 cut(s) 591, 600, 758
PctI GAATGC 1 cut(s) 460
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 4 cut(s) 194, 215, 368, 395
PflFI GACNNNGTC 1 cut(s) 760
PkrI GCNGC 7 cut(s) 275, 322, 541, 634, 650, 726, 729
PleI GAGTC 4 cut(s) 193, 383, 592, 790
PpsI GAGTC 4 cut(s) 193, 383, 592, 790
PscI ACATGT 1 cut(s) 162
PshAI GACNNNNGTC 1 cut(s) 612
Psp6I CCWGG 1 cut(s) 485
PspEI GGTNACC 1 cut(s) 712
PspFI CCCAGC 1 cut(s) 28
PspGI CCWGG 1 cut(s) 485
PspN4I GGNNCC 1 cut(s) 252
PsuI RGATCY 1 cut(s) 553
PsyI GACNNNGTC 1 cut(s) 760
RsaI GTAC 3 cut(s) 167, 412, 536
RsaNI GTAC 3 cut(s) 166, 411, 535
SalI GTCGAC 1 cut(s) 754
SapI GCTCTTC 1 cut(s) 213
SaqAI TTAA 3 cut(s) 159, 432, 465
SatI GCNGC 7 cut(s) 274, 321, 540, 633, 649, 725, 728
Sau3AI GATC 3 cut(s) 140, 241, 553
SchI GAGTC 4 cut(s) 194, 383, 592, 791
ScrFI CCNGG 1 cut(s) 487
SduI GDGCHC 1 cut(s) 480
SetI ASST 8 cut(s) 234, 332, 406, 432, 451, 634, 755, 761
SmlI CTYRAG 1 cut(s) 334
SmoI CTYRAG 1 cut(s) 334
Sse9I AATT 1 cut(s) 156
SseBI AGGCCT 1 cut(s) 523
SsiI CCGC 7 cut(s) 92, 273, 539, 542, 648, 724, 727
SspMI CTAG 3 cut(s) 24, 182, 236
StuI AGGCCT 1 cut(s) 523
StyD4I CCNGG 1 cut(s) 485
StyI CCWWGG 2 cut(s) 524, 789
TaaI ACNGT 3 cut(s) 58, 118, 744
TaiI ACGT 2 cut(s) 332, 755
TaqI TCGA 4 cut(s) 139, 223, 689, 755
TaqII GACCGA 1 cut(s) 642
TasI AATT 1 cut(s) 156
TatI WGTACW 1 cut(s) 410
TauI GCSGC 5 cut(s) 276, 542, 651, 727, 730
TfiI GAWTC 4 cut(s) 194, 215, 368, 395
Tru1I TTAA 3 cut(s) 159, 432, 465
Tru9I TTAA 3 cut(s) 159, 432, 465
TscAI CASTG 1 cut(s) 61
TseI GCWGC 2 cut(s) 320, 632
TspGWI ACGGA 2 cut(s) 183, 368
TspRI CASTG 1 cut(s) 61
Tth111I GACNNNGTC 1 cut(s) 760
VneI GTGCAC 1 cut(s) 476
XceI RCATGY 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 755
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 3 cut(s) 24, 182, 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.