RLG00000028727

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004826
Physical Location & Seq
Reverse (-)
27076375 .. 27077019
645 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028727

Sequence Viewer

Length: 645 bp
ATGCACGGACAGACAACCCTAGAGTCAGTAGATTCGGACTTGGCTCTTCTTGAATCCATTCGACAACACCTTCTAGGCGATCATATGGAGCCTCCGGCAACCATCTTCGGCGGCTACGTAGACGACATCATCCCCTTGTTTTTCCCCAGTTCTACTACTGCTTCAACCTGCTTGAGTGGGCTTTTTACCACGGAGAACTGGTGGGAGTCGGCCCTAAAACCCGAGGACTCTGAATCCGAAACTGTAAGTACCCCGGAGGAGGAGGAGGTTAAGTTGAGGAAGCAAGCTCAGAATGCGTCGGTTAAGCGTGATGTGCACGCACCCAGGGTGGAGAGGCATTTCAGGGGCGTGAGGAGGAGGCCTTGGGGGAAGTACGCGGCGGAGATTAGAGATCCGGCGAAGAATGGCGCGAGAGTGTGGCTCGGGACTTACGAGACTCCCGAGGACGCTGGTCTGGCTTACGACCGAGCTGCTTTCAAGCTGCGCGGCCGTAAAGCCAAACTCAACTTTCCTCACTTGATTGGCTCGAACAGCGTTGAGCCGGTTAGAGTTACGCCGAAGCGGCGGCGGTCGCCGGAACAGTCCTCTACGTCGACCTCGTCGGCATCGGAGGACAACGAGTCTCCCAAGGCAAAGAGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AP2

Protein Analysis

215

Amino Acids

24.0

Weight (kDa)

7.89

Isoelectric Point (pI)

70.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 113 - 163 5.7e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 176
AccI GTMKAC 2 cut(s) 120, 593
AccII CGCG 3 cut(s) 377, 410, 486
AciI CCGC 7 cut(s) 111, 377, 380, 486, 562, 565, 568
AclWI GGATC 1 cut(s) 386
AcoI YGGCCR 1 cut(s) 487
AfaI GTAC 2 cut(s) 250, 374
AfiI CCNNNNNNNGG 1 cut(s) 259
AgsI TTSAA 3 cut(s) 53, 165, 478
AhdI GACNNNNNGTC 1 cut(s) 619
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 3 cut(s) 287, 470, 481
AluI AGCT 3 cut(s) 287, 470, 481
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 2 cut(s) 428, 627
Alw44I GTGCAC 1 cut(s) 314
AlwI GGATC 1 cut(s) 386
Ama87I CYCGRG 3 cut(s) 221, 422, 440
AoxI GGCC 3 cut(s) 210, 359, 487
ApaLI GTGCAC 1 cut(s) 314
ApeKI GCWGC 2 cut(s) 470, 481
Asp700I GAANNNNTTC 1 cut(s) 57
AspLEI GCGC 2 cut(s) 410, 486
AspS9I GGNCC 1 cut(s) 211
AsuC2I CCSGG 1 cut(s) 254
AvaI CYCGRG 3 cut(s) 221, 422, 440
BaeGI GKGCMC 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 318
BbvI GCAGC 2 cut(s) 457, 468
BccI CCATC 1 cut(s) 110
BceAI ACGGC 1 cut(s) 474
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 1 cut(s) 325
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 2 cut(s) 428, 627
BfaI CTAG 2 cut(s) 20, 74
BfuAI ACCTGC 1 cut(s) 176
BglI GCCNNNNNGGC 1 cut(s) 562
BisI GCNGC 7 cut(s) 112, 378, 471, 482, 487, 563, 566
BlsI GCNGC 7 cut(s) 113, 379, 472, 483, 488, 564, 567
Bme1390I CCNGG 2 cut(s) 254, 325
BmeRI GACNNNNNGTC 1 cut(s) 619
BmeT110I CYCGRG 3 cut(s) 221, 422, 440
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 2 cut(s) 254, 325
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 1 cut(s) 614
BmuI ACTGGG 1 cut(s) 141
BoxI GACNNNNGTC 1 cut(s) 450
BplI GAGNNNNNCTC 2 cut(s) 405, 437
BpuEI CTTGAG 1 cut(s) 193
BpuMI CCSGG 1 cut(s) 254
BsaAI YACGTR 1 cut(s) 118
BsaJI CCNNGG 8 cut(s) 189, 222, 252, 323, 324, 362, 441, 627
BsaXI ACNNNNNCTCC 4 cut(s) 185, 215, 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse118I RCCGGY 1 cut(s) 541
Bse1I ACTGG 2 cut(s) 147, 203
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 8 cut(s) 189, 222, 252, 323, 324, 362, 441, 627
BseGI GGATG 1 cut(s) 129
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 2 cut(s) 147, 203
BseRI GAGGAG 5 cut(s) 272, 275, 278, 367, 370
BseSI GKGCMC 1 cut(s) 318
BseX3I CGGCCG 1 cut(s) 487
BseXI GCAGC 2 cut(s) 457, 468
Bsh1236I CGCG 3 cut(s) 377, 410, 486
Bsh1285I CGRYCG 3 cut(s) 466, 490, 572
BshFI GGCC 3 cut(s) 212, 361, 489
BsiEI CGRYCG 3 cut(s) 466, 490, 572
BsiHKAI GWGCWC 1 cut(s) 318
BsiHKCI CYCGRG 3 cut(s) 221, 422, 440
BsiSI CCGG 5 cut(s) 95, 254, 395, 542, 575
BslFI GGGAC 1 cut(s) 439
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 2 cut(s) 428, 627
BsmFI GGGAC 1 cut(s) 439
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 3 cut(s) 212, 361, 489
BsoBI CYCGRG 3 cut(s) 221, 422, 440
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 2 cut(s) 79, 391
BspACI CCGC 7 cut(s) 111, 377, 380, 486, 562, 565, 568
BspANI GGCC 3 cut(s) 212, 361, 489
BspCNI CTCAG 1 cut(s) 301
BspFNI CGCG 3 cut(s) 377, 410, 486
BspLI GGNNCC 1 cut(s) 90
BspMI ACCTGC 1 cut(s) 176
BspPI GGATC 1 cut(s) 386
BspQI GCTCTTC 1 cut(s) 51
BsrFI RCCGGY 1 cut(s) 541
BsrI ACTGG 2 cut(s) 147, 203
BssAI RCCGGY 1 cut(s) 541
BssECI CCNNGG 8 cut(s) 189, 222, 252, 323, 324, 362, 441, 627
BssMI GATC 2 cut(s) 79, 391
BssT1I CCWWGG 2 cut(s) 362, 627
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 2 cut(s) 244, 582
Bst6I CTCTTC 1 cut(s) 51
BstBAI YACGTR 1 cut(s) 118
BstC8I GCNNGC 2 cut(s) 285, 318
BstDEI CTNAG 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 1 cut(s) 129
BstFNI CGCG 3 cut(s) 377, 410, 486
BstHHI GCGC 2 cut(s) 410, 486
BstKTI GATC 2 cut(s) 82, 394
BstMAI GTCTC 2 cut(s) 428, 627
BstMBI GATC 2 cut(s) 79, 391
BstMCI CGRYCG 3 cut(s) 466, 490, 572
BstMWI GCNNNNNNNGC 6 cut(s) 293, 313, 455, 531, 562, 571
BstNI CCWGG 1 cut(s) 325
BstPAI GACNNNNGTC 1 cut(s) 450
BstSCI CCNGG 2 cut(s) 252, 323
BstSLI GKGCMC 1 cut(s) 318
BstSNI TACGTA 1 cut(s) 118
BstUI CGCG 3 cut(s) 377, 410, 486
BstV1I GCAGC 2 cut(s) 457, 468
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BstZI CGGCCG 1 cut(s) 487
BsuRI GGCC 3 cut(s) 212, 361, 489
BtgI CCRYGG 1 cut(s) 189
BtsCI GGATG 1 cut(s) 129
BveI ACCTGC 1 cut(s) 176
Cac8I GCNNGC 2 cut(s) 285, 318
CfoI GCGC 2 cut(s) 410, 486
Cfr10I RCCGGY 1 cut(s) 541
Cfr13I GGNCC 1 cut(s) 211
CseI GACGC 2 cut(s) 285, 455
Csp6I GTAC 2 cut(s) 249, 373
CviQI GTAC 2 cut(s) 249, 373
DdeI CTNAG 1 cut(s) 288
DpnI GATC 2 cut(s) 81, 393
DpnII GATC 2 cut(s) 79, 391
DriI GACNNNNNGTC 1 cut(s) 619
EaeI YGGCCR 1 cut(s) 487
EagI CGGCCG 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 51
Eam1105I GACNNNNNGTC 1 cut(s) 619
EarI CTCTTC 1 cut(s) 51
EciI GGCGGA 1 cut(s) 395
EclXI CGGCCG 1 cut(s) 487
Eco105I TACGTA 1 cut(s) 118
Eco130I CCWWGG 2 cut(s) 362, 627
Eco147I AGGCCT 1 cut(s) 361
Eco52I CGGCCG 1 cut(s) 487
Eco88I CYCGRG 3 cut(s) 221, 422, 440
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 2 cut(s) 362, 627
ErhI CCWWGG 2 cut(s) 362, 627
FaiI YATR 2 cut(s) 84, 86
FaqI GGGAC 1 cut(s) 439
FauNDI CATATG 1 cut(s) 84
FblI GTMKAC 2 cut(s) 120, 593
Fnu4HI GCNGC 7 cut(s) 112, 378, 471, 482, 487, 563, 566
FokI GGATG 1 cut(s) 116
Fsp4HI GCNGC 7 cut(s) 112, 378, 471, 482, 487, 563, 566
FspBI CTAG 2 cut(s) 20, 74
GlaI GCGC 2 cut(s) 409, 485
GluI GCNGC 7 cut(s) 112, 378, 471, 482, 487, 563, 566
HaeIII GGCC 3 cut(s) 212, 361, 489
HapII CCGG 5 cut(s) 95, 254, 395, 542, 575
HgaI GACGC 2 cut(s) 285, 455
HhaI GCGC 2 cut(s) 410, 486
Hin6I GCGC 2 cut(s) 408, 484
HinP1I GCGC 2 cut(s) 408, 484
HincII GTYRAC 1 cut(s) 594
HindII GTYRAC 1 cut(s) 594
HinfI GANTC 8 cut(s) 23, 32, 53, 206, 227, 233, 436, 620
HpaII CCGG 5 cut(s) 95, 254, 395, 542, 575
Hpy166II GTNNAC 3 cut(s) 121, 316, 594
Hpy188I TCNGA 5 cut(s) 37, 232, 238, 291, 610
Hpy188III TCNNGA 3 cut(s) 50, 424, 440
Hpy8I GTNNAC 3 cut(s) 121, 316, 594
Hpy99I CGWCG 3 cut(s) 301, 595, 604
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 2 cut(s) 244, 582
HpyCH4IV ACGT 2 cut(s) 117, 590
HpyCH4V TGCA 2 cut(s) 4, 316
HpyF10VI GCNNNNNNNGC 6 cut(s) 293, 313, 455, 531, 562, 571
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 2 cut(s) 117, 590
HspAI GCGC 2 cut(s) 408, 484
Kzo9I GATC 2 cut(s) 79, 391
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 88
Lsp1109I GCAGC 2 cut(s) 457, 468
LweI GCATC 1 cut(s) 614
MaeI CTAG 2 cut(s) 20, 74
MaeII ACGT 2 cut(s) 117, 590
MaeIII GTNAC 1 cut(s) 550
MalI GATC 2 cut(s) 81, 393
MboI GATC 2 cut(s) 79, 391
MboII GAAGA 3 cut(s) 38, 97, 412
MflI RGATCY 1 cut(s) 391
MhlI GDGCHC 1 cut(s) 318
MlyI GAGTC 5 cut(s) 32, 215, 221, 430, 629
MroXI GAANNNNTTC 1 cut(s) 57
MseI TTAA 2 cut(s) 270, 303
MspI CCGG 5 cut(s) 95, 254, 395, 542, 575
MspR9I CCNGG 2 cut(s) 254, 325
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 1 cut(s) 325
MvnI CGCG 3 cut(s) 377, 410, 486
MwoI GCNNNNNNNGC 6 cut(s) 293, 313, 455, 531, 562, 571
NciI CCSGG 1 cut(s) 254
NdeI CATATG 1 cut(s) 84
NdeII GATC 2 cut(s) 79, 391
NlaIV GGNNCC 1 cut(s) 90
PasI CCCWGGG 1 cut(s) 324
PceI AGGCCT 1 cut(s) 361
PciSI GCTCTTC 1 cut(s) 51
PcsI WCGNNNNNNNCGW 4 cut(s) 114, 429, 438, 596
PctI GAATGC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 57
PfeI GAWTC 3 cut(s) 32, 53, 233
PflFI GACNNNGTC 1 cut(s) 598
PkrI GCNGC 7 cut(s) 113, 379, 472, 483, 488, 564, 567
PleI GAGTC 5 cut(s) 31, 214, 221, 430, 628
PpsI GAGTC 5 cut(s) 31, 214, 221, 430, 628
Ppu21I YACGTR 1 cut(s) 118
PshAI GACNNNNGTC 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 1 cut(s) 211
PsuI RGATCY 1 cut(s) 391
PsyI GACNNNGTC 1 cut(s) 598
RsaI GTAC 2 cut(s) 250, 374
RsaNI GTAC 2 cut(s) 249, 373
SalI GTCGAC 1 cut(s) 592
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 2 cut(s) 270, 303
SatI GCNGC 7 cut(s) 112, 378, 471, 482, 487, 563, 566
Sau3AI GATC 2 cut(s) 79, 391
Sau96I GGNCC 1 cut(s) 211
SchI GAGTC 5 cut(s) 32, 215, 221, 430, 629
ScrFI CCNGG 2 cut(s) 254, 325
SduI GDGCHC 1 cut(s) 318
SetI ASST 9 cut(s) 72, 120, 170, 270, 289, 472, 483, 593, 599
SfaNI GCATC 1 cut(s) 614
SmlI CTYRAG 1 cut(s) 172
SmoI CTYRAG 1 cut(s) 172
SnaBI TACGTA 1 cut(s) 118
SseBI AGGCCT 1 cut(s) 361
SsiI CCGC 7 cut(s) 111, 377, 380, 486, 562, 565, 568
SspMI CTAG 2 cut(s) 20, 74
StuI AGGCCT 1 cut(s) 361
StyD4I CCNGG 2 cut(s) 252, 323
StyI CCWWGG 2 cut(s) 362, 627
TaaI ACNGT 2 cut(s) 244, 582
TaiI ACGT 2 cut(s) 120, 593
TaqI TCGA 3 cut(s) 61, 527, 593
TaqII GACCGA 1 cut(s) 480
TauI GCSGC 5 cut(s) 114, 380, 489, 565, 568
TfiI GAWTC 3 cut(s) 32, 53, 233
Tru1I TTAA 2 cut(s) 270, 303
Tru9I TTAA 2 cut(s) 270, 303
TseI GCWGC 2 cut(s) 470, 481
TspGWI ACGGA 2 cut(s) 21, 206
Tth111I GACNNNGTC 1 cut(s) 598
VneI GTGCAC 1 cut(s) 314
XmiI GTMKAC 2 cut(s) 120, 593
XmnI GAANNNNTTC 1 cut(s) 57
XspI CTAG 2 cut(s) 20, 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.