RchiOBHm_Chr1g0367491

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003956
Physical Location & Seq
Forward (+)
57808046 .. 57809245
1200 bp
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UTR
Exon/CDS
Intron
PRQ59195

Sequence Viewer

Length: 681 bp
ATGGTGGCAAAGTTGAGGTTTCCCAGGTTGATGAGGACTAGAAGGAGGAAGCAGAAAGCCGTGGTTGGAATTTTTGCTTTTGAAGTTGCAAGTGTGATGTCTAAGCTGGTCCATCTATGGGCATTCCTGAGTTCTAAACAAGTTGATAGGCTCAGAAAAAAGATCTCAGATTCGGTGGGGATAAAAAAGCTTGTGTCACATGATGATGATTTCATTCGAGGTTTAATACCCGGTGAATTGTTTGAGAATATGGTCCCTTTGACAAAATATGTGGCCAGGCTCGGGAAGAATTATTGTTCTGATCCTAGTTTGAAGGATTTTGAGCATGCTGTTAGTGATTGGATCAACAATGGTGTTGACCCTTTTGGATGGGAACTTCCATGGGAGAAGATGGAAAAGAAAGCCAACAAGATGGAGAGGTTCATTTTAATAAATGCAAATTTGTATGACGGGATGAAACTTCTTTCGGATCTTGAACATACTTTGAACGACATCACTGCCACCTTGGATGGTTCAATTTTGCTTGAGTTTCAGAACAAGGTAGAGCTGAAGCGGCTTGAAGTGGAGAATTTGAAAGAGGAGTCTTTGTGGAACAGGACTTACGATTACGTAGGTATCCTTTTGGCAAGATCTGTCTTCACAATTTTCAGTTGGATCAAGTCTGTTTTTGGAGTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

226

Amino Acids

26.21

Weight (kDa)

9.3

Isoelectric Point (pI)

32.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 24 - 77 3.6e-21 Domain of unknown function (DUF3475)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 553
AclWI GGATC 4 cut(s) 296, 350, 477, 662
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 3 cut(s) 69, 439, 568
AcuI CTGAAG 1 cut(s) 569
AfiI CCNNNNNNNGG 2 cut(s) 118, 282
AgsI TTSAA 7 cut(s) 83, 313, 476, 487, 516, 560, 574
AjnI CCWGG 2 cut(s) 23, 275
AluBI AGCT 3 cut(s) 106, 190, 547
AluI AGCT 3 cut(s) 106, 190, 547
AlwI GGATC 4 cut(s) 296, 350, 477, 662
Ama87I CYCGRG 1 cut(s) 281
AoxI GGCC 1 cut(s) 273
ApoI RAATTY 3 cut(s) 69, 439, 568
AspS9I GGNCC 2 cut(s) 109, 253
AsuC2I CCSGG 1 cut(s) 231
AsuHPI GGTGA 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 2 cut(s) 109, 253
BaeI ACNNNNGTAYC 2 cut(s) 598, 631
BalI TGGCCA 1 cut(s) 275
BbsI GAAGAC 1 cut(s) 628
BccI CCATC 5 cut(s) 120, 363, 385, 406, 503
BceAI ACGGC 1 cut(s) 44
BcgI CGANNNNNNTGC 2 cut(s) 479, 513
BciT130I CCWGG 2 cut(s) 25, 277
BciVI GTATCC 1 cut(s) 626
BcnI CCSGG 1 cut(s) 231
BfaI CTAG 2 cut(s) 39, 306
BfuI GTATCC 1 cut(s) 626
BglII AGATCT 2 cut(s) 162, 629
BisI GCNGC 1 cut(s) 554
BlsI GCNGC 1 cut(s) 555
Bme1390I CCNGG 3 cut(s) 25, 231, 277
Bme18I GGWCC 2 cut(s) 109, 253
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 2 cut(s) 109, 253
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 3 cut(s) 25, 231, 277
BpiI GAAGAC 1 cut(s) 628
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 1 cut(s) 231
BsaAI YACGTR 1 cut(s) 610
BsaJI CCNNGG 4 cut(s) 23, 60, 380, 504
BsaXI ACNNNNNCTCC 2 cut(s) 572, 602
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 282
BseBI CCWGG 2 cut(s) 25, 277
BseDI CCNNGG 4 cut(s) 23, 60, 380, 504
BseGI GGATG 3 cut(s) 374, 459, 514
BseLI CCNNNNNNNGG 2 cut(s) 118, 282
BseMII CTCAG 3 cut(s) 119, 166, 180
BseRI GAGGAG 1 cut(s) 593
BshFI GGCC 1 cut(s) 275
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 2 cut(s) 118, 282
BsmFI GGGAC 1 cut(s) 239
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 1 cut(s) 275
BsoBI CYCGRG 1 cut(s) 281
Bsp143I GATC 6 cut(s) 162, 301, 342, 469, 629, 654
Bsp19I CCATGG 1 cut(s) 380
BspACI CCGC 1 cut(s) 553
BspANI GGCC 1 cut(s) 275
BspCNI CTCAG 3 cut(s) 120, 165, 179
BspLI GGNNCC 1 cut(s) 255
BspPI GGATC 4 cut(s) 296, 350, 477, 662
BssECI CCNNGG 4 cut(s) 23, 60, 380, 504
BssMI GATC 6 cut(s) 162, 301, 342, 469, 629, 654
BssT1I CCWWGG 2 cut(s) 380, 504
Bst2UI CCWGG 2 cut(s) 25, 277
BstBAI YACGTR 1 cut(s) 610
BstC8I GCNNGC 1 cut(s) 327
BstDEI CTNAG 4 cut(s) 102, 128, 152, 166
BstDSI CCRYGG 2 cut(s) 60, 380
BstF5I GGATG 3 cut(s) 374, 459, 514
BstKTI GATC 6 cut(s) 165, 304, 345, 472, 632, 657
BstMBI GATC 6 cut(s) 162, 301, 342, 469, 629, 654
BstMWI GCNNNNNNNGC 1 cut(s) 553
BstNI CCWGG 2 cut(s) 25, 277
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 3 cut(s) 23, 229, 275
BstSNI TACGTA 1 cut(s) 610
BstV2I GAAGAC 1 cut(s) 628
BstX2I RGATCY 3 cut(s) 162, 469, 629
BstXI CCANNNNNNTGG 1 cut(s) 412
BstYI RGATCY 3 cut(s) 162, 469, 629
BsuI GTATCC 1 cut(s) 626
BsuRI GGCC 1 cut(s) 275
BtgI CCRYGG 2 cut(s) 60, 380
BtsCI GGATG 3 cut(s) 374, 459, 514
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 1 cut(s) 495
Cac8I GCNNGC 1 cut(s) 327
Cfr13I GGNCC 2 cut(s) 109, 253
CspCI CAANNNNNGTGG 2 cut(s) 252, 287
CviAII CATG 3 cut(s) 200, 326, 381
CviJI RGCY 9 cut(s) 59, 106, 151, 190, 275, 280, 404, 547, 556
CviKI_1 RGCY 9 cut(s) 59, 106, 151, 190, 275, 280, 404, 547, 556
DdeI CTNAG 4 cut(s) 102, 128, 152, 166
DpnI GATC 6 cut(s) 164, 303, 344, 471, 631, 656
DpnII GATC 6 cut(s) 162, 301, 342, 469, 629, 654
EaeI YGGCCR 1 cut(s) 273
Eco105I TACGTA 1 cut(s) 610
Eco130I CCWWGG 2 cut(s) 380, 504
Eco47I GGWCC 2 cut(s) 109, 253
Eco57I CTGAAG 1 cut(s) 569
Eco88I CYCGRG 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 23, 275
EcoT14I CCWWGG 2 cut(s) 380, 504
ErhI CCWWGG 2 cut(s) 380, 504
FaeI CATG 3 cut(s) 203, 329, 384
FaiI YATR 8 cut(s) 118, 201, 251, 270, 327, 382, 447, 480
FaqI GGGAC 1 cut(s) 239
FatI CATG 3 cut(s) 199, 325, 380
Fnu4HI GCNGC 1 cut(s) 554
FokI GGATG 3 cut(s) 381, 466, 521
Fsp4HI GCNGC 1 cut(s) 554
FspBI CTAG 2 cut(s) 39, 306
GluI GCNGC 1 cut(s) 554
HaeIII GGCC 1 cut(s) 275
HapII CCGG 1 cut(s) 231
Hin1II CATG 3 cut(s) 203, 329, 384
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 2 cut(s) 170, 581
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 5 cut(s) 155, 169, 301, 469, 534
Hpy188III TCNNGA 3 cut(s) 127, 283, 473
Hpy8I GTNNAC 1 cut(s) 358
HpyAV CCTTC 2 cut(s) 36, 307
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 2 cut(s) 89, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 553
HpyF3I CTNAG 4 cut(s) 102, 128, 152, 166
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 3 cut(s) 203, 329, 384
Kzo9I GATC 6 cut(s) 162, 301, 342, 469, 629, 654
LpnPI CCDG 8 cut(s) 10, 37, 92, 140, 244, 262, 289, 580
MaeI CTAG 2 cut(s) 39, 306
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 1 cut(s) 195
MalI GATC 6 cut(s) 164, 303, 344, 471, 631, 656
MboI GATC 6 cut(s) 162, 301, 342, 469, 629, 654
MboII GAAGA 3 cut(s) 298, 400, 628
MflI RGATCY 3 cut(s) 162, 469, 629
MlsI TGGCCA 1 cut(s) 275
MluCI AATT 7 cut(s) 69, 236, 289, 439, 516, 568, 642
MluNI TGGCCA 1 cut(s) 275
MlyI GAGTC 1 cut(s) 590
MmeI TCCRAC 2 cut(s) 46, 632
MnlI CCTC 6 cut(s) 9, 27, 39, 212, 411, 571
Mox20I TGGCCA 1 cut(s) 275
MscI TGGCCA 1 cut(s) 275
MseI TTAA 3 cut(s) 224, 428, 679
MslI CAYNNNNRTG 1 cut(s) 204
Msp20I TGGCCA 1 cut(s) 275
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 3 cut(s) 25, 231, 277
Mva1269I GAATGC 1 cut(s) 122
MvaI CCWGG 2 cut(s) 25, 277
MwoI GCNNNNNNNGC 1 cut(s) 553
NciI CCSGG 1 cut(s) 231
NcoI CCATGG 1 cut(s) 380
NdeII GATC 6 cut(s) 162, 301, 342, 469, 629, 654
NlaIII CATG 3 cut(s) 203, 329, 384
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 1 cut(s) 195
NspI RCATGY 1 cut(s) 329
PaeI GCATGC 1 cut(s) 329
PctI GAATGC 1 cut(s) 122
PfeI GAWTC 1 cut(s) 170
PkrI GCNGC 1 cut(s) 555
PleI GAGTC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 589
Ppu21I YACGTR 1 cut(s) 610
Psp6I CCWGG 2 cut(s) 23, 275
PspGI CCWGG 2 cut(s) 23, 275
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 2 cut(s) 109, 253
PsrI GAACNNNNNNTAC 2 cut(s) 584, 616
PsuI RGATCY 3 cut(s) 162, 469, 629
RseI CAYNNNNRTG 1 cut(s) 204
SaqAI TTAA 3 cut(s) 224, 428, 679
SatI GCNGC 1 cut(s) 554
Sau3AI GATC 6 cut(s) 162, 301, 342, 469, 629, 654
Sau96I GGNCC 2 cut(s) 109, 253
SchI GAGTC 1 cut(s) 590
ScrFI CCNGG 3 cut(s) 25, 231, 277
SinI GGWCC 2 cut(s) 109, 253
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
SnaBI TACGTA 1 cut(s) 610
SphI GCATGC 1 cut(s) 329
Sse9I AATT 7 cut(s) 69, 236, 289, 439, 516, 568, 642
SsiI CCGC 1 cut(s) 553
SspMI CTAG 2 cut(s) 39, 306
StyD4I CCNGG 3 cut(s) 23, 229, 275
StyI CCWWGG 2 cut(s) 380, 504
TaiI ACGT 1 cut(s) 612
TaqI TCGA 1 cut(s) 217
TasI AATT 7 cut(s) 69, 236, 289, 439, 516, 568, 642
TauI GCSGC 1 cut(s) 556
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 3 cut(s) 224, 428, 679
Tru9I TTAA 3 cut(s) 224, 428, 679
TscAI CASTG 1 cut(s) 502
TseFI GTSAC 1 cut(s) 195
Tsp45I GTSAC 1 cut(s) 195
TspDTI ATGAA 3 cut(s) 202, 412, 470
TspRI CASTG 1 cut(s) 502
VpaK11BI GGWCC 2 cut(s) 109, 253
XapI RAATTY 3 cut(s) 69, 439, 568
XceI RCATGY 1 cut(s) 329
XspI CTAG 2 cut(s) 39, 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.