Rh1AG348700

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003956
Physical Location & Seq
Reverse (-)
58445604 .. 58447517
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG348700.1

Sequence Viewer

Length: 993 bp
ATGGGAAAGTTGTGGTTTCAAAGGACTACAAAGAAGAAGAATAAGTTCTTCAAAAAAGAGAAGCAGCAGAAAGTAGTGATTGGAGTTTTGGCCTTCGAAGTTGCAAGAGTCATGTCTAGGCTGCTCCATCTATGGACATCTCTAAGCGATGAACATGTTAACAGGCTCAGACAACATATTTTAAATTCCGTGGGTATAGAAAAGCTTGTGTCAGATGACAATGAGTTCACACTAGGTTTAATATCCAGCGAATTGTTTGAGAATGTGGTCACTGTGGCAAATTATGTGGCCAGGCTAGGGAAGAATCATTCTTCTCATCCTAGTTTGAAGGCTTTTCAGCATGCTATTAGTGATTGGATCCATAATGGTGTTGACCCTTATGGATGGCAACTTTCATGGGAGAAGATGGAAAGGGAGTCCAACATGATGAAGGAGTTCATTTCAATAAATGCAGATTTGTATGACAAGATGAAACTTCTTTCGGATCTTGAACATACTTTGAAAGACATCACTGCCACCTTAGATGGTCCAATTTTGCTTGAGTTTCAGAACAAGGTAGAGCTGAAGCGGCACGAGGTGGAGAATTTGAAAGAGGTCTCTCTGTGGAACACGACTTACGATTACGTAGGCATCCTTTTGGCAACATCTGTCTTCACAATTGCAACAATTAAAACAATAAAATGGGTATCACCGCTTGCTCATAATATGAAGAAATGGCAATCCAAGAGGAACATTCAACAGAAAGCCCAGAAAGCCTCTGTTTTCAATCATCCTGTGCTTCTGGTACAAACCCTCTACTTTGCAAATCATCAGAAGACAGAAGCAAAAATTACAGATCTTCTTGTTGGTATGCATTACACCTATAATCTTAAAAGACAAGTCAAGGCAAAATCTATGCTAGAGAGAGGCCGCAACTCGCTTAAACTAAAAGCAGCCAACTTAGAAGCCCAAGACGAAGCCCTGGTCTCCATTGACGGAGATCAAACTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

330

Amino Acids

38.09

Weight (kDa)

9.43

Isoelectric Point (pI)

34.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 29 - 84 1.7e-23 Domain of unknown function (DUF3475)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 568, 692, 910
AclWI GGATC 3 cut(s) 352, 365, 492
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 2 cut(s) 184, 583
AcuI CTGAAG 1 cut(s) 584
AdeI CACNNNGTG 1 cut(s) 577
AfaI GTAC 1 cut(s) 786
AfiI CCNNNNNNNGG 1 cut(s) 297
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 9 cut(s) 20, 52, 328, 444, 491, 502, 589, 737, 766
AjnI CCWGG 2 cut(s) 290, 960
AluBI AGCT 2 cut(s) 205, 562
AluI AGCT 2 cut(s) 205, 562
Alw26I GTCTC 2 cut(s) 601, 970
AlwI GGATC 3 cut(s) 352, 365, 492
AoxI GGCC 3 cut(s) 90, 288, 907
ApeKI GCWGC 3 cut(s) 64, 121, 932
ApoI RAATTY 2 cut(s) 184, 583
ArsI GACNNNNNNTTYG 2 cut(s) 948, 980
Asp700I GAANNNNTTC 2 cut(s) 44, 434
AspS9I GGNCC 1 cut(s) 527
AsuHPI GGTGA 1 cut(s) 681
AsuII TTCGAA 1 cut(s) 96
AvaII GGWCC 1 cut(s) 527
BalI TGGCCA 1 cut(s) 290
BamHI GGATCC 1 cut(s) 357
BauI CACGAG 1 cut(s) 572
BbsI GAAGAC 2 cut(s) 643, 821
BbvI GCAGC 3 cut(s) 76, 108, 944
BccI CCATC 4 cut(s) 135, 378, 400, 518
BciT130I CCWGG 2 cut(s) 292, 962
BcoDI GTCTC 2 cut(s) 601, 970
BfaI CTAG 5 cut(s) 117, 233, 296, 321, 899
BglII AGATCT 1 cut(s) 835
BisI GCNGC 5 cut(s) 65, 122, 569, 910, 933
BlsI GCNGC 5 cut(s) 66, 123, 570, 911, 934
Bme1390I CCNGG 2 cut(s) 292, 962
Bme18I GGWCC 1 cut(s) 527
BmgT120I GGNCC 1 cut(s) 527
BmiI GGNNCC 1 cut(s) 359
BmrFI CCNGG 2 cut(s) 292, 962
BmsI GCATC 1 cut(s) 639
BpiI GAAGAC 2 cut(s) 643, 821
Bpu14I TTCGAA 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 560
BsaAI YACGTR 1 cut(s) 625
BsaI GGTCTC 2 cut(s) 601, 970
BsaJI CCNNGG 2 cut(s) 189, 960
Bsc4I CCNNNNNNNGG 1 cut(s) 297
BseBI CCWGG 2 cut(s) 292, 962
BseDI CCNNGG 2 cut(s) 189, 960
BseGI GGATG 4 cut(s) 316, 389, 630, 769
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMII CTCAG 1 cut(s) 181
BseXI GCAGC 3 cut(s) 76, 108, 944
BshFI GGCC 3 cut(s) 92, 290, 909
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 2 cut(s) 601, 970
BsnI GGCC 3 cut(s) 92, 290, 909
Bso31I GGTCTC 2 cut(s) 601, 970
Bsp119I TTCGAA 1 cut(s) 96
Bsp143I GATC 4 cut(s) 357, 484, 835, 979
BspACI CCGC 3 cut(s) 568, 692, 910
BspANI GGCC 3 cut(s) 92, 290, 909
BspCNI CTCAG 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 359
BspPI GGATC 3 cut(s) 352, 365, 492
BspT104I TTCGAA 1 cut(s) 96
BspTNI GGTCTC 2 cut(s) 601, 970
BssECI CCNNGG 2 cut(s) 189, 960
BssMI GATC 4 cut(s) 357, 484, 835, 979
BssSI CACGAG 1 cut(s) 572
Bst2BI CACGAG 1 cut(s) 572
Bst2UI CCWGG 2 cut(s) 292, 962
Bst4CI ACNGT 2 cut(s) 274, 988
BstBAI YACGTR 1 cut(s) 625
BstBI TTCGAA 1 cut(s) 96
BstC8I GCNNGC 2 cut(s) 342, 696
BstDEI CTNAG 4 cut(s) 143, 167, 520, 940
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 4 cut(s) 316, 389, 630, 769
BstKTI GATC 4 cut(s) 360, 487, 838, 982
BstMAI GTCTC 2 cut(s) 601, 970
BstMBI GATC 4 cut(s) 357, 484, 835, 979
BstMWI GCNNNNNNNGC 2 cut(s) 568, 752
BstNI CCWGG 2 cut(s) 292, 962
BstNSI RCATGY 2 cut(s) 158, 344
BstSCI CCNGG 2 cut(s) 290, 960
BstSNI TACGTA 1 cut(s) 625
BstV1I GCAGC 3 cut(s) 76, 108, 944
BstV2I GAAGAC 2 cut(s) 643, 821
BstX2I RGATCY 3 cut(s) 357, 484, 835
BstYI RGATCY 3 cut(s) 357, 484, 835
BsuRI GGCC 3 cut(s) 92, 290, 909
BtgI CCRYGG 1 cut(s) 189
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 4 cut(s) 316, 389, 630, 769
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 2 cut(s) 270, 510
Cac8I GCNNGC 2 cut(s) 342, 696
Cfr13I GGNCC 1 cut(s) 527
Csp6I GTAC 1 cut(s) 785
CspCI CAANNNNNGTGG 2 cut(s) 267, 302
CviAII CATG 5 cut(s) 112, 155, 341, 396, 424
CviQI GTAC 1 cut(s) 785
DdeI CTNAG 4 cut(s) 143, 167, 520, 940
DpnI GATC 4 cut(s) 359, 486, 837, 981
DpnII GATC 4 cut(s) 357, 484, 835, 979
DraI TTTAAA 1 cut(s) 183
DraIII CACNNNGTG 1 cut(s) 577
EaeI YGGCCR 1 cut(s) 288
Eco105I TACGTA 1 cut(s) 625
Eco31I GGTCTC 2 cut(s) 601, 970
Eco47I GGWCC 1 cut(s) 527
Eco57I CTGAAG 1 cut(s) 584
EcoRII CCWGG 2 cut(s) 290, 960
EcoT22I ATGCAT 1 cut(s) 855
FaeI CATG 5 cut(s) 115, 158, 344, 399, 427
FatI CATG 5 cut(s) 111, 154, 340, 395, 423
Fnu4HI GCNGC 5 cut(s) 65, 122, 569, 910, 933
FokI GGATG 4 cut(s) 303, 396, 617, 756
Fsp4HI GCNGC 5 cut(s) 65, 122, 569, 910, 933
FspBI CTAG 5 cut(s) 117, 233, 296, 321, 899
GluI GCNGC 5 cut(s) 65, 122, 569, 910, 933
HaeIII GGCC 3 cut(s) 92, 290, 909
Hin1II CATG 5 cut(s) 115, 158, 344, 399, 427
HincII GTYRAC 2 cut(s) 160, 373
HindII GTYRAC 2 cut(s) 160, 373
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 3 cut(s) 108, 304, 416
HpaI GTTAAC 1 cut(s) 160
HphI GGTGA 1 cut(s) 681
Hpy166II GTNNAC 3 cut(s) 160, 228, 373
Hpy188I TCNGA 6 cut(s) 170, 214, 484, 549, 813, 992
Hpy188III TCNNGA 1 cut(s) 488
Hpy8I GTNNAC 3 cut(s) 160, 228, 373
HpyAV CCTTC 3 cut(s) 103, 322, 424
HpyCH4III ACNGT 2 cut(s) 274, 988
HpyCH4IV ACGT 1 cut(s) 624
HpyCH4V TGCA 5 cut(s) 104, 452, 662, 803, 853
HpyF10VI GCNNNNNNNGC 2 cut(s) 568, 752
HpyF3I CTNAG 4 cut(s) 143, 167, 520, 940
HpySE526I ACGT 1 cut(s) 624
Hsp92II CATG 5 cut(s) 115, 158, 344, 399, 427
KspAI GTTAAC 1 cut(s) 160
Kzo9I GATC 4 cut(s) 357, 484, 835, 979
LmnI GCTCC 1 cut(s) 129
LpnPI CCDG 9 cut(s) 148, 259, 277, 304, 761, 767, 786, 947, 974
Lsp1109I GCAGC 3 cut(s) 76, 108, 944
LweI GCATC 1 cut(s) 639
MaeI CTAG 5 cut(s) 117, 233, 296, 321, 899
MaeII ACGT 1 cut(s) 624
MaeIII GTNAC 1 cut(s) 268
MalI GATC 4 cut(s) 359, 486, 837, 981
MboI GATC 4 cut(s) 357, 484, 835, 979
MfeI CAATTG 1 cut(s) 657
MflI RGATCY 3 cut(s) 357, 484, 835
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 8 cut(s) 184, 251, 280, 531, 583, 657, 666, 828
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 2 cut(s) 117, 425
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 6 cut(s) 568, 586, 720, 766, 803, 899
Mox20I TGGCCA 1 cut(s) 290
Mph1103I ATGCAT 1 cut(s) 855
MroXI GAANNNNTTC 2 cut(s) 44, 434
MscI TGGCCA 1 cut(s) 290
MseI TTAA 6 cut(s) 159, 182, 239, 669, 870, 921
MslI CAYNNNNRTG 1 cut(s) 366
Msp20I TGGCCA 1 cut(s) 290
MspR9I CCNGG 2 cut(s) 292, 962
MunI CAATTG 1 cut(s) 657
MvaI CCWGG 2 cut(s) 292, 962
MwoI GCNNNNNNNGC 2 cut(s) 568, 752
NdeII GATC 4 cut(s) 357, 484, 835, 979
NlaIII CATG 5 cut(s) 115, 158, 344, 399, 427
NlaIV GGNNCC 1 cut(s) 359
NmuCI GTSAC 1 cut(s) 268
NsiI ATGCAT 1 cut(s) 855
NspI RCATGY 2 cut(s) 158, 344
NspV TTCGAA 1 cut(s) 96
PaeI GCATGC 1 cut(s) 344
PciI ACATGT 1 cut(s) 154
PdmI GAANNNNTTC 2 cut(s) 44, 434
PfeI GAWTC 1 cut(s) 304
PkrI GCNGC 5 cut(s) 66, 123, 570, 911, 934
PleI GAGTC 2 cut(s) 116, 424
PpsI GAGTC 2 cut(s) 116, 424
Ppu21I YACGTR 1 cut(s) 625
PscI ACATGT 1 cut(s) 154
Psp6I CCWGG 2 cut(s) 290, 960
PspGI CCWGG 2 cut(s) 290, 960
PspN4I GGNNCC 1 cut(s) 359
PspPI GGNCC 1 cut(s) 527
PsrI GAACNNNNNNTAC 2 cut(s) 599, 631
PsuI RGATCY 3 cut(s) 357, 484, 835
RsaI GTAC 1 cut(s) 786
RsaNI GTAC 1 cut(s) 785
RseI CAYNNNNRTG 1 cut(s) 366
SaqAI TTAA 6 cut(s) 159, 182, 239, 669, 870, 921
SatI GCNGC 5 cut(s) 65, 122, 569, 910, 933
Sau3AI GATC 4 cut(s) 357, 484, 835, 979
Sau96I GGNCC 1 cut(s) 527
SchI GAGTC 2 cut(s) 117, 425
ScrFI CCNGG 2 cut(s) 292, 962
SetI ASST 9 cut(s) 207, 238, 521, 558, 564, 579, 597, 627, 863
SfaNI GCATC 1 cut(s) 639
SfuI TTCGAA 1 cut(s) 96
SinI GGWCC 1 cut(s) 527
SmiMI CAYNNNNRTG 1 cut(s) 366
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
SnaBI TACGTA 1 cut(s) 625
SphI GCATGC 1 cut(s) 344
Sse9I AATT 8 cut(s) 184, 251, 280, 531, 583, 657, 666, 828
SsiI CCGC 3 cut(s) 568, 692, 910
SspMI CTAG 5 cut(s) 117, 233, 296, 321, 899
StyD4I CCNGG 2 cut(s) 290, 960
TaaI ACNGT 2 cut(s) 274, 988
TaiI ACGT 1 cut(s) 627
TaqI TCGA 1 cut(s) 96
TasI AATT 8 cut(s) 184, 251, 280, 531, 583, 657, 666, 828
TauI GCSGC 2 cut(s) 571, 912
TfiI GAWTC 1 cut(s) 304
Tru1I TTAA 6 cut(s) 159, 182, 239, 669, 870, 921
Tru9I TTAA 6 cut(s) 159, 182, 239, 669, 870, 921
TscAI CASTG 2 cut(s) 277, 517
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 3 cut(s) 64, 121, 932
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 6 cut(s) 165, 384, 427, 443, 485, 722
TspGWI ACGGA 2 cut(s) 178, 990
TspRI CASTG 2 cut(s) 277, 517
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 2 cut(s) 184, 583
XceI RCATGY 2 cut(s) 158, 344
XmnI GAANNNNTTC 2 cut(s) 44, 434
XspI CTAG 5 cut(s) 117, 233, 296, 321, 899
Zsp2I ATGCAT 1 cut(s) 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.