RLG00000027210

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003956
Physical Location & Seq
Forward (+)
9596549 .. 9597808
1260 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027210

Sequence Viewer

Length: 831 bp
ATGGGGGCAAAGTTGAGGTTTCCCAGGCTGTTGAGGACTAAAAGGAGGAAGCAGAAAGCAGTGGTTGGAATTTTTGCCTTTGAAGTTGCGAGTGTGATGTCTAAGCTGACAAAATATGTGGCCAGGCTTGGGAAGAATTATTTTTCTGATCCTAGTTCGAAGGATTTTGAGCATGCTGTTAGTGATTGGATCAACAATGGTGTTGACCCTTTTGGATGGGAACTTCCATGGGAGAAGATGGAAAAGAAAGCCAACAAGATGGAGAGATCTGATCACATTAATCCTAGTCCATCAATATTCAGCAAGTGCAAGCTGTTGGATGCTCCACCCGACACACTCGGTGCTGCTGCCTTAGCACTGCACTATGCAAATATTGTTATCAAAATTGAAAGTTATGTAAGATTTCCTCTCTTTTTATATGGTGGCAGACATAACCTGTACAGTATGTTGCCTGCAAATATGAGAGCTGAGCTAAGTGAAAGGCTGCCCTCCATCAAGAGCTCGACTTCATCAGTTTGCGATCTTGCAGCAGAGCGGAAGGTGGCAATGGAGGAAATATTAGAATGGTTAGCACCACTTGCTCATAATACGAAGAAATGGCAGTCCAAGAGGAACTTTCAACAGAAAGCCTCTGTTTCCAGGAGCCATGTGCTTCTGGTACAAACCCTCTACTTTGCAAATCAACAGATGACAGACGCAACAATTATGGAACTTCTTGTTGGTTTGCAGTACATCTACCAAGCTGATCTAGAAGCTTTCAGAGAGAATGACCGCCAAAGGCGTGAAAGAAAAGCAGCCCTCCATTGTTCTCTAGGAGTCCAAAATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

277

Amino Acids

31.6

Weight (kDa)

9.64

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF668 PF05003 112 - 199 9.1e-24 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 535
AciI CCGC 2 cut(s) 535, 772
AclWI GGATC 2 cut(s) 143, 197
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 341
AfaI GTAC 3 cut(s) 440, 660, 731
AfiI CCNNNNNNNGG 1 cut(s) 129
AgsI TTSAA 3 cut(s) 83, 389, 620
AjnI CCWGG 3 cut(s) 23, 122, 638
AjuI GAANNNNNNNTTGG 2 cut(s) 702, 734
AluBI AGCT 7 cut(s) 106, 313, 467, 472, 501, 743, 755
AluI AGCT 7 cut(s) 106, 313, 467, 472, 501, 743, 755
Alw21I GWGCWC 1 cut(s) 503
AlwI GGATC 2 cut(s) 143, 197
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 5 cut(s) 344, 347, 484, 527, 794
ApoI RAATTY 1 cut(s) 69
AseI ATTAAT 1 cut(s) 279
AsuII TTCGAA 1 cut(s) 158
BalI TGGCCA 1 cut(s) 122
BanII GRGCYC 1 cut(s) 503
Bbv12I GWGCWC 1 cut(s) 503
BbvI GCAGC 5 cut(s) 331, 334, 471, 539, 806
BccI CCATC 5 cut(s) 210, 232, 253, 298, 500
BciT130I CCWGG 3 cut(s) 25, 124, 640
BclI TGATCA 1 cut(s) 271
BfaI CTAG 4 cut(s) 153, 285, 749, 812
BglII AGATCT 1 cut(s) 266
BisI GCNGC 5 cut(s) 345, 348, 485, 528, 795
BlpI GCTNAGC 1 cut(s) 468
BlsI GCNGC 5 cut(s) 346, 349, 486, 529, 796
Bme1390I CCNGG 3 cut(s) 25, 124, 640
BmiI GGNNCC 1 cut(s) 644
BmrFI CCNGG 3 cut(s) 25, 124, 640
BmsI GCATC 1 cut(s) 310
Bpu10I CCTNAGC 1 cut(s) 352
Bpu1102I GCTNAGC 1 cut(s) 468
Bpu14I TTCGAA 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 23, 227
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse3DI GCAATG 1 cut(s) 552
BseBI CCWGG 3 cut(s) 25, 124, 640
BseDI CCNNGG 2 cut(s) 23, 227
BseGI GGATG 2 cut(s) 221, 325
BseLI CCNNNNNNNGG 1 cut(s) 129
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 1 cut(s) 459
BseXI GCAGC 5 cut(s) 331, 334, 471, 539, 806
BsgI GTGCAG 1 cut(s) 344
BshFI GGCC 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 503
BslI CCNNNNNNNGG 1 cut(s) 129
BsnI GGCC 1 cut(s) 122
Bsp119I TTCGAA 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 503
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 6 cut(s) 148, 189, 266, 271, 520, 745
Bsp1720I GCTNAGC 1 cut(s) 468
Bsp19I CCATGG 1 cut(s) 227
BspACI CCGC 2 cut(s) 535, 772
BspANI GGCC 1 cut(s) 122
BspCNI CTCAG 1 cut(s) 460
BspLI GGNNCC 1 cut(s) 644
BspPI GGATC 2 cut(s) 143, 197
BspT104I TTCGAA 1 cut(s) 158
BsrBI CCGCTC 1 cut(s) 535
BsrDI GCAATG 1 cut(s) 552
BsrGI TGTACA 1 cut(s) 438
BssECI CCNNGG 2 cut(s) 23, 227
BssMI GATC 6 cut(s) 148, 189, 266, 271, 520, 745
BssT1I CCWWGG 1 cut(s) 227
Bst2UI CCWGG 3 cut(s) 25, 124, 640
Bst4CI ACNGT 1 cut(s) 443
BstAPI GCANNNNNTGC 1 cut(s) 578
BstAUI TGTACA 1 cut(s) 438
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 174, 311, 453
BstDEI CTNAG 4 cut(s) 102, 352, 468, 473
BstDSI CCRYGG 1 cut(s) 227
BstF5I GGATG 2 cut(s) 221, 325
BstKTI GATC 6 cut(s) 151, 192, 269, 274, 523, 748
BstMBI GATC 6 cut(s) 148, 189, 266, 271, 520, 745
BstMWI GCNNNNNNNGC 2 cut(s) 353, 578
BstNI CCWGG 3 cut(s) 25, 124, 640
BstNSI RCATGY 1 cut(s) 176
BstSCI CCNGG 3 cut(s) 23, 122, 638
BstV1I GCAGC 5 cut(s) 331, 334, 471, 539, 806
BstX2I RGATCY 1 cut(s) 266
BstXI CCANNNNNNTGG 1 cut(s) 259
BstYI RGATCY 1 cut(s) 266
BsuRI GGCC 1 cut(s) 122
BtgI CCRYGG 1 cut(s) 227
BtsCI GGATG 2 cut(s) 221, 325
BtsI GCAGTG 2 cut(s) 66, 356
BtsIMutI CAGTG 2 cut(s) 66, 356
Cac8I GCNNGC 3 cut(s) 174, 311, 453
CseI GACGC 1 cut(s) 704
Csp6I GTAC 3 cut(s) 439, 659, 730
CspCI CAANNNNNGTGG 2 cut(s) 99, 134
CviAII CATG 3 cut(s) 173, 228, 647
CviQI GTAC 3 cut(s) 439, 659, 730
DdeI CTNAG 4 cut(s) 102, 352, 468, 473
DpnI GATC 6 cut(s) 150, 191, 268, 273, 522, 747
DpnII GATC 6 cut(s) 148, 189, 266, 271, 520, 745
DraIII CACNNNGTG 1 cut(s) 341
EaeI YGGCCR 1 cut(s) 120
Ecl136II GAGCTC 1 cut(s) 501
Eco130I CCWWGG 1 cut(s) 227
Eco24I GRGCYC 1 cut(s) 503
Eco53kI GAGCTC 1 cut(s) 501
EcoICRI GAGCTC 1 cut(s) 501
EcoRII CCWGG 3 cut(s) 23, 122, 638
EcoT14I CCWWGG 1 cut(s) 227
EcoT38I GRGCYC 1 cut(s) 503
ErhI CCWWGG 1 cut(s) 227
FaeI CATG 3 cut(s) 176, 231, 650
FalI AAGNNNNNCTT 2 cut(s) 599, 631
FatI CATG 3 cut(s) 172, 227, 646
FbaI TGATCA 1 cut(s) 271
Fnu4HI GCNGC 5 cut(s) 345, 348, 485, 528, 795
FokI GGATG 2 cut(s) 228, 332
FriOI GRGCYC 1 cut(s) 503
Fsp4HI GCNGC 5 cut(s) 345, 348, 485, 528, 795
FspBI CTAG 4 cut(s) 153, 285, 749, 812
GluI GCNGC 5 cut(s) 345, 348, 485, 528, 795
HaeIII GGCC 1 cut(s) 122
HgaI GACGC 1 cut(s) 704
Hin1II CATG 3 cut(s) 176, 231, 650
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 753
HinfI GANTC 1 cut(s) 816
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 3 cut(s) 148, 271, 761
Hpy188III TCNNGA 2 cut(s) 496, 749
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 2 cut(s) 154, 532
HpyCH4III ACNGT 1 cut(s) 443
HpyCH4V TGCA 7 cut(s) 309, 361, 368, 455, 527, 677, 727
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 578
HpyF3I CTNAG 4 cut(s) 102, 352, 468, 473
Hsp92II CATG 3 cut(s) 176, 231, 650
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 6 cut(s) 148, 189, 266, 271, 520, 745
LmnI GCTCC 2 cut(s) 328, 642
LpnPI CCDG 9 cut(s) 10, 37, 109, 136, 449, 465, 625, 641, 652
Lsp1109I GCAGC 5 cut(s) 331, 334, 471, 539, 806
LweI GCATC 1 cut(s) 310
MaeI CTAG 4 cut(s) 153, 285, 749, 812
MalI GATC 6 cut(s) 150, 191, 268, 273, 522, 747
MbiI CCGCTC 1 cut(s) 535
MboI GATC 6 cut(s) 148, 189, 266, 271, 520, 745
MboII GAAGA 3 cut(s) 145, 247, 604
MflI RGATCY 1 cut(s) 266
MhlI GDGCHC 1 cut(s) 503
MlsI TGGCCA 1 cut(s) 122
MluCI AATT 4 cut(s) 69, 136, 384, 702
MluNI TGGCCA 1 cut(s) 122
MlyI GAGTC 1 cut(s) 825
MmeI TCCRAC 2 cut(s) 46, 297
Mox20I TGGCCA 1 cut(s) 122
MscI TGGCCA 1 cut(s) 122
MseI TTAA 1 cut(s) 279
Msp20I TGGCCA 1 cut(s) 122
MspR9I CCNGG 3 cut(s) 25, 124, 640
MvaI CCWGG 3 cut(s) 25, 124, 640
MwoI GCNNNNNNNGC 2 cut(s) 353, 578
NcoI CCATGG 1 cut(s) 227
NdeII GATC 6 cut(s) 148, 189, 266, 271, 520, 745
NlaIII CATG 3 cut(s) 176, 231, 650
NlaIV GGNNCC 1 cut(s) 644
NspI RCATGY 1 cut(s) 176
NspV TTCGAA 1 cut(s) 158
PaeI GCATGC 1 cut(s) 176
PfoI TCCNGGA 1 cut(s) 638
PkrI GCNGC 5 cut(s) 346, 349, 486, 529, 796
PleI GAGTC 1 cut(s) 824
PpsI GAGTC 1 cut(s) 824
PshBI ATTAAT 1 cut(s) 279
Psp124BI GAGCTC 1 cut(s) 503
Psp6I CCWGG 3 cut(s) 23, 122, 638
PspGI CCWGG 3 cut(s) 23, 122, 638
PspN4I GGNNCC 1 cut(s) 644
PsuI RGATCY 1 cut(s) 266
RsaI GTAC 3 cut(s) 440, 660, 731
RsaNI GTAC 3 cut(s) 439, 659, 730
SacI GAGCTC 1 cut(s) 503
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 5 cut(s) 345, 348, 485, 528, 795
Sau3AI GATC 6 cut(s) 148, 189, 266, 271, 520, 745
SchI GAGTC 1 cut(s) 825
ScrFI CCNGG 3 cut(s) 25, 124, 640
SduI GDGCHC 1 cut(s) 503
SfaNI GCATC 1 cut(s) 310
SfuI TTCGAA 1 cut(s) 158
SphI GCATGC 1 cut(s) 176
Sse9I AATT 4 cut(s) 69, 136, 384, 702
SsiI CCGC 2 cut(s) 535, 772
SspI AATATT 3 cut(s) 297, 373, 558
SspMI CTAG 4 cut(s) 153, 285, 749, 812
SstI GAGCTC 1 cut(s) 503
StyD4I CCNGG 3 cut(s) 23, 122, 638
StyI CCWWGG 1 cut(s) 227
TaaI ACNGT 1 cut(s) 443
TaqI TCGA 2 cut(s) 158, 503
TasI AATT 4 cut(s) 69, 136, 384, 702
TatI WGTACW 2 cut(s) 438, 729
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 2 cut(s) 66, 363
TseI GCWGC 5 cut(s) 344, 347, 484, 527, 794
TspDTI ATGAA 1 cut(s) 498
TspRI CASTG 2 cut(s) 66, 363
VspI ATTAAT 1 cut(s) 279
XapI RAATTY 1 cut(s) 69
XbaI TCTAGA 1 cut(s) 748
XceI RCATGY 1 cut(s) 176
XspI CTAG 4 cut(s) 153, 285, 749, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.