Rmu_sc0002709.1_g000014

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002709.1
OG0003956
Physical Location & Seq
Forward (+)
59480 .. 59839
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002709.1_g000014.1.cds

Sequence Viewer

Length: 360 bp
atgagagctgagctaagtgaaaggctgccctctatcaagagctcgacttcatcagttcgcgatcttgcagcagagcggaaggtggcaatggcggaaatattagaatggttagcacctcttgctcataatacgacgaaatggcagtccaagaggaactttcaacagaaagcctctgtttccaggagccatgtgcttctggtacaaaccctctactttgcaaatcaacagaagacagaagcaacaattatggaacttcttgttggtttgcagtacatctaccaagctgatctagaagctttccgagagaatgaccgccgaaggagtgaaagaaaagcagccttcctgggagaccaagagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

119

Amino Acids

13.74

Weight (kDa)

9.39

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 76
AccII CGCG 1 cut(s) 60
AciI CCGC 3 cut(s) 76, 92, 313
AfaI GTAC 2 cut(s) 201, 272
AgsI TTSAA 1 cut(s) 161
AjnI CCWGG 2 cut(s) 179, 342
AjuI GAANNNNNNNTTGG 2 cut(s) 243, 275
AluBI AGCT 5 cut(s) 8, 13, 42, 284, 296
AluI AGCT 5 cut(s) 8, 13, 42, 284, 296
Alw21I GWGCWC 1 cut(s) 44
Alw26I GTCTC 1 cut(s) 342
ApeKI GCWGC 3 cut(s) 25, 68, 335
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
BanII GRGCYC 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 236
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 3 cut(s) 12, 80, 347
BciT130I CCWGG 2 cut(s) 181, 344
BcoDI GTCTC 1 cut(s) 342
BfaI CTAG 1 cut(s) 290
BisI GCNGC 3 cut(s) 26, 69, 336
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 3 cut(s) 27, 70, 337
Bme1390I CCNGG 2 cut(s) 181, 344
BmiI GGNNCC 1 cut(s) 185
BmrFI CCNGG 2 cut(s) 181, 344
BpiI GAAGAC 1 cut(s) 236
Bpu1102I GCTNAGC 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 343
Bse3DI GCAATG 1 cut(s) 93
BseBI CCWGG 2 cut(s) 181, 344
BseDI CCNNGG 1 cut(s) 343
BseMI GCAATG 1 cut(s) 93
BseXI GCAGC 3 cut(s) 12, 80, 347
Bsh1236I CGCG 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 44
BsmAI GTCTC 1 cut(s) 342
Bso31I GGTCTC 1 cut(s) 342
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 2 cut(s) 61, 286
Bsp1720I GCTNAGC 1 cut(s) 9
Bsp68I TCGCGA 1 cut(s) 60
BspACI CCGC 3 cut(s) 76, 92, 313
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 342
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 343
BssMI GATC 2 cut(s) 61, 286
Bst2UI CCWGG 2 cut(s) 181, 344
BstAPI GCANNNNNTGC 1 cut(s) 119
BstDEI CTNAG 2 cut(s) 9, 14
BstFNI CGCG 1 cut(s) 60
BstKTI GATC 2 cut(s) 64, 289
BstMAI GTCTC 1 cut(s) 342
BstMBI GATC 2 cut(s) 61, 286
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 2 cut(s) 181, 344
BstSCI CCNGG 2 cut(s) 179, 342
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 3 cut(s) 12, 80, 347
BstV2I GAAGAC 1 cut(s) 236
BtuMI TCGCGA 1 cut(s) 60
Csp6I GTAC 2 cut(s) 200, 271
CviAII CATG 1 cut(s) 188
CviJI RGCY 9 cut(s) 8, 13, 25, 42, 170, 186, 284, 296, 338
CviKI_1 RGCY 9 cut(s) 8, 13, 25, 42, 170, 186, 284, 296, 338
CviQI GTAC 2 cut(s) 200, 271
DdeI CTNAG 2 cut(s) 9, 14
DpnI GATC 2 cut(s) 63, 288
DpnII GATC 2 cut(s) 61, 286
EciI GGCGGA 1 cut(s) 107
Ecl136II GAGCTC 1 cut(s) 42
Eco24I GRGCYC 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 342
Eco53kI GAGCTC 1 cut(s) 42
EcoICRI GAGCTC 1 cut(s) 42
EcoRII CCWGG 2 cut(s) 179, 342
EcoT38I GRGCYC 1 cut(s) 44
FaeI CATG 1 cut(s) 191
FaiI YATR 3 cut(s) 126, 189, 248
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FatI CATG 1 cut(s) 187
Fnu4HI GCNGC 3 cut(s) 26, 69, 336
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 3 cut(s) 26, 69, 336
FspBI CTAG 1 cut(s) 290
GluI GCNGC 3 cut(s) 26, 69, 336
Hin1II CATG 1 cut(s) 191
HindIII AAGCTT 1 cut(s) 294
Hpy188I TCNGA 1 cut(s) 302
Hpy188III TCNNGA 3 cut(s) 37, 59, 290
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 3 cut(s) 73, 312, 349
HpyCH4V TGCA 3 cut(s) 68, 218, 268
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 2 cut(s) 9, 14
Hsp92II CATG 1 cut(s) 191
Kzo9I GATC 2 cut(s) 61, 286
LmnI GCTCC 1 cut(s) 183
LpnPI CCDG 5 cut(s) 166, 182, 193, 329, 356
Lsp1109I GCAGC 3 cut(s) 12, 80, 347
MaeI CTAG 1 cut(s) 290
MalI GATC 2 cut(s) 63, 288
MbiI CCGCTC 1 cut(s) 76
MboI GATC 2 cut(s) 61, 286
MboII GAAGA 1 cut(s) 241
MhlI GDGCHC 1 cut(s) 44
MluCI AATT 1 cut(s) 243
MnlI CCTC 5 cut(s) 40, 126, 144, 181, 218
MspR9I CCNGG 2 cut(s) 181, 344
MvaI CCWGG 2 cut(s) 181, 344
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 1 cut(s) 119
NdeII GATC 2 cut(s) 61, 286
NlaIII CATG 1 cut(s) 191
NlaIV GGNNCC 1 cut(s) 185
NruI TCGCGA 1 cut(s) 60
PfoI TCCNGGA 1 cut(s) 179
PkrI GCNGC 3 cut(s) 27, 70, 337
Psp124BI GAGCTC 1 cut(s) 44
Psp6I CCWGG 2 cut(s) 179, 342
PspGI CCWGG 2 cut(s) 179, 342
PspN4I GGNNCC 1 cut(s) 185
RruI TCGCGA 1 cut(s) 60
RsaI GTAC 2 cut(s) 201, 272
RsaNI GTAC 2 cut(s) 200, 271
SacI GAGCTC 1 cut(s) 44
SatI GCNGC 3 cut(s) 26, 69, 336
Sau3AI GATC 2 cut(s) 61, 286
ScrFI CCNGG 2 cut(s) 181, 344
SduI GDGCHC 1 cut(s) 44
SetI ASST 7 cut(s) 10, 15, 44, 84, 118, 286, 298
Sse9I AATT 1 cut(s) 243
SsiI CCGC 3 cut(s) 76, 92, 313
SspI AATATT 1 cut(s) 99
SspMI CTAG 1 cut(s) 290
SstI GAGCTC 1 cut(s) 44
StyD4I CCNGG 2 cut(s) 179, 342
TaqI TCGA 1 cut(s) 44
TasI AATT 1 cut(s) 243
TatI WGTACW 1 cut(s) 270
TseI GCWGC 3 cut(s) 25, 68, 335
TspDTI ATGAA 1 cut(s) 39
XbaI TCTAGA 1 cut(s) 289
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.