Rroxscaffold_2G00125250

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003956
Physical Location & Seq
Forward (+)
59923985 .. 59924884
900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00125250.1

Sequence Viewer

Length: 900 bp
ATGGGAACTTCCATGGGACTATGGGAGAAGATGGAAAAGAAAGCCAACAAGATAGAGAGGTTCATTTCAATAAATGCAAATTTGTATGACGGGATGAAACTTCTTTTGGATCTTGAACATACTTTGAACGACATCACTGCCACCTTGGATGGTCCAATTTTGCTTGAGTTTCAGAACAAGGTAGAGCTGAAGCGGCTTGAAGTGGAGAATTTGAAAGAGGAGTCTTTGTGGAACAGGACTTACGATTACGTAGGTATCCTTTTGGCAAGATCTGTCTTCACAATTTTCAGTTGGATCAAGTCTGTTTTTGGAGTTCCTCAACTGATGGCAGATGCAGAAACCAAAGATTCTGTTCACATTAGTCCTAGTCCATCAATTTTCAGCAAGTACAAGCTGTTGGATTTTCCACCCGACACACTCGGTGTTGCCGCCTTAGCACTGCACTATGCAAATATTGTTATCAAAATTGAAAGTTACGTAAGATTTCCTCTCTTCTTATATGGTGGCAGACATAACCTGTACAGTATGTTGCCTGCAAATATGAGAGCTGAGCTAAGTGAAAGGCTGCCCTCTATCTGGAGCTCGACTTCATCAGTTCGCGATCTTGCAGCAGAGCGGAAGGTGGCAATGGCGGAAATATTAGAATGGTTAGCACCACTTGCTCATAATACAACGAAATGGCAGTCCAAGAGGAACTTTCAACAGAAAGCCTCTGTTTCCAGGAGCCATGTGCTTCTGGTACAAACCCTCTACTTTGCAAATCAAGAGAAAACAGAAGCAACAATTATGGAACTTCTTGTTGGTTTGCAGTACATCTACCAAGCTGATCTAGAAGCTTTCCGAGAGAATGACCGCCGAAGGCGTGAAAGAGAAGCAGCCTCCCTGGAAGACCAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

299

Amino Acids

34.4

Weight (kDa)

6.13

Isoelectric Point (pI)

54.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF668 PF05003 139 - 226 3.5e-24 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 616
AccII CGCG 1 cut(s) 600
AciI CCGC 5 cut(s) 193, 429, 616, 632, 853
AclWI GGATC 2 cut(s) 117, 302
AcsI RAATTY 2 cut(s) 79, 208
AcuI CTGAAG 1 cut(s) 209
AdeI CACNNNGTG 1 cut(s) 422
AfaI GTAC 4 cut(s) 389, 521, 741, 812
AfiI CCNNNNNNNGG 1 cut(s) 576
AgsI TTSAA 7 cut(s) 69, 116, 127, 200, 214, 470, 701
AjnI CCWGG 2 cut(s) 719, 882
AjuI GAANNNNNNNTTGG 4 cut(s) 89, 121, 783, 815
AluBI AGCT 7 cut(s) 187, 394, 548, 553, 582, 824, 836
AluI AGCT 7 cut(s) 187, 394, 548, 553, 582, 824, 836
Alw21I GWGCWC 1 cut(s) 584
AlwI GGATC 2 cut(s) 117, 302
ApeKI GCWGC 3 cut(s) 565, 608, 875
ApoI RAATTY 2 cut(s) 79, 208
ArsI GACNNNNNNTTYG 2 cut(s) 668, 700
AspS9I GGNCC 1 cut(s) 152
AvaII GGWCC 1 cut(s) 152
BaeI ACNNNNGTAYC 2 cut(s) 238, 271
BanII GRGCYC 1 cut(s) 584
BbsI GAAGAC 2 cut(s) 268, 894
Bbv12I GWGCWC 1 cut(s) 584
BbvI GCAGC 3 cut(s) 552, 620, 887
BccI CCATC 4 cut(s) 25, 143, 319, 379
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciT130I CCWGG 2 cut(s) 721, 884
BciVI GTATCC 1 cut(s) 266
BfaI CTAG 2 cut(s) 366, 830
BfuI GTATCC 1 cut(s) 266
BglII AGATCT 1 cut(s) 269
BisI GCNGC 5 cut(s) 194, 429, 566, 609, 876
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 5 cut(s) 195, 430, 567, 610, 877
Bme1390I CCNGG 2 cut(s) 721, 884
Bme18I GGWCC 1 cut(s) 152
BmgT120I GGNCC 1 cut(s) 152
BmiI GGNNCC 1 cut(s) 725
BmrFI CCNGG 2 cut(s) 721, 884
BmsI GCATC 1 cut(s) 322
BpiI GAAGAC 2 cut(s) 268, 894
BpmI CTGGAG 1 cut(s) 598
Bpu10I CCTNAGC 1 cut(s) 433
Bpu1102I GCTNAGC 1 cut(s) 549
BpuEI CTTGAG 1 cut(s) 185
BsaAI YACGTR 2 cut(s) 250, 478
BsaJI CCNNGG 3 cut(s) 12, 144, 882
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 1 cut(s) 576
Bse3DI GCAATG 1 cut(s) 633
BseBI CCWGG 2 cut(s) 721, 884
BseDI CCNNGG 3 cut(s) 12, 144, 882
BseGI GGATG 2 cut(s) 99, 154
BseLI CCNNNNNNNGG 1 cut(s) 576
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 1 cut(s) 540
BseRI GAGGAG 1 cut(s) 233
BseXI GCAGC 3 cut(s) 552, 620, 887
BsgI GTGCAG 1 cut(s) 425
Bsh1236I CGCG 1 cut(s) 600
BsiHKAI GWGCWC 1 cut(s) 584
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 576
BsmFI GGGAC 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 584
Bsp1407I TGTACA 1 cut(s) 519
Bsp143I GATC 5 cut(s) 109, 269, 294, 601, 826
Bsp1720I GCTNAGC 1 cut(s) 549
Bsp19I CCATGG 1 cut(s) 12
Bsp68I TCGCGA 1 cut(s) 600
BspACI CCGC 5 cut(s) 193, 429, 616, 632, 853
BspCNI CTCAG 1 cut(s) 541
BspFNI CGCG 1 cut(s) 600
BspLI GGNNCC 1 cut(s) 725
BspPI GGATC 2 cut(s) 117, 302
BsrBI CCGCTC 1 cut(s) 616
BsrDI GCAATG 1 cut(s) 633
BsrGI TGTACA 1 cut(s) 519
BssECI CCNNGG 3 cut(s) 12, 144, 882
BssMI GATC 5 cut(s) 109, 269, 294, 601, 826
BssT1I CCWWGG 2 cut(s) 12, 144
Bst2UI CCWGG 2 cut(s) 721, 884
Bst4CI ACNGT 1 cut(s) 524
Bst6I CTCTTC 1 cut(s) 497
BstAPI GCANNNNNTGC 1 cut(s) 659
BstAUI TGTACA 1 cut(s) 519
BstBAI YACGTR 2 cut(s) 250, 478
BstC8I GCNNGC 1 cut(s) 534
BstDEI CTNAG 3 cut(s) 433, 549, 554
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 99, 154
BstFNI CGCG 1 cut(s) 600
BstKTI GATC 5 cut(s) 112, 272, 297, 604, 829
BstMBI GATC 5 cut(s) 109, 269, 294, 601, 826
BstMWI GCNNNNNNNGC 3 cut(s) 193, 434, 659
BstNI CCWGG 2 cut(s) 721, 884
BstSCI CCNGG 2 cut(s) 719, 882
BstSNI TACGTA 2 cut(s) 250, 478
BstUI CGCG 1 cut(s) 600
BstV1I GCAGC 3 cut(s) 552, 620, 887
BstV2I GAAGAC 2 cut(s) 268, 894
BstX2I RGATCY 2 cut(s) 109, 269
BstYI RGATCY 2 cut(s) 109, 269
BsuI GTATCC 1 cut(s) 266
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 2 cut(s) 99, 154
BtsI GCAGTG 2 cut(s) 135, 437
BtsIMutI CAGTG 2 cut(s) 135, 437
BtuMI TCGCGA 1 cut(s) 600
Cac8I GCNNGC 1 cut(s) 534
Cfr13I GGNCC 1 cut(s) 152
Csp6I GTAC 4 cut(s) 388, 520, 740, 811
CviAII CATG 2 cut(s) 13, 728
CviQI GTAC 4 cut(s) 388, 520, 740, 811
DdeI CTNAG 3 cut(s) 433, 549, 554
DpnI GATC 5 cut(s) 111, 271, 296, 603, 828
DpnII GATC 5 cut(s) 109, 269, 294, 601, 826
DraIII CACNNNGTG 1 cut(s) 422
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
EciI GGCGGA 1 cut(s) 647
Ecl136II GAGCTC 1 cut(s) 582
Eco105I TACGTA 2 cut(s) 250, 478
Eco130I CCWWGG 2 cut(s) 12, 144
Eco24I GRGCYC 1 cut(s) 584
Eco47I GGWCC 1 cut(s) 152
Eco53kI GAGCTC 1 cut(s) 582
Eco57I CTGAAG 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 582
EcoRII CCWGG 2 cut(s) 719, 882
EcoT14I CCWWGG 2 cut(s) 12, 144
EcoT38I GRGCYC 1 cut(s) 584
ErhI CCWWGG 2 cut(s) 12, 144
FaeI CATG 2 cut(s) 16, 731
FalI AAGNNNNNCTT 2 cut(s) 680, 712
FaqI GGGAC 1 cut(s) 30
FatI CATG 2 cut(s) 12, 727
Fnu4HI GCNGC 5 cut(s) 194, 429, 566, 609, 876
FokI GGATG 2 cut(s) 106, 161
FriOI GRGCYC 1 cut(s) 584
Fsp4HI GCNGC 5 cut(s) 194, 429, 566, 609, 876
FspBI CTAG 2 cut(s) 366, 830
GluI GCNGC 5 cut(s) 194, 429, 566, 609, 876
GsuI CTGGAG 1 cut(s) 598
Hin1II CATG 2 cut(s) 16, 731
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 2 cut(s) 221, 347
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 2 cut(s) 174, 842
Hpy188III TCNNGA 5 cut(s) 113, 577, 599, 764, 830
Hpy8I GTNNAC 1 cut(s) 355
HpyAV CCTTC 2 cut(s) 613, 852
HpyCH4III ACNGT 1 cut(s) 524
HpyCH4IV ACGT 2 cut(s) 249, 477
HpyCH4V TGCA 8 cut(s) 77, 335, 442, 449, 536, 608, 758, 808
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 434, 659
HpyF3I CTNAG 3 cut(s) 433, 549, 554
HpySE526I ACGT 2 cut(s) 249, 477
Hsp92II CATG 2 cut(s) 16, 731
Kzo9I GATC 5 cut(s) 109, 269, 294, 601, 826
LmnI GCTCC 2 cut(s) 579, 723
LpnPI CCDG 9 cut(s) 220, 530, 546, 562, 706, 722, 733, 869, 896
Lsp1109I GCAGC 3 cut(s) 552, 620, 887
LweI GCATC 1 cut(s) 322
MaeI CTAG 2 cut(s) 366, 830
MaeII ACGT 2 cut(s) 249, 477
MaeIII GTNAC 1 cut(s) 473
MalI GATC 5 cut(s) 111, 271, 296, 603, 828
MbiI CCGCTC 1 cut(s) 616
MboI GATC 5 cut(s) 109, 269, 294, 601, 826
MboII GAAGA 4 cut(s) 40, 268, 484, 899
MflI RGATCY 2 cut(s) 109, 269
MhlI GDGCHC 1 cut(s) 584
MluCI AATT 7 cut(s) 79, 156, 208, 282, 375, 465, 783
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 2 cut(s) 272, 378
MnlI CCTC 9 cut(s) 51, 211, 327, 498, 580, 684, 721, 758, 889
MspR9I CCNGG 2 cut(s) 721, 884
MvaI CCWGG 2 cut(s) 721, 884
MvnI CGCG 1 cut(s) 600
MwoI GCNNNNNNNGC 3 cut(s) 193, 434, 659
NcoI CCATGG 1 cut(s) 12
NdeII GATC 5 cut(s) 109, 269, 294, 601, 826
NlaIII CATG 2 cut(s) 16, 731
NlaIV GGNNCC 1 cut(s) 725
NruI TCGCGA 1 cut(s) 600
PfeI GAWTC 1 cut(s) 347
PfoI TCCNGGA 1 cut(s) 719
PkrI GCNGC 5 cut(s) 195, 430, 567, 610, 877
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
Ppu21I YACGTR 2 cut(s) 250, 478
Psp124BI GAGCTC 1 cut(s) 584
Psp6I CCWGG 2 cut(s) 719, 882
PspGI CCWGG 2 cut(s) 719, 882
PspN4I GGNNCC 1 cut(s) 725
PspPI GGNCC 1 cut(s) 152
PsrI GAACNNNNNNTAC 2 cut(s) 224, 256
PsuI RGATCY 2 cut(s) 109, 269
RruI TCGCGA 1 cut(s) 600
RsaI GTAC 4 cut(s) 389, 521, 741, 812
RsaNI GTAC 4 cut(s) 388, 520, 740, 811
SacI GAGCTC 1 cut(s) 584
SatI GCNGC 5 cut(s) 194, 429, 566, 609, 876
Sau3AI GATC 5 cut(s) 109, 269, 294, 601, 826
Sau96I GGNCC 1 cut(s) 152
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 2 cut(s) 721, 884
SduI GDGCHC 1 cut(s) 584
SfaNI GCATC 1 cut(s) 322
SinI GGWCC 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
SnaBI TACGTA 2 cut(s) 250, 478
Sse9I AATT 7 cut(s) 79, 156, 208, 282, 375, 465, 783
SsiI CCGC 5 cut(s) 193, 429, 616, 632, 853
SspI AATATT 2 cut(s) 454, 639
SspMI CTAG 2 cut(s) 366, 830
SstI GAGCTC 1 cut(s) 584
StyD4I CCNGG 2 cut(s) 719, 882
StyI CCWWGG 2 cut(s) 12, 144
TaaI ACNGT 1 cut(s) 524
TaiI ACGT 2 cut(s) 252, 480
TaqI TCGA 1 cut(s) 584
TasI AATT 7 cut(s) 79, 156, 208, 282, 375, 465, 783
TatI WGTACW 3 cut(s) 387, 519, 810
TauI GCSGC 2 cut(s) 196, 431
TfiI GAWTC 1 cut(s) 347
TscAI CASTG 2 cut(s) 142, 444
TseI GCWGC 3 cut(s) 565, 608, 875
TspDTI ATGAA 3 cut(s) 52, 110, 579
TspRI CASTG 2 cut(s) 142, 444
VpaK11BI GGWCC 1 cut(s) 152
XapI RAATTY 2 cut(s) 79, 208
XbaI TCTAGA 1 cut(s) 829
XspI CTAG 2 cut(s) 366, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.