Rw1G031030

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003956
Physical Location & Seq
Reverse (-)
59023442 .. 59024107
666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G031030.1

Sequence Viewer

Length: 666 bp
ATGGGAAAGTTGTGGTTTCAAAGGACTACAAAGAAGAAGAATAAGTTCTTCAAAAAAGAGAAGCAGCAGAAAGTAGTGATTGGAGTTTTGGCCTTCGAAGTTGCAAGAGTCATGTCTAGGCTGCTCCATCTATGGACATCTCTGAGCGATGAACATGTTAACAGGCTCAGACAACATATTTTAAATTCCGTGGGTATAGAAAAGCTTGTGTCAGATGACAATGAGTTCACACTAGGTTTAATATCCAGCGAATTGTTTGAGAATGTGGTCACTGTGGCAAATTATGTGGCCAGGCTAGGGAAGAATCATTCTTCTCATCCTAGTTTGAAGGCTTTTCAGCATGCTATTAGTGATTGGATCCATAATGGTGTTGACCCTTATGGATGGCAACTTTCATGGGAGAAGATGGAAAGGGAGTCCAACATGATGAAGGAGTTCATTTCAATAAATGCAGATTTGTATGACAAGATGAAACTTCTTTCGGATCTTGAACATACTTTGAAAGACATCACTGCCACCTTAGATGGTCCAATTTTGCTTGAGTTTCAGAACAAGGTAGAGCTGAAGCGGCACGAGGTGGAGAATTTGAAAGAGGTCTTGTTGGAGGTGAAAAATTCCGTAGAGGAGTTTGACCCAACAATTAATGCAGACTGGAGCGGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

221

Amino Acids

25.62

Weight (kDa)

6.32

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 29 - 84 8.4e-24 Domain of unknown function (DUF3475)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 657
AciI CCGC 2 cut(s) 568, 657
AclWI GGATC 3 cut(s) 352, 365, 492
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 3 cut(s) 184, 583, 613
AcuI CTGAAG 1 cut(s) 584
AdeI CACNNNGTG 1 cut(s) 577
AfiI CCNNNNNNNGG 1 cut(s) 297
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 7 cut(s) 20, 52, 328, 444, 491, 502, 589
AjnI CCWGG 1 cut(s) 290
AluBI AGCT 3 cut(s) 205, 562, 663
AluI AGCT 3 cut(s) 205, 562, 663
AlwI GGATC 3 cut(s) 352, 365, 492
AoxI GGCC 2 cut(s) 90, 288
ApeKI GCWGC 2 cut(s) 64, 121
ApoI RAATTY 3 cut(s) 184, 583, 613
AseI ATTAAT 1 cut(s) 642
Asp700I GAANNNNTTC 2 cut(s) 44, 434
AspS9I GGNCC 1 cut(s) 527
AsuHPI GGTGA 1 cut(s) 619
AsuII TTCGAA 1 cut(s) 96
AvaII GGWCC 1 cut(s) 527
BalI TGGCCA 1 cut(s) 290
BamHI GGATCC 1 cut(s) 357
BauI CACGAG 1 cut(s) 572
BbvI GCAGC 2 cut(s) 76, 108
BccI CCATC 4 cut(s) 135, 378, 400, 518
BciT130I CCWGG 1 cut(s) 292
BfaI CTAG 4 cut(s) 117, 233, 296, 321
BisI GCNGC 3 cut(s) 65, 122, 569
BlsI GCNGC 3 cut(s) 66, 123, 570
Bme1390I CCNGG 1 cut(s) 292
Bme18I GGWCC 1 cut(s) 527
BmgT120I GGNCC 1 cut(s) 527
BmiI GGNNCC 1 cut(s) 359
BmrFI CCNGG 1 cut(s) 292
Bpu14I TTCGAA 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 560
BsaJI CCNNGG 1 cut(s) 189
Bsc4I CCNNNNNNNGG 1 cut(s) 297
Bse1I ACTGG 1 cut(s) 656
BseBI CCWGG 1 cut(s) 292
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 2 cut(s) 316, 389
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMII CTCAG 2 cut(s) 134, 181
BseNI ACTGG 1 cut(s) 656
BseRI GAGGAG 1 cut(s) 638
BseXI GCAGC 2 cut(s) 76, 108
BshFI GGCC 2 cut(s) 92, 290
BslI CCNNNNNNNGG 1 cut(s) 297
BsnI GGCC 2 cut(s) 92, 290
Bsp119I TTCGAA 1 cut(s) 96
Bsp143I GATC 2 cut(s) 357, 484
BspACI CCGC 2 cut(s) 568, 657
BspANI GGCC 2 cut(s) 92, 290
BspCNI CTCAG 2 cut(s) 135, 180
BspLI GGNNCC 1 cut(s) 359
BspPI GGATC 3 cut(s) 352, 365, 492
BspT104I TTCGAA 1 cut(s) 96
BsrBI CCGCTC 1 cut(s) 657
BsrI ACTGG 1 cut(s) 656
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 2 cut(s) 357, 484
BssSI CACGAG 1 cut(s) 572
Bst2BI CACGAG 1 cut(s) 572
Bst2UI CCWGG 1 cut(s) 292
Bst4CI ACNGT 1 cut(s) 274
BstBI TTCGAA 1 cut(s) 96
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 3 cut(s) 143, 167, 520
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 2 cut(s) 316, 389
BstKTI GATC 2 cut(s) 360, 487
BstMBI GATC 2 cut(s) 357, 484
BstMWI GCNNNNNNNGC 1 cut(s) 568
BstNI CCWGG 1 cut(s) 292
BstNSI RCATGY 2 cut(s) 158, 344
BstSCI CCNGG 1 cut(s) 290
BstV1I GCAGC 2 cut(s) 76, 108
BstX2I RGATCY 2 cut(s) 357, 484
BstYI RGATCY 2 cut(s) 357, 484
BsuRI GGCC 2 cut(s) 92, 290
BtgI CCRYGG 1 cut(s) 189
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 2 cut(s) 316, 389
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 2 cut(s) 270, 510
Cac8I GCNNGC 1 cut(s) 342
Cfr13I GGNCC 1 cut(s) 527
CspCI CAANNNNNGTGG 2 cut(s) 267, 302
CviAII CATG 5 cut(s) 112, 155, 341, 396, 424
CviJI RGCY 9 cut(s) 92, 121, 166, 205, 290, 295, 332, 562, 663
CviKI_1 RGCY 9 cut(s) 92, 121, 166, 205, 290, 295, 332, 562, 663
DdeI CTNAG 3 cut(s) 143, 167, 520
DpnI GATC 2 cut(s) 359, 486
DpnII GATC 2 cut(s) 357, 484
DraI TTTAAA 1 cut(s) 183
DraIII CACNNNGTG 1 cut(s) 577
EaeI YGGCCR 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 527
Eco57I CTGAAG 1 cut(s) 584
EcoRII CCWGG 1 cut(s) 290
FaeI CATG 5 cut(s) 115, 158, 344, 399, 427
FatI CATG 5 cut(s) 111, 154, 340, 395, 423
FauI CCCGC 1 cut(s) 650
Fnu4HI GCNGC 3 cut(s) 65, 122, 569
FokI GGATG 2 cut(s) 303, 396
Fsp4HI GCNGC 3 cut(s) 65, 122, 569
FspBI CTAG 4 cut(s) 117, 233, 296, 321
GluI GCNGC 3 cut(s) 65, 122, 569
HaeIII GGCC 2 cut(s) 92, 290
Hin1II CATG 5 cut(s) 115, 158, 344, 399, 427
HincII GTYRAC 2 cut(s) 160, 373
HindII GTYRAC 2 cut(s) 160, 373
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 3 cut(s) 108, 304, 416
HpaI GTTAAC 1 cut(s) 160
HphI GGTGA 1 cut(s) 619
Hpy166II GTNNAC 3 cut(s) 160, 228, 373
Hpy188I TCNGA 5 cut(s) 144, 170, 214, 484, 549
Hpy188III TCNNGA 1 cut(s) 488
Hpy8I GTNNAC 3 cut(s) 160, 228, 373
HpyAV CCTTC 3 cut(s) 103, 322, 424
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4V TGCA 3 cut(s) 104, 452, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 568
HpyF3I CTNAG 3 cut(s) 143, 167, 520
Hsp92II CATG 5 cut(s) 115, 158, 344, 399, 427
KspAI GTTAAC 1 cut(s) 160
Kzo9I GATC 2 cut(s) 357, 484
LmnI GCTCC 3 cut(s) 129, 654, 660
LpnPI CCDG 5 cut(s) 148, 259, 277, 304, 637
Lsp1109I GCAGC 2 cut(s) 76, 108
MaeI CTAG 4 cut(s) 117, 233, 296, 321
MaeIII GTNAC 1 cut(s) 268
MalI GATC 2 cut(s) 359, 486
MbiI CCGCTC 1 cut(s) 657
MboI GATC 2 cut(s) 357, 484
MboII GAAGA 6 cut(s) 40, 46, 49, 303, 313, 415
MflI RGATCY 2 cut(s) 357, 484
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 7 cut(s) 184, 251, 280, 531, 583, 613, 639
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 2 cut(s) 117, 425
MmeI TCCRAC 2 cut(s) 444, 582
MnlI CCTC 4 cut(s) 568, 586, 598, 616
Mox20I TGGCCA 1 cut(s) 290
MroXI GAANNNNTTC 2 cut(s) 44, 434
MscI TGGCCA 1 cut(s) 290
MseI TTAA 4 cut(s) 159, 182, 239, 642
MslI CAYNNNNRTG 1 cut(s) 366
Msp20I TGGCCA 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 292
MvaI CCWGG 1 cut(s) 292
MwoI GCNNNNNNNGC 1 cut(s) 568
NdeII GATC 2 cut(s) 357, 484
NlaIII CATG 5 cut(s) 115, 158, 344, 399, 427
NlaIV GGNNCC 1 cut(s) 359
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 2 cut(s) 158, 344
NspV TTCGAA 1 cut(s) 96
PaeI GCATGC 1 cut(s) 344
PciI ACATGT 1 cut(s) 154
PdmI GAANNNNTTC 2 cut(s) 44, 434
PfeI GAWTC 1 cut(s) 304
PkrI GCNGC 3 cut(s) 66, 123, 570
PleI GAGTC 2 cut(s) 116, 424
PpsI GAGTC 2 cut(s) 116, 424
PscI ACATGT 1 cut(s) 154
PshBI ATTAAT 1 cut(s) 642
Psp6I CCWGG 1 cut(s) 290
PspGI CCWGG 1 cut(s) 290
PspN4I GGNNCC 1 cut(s) 359
PspPI GGNCC 1 cut(s) 527
PsuI RGATCY 2 cut(s) 357, 484
RseI CAYNNNNRTG 1 cut(s) 366
SaqAI TTAA 4 cut(s) 159, 182, 239, 642
SatI GCNGC 3 cut(s) 65, 122, 569
Sau3AI GATC 2 cut(s) 357, 484
Sau96I GGNCC 1 cut(s) 527
SchI GAGTC 2 cut(s) 117, 425
ScrFI CCNGG 1 cut(s) 292
SetI ASST 9 cut(s) 207, 238, 521, 558, 564, 579, 597, 609, 665
SfuI TTCGAA 1 cut(s) 96
SinI GGWCC 1 cut(s) 527
SmiMI CAYNNNNRTG 1 cut(s) 366
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
SphI GCATGC 1 cut(s) 344
Sse9I AATT 7 cut(s) 184, 251, 280, 531, 583, 613, 639
SsiI CCGC 2 cut(s) 568, 657
SspMI CTAG 4 cut(s) 117, 233, 296, 321
StyD4I CCNGG 1 cut(s) 290
TaaI ACNGT 1 cut(s) 274
TaqI TCGA 1 cut(s) 96
TasI AATT 7 cut(s) 184, 251, 280, 531, 583, 613, 639
TauI GCSGC 1 cut(s) 571
TfiI GAWTC 1 cut(s) 304
Tru1I TTAA 4 cut(s) 159, 182, 239, 642
Tru9I TTAA 4 cut(s) 159, 182, 239, 642
TscAI CASTG 2 cut(s) 277, 517
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 2 cut(s) 64, 121
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 5 cut(s) 165, 384, 427, 443, 485
TspGWI ACGGA 2 cut(s) 178, 607
TspRI CASTG 2 cut(s) 277, 517
VpaK11BI GGWCC 1 cut(s) 527
VspI ATTAAT 1 cut(s) 642
XapI RAATTY 3 cut(s) 184, 583, 613
XceI RCATGY 2 cut(s) 158, 344
XmnI GAANNNNTTC 2 cut(s) 44, 434
XspI CTAG 4 cut(s) 117, 233, 296, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.