RchiOBHm_Chr1g0370531

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006852
Physical Location & Seq
Forward (+)
60034940 .. 60039030
4091 bp
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UTR
Exon/CDS
Intron
PRQ59469

Sequence Viewer

Length: 1050 bp
ATGGTACTTGGTGAGAAGTTGCAAGAAGCGGATAAAGAAATAGAAAATAAATGGGTATATGTGGATGGTAAAAAGATGGAGAATGGAATGTTTAAAAGACAAATCAACCCAAGTGATGTTATCATCAACACTGGAGATACAAGTCATACTCCACGCTGCCCCATTCCTGGACGTTGGAAGAAAGTTGTACGCGATCCTCATCTTAAATGGCTCGCATATTGGAAAGATTCAATTTTCGGGAGAAGAAAGTACATGAACTTGAAATATTGTAGCAGTATGTTTGACATTTCAAAATTTAACACCGCGAGGGAACTAAAGATCAATCAAGCAGAGATCAAGCAACAATACACAGTGGATTTCACCCGTTCAAATCTTATGGATAAGCAATGTGCAATTGTTACATATCTTATTGATACATTAGCTTTAAGACCTGGACATGTTAAGGACGGAGAAATTACGGCACCTACGGTTGGATGCTGCACTTTAATGGTGAAAAATGTAATTTTGCATGAACCCTCATGTTTGGAGTTTCGCTTTACTGGGAAAGCATTAGTCGACTACGAGAATGAGGTTGAAGTTGAAAACCTTTTGAACAAGGAAGACTTGAGGGAGAAAACAGAAGAAGATAAGCTTGTTGTTTATTGGAATTCAAACAAGGAAGTTGCTATTTTTCTAAATCATCGGAGAGAAACGTTGGATTTTGAAGAGGAACAAGCTCGTAAGCTTAGCATACAGATTTGTGAACTAAATGCAAGTATTGACCAAAAATCAGAACTGGAGATTCAAAGAACTCATCCAAGTGATAAGTATTATGCCTCTGAAGCGGGAAAACTTGAGTTAGACATTGAAATAGAGAAGATAATGGAGAAGATTCATGAGCTGAGTCTACAAGAGGGTACCATAGCCTGTAGTCAAACTTATCGATTAGAAACATTGAGAGAGAATTACCTTGATCCCAGGATTACTGTTGCATGGTGTCTACGACATAGGATTTCAGTTTCTAAGGTGAGCATTTCTTTTGTTGCCGTTGGATATGGGAAAGAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

349

Amino Acids

40.63

Weight (kDa)

6.14

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I_N PF02919 23 - 86 2e-14 Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I PF01028 94 - 195 4.4e-27 Eukaryotic DNA topoisomerase I, catalytic core
Topo_C_assoc PF14370 289 - 336 4.4e-08 C-terminal topoisomerase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 896
AccB1I GGYRCC 2 cut(s) 460, 896
AccI GTMKAC 3 cut(s) 555, 886, 979
AccII CGCG 2 cut(s) 192, 305
AciI CCGC 3 cut(s) 29, 303, 824
AclI AACGTT 1 cut(s) 692
AclWI GGATC 2 cut(s) 188, 947
AcsI RAATTY 2 cut(s) 293, 646
AcuI CTGAAG 1 cut(s) 840
AfaI GTAC 4 cut(s) 6, 189, 251, 898
AfiI CCNNNNNNNGG 2 cut(s) 167, 470
AflIII ACRYGT 1 cut(s) 436
AjnI CCWGG 3 cut(s) 166, 430, 956
AluBI AGCT 5 cut(s) 422, 631, 716, 724, 880
AluI AGCT 5 cut(s) 422, 631, 716, 724, 880
AlwI GGATC 2 cut(s) 188, 947
ApeKI GCWGC 2 cut(s) 156, 477
ApoI RAATTY 2 cut(s) 293, 646
Asp718I GGTACC 1 cut(s) 896
AsuHPI GGTGA 4 cut(s) 23, 352, 502, 1018
BanI GGYRCC 2 cut(s) 460, 896
BbsI GAAGAC 1 cut(s) 606
BbvI GCAGC 2 cut(s) 143, 464
BccI CCATC 2 cut(s) 59, 70
BceAI ACGGC 2 cut(s) 474, 1010
BciT130I CCWGG 3 cut(s) 168, 432, 958
BfmI CTRYAG 1 cut(s) 907
BisI GCNGC 2 cut(s) 157, 478
BlpI GCTNAGC 1 cut(s) 725
BlsI GCNGC 2 cut(s) 158, 479
Bme1390I CCNGG 3 cut(s) 168, 432, 958
BmiI GGNNCC 2 cut(s) 462, 898
BmrFI CCNGG 3 cut(s) 168, 432, 958
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 1 cut(s) 464
BmuI ACTGGG 1 cut(s) 549
BpiI GAAGAC 1 cut(s) 606
BpmI CTGGAG 2 cut(s) 153, 797
Bpu1102I GCTNAGC 1 cut(s) 725
BpuEI CTTGAG 2 cut(s) 625, 854
Bsa29I ATCGAT 1 cut(s) 922
BsaBI GATNNNNATC 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 956
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 470
Bse1I ACTGG 3 cut(s) 136, 544, 780
Bse3DI GCAATG 1 cut(s) 392
Bse8I GATNNNNATC 1 cut(s) 198
BseBI CCWGG 3 cut(s) 168, 432, 958
BseCI ATCGAT 1 cut(s) 922
BseDI CCNNGG 1 cut(s) 956
BseGI GGATG 3 cut(s) 70, 479, 793
BseJI GATNNNNATC 1 cut(s) 198
BseLI CCNNNNNNNGG 2 cut(s) 167, 470
BseMI GCAATG 1 cut(s) 392
BseMII CTCAG 1 cut(s) 872
BseNI ACTGG 3 cut(s) 136, 544, 780
BseXI GCAGC 2 cut(s) 143, 464
BsgI GTGCAG 1 cut(s) 463
Bsh1236I CGCG 2 cut(s) 192, 305
BshNI GGYRCC 2 cut(s) 460, 896
BshVI ATCGAT 1 cut(s) 922
BslI CCNNNNNNNGG 2 cut(s) 167, 470
Bsp143I GATC 4 cut(s) 193, 318, 333, 952
Bsp1720I GCTNAGC 1 cut(s) 725
BspACI CCGC 3 cut(s) 29, 303, 824
BspCNI CTCAG 1 cut(s) 873
BspDI ATCGAT 1 cut(s) 922
BspFNI CGCG 2 cut(s) 192, 305
BspHI TCATGA 1 cut(s) 874
BspLI GGNNCC 2 cut(s) 462, 898
BspPI GGATC 2 cut(s) 188, 947
BspT107I GGYRCC 2 cut(s) 460, 896
BsrDI GCAATG 1 cut(s) 392
BsrI ACTGG 3 cut(s) 136, 544, 780
BssECI CCNNGG 1 cut(s) 956
BssMI GATC 4 cut(s) 193, 318, 333, 952
Bst2UI CCWGG 3 cut(s) 168, 432, 958
Bst4CI ACNGT 3 cut(s) 352, 469, 967
Bst6I CTCTTC 1 cut(s) 699
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 3 cut(s) 725, 881, 1002
BstF5I GGATG 3 cut(s) 70, 479, 793
BstFNI CGCG 2 cut(s) 192, 305
BstKTI GATC 4 cut(s) 196, 321, 336, 955
BstMBI GATC 4 cut(s) 193, 318, 333, 952
BstMWI GCNNNNNNNGC 1 cut(s) 821
BstNI CCWGG 3 cut(s) 168, 432, 958
BstNSI RCATGY 1 cut(s) 440
BstSCI CCNGG 3 cut(s) 166, 430, 956
BstSFI CTRYAG 1 cut(s) 907
BstUI CGCG 2 cut(s) 192, 305
BstV1I GCAGC 2 cut(s) 143, 464
BstV2I GAAGAC 1 cut(s) 606
Bsu15I ATCGAT 1 cut(s) 922
BsuTUI ATCGAT 1 cut(s) 922
BtsCI GGATG 3 cut(s) 70, 479, 793
BtsIMutI CAGTG 2 cut(s) 129, 357
Cac8I GCNNGC 1 cut(s) 213
CciI TCATGA 1 cut(s) 874
ClaI ATCGAT 1 cut(s) 922
Csp6I GTAC 4 cut(s) 5, 188, 250, 897
CviAII CATG 6 cut(s) 253, 437, 509, 519, 875, 972
CviJI RGCY 7 cut(s) 211, 422, 631, 716, 724, 880, 905
CviKI_1 RGCY 7 cut(s) 211, 422, 631, 716, 724, 880, 905
CviQI GTAC 4 cut(s) 5, 188, 250, 897
DdeI CTNAG 3 cut(s) 725, 881, 1002
DpnI GATC 4 cut(s) 195, 320, 335, 954
DpnII GATC 4 cut(s) 193, 318, 333, 952
DraI TTTAAA 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 699
EarI CTCTTC 1 cut(s) 699
Eco57I CTGAAG 1 cut(s) 840
EcoRI GAATTC 1 cut(s) 646
EcoRII CCWGG 3 cut(s) 166, 430, 956
FaeI CATG 6 cut(s) 256, 440, 512, 522, 878, 975
FalI AAGNNNNNCTT 4 cut(s) 587, 619, 615, 647
FatI CATG 6 cut(s) 252, 436, 508, 518, 874, 971
FauI CCCGC 1 cut(s) 817
FblI GTMKAC 3 cut(s) 555, 886, 979
Fnu4HI GCNGC 2 cut(s) 157, 478
FokI GGATG 3 cut(s) 77, 486, 780
Fsp4HI GCNGC 2 cut(s) 157, 478
GluI GCNGC 2 cut(s) 157, 478
GsuI CTGGAG 2 cut(s) 153, 797
Hin1II CATG 6 cut(s) 256, 440, 512, 522, 878, 975
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HindIII AAGCTT 2 cut(s) 629, 722
HinfI GANTC 4 cut(s) 227, 781, 871, 883
HphI GGTGA 4 cut(s) 23, 352, 502, 1018
Hpy166II GTNNAC 4 cut(s) 556, 743, 887, 980
Hpy188I TCNGA 3 cut(s) 684, 772, 820
Hpy188III TCNNGA 2 cut(s) 238, 875
Hpy8I GTNNAC 4 cut(s) 556, 743, 887, 980
HpyCH4III ACNGT 3 cut(s) 352, 469, 967
HpyCH4IV ACGT 2 cut(s) 172, 692
HpyCH4V TGCA 6 cut(s) 22, 392, 480, 508, 752, 971
HpyF10VI GCNNNNNNNGC 1 cut(s) 821
HpyF3I CTNAG 3 cut(s) 725, 881, 1002
HpySE526I ACGT 2 cut(s) 172, 692
Hsp92II CATG 6 cut(s) 256, 440, 512, 522, 878, 975
KpnI GGTACC 1 cut(s) 900
Kzo9I GATC 4 cut(s) 193, 318, 333, 952
Lsp1109I GCAGC 2 cut(s) 143, 464
LweI GCATC 1 cut(s) 464
MaeII ACGT 2 cut(s) 172, 692
MaeIII GTNAC 1 cut(s) 397
MalI GATC 4 cut(s) 195, 320, 335, 954
MboI GATC 4 cut(s) 193, 318, 333, 952
MboII GAAGA 8 cut(s) 190, 255, 611, 632, 635, 716, 868, 880
MfeI CAATTG 1 cut(s) 393
MluCI AATT 7 cut(s) 231, 293, 393, 453, 501, 646, 943
MlyI GAGTC 1 cut(s) 892
MmeI TCCRAC 4 cut(s) 155, 451, 675, 1009
MnlI CCTC 8 cut(s) 207, 300, 526, 562, 600, 700, 826, 886
MseI TTAA 6 cut(s) 93, 204, 297, 425, 441, 485
MslI CAYNNNNRTG 2 cut(s) 485, 798
MspR9I CCNGG 3 cut(s) 168, 432, 958
MunI CAATTG 1 cut(s) 393
MvaI CCWGG 3 cut(s) 168, 432, 958
MvnI CGCG 2 cut(s) 192, 305
MwoI GCNNNNNNNGC 1 cut(s) 821
NdeII GATC 4 cut(s) 193, 318, 333, 952
NlaIII CATG 6 cut(s) 256, 440, 512, 522, 878, 975
NlaIV GGNNCC 2 cut(s) 462, 898
NspI RCATGY 1 cut(s) 440
PagI TCATGA 1 cut(s) 874
PciI ACATGT 1 cut(s) 436
PfeI GAWTC 3 cut(s) 227, 781, 871
PfoI TCCNGGA 1 cut(s) 166
PkrI GCNGC 2 cut(s) 158, 479
PleI GAGTC 1 cut(s) 891
PpsI GAGTC 1 cut(s) 891
PscI ACATGT 1 cut(s) 436
Psp1406I AACGTT 1 cut(s) 692
Psp6I CCWGG 3 cut(s) 166, 430, 956
PspGI CCWGG 3 cut(s) 166, 430, 956
PspN4I GGNNCC 2 cut(s) 462, 898
RsaI GTAC 4 cut(s) 6, 189, 251, 898
RsaNI GTAC 4 cut(s) 5, 188, 250, 897
RseI CAYNNNNRTG 2 cut(s) 485, 798
SalI GTCGAC 1 cut(s) 554
SaqAI TTAA 6 cut(s) 93, 204, 297, 425, 441, 485
SatI GCNGC 2 cut(s) 157, 478
Sau3AI GATC 4 cut(s) 193, 318, 333, 952
SchI GAGTC 1 cut(s) 892
ScrFI CCNGG 3 cut(s) 168, 432, 958
SfaNI GCATC 1 cut(s) 464
SfcI CTRYAG 1 cut(s) 907
SmiMI CAYNNNNRTG 2 cut(s) 485, 798
SmlI CTYRAG 2 cut(s) 604, 833
SmoI CTYRAG 2 cut(s) 604, 833
Sse9I AATT 7 cut(s) 231, 293, 393, 453, 501, 646, 943
SsiI CCGC 3 cut(s) 29, 303, 824
SspI AATATT 1 cut(s) 266
StyD4I CCNGG 3 cut(s) 166, 430, 956
TaaI ACNGT 3 cut(s) 352, 469, 967
TaiI ACGT 2 cut(s) 175, 695
TaqI TCGA 2 cut(s) 555, 922
TasI AATT 7 cut(s) 231, 293, 393, 453, 501, 646, 943
TatI WGTACW 1 cut(s) 249
TfiI GAWTC 3 cut(s) 227, 781, 871
Tru1I TTAA 6 cut(s) 93, 204, 297, 425, 441, 485
Tru9I TTAA 6 cut(s) 93, 204, 297, 425, 441, 485
TscAI CASTG 2 cut(s) 136, 357
TseI GCWGC 2 cut(s) 156, 477
TspDTI ATGAA 3 cut(s) 269, 525, 863
TspGWI ACGGA 1 cut(s) 462
TspRI CASTG 2 cut(s) 136, 357
XapI RAATTY 2 cut(s) 293, 646
XceI RCATGY 1 cut(s) 440
XmiI GTMKAC 3 cut(s) 555, 886, 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.