Rh1BG329800

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0006852
Physical Location & Seq
Forward (+)
46620436 .. 46694850
74415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG329800.1

Sequence Viewer

Length: 3063 bp
ATGGAGCTTTCGGTGAAAATTGAAGATGTAGAGTTGACTTCAGATGATGAGGATGATTATGCATCCGCAGATGAAGATGAGTGTAAAATTAAAGTCAAAAGGACAAAGATTACTGATAATTATGCGATATGGGCCACTTTATCTCATAAGGGTCCTATTTTTCCAACTCCATACAAGCCTCATGGGATAAAAGTAAAATATAATGGAGTAGAAGTAGACTTAAATCCTGAACAAGAGGAGCTTGCAACAATGTACGCAGAGTATAGACAAACTCATTTCGGTCATCGTGAGAAGACATTTCAAGAAAATTTTTGGAAAGAATGGCAAGAAATGCTTGGAGGAGAGCATGTAATCAAGGACCTAAGCAGTTGTGACTTTGGAGATATAGAGACATGGCTTGATGATGAGAAGAAGAGGAAAAAAGACATGAAGGACAAAGAGAAGTTGCAAGAAGCGGATGAAGAAATAGAAAATAAATGGGCATATGTGGATGGCAAAAAGATGGAGGTGTCATGCTATCAAATTGAACCACCTGGATTATTTATAGGCCGTGGCAAGCACCCAAAGAATGGAATGTTAAAAAGACGGATCAACCCAAGTGATGTTGTCATCAACATTGGAGATACAAGTCAGACTCCATGTTGTCCAATTCCTGGATGTTGGAAGAAAGTTGTACGCGATCCTCATGTTAAATGGCTCGCTTATTGGAAAGATTCAATTTCCGGGAGAAGAAAGTTCATGAACTTGAAATATTGCAGCAGTAAGTCTGACATTTCAAAATTTAACACCGCGAGGGAATTAAAGATTAATCAAGCAGAGATCAAGCGACAGTATACAGTGGATTTCACCAGTCTAAATCTTGTGGATAAACAATGTGCAATTGTTACTTATCTTATTGATACATTAGCTTTAAGACCTGGACATGTTAAGGACGAAGAAATTACTGCACCTACTGTTGGATGTTGCACGTTAATGGTGAAAAATGTAATTTTACATGAACCCTCATGTTTGGAGTTTCGCTTTACTGGGAAAGCATCAGTCGACTACGAGAATCAGGTTGAAGTTGAAAACCTTGTGTTTAATTGCATTAAAGAATTCCAGCTTGGAAAATCTCCTGAAGACAATTTGTTTGACAAGTTGGATGCTAAAAAATTGAATGGATATTTACAAAACTTGGGAAAACCTTTTGGCCTCCATTTTACAGCAAAGACATTCCGCACATTTCGAGCATCTGAAATGATGGATGATTTGTTGAACAAGGAAGACTTGAAGGAGAAAACAGAAGAAGATAAGCTTGCTGTTTACTGGAATGCAAACAAGGAAGTTGCTATTTTTCTAAATCATCGGAGAGAAGTGTCCGATTTTGAAGAGAAACAAGCTTGGGAGCTTAGCATACAGATCTTTGAACTAGAGGCAAACAATGAAATAGAGAAGATAATGAAGAAGATTCATGAGCTGAGTCTACAAGAGCGTACCATAGCATGTAGTCAAACTTATCGATTAGAAACATCTAGAGAGAATTACCTTGATCCCAGAATTACTGTTGCATGGTGTCTACAACATAGGATTTCAGTTTCTAAGATGTGTGCAAGAGGTACAGATGTTTTGTGGCTTCCCCAGATATTTGGGATCTGTTTGCGACCTACCTTGCTGAGTCTAAGGTGCCTCAGGATGAAGATACACAGACTTCAGAAAGCAGCTGTATTTCTTGATAACAACACATATCTGCATTTTTTTCACCTACATATTGATATTCCTGAGTCTCCTATGCTTGCAAATACTATTATACTCAGAACGTTCTGGATTGAGCGACCAGCAATAATGATTTTGGAACTCCATTGTACTGATCAAAATCCAAACAGTTTTTTCATAGCCAAGGGTCTATTTTTTCCTACAAACAGTACTGTAACAAGTCCTATACACCAGCATTTATCAATAAGGGCCTCATATCTAAAGCTTACACTCCTAAACTCAGAGAAGCTGTTGAGGAATGGAATGTTAAAAAGACGAATCAACCCAAGTGATGTTATCATCAACATTGAAGATACAAGTCAGACTCCACGCTACCCCATTCCTGGATGTAGGAAGAAAGTTGTACGCGATCCTCATCTTAAATGGCTCGCATATTGGAAATATTCAATTTCCGGGAGAAGAAAGTACATGAACTTGAAATATTGCAGAAGTATGTTTGACATTTCAAAATTTAACACCGCGAGGGAACTAAAGATCAATCAAGCAGAGATCAAGCAACAATACACAGTGGATTTCGCCAGTCCAAATCTTGTGGATAAGCAATGTGCAATTGTTACATATCTTATTGATACATTAGCTTTAAGACCTGGACATGTTAAGGACGAAGAAATTACTGCACCTACCGTTGGATGTTGCACATTAATGGTGAAAAATGTAATTTTACATGAACCCTCATGTTTGGAGTTTCGCTTTACTGGGAAAGCATACTATGAGAATGAGGTTGAAGTTGAAAACCTTGTGTTTAATTGCATTAAAGAATTCCAGCTTGGGAAATCTCCTGAAGACAATTTGTTTGACAAGTTGGATGCCAAAAAATTGAATAGATATTTACAAAACTTGGGAAAACCTTTTGGCCTCCATTTTACAGCAAAGACATTCCGCACATTTCGAGCATCTGAAATGATGGATGATTTGTTGAACAAGGAAGACTTGAAGGAGAAAACAGAAGAAGATAAGCTTGCTGTTTACTGGAATGCAAACAAGGAAGTTGCTATTTTTCTAAATCATCGGAGAGAAGTGTCCGATTTTGAAGAGAAACAAGGTTGGGAGCTTAGCATACAGATCTTTGAACTAGAGGCAAACAATGAAATAGAGAAGATAATGAAGAAGATTCATGAGCTGAGTCTACAAGAGCGTACCATAGCATGTAGTCAAACTTATCGATTAGAAACATCTAGAGAGAATTACCTTGATCCCAGAATTACTGTTGCATGGTGTCTACAACATAGGATTTCAGTTTCTAAGGTGAGCATTTCTTTTGTTGCCGTTGGATATGGGAAGTGCAAGTTGGTAGTCATACAGTTAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

1020

Amino Acids

118.87

Weight (kDa)

7.12

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I_N PF02919 44 - 248 3.7e-67 Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I PF01028 254 - 475 2.3e-59 Eukaryotic DNA topoisomerase I, catalytic core
Topo_C_assoc PF14370 481 - 528 2e-09 C-terminal topoisomerase domain
Topoisom_I_N PF02919 663 - 722 2e-11 Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I PF01028 727 - 946 1.7e-55 Eukaryotic DNA topoisomerase I, catalytic core
Topo_C_assoc PF14370 953 - 1000 2.4e-09 C-terminal topoisomerase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1662
AccB7I CCANNNNNTGG 2 cut(s) 569, 653
AccI GTMKAC 7 cut(s) 216, 833, 1041, 1462, 1555, 2878, 2971
AccII CGCG 4 cut(s) 678, 791, 2100, 2213
AciI CCGC 6 cut(s) 66, 455, 789, 1216, 2211, 2632
AclI AACGTT 1 cut(s) 1796
AclWI GGATC 6 cut(s) 596, 674, 1523, 1637, 2096, 2939
AcsI RAATTY 5 cut(s) 307, 779, 1094, 2201, 2510
AcuI CTGAAG 4 cut(s) 24, 1137, 1673, 2553
AfaI GTAC 9 cut(s) 254, 675, 1474, 1597, 1843, 1903, 2097, 2159, 2890
AfiI CCNNNNNNNGG 5 cut(s) 569, 653, 956, 2075, 2378
AflIII ACRYGT 2 cut(s) 922, 2344
AjnI CCWGG 5 cut(s) 532, 652, 916, 2074, 2338
AjuI GAANNNNNNNTTGG 6 cut(s) 1086, 1118, 2502, 2534, 3023, 3055
Alw26I GTCTC 2 cut(s) 383, 1767
AlwI GGATC 6 cut(s) 596, 674, 1523, 1637, 2096, 2939
AoxI GGCC 5 cut(s) 132, 547, 1189, 1941, 2605
ApeKI GCWGC 2 cut(s) 756, 1697
ApoI RAATTY 5 cut(s) 307, 779, 1094, 2201, 2510
ArsI GACNNNNNNTTYG 4 cut(s) 1112, 1144, 2528, 2560
AseI ATTAAT 2 cut(s) 807, 2393
AspS9I GGNCC 4 cut(s) 132, 152, 358, 1941
AsuC2I CCSGG 2 cut(s) 724, 2146
AsuHPI GGTGA 6 cut(s) 25, 838, 988, 1730, 2410, 3010
AvaII GGWCC 2 cut(s) 152, 358
AxyI CCTNAGG 1 cut(s) 1667
BaeI ACNNNNGTAYC 2 cut(s) 1587, 1620
BanI GGYRCC 1 cut(s) 1662
BbsI GAAGAC 5 cut(s) 299, 1125, 1269, 2541, 2685
BbvI GCAGC 2 cut(s) 768, 1709
BccI CCATC 4 cut(s) 485, 496, 1234, 2650
BceAI ACGGC 2 cut(s) 534, 3002
BciT130I CCWGG 5 cut(s) 534, 654, 918, 2076, 2340
BclI TGATCA 1 cut(s) 1846
BcnI CCSGG 2 cut(s) 724, 2146
BcoDI GTCTC 2 cut(s) 383, 1767
BfaI CTAG 4 cut(s) 1409, 1512, 2825, 2928
BglII AGATCT 2 cut(s) 1398, 2814
BisI GCNGC 2 cut(s) 757, 1698
BlpI GCTNAGC 2 cut(s) 1388, 2804
BlsI GCNGC 2 cut(s) 758, 1699
BmcAI AGTACT 1 cut(s) 1903
Bme1390I CCNGG 7 cut(s) 534, 654, 724, 918, 2076, 2146, 2340
Bme18I GGWCC 2 cut(s) 152, 358
BmgT120I GGNCC 4 cut(s) 132, 152, 358, 1941
BmiI GGNNCC 2 cut(s) 153, 1664
BmrFI CCNGG 7 cut(s) 534, 654, 724, 918, 2076, 2146, 2340
BmrI ACTGGG 2 cut(s) 1035, 2457
BmsI GCATC 6 cut(s) 71, 1043, 1132, 1238, 2548, 2654
BmuI ACTGGG 2 cut(s) 1035, 2457
BpiI GAAGAC 5 cut(s) 299, 1125, 1269, 2541, 2685
Bpu10I CCTNAGC 1 cut(s) 362
Bpu1102I GCTNAGC 2 cut(s) 1388, 2804
BpuMI CCSGG 2 cut(s) 724, 2146
Bsa29I ATCGAT 2 cut(s) 1498, 2914
BsaBI GATNNNNATC 2 cut(s) 1851, 2106
BsaJI CCNNGG 2 cut(s) 550, 1875
BsaXI ACNNNNNCTCC 4 cut(s) 198, 228, 333, 363
Bsc4I CCNNNNNNNGG 5 cut(s) 569, 653, 956, 2075, 2378
Bse1I ACTGG 6 cut(s) 849, 1030, 1310, 2271, 2452, 2726
Bse21I CCTNAGG 1 cut(s) 1667
Bse3DI GCAATG 1 cut(s) 2300
Bse8I GATNNNNATC 2 cut(s) 1851, 2106
BseBI CCWGG 5 cut(s) 534, 654, 918, 2076, 2340
BseCI ATCGAT 2 cut(s) 1498, 2914
BseDI CCNNGG 2 cut(s) 550, 1875
BseJI GATNNNNATC 2 cut(s) 1851, 2106
BseLI CCNNNNNNNGG 5 cut(s) 569, 653, 956, 2075, 2378
BseMI GCAATG 1 cut(s) 2300
BseMII CTCAG 7 cut(s) 1448, 1643, 1681, 1749, 1804, 1986, 2864
BseNI ACTGG 6 cut(s) 849, 1030, 1310, 2271, 2452, 2726
BseRI GAGGAG 2 cut(s) 251, 354
BseXI GCAGC 2 cut(s) 768, 1709
BsgI GTGCAG 2 cut(s) 930, 2352
Bsh1236I CGCG 4 cut(s) 678, 791, 2100, 2213
BshFI GGCC 5 cut(s) 134, 549, 1191, 1943, 2607
BshNI GGYRCC 1 cut(s) 1662
BshVI ATCGAT 2 cut(s) 1498, 2914
BsiSI CCGG 2 cut(s) 723, 2145
BslI CCNNNNNNNGG 5 cut(s) 569, 653, 956, 2075, 2378
BsmAI GTCTC 2 cut(s) 383, 1767
BsmI GAATGC 2 cut(s) 1315, 2731
BsnI GGCC 5 cut(s) 134, 549, 1191, 1943, 2607
Bsp1720I GCTNAGC 2 cut(s) 1388, 2804
BspACI CCGC 6 cut(s) 66, 455, 789, 1216, 2211, 2632
BspANI GGCC 5 cut(s) 134, 549, 1191, 1943, 2607
BspCNI CTCAG 7 cut(s) 1449, 1644, 1680, 1750, 1803, 1985, 2865
BspDI ATCGAT 2 cut(s) 1498, 2914
BspFNI CGCG 4 cut(s) 678, 791, 2100, 2213
BspHI TCATGA 3 cut(s) 738, 1450, 2866
BspLI GGNNCC 2 cut(s) 153, 1664
BspPI GGATC 6 cut(s) 596, 674, 1523, 1637, 2096, 2939
BspT107I GGYRCC 1 cut(s) 1662
BsrDI GCAATG 1 cut(s) 2300
BsrI ACTGG 6 cut(s) 849, 1030, 1310, 2271, 2452, 2726
BssECI CCNNGG 2 cut(s) 550, 1875
BssNAI GTATAC 1 cut(s) 834
BssT1I CCWWGG 1 cut(s) 1875
Bst1107I GTATAC 1 cut(s) 834
Bst2UI CCWGG 5 cut(s) 534, 654, 918, 2076, 2340
Bst6I CTCTTC 3 cut(s) 407, 1362, 2778
BstAPI GCANNNNNTGC 1 cut(s) 331
BstC8I GCNNGC 7 cut(s) 243, 557, 699, 1296, 1773, 2121, 2712
BstDSI CCRYGG 1 cut(s) 550
BstFNI CGCG 4 cut(s) 678, 791, 2100, 2213
BstMAI GTCTC 2 cut(s) 383, 1767
BstMWI GCNNNNNNNGC 2 cut(s) 131, 331
BstNI CCWGG 5 cut(s) 534, 654, 918, 2076, 2340
BstNSI RCATGY 5 cut(s) 350, 926, 1485, 2348, 2901
BstSCI CCNGG 7 cut(s) 532, 652, 722, 916, 2074, 2144, 2338
BstUI CGCG 4 cut(s) 678, 791, 2100, 2213
BstV1I GCAGC 2 cut(s) 768, 1709
BstV2I GAAGAC 5 cut(s) 299, 1125, 1269, 2541, 2685
BstX2I RGATCY 3 cut(s) 1398, 1629, 2814
BstXI CCANNNNNNTGG 1 cut(s) 1625
BstYI RGATCY 3 cut(s) 1398, 1629, 2814
BstZ17I GTATAC 1 cut(s) 834
Bsu15I ATCGAT 2 cut(s) 1498, 2914
Bsu36I CCTNAGG 1 cut(s) 1667
BsuRI GGCC 5 cut(s) 134, 549, 1191, 1943, 2607
BsuTUI ATCGAT 2 cut(s) 1498, 2914
BtgI CCRYGG 1 cut(s) 550
BtsIMutI CAGTG 2 cut(s) 843, 2265
Cac8I GCNNGC 7 cut(s) 243, 557, 699, 1296, 1773, 2121, 2712
CciI TCATGA 3 cut(s) 738, 1450, 2866
Cfr13I GGNCC 4 cut(s) 132, 152, 358, 1941
ClaI ATCGAT 2 cut(s) 1498, 2914
Csp6I GTAC 9 cut(s) 253, 674, 1473, 1596, 1842, 1902, 2096, 2158, 2889
CspCI CAANNNNNGTGG 2 cut(s) 2265, 2300
CviQI GTAC 9 cut(s) 253, 674, 1473, 1596, 1842, 1902, 2096, 2158, 2889
Eam1104I CTCTTC 3 cut(s) 407, 1362, 2778
EarI CTCTTC 3 cut(s) 407, 1362, 2778
Eco130I CCWWGG 1 cut(s) 1875
Eco47I GGWCC 2 cut(s) 152, 358
Eco57I CTGAAG 4 cut(s) 24, 1137, 1673, 2553
Eco81I CCTNAGG 1 cut(s) 1667
EcoO109I RGGNCCY 3 cut(s) 152, 358, 1941
EcoRI GAATTC 2 cut(s) 1094, 2510
EcoRII CCWGG 5 cut(s) 532, 652, 916, 2074, 2338
EcoT14I CCWWGG 1 cut(s) 1875
EcoT22I ATGCAT 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 1875
FauNDI CATATG 1 cut(s) 484
FbaI TGATCA 1 cut(s) 1846
FblI GTMKAC 7 cut(s) 216, 833, 1041, 1462, 1555, 2878, 2971
Fnu4HI GCNGC 2 cut(s) 757, 1698
Fsp4HI GCNGC 2 cut(s) 757, 1698
FspBI CTAG 4 cut(s) 1409, 1512, 2825, 2928
GluI GCNGC 2 cut(s) 757, 1698
HaeIII GGCC 5 cut(s) 134, 549, 1191, 1943, 2607
HapII CCGG 2 cut(s) 723, 2145
HincII GTYRAC 2 cut(s) 36, 1042
HindII GTYRAC 2 cut(s) 36, 1042
HindIII AAGCTT 4 cut(s) 1292, 1377, 1955, 2708
HpaII CCGG 2 cut(s) 723, 2145
HphI GGTGA 6 cut(s) 25, 838, 988, 1730, 2410, 3010
HpyAV CCTTC 3 cut(s) 424, 1264, 2680
HpyCH4IV ACGT 2 cut(s) 968, 1796
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 331
HpySE526I ACGT 2 cut(s) 968, 1796
Ksp22I TGATCA 1 cut(s) 1846
LmnI GCTCC 4 cut(s) 4, 238, 1384, 2800
Lsp1109I GCAGC 2 cut(s) 768, 1709
LweI GCATC 6 cut(s) 71, 1043, 1132, 1238, 2548, 2654
MaeI CTAG 4 cut(s) 1409, 1512, 2825, 2928
MaeII ACGT 2 cut(s) 968, 1796
MaeIII GTNAC 4 cut(s) 371, 883, 1906, 2305
MfeI CAATTG 2 cut(s) 879, 2301
MflI RGATCY 3 cut(s) 1398, 1629, 2814
MlyI GAGTC 6 cut(s) 628, 1468, 1663, 1769, 2050, 2884
MmeI TCCRAC 7 cut(s) 188, 641, 937, 1119, 2359, 2535, 3001
Mph1103I ATGCAT 1 cut(s) 64
MslI CAYNNNNRTG 2 cut(s) 971, 2393
MspA1I CMGCKG 1 cut(s) 1700
MspI CCGG 2 cut(s) 723, 2145
MspR9I CCNGG 7 cut(s) 534, 654, 724, 918, 2076, 2146, 2340
MunI CAATTG 2 cut(s) 879, 2301
Mva1269I GAATGC 2 cut(s) 1315, 2731
MvaI CCWGG 5 cut(s) 534, 654, 918, 2076, 2340
MvnI CGCG 4 cut(s) 678, 791, 2100, 2213
MwoI GCNNNNNNNGC 2 cut(s) 131, 331
NciI CCSGG 2 cut(s) 724, 2146
NdeI CATATG 1 cut(s) 484
NlaIV GGNNCC 2 cut(s) 153, 1664
NmuCI GTSAC 1 cut(s) 371
NsiI ATGCAT 1 cut(s) 64
NspI RCATGY 5 cut(s) 350, 926, 1485, 2348, 2901
PagI TCATGA 3 cut(s) 738, 1450, 2866
PciI ACATGT 2 cut(s) 922, 2344
PctI GAATGC 2 cut(s) 1315, 2731
PfeI GAWTC 5 cut(s) 713, 1051, 1447, 2010, 2863
PflMI CCANNNNNTGG 2 cut(s) 569, 653
PfoI TCCNGGA 4 cut(s) 652, 722, 2074, 2144
PkrI GCNGC 2 cut(s) 758, 1699
PleI GAGTC 6 cut(s) 628, 1467, 1662, 1768, 2050, 2883
PpsI GAGTC 6 cut(s) 628, 1467, 1662, 1768, 2050, 2883
PpuMI RGGWCCY 2 cut(s) 152, 358
PscI ACATGT 2 cut(s) 922, 2344
PshBI ATTAAT 2 cut(s) 807, 2393
Psp1406I AACGTT 1 cut(s) 1796
Psp5II RGGWCCY 2 cut(s) 152, 358
Psp6I CCWGG 5 cut(s) 532, 652, 916, 2074, 2338
PspGI CCWGG 5 cut(s) 532, 652, 916, 2074, 2338
PspN4I GGNNCC 2 cut(s) 153, 1664
PspPI GGNCC 4 cut(s) 132, 152, 358, 1941
PspPPI RGGWCCY 2 cut(s) 152, 358
PsuI RGATCY 3 cut(s) 1398, 1629, 2814
PvuII CAGCTG 1 cut(s) 1700
RsaI GTAC 9 cut(s) 254, 675, 1474, 1597, 1843, 1903, 2097, 2159, 2890
RsaNI GTAC 9 cut(s) 253, 674, 1473, 1596, 1842, 1902, 2096, 2158, 2889
RseI CAYNNNNRTG 2 cut(s) 971, 2393
SalI GTCGAC 1 cut(s) 1040
SatI GCNGC 2 cut(s) 757, 1698
Sau96I GGNCC 4 cut(s) 132, 152, 358, 1941
ScaI AGTACT 1 cut(s) 1903
SchI GAGTC 6 cut(s) 628, 1468, 1663, 1769, 2050, 2884
ScrFI CCNGG 7 cut(s) 534, 654, 724, 918, 2076, 2146, 2340
SfaNI GCATC 6 cut(s) 71, 1043, 1132, 1238, 2548, 2654
SinI GGWCC 2 cut(s) 152, 358
SmiMI CAYNNNNRTG 2 cut(s) 971, 2393
SsiI CCGC 6 cut(s) 66, 455, 789, 1216, 2211, 2632
SspI AATATT 3 cut(s) 752, 2135, 2174
SspMI CTAG 4 cut(s) 1409, 1512, 2825, 2928
StyD4I CCNGG 7 cut(s) 532, 652, 722, 916, 2074, 2144, 2338
StyI CCWWGG 1 cut(s) 1875
TaiI ACGT 2 cut(s) 971, 1799
TaqI TCGA 5 cut(s) 1041, 1225, 1498, 2641, 2914
TaqII GACCGA 1 cut(s) 269
TatI WGTACW 3 cut(s) 1841, 1901, 2157
TfiI GAWTC 5 cut(s) 713, 1051, 1447, 2010, 2863
TscAI CASTG 2 cut(s) 843, 2265
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 2 cut(s) 756, 1697
Tsp45I GTSAC 1 cut(s) 371
TspGWI ACGGA 1 cut(s) 601
TspRI CASTG 2 cut(s) 843, 2265
Van91I CCANNNNNTGG 2 cut(s) 569, 653
VpaK11BI GGWCC 2 cut(s) 152, 358
VspI ATTAAT 2 cut(s) 807, 2393
XapI RAATTY 5 cut(s) 307, 779, 1094, 2201, 2510
XbaI TCTAGA 2 cut(s) 1511, 2927
XceI RCATGY 5 cut(s) 350, 926, 1485, 2348, 2901
XmiI GTMKAC 7 cut(s) 216, 833, 1041, 1462, 1555, 2878, 2971
XspI CTAG 4 cut(s) 1409, 1512, 2825, 2928
ZrmI AGTACT 1 cut(s) 1903
Zsp2I ATGCAT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.