Rroxscaffold_5G00362880

tRNA rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006852
Physical Location & Seq
Forward (+)
43935911 .. 43941138
5228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00362880.1

Sequence Viewer

Length: 1167 bp
ATGAAGGGGAGATCTGTACATTTGAAGTTGAAGCGAGTAAAGGAGCTACTGCTACATAGGCAGTATGTTGGTGAAAGAGAATCCCTTGTATCATTTGTAAGCATAGGGGGTACTGTGAGCTTTGTCGCCGCCGACGCGGTTATAGGCCATGAGGGAGCCGACGACAATGCCAAGGACGAGAAGGGCAATGGCGAGAGCGATCTCTTTGATTGGAGGCTTGTTGTTGACGGTGTAGGAGGTCTCCATCATTATGTCTTCGTCGGTAATGGATCGAGAATGCGTGATCTACATACGCCATGGCCGAGATCTGCAGAATTGGGGGTGAGGATTTTAGGGTTTCGTAATTGGGAATTTGGGGTTTCGCAGTTAGCCGAGTTGAGCAAGGGGACTTTAGAGGGAGATGTTTCAGAGAAACTTGATGTTTATGCCCGTGCAAAAAAGGCGGTTCCTATCACTTGTAATCATCTGCGCAGTATCTCAAAGATTGATAGTGAACAAACCCCTCGGTTAATGCTGAAGATTTACGAGCTTCTGGTAATGCATGAAAATTTATCTGGACATTTTGACAGAGCTAAAAGCATAATTGGACGGCATAGAAGAAGAAGAGTCCCCAGCGACAGATCTTATGGAAAACGAAAGAGAGACTCGGCCCGGGAAGAGTTAGAGCGGAAGATAGCTCAAATGACTAATCGGATTGCAAGTATCAAAAGGGTTATTATCAGTCAGAATAAGGACATTGAAATAGTGAATAAATTGCCAACAGCCACATTGATTGATCCTAGAATCACTGTTGGATGGTGCAGGAGACATGGAGTTCCCATTGACAGTGTATTTCCCAAGTCCCTCTTGAGAAAGTTTGCTTGGGCAATGGATGTCGGCCCTGAGTTCAGATTCTTATCTTTGGAGATGCTCTGCCTTTCTGCTAAGGAGTGTGAGATATGTGATTTTTTTGTGTATATCCCACGGTATGGGGACGGCACTGCTTCCTTGAATGTTACTGTCGCAGCTTCTATTGTCCTGCATCAGTTTGGAGTCTGGGCTGGCTTCCCTGAGAGAACCCGTGATGGGAACAAGTTCATTGTGGCAGAGAAACCTGCAAAGCAGACAAGAAGAAGTTTCTGTGCAGAAACAGCAGATTCTGTAATTGATCGAAGAGCGAAAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

388

Amino Acids

43.87

Weight (kDa)

9.64

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topo_C_assoc PF14370 256 - 298 7.3e-16 C-terminal topoisomerase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 470
Acc36I ACCTGC 1 cut(s) 1102
AccB7I CCANNNNNTGG 1 cut(s) 968
AccBSI CCGCTC 1 cut(s) 667
AccII CGCG 1 cut(s) 137
AciI CCGC 4 cut(s) 129, 137, 443, 667
AclWI GGATC 2 cut(s) 277, 770
AcoI YGGCCR 1 cut(s) 299
AcsI RAATTY 2 cut(s) 350, 547
AcuI CTGAAG 1 cut(s) 536
AfaI GTAC 2 cut(s) 18, 112
AfiI CCNNNNNNNGG 2 cut(s) 968, 1065
AgsI TTSAA 4 cut(s) 25, 31, 740, 991
AjuI GAANNNNNNNTTGG 2 cut(s) 844, 876
AluBI AGCT 6 cut(s) 46, 120, 529, 572, 677, 1007
AluI AGCT 6 cut(s) 46, 120, 529, 572, 677, 1007
Alw26I GTCTC 3 cut(s) 245, 636, 799
AlwI GGATC 2 cut(s) 277, 770
AlwNI CAGNNNCTG 1 cut(s) 1139
Ama87I CYCGRG 1 cut(s) 651
AoxI GGCC 4 cut(s) 145, 299, 648, 877
ApeKI GCWGC 1 cut(s) 1004
ApoI RAATTY 2 cut(s) 350, 547
Asp700I GAANNNNTTC 1 cut(s) 1073
AspLEI GCGC 1 cut(s) 471
AspS9I GGNCC 2 cut(s) 649, 878
AsuC2I CCSGG 2 cut(s) 652, 653
AsuHPI GGTGA 2 cut(s) 83, 334
AvaI CYCGRG 1 cut(s) 651
BbsI GAAGAC 1 cut(s) 247
BbvI GCAGC 1 cut(s) 1016
BccI CCATC 3 cut(s) 252, 789, 1058
BceAI ACGGC 2 cut(s) 605, 991
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BcnI CCSGG 2 cut(s) 652, 653
BcoDI GTCTC 3 cut(s) 245, 636, 799
BfaI CTAG 2 cut(s) 780, 1165
BfmI CTRYAG 1 cut(s) 309
BfuAI ACCTGC 1 cut(s) 1102
BglII AGATCT 3 cut(s) 11, 305, 620
BisI GCNGC 2 cut(s) 129, 1005
BlsI GCNGC 2 cut(s) 130, 1006
Bme1390I CCNGG 2 cut(s) 652, 653
BmeT110I CYCGRG 1 cut(s) 651
BmgT120I GGNCC 2 cut(s) 649, 878
BmiI GGNNCC 2 cut(s) 157, 447
BmrFI CCNGG 2 cut(s) 652, 653
BmsI GCATC 2 cut(s) 897, 1030
BpiI GAAGAC 1 cut(s) 247
Bpu10I CCTNAGC 1 cut(s) 924
BpuEI CTTGAG 1 cut(s) 868
BpuMI CCSGG 2 cut(s) 652, 653
BsaBI GATNNNNATC 1 cut(s) 895
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 5 cut(s) 171, 296, 503, 651, 962
Bsc4I CCNNNNNNNGG 2 cut(s) 968, 1065
Bse3DI GCAATG 2 cut(s) 193, 873
Bse8I GATNNNNATC 1 cut(s) 895
BseDI CCNNGG 5 cut(s) 171, 296, 503, 651, 962
BseGI GGATG 2 cut(s) 800, 877
BseJI GATNNNNATC 1 cut(s) 895
BseLI CCNNNNNNNGG 2 cut(s) 968, 1065
BseMI GCAATG 2 cut(s) 193, 873
BseMII CTCAG 2 cut(s) 873, 1041
BseXI GCAGC 1 cut(s) 1016
BseYI CCCAGC 1 cut(s) 611
BsgI GTGCAG 2 cut(s) 820, 1143
Bsh1236I CGCG 1 cut(s) 137
BshFI GGCC 4 cut(s) 147, 301, 650, 879
BsiHKCI CYCGRG 1 cut(s) 651
BsiSI CCGG 1 cut(s) 652
BslFI GGGAC 4 cut(s) 400, 593, 826, 986
BslI CCNNNNNNNGG 2 cut(s) 968, 1065
BsmAI GTCTC 3 cut(s) 245, 636, 799
BsmFI GGGAC 4 cut(s) 400, 593, 826, 986
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 4 cut(s) 147, 301, 650, 879
Bso31I GGTCTC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 651
Bsp1407I TGTACA 1 cut(s) 16
Bsp143I GATC 8 cut(s) 11, 199, 269, 283, 305, 620, 775, 1147
Bsp19I CCATGG 1 cut(s) 296
BspACI CCGC 4 cut(s) 129, 137, 443, 667
BspANI GGCC 4 cut(s) 147, 301, 650, 879
BspCNI CTCAG 2 cut(s) 874, 1042
BspFNI CGCG 1 cut(s) 137
BspLI GGNNCC 2 cut(s) 157, 447
BspMAI CTGCAG 1 cut(s) 313
BspMI ACCTGC 1 cut(s) 1102
BspPI GGATC 2 cut(s) 277, 770
BspQI GCTCTTC 1 cut(s) 1147
BspTNI GGTCTC 1 cut(s) 245
BsrBI CCGCTC 1 cut(s) 667
BsrDI GCAATG 2 cut(s) 193, 873
BsrGI TGTACA 1 cut(s) 16
BssECI CCNNGG 5 cut(s) 171, 296, 503, 651, 962
BssMI GATC 8 cut(s) 11, 199, 269, 283, 305, 620, 775, 1147
BssT1I CCWWGG 2 cut(s) 171, 296
Bst4CI ACNGT 6 cut(s) 115, 230, 790, 827, 966, 1000
Bst6I CTCTTC 3 cut(s) 598, 651, 1147
BstAUI TGTACA 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 1042
BstDEI CTNAG 3 cut(s) 882, 924, 1050
BstDSI CCRYGG 2 cut(s) 296, 962
BstF5I GGATG 2 cut(s) 800, 877
BstFNI CGCG 1 cut(s) 137
BstHHI GCGC 1 cut(s) 471
BstKTI GATC 8 cut(s) 14, 202, 272, 286, 308, 623, 778, 1150
BstMAI GTCTC 3 cut(s) 245, 636, 799
BstMBI GATC 8 cut(s) 11, 199, 269, 283, 305, 620, 775, 1147
BstMWI GCNNNNNNNGC 4 cut(s) 58, 134, 440, 1130
BstSCI CCNGG 2 cut(s) 650, 651
BstSFI CTRYAG 1 cut(s) 309
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 1 cut(s) 1016
BstV2I GAAGAC 1 cut(s) 247
BstX2I RGATCY 3 cut(s) 11, 305, 620
BstYI RGATCY 3 cut(s) 11, 305, 620
BsuRI GGCC 4 cut(s) 147, 301, 650, 879
BtgI CCRYGG 2 cut(s) 296, 962
BtsCI GGATG 2 cut(s) 800, 877
BtsI GCAGTG 1 cut(s) 978
BtsIMutI CAGTG 3 cut(s) 786, 832, 978
BveI ACCTGC 1 cut(s) 1102
Cac8I GCNNGC 1 cut(s) 1042
CaiI CAGNNNCTG 1 cut(s) 1139
CfoI GCGC 1 cut(s) 471
Cfr13I GGNCC 2 cut(s) 649, 878
Cfr9I CCCGGG 1 cut(s) 651
CseI GACGC 1 cut(s) 143
Csp6I GTAC 2 cut(s) 17, 111
CspCI CAANNNNNGTGG 2 cut(s) 754, 789
CviAII CATG 4 cut(s) 149, 297, 542, 809
CviQI GTAC 2 cut(s) 17, 111
DdeI CTNAG 3 cut(s) 882, 924, 1050
DpnI GATC 8 cut(s) 13, 201, 271, 285, 307, 622, 777, 1149
DpnII GATC 8 cut(s) 11, 199, 269, 283, 305, 620, 775, 1147
EaeI YGGCCR 1 cut(s) 299
Eam1104I CTCTTC 3 cut(s) 598, 651, 1147
EarI CTCTTC 3 cut(s) 598, 651, 1147
Eco130I CCWWGG 2 cut(s) 171, 296
Eco31I GGTCTC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 536
Eco88I CYCGRG 1 cut(s) 651
EcoT14I CCWWGG 2 cut(s) 171, 296
EcoT22I ATGCAT 1 cut(s) 543
ErhI CCWWGG 2 cut(s) 171, 296
FaeI CATG 4 cut(s) 152, 300, 545, 812
FalI AAGNNNNNCTT 2 cut(s) 830, 862
FaqI GGGAC 4 cut(s) 400, 593, 826, 986
FatI CATG 4 cut(s) 148, 296, 541, 808
Fnu4HI GCNGC 2 cut(s) 129, 1005
FokI GGATG 2 cut(s) 807, 884
Fsp4HI GCNGC 2 cut(s) 129, 1005
FspBI CTAG 2 cut(s) 780, 1165
FspI TGCGCA 1 cut(s) 470
GlaI GCGC 1 cut(s) 470
GluI GCNGC 2 cut(s) 129, 1005
GsaI CCCAGC 1 cut(s) 615
HaeIII GGCC 4 cut(s) 147, 301, 650, 879
HapII CCGG 1 cut(s) 652
HgaI GACGC 1 cut(s) 143
HhaI GCGC 1 cut(s) 471
Hin1II CATG 4 cut(s) 152, 300, 545, 812
Hin6I GCGC 1 cut(s) 469
HinP1I GCGC 1 cut(s) 469
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 7 cut(s) 80, 606, 644, 783, 891, 1032, 1136
HpaII CCGG 1 cut(s) 652
HphI GGTGA 2 cut(s) 83, 334
Hpy166II GTNNAC 2 cut(s) 226, 494
Hpy188I TCNGA 4 cut(s) 409, 693, 726, 890
Hpy188III TCNNGA 3 cut(s) 273, 555, 847
Hpy8I GTNNAC 2 cut(s) 226, 494
Hpy99I CGWCG 3 cut(s) 137, 164, 263
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 6 cut(s) 115, 230, 790, 827, 966, 1000
HpyCH4V TGCA 8 cut(s) 311, 434, 541, 698, 801, 1021, 1097, 1124
HpyF10VI GCNNNNNNNGC 4 cut(s) 58, 134, 440, 1130
HpyF3I CTNAG 3 cut(s) 882, 924, 1050
Hsp92II CATG 4 cut(s) 152, 300, 545, 812
HspAI GCGC 1 cut(s) 469
Kzo9I GATC 8 cut(s) 11, 199, 269, 283, 305, 620, 775, 1147
LguI GCTCTTC 1 cut(s) 1147
LmnI GCTCC 2 cut(s) 43, 155
Lsp1109I GCAGC 1 cut(s) 1016
LweI GCATC 2 cut(s) 897, 1030
MaeI CTAG 2 cut(s) 780, 1165
MaeIII GTNAC 1 cut(s) 994
MalI GATC 8 cut(s) 13, 201, 271, 285, 307, 622, 777, 1149
MbiI CCGCTC 1 cut(s) 667
MboI GATC 8 cut(s) 11, 199, 269, 283, 305, 620, 775, 1147
MboII GAAGA 9 cut(s) 247, 529, 609, 612, 615, 668, 682, 1122, 1164
MflI RGATCY 3 cut(s) 11, 305, 620
MluCI AATT 7 cut(s) 314, 343, 350, 547, 582, 752, 1143
MlyI GAGTC 3 cut(s) 615, 638, 1041
MmeI TCCRAC 1 cut(s) 772
MnlI CCTC 7 cut(s) 145, 207, 230, 318, 388, 513, 854
Mph1103I ATGCAT 1 cut(s) 543
MroXI GAANNNNTTC 1 cut(s) 1073
MseI TTAA 1 cut(s) 509
MslI CAYNNNNRTG 1 cut(s) 249
MspI CCGG 1 cut(s) 652
MspR9I CCNGG 2 cut(s) 652, 653
Mva1269I GAATGC 1 cut(s) 282
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 4 cut(s) 58, 134, 440, 1130
NciI CCSGG 2 cut(s) 652, 653
NcoI CCATGG 1 cut(s) 296
NdeII GATC 8 cut(s) 11, 199, 269, 283, 305, 620, 775, 1147
NlaIII CATG 4 cut(s) 152, 300, 545, 812
NlaIV GGNNCC 2 cut(s) 157, 447
NmeAIII GCCGAG 3 cut(s) 327, 397, 626
NsbI TGCGCA 1 cut(s) 470
NsiI ATGCAT 1 cut(s) 543
PciSI GCTCTTC 1 cut(s) 1147
PcsI WCGNNNNNNNCGW 1 cut(s) 299
PctI GAATGC 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 1073
PfeI GAWTC 4 cut(s) 80, 783, 891, 1136
PflMI CCANNNNNTGG 1 cut(s) 968
PkrI GCNGC 2 cut(s) 130, 1006
PleI GAGTC 3 cut(s) 614, 638, 1040
PpsI GAGTC 3 cut(s) 614, 638, 1040
PspFI CCCAGC 1 cut(s) 611
PspN4I GGNNCC 2 cut(s) 157, 447
PspPI GGNCC 2 cut(s) 649, 878
PstI CTGCAG 1 cut(s) 313
PstNI CAGNNNCTG 1 cut(s) 1139
PsuI RGATCY 3 cut(s) 11, 305, 620
RsaI GTAC 2 cut(s) 18, 112
RsaNI GTAC 2 cut(s) 17, 111
RseI CAYNNNNRTG 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 1147
SaqAI TTAA 1 cut(s) 509
SatI GCNGC 2 cut(s) 129, 1005
Sau3AI GATC 8 cut(s) 11, 199, 269, 283, 305, 620, 775, 1147
Sau96I GGNCC 2 cut(s) 649, 878
SchI GAGTC 3 cut(s) 615, 638, 1041
ScrFI CCNGG 2 cut(s) 652, 653
SetI ASST 8 cut(s) 48, 122, 241, 531, 574, 679, 1009, 1096
SfaNI GCATC 2 cut(s) 897, 1030
SfcI CTRYAG 1 cut(s) 309
SmaI CCCGGG 1 cut(s) 653
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 847
SmoI CTYRAG 1 cut(s) 847
Sse9I AATT 7 cut(s) 314, 343, 350, 547, 582, 752, 1143
SsiI CCGC 4 cut(s) 129, 137, 443, 667
SspMI CTAG 2 cut(s) 780, 1165
StyD4I CCNGG 2 cut(s) 650, 651
StyI CCWWGG 2 cut(s) 171, 296
TaaI ACNGT 6 cut(s) 115, 230, 790, 827, 966, 1000
TaqI TCGA 2 cut(s) 272, 1150
TasI AATT 7 cut(s) 314, 343, 350, 547, 582, 752, 1143
TatI WGTACW 1 cut(s) 16
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 4 cut(s) 80, 783, 891, 1136
Tru1I TTAA 1 cut(s) 509
Tru9I TTAA 1 cut(s) 509
TscAI CASTG 3 cut(s) 793, 832, 985
TseI GCWGC 1 cut(s) 1004
TspDTI ATGAA 3 cut(s) 17, 558, 1066
TspMI CCCGGG 1 cut(s) 651
TspRI CASTG 3 cut(s) 793, 832, 985
Van91I CCANNNNNTGG 1 cut(s) 968
XapI RAATTY 2 cut(s) 350, 547
XmaI CCCGGG 1 cut(s) 651
XmnI GAANNNNTTC 1 cut(s) 1073
XspI CTAG 2 cut(s) 780, 1165
Zsp2I ATGCAT 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.