RchiOBHm_Chr4g0421651

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006852
Physical Location & Seq
Reverse (-)
47300022 .. 47300234
213 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39128

Sequence Viewer

Length: 213 bp
ATGAAGAAGATTCATGAGCTCAGTCTACAAGAGCATACCATAGCCTGTAGTCAAACTTATCGATTAGAAACATCCAGAGATAATTACCTTGATCCCAGGATTACTGTTGCATGGTGTCTACGACATCGGATTTCAGTTTCTAAGATTTTTGACTCGCGTCTCCGAAACAAATTCATGTGGACAATGAATGTGGAGTCTGATTTTAGATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

70

Amino Acids

8.57

Weight (kDa)

9.44

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topo_C_assoc PF14370 2 - 69 2.2e-21 C-terminal topoisomerase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 25, 118
AccII CGCG 1 cut(s) 157
AclWI GGATC 1 cut(s) 86
AcsI RAATTY 1 cut(s) 170
AjnI CCWGG 1 cut(s) 95
AluBI AGCT 1 cut(s) 19
AluI AGCT 1 cut(s) 19
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 170
BanII GRGCYC 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 21
BciT130I CCWGG 1 cut(s) 97
BcoDI GTCTC 1 cut(s) 164
BfmI CTRYAG 1 cut(s) 46
Bme1390I CCNGG 1 cut(s) 97
BmrFI CCNGG 1 cut(s) 97
BoxI GACNNNNGTC 1 cut(s) 156
Bsa29I ATCGAT 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 95
BseBI CCWGG 1 cut(s) 97
BseCI ATCGAT 1 cut(s) 61
BseDI CCNNGG 1 cut(s) 95
BseGI GGATG 1 cut(s) 71
BseMII CTCAG 1 cut(s) 34
Bsh1236I CGCG 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 21
BsmAI GTCTC 1 cut(s) 164
BsmBI CGTCTC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 1 cut(s) 91
BspCNI CTCAG 1 cut(s) 33
BspDI ATCGAT 1 cut(s) 61
BspFNI CGCG 1 cut(s) 157
BspHI TCATGA 1 cut(s) 13
BspPI GGATC 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 1 cut(s) 91
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 1 cut(s) 106
BstDEI CTNAG 2 cut(s) 20, 141
BstF5I GGATG 1 cut(s) 71
BstFNI CGCG 1 cut(s) 157
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 1 cut(s) 91
BstNI CCWGG 1 cut(s) 97
BstPAI GACNNNNGTC 1 cut(s) 156
BstSCI CCNGG 1 cut(s) 95
BstSFI CTRYAG 1 cut(s) 46
BstUI CGCG 1 cut(s) 157
Bsu15I ATCGAT 1 cut(s) 61
BsuTUI ATCGAT 1 cut(s) 61
BtsCI GGATG 1 cut(s) 71
CciI TCATGA 1 cut(s) 13
ClaI ATCGAT 1 cut(s) 61
CseI GACGC 1 cut(s) 146
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CviAII CATG 3 cut(s) 14, 111, 175
CviJI RGCY 2 cut(s) 19, 44
CviKI_1 RGCY 2 cut(s) 19, 44
DdeI CTNAG 2 cut(s) 20, 141
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 21
Eco53kI GAGCTC 1 cut(s) 19
EcoICRI GAGCTC 1 cut(s) 19
EcoRII CCWGG 1 cut(s) 95
EcoT38I GRGCYC 1 cut(s) 21
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 3 cut(s) 17, 114, 178
FaiI YATR 5 cut(s) 15, 36, 41, 112, 176
FatI CATG 3 cut(s) 13, 110, 174
FblI GTMKAC 2 cut(s) 25, 118
FokI GGATG 1 cut(s) 58
FriOI GRGCYC 1 cut(s) 21
HgaI GACGC 1 cut(s) 146
Hin1II CATG 3 cut(s) 17, 114, 178
HinfI GANTC 3 cut(s) 10, 152, 194
Hpy166II GTNNAC 3 cut(s) 26, 119, 180
Hpy188I TCNGA 3 cut(s) 129, 164, 199
Hpy188III TCNNGA 2 cut(s) 14, 75
Hpy8I GTNNAC 3 cut(s) 26, 119, 180
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4V TGCA 1 cut(s) 110
HpyF3I CTNAG 2 cut(s) 20, 141
Hsp92II CATG 3 cut(s) 17, 114, 178
Kzo9I GATC 1 cut(s) 91
LpnPI CCDG 4 cut(s) 58, 82, 88, 109
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 2 cut(s) 16, 19
MhlI GDGCHC 1 cut(s) 21
MluCI AATT 2 cut(s) 82, 170
MlyI GAGTC 2 cut(s) 146, 203
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MvnI CGCG 1 cut(s) 157
NdeII GATC 1 cut(s) 91
NlaIII CATG 3 cut(s) 17, 114, 178
PagI TCATGA 1 cut(s) 13
PfeI GAWTC 1 cut(s) 10
PleI GAGTC 2 cut(s) 146, 202
PpsI GAGTC 2 cut(s) 146, 202
PshAI GACNNNNGTC 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 21
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
SacI GAGCTC 1 cut(s) 21
Sau3AI GATC 1 cut(s) 91
SchI GAGTC 2 cut(s) 146, 203
ScrFI CCNGG 1 cut(s) 97
SduI GDGCHC 1 cut(s) 21
SetI ASST 2 cut(s) 21, 90
SfcI CTRYAG 1 cut(s) 46
Sse9I AATT 2 cut(s) 82, 170
SstI GAGCTC 1 cut(s) 21
StyD4I CCNGG 1 cut(s) 95
TaaI ACNGT 1 cut(s) 106
TaqI TCGA 1 cut(s) 61
TasI AATT 2 cut(s) 82, 170
TfiI GAWTC 1 cut(s) 10
TspDTI ATGAA 3 cut(s) 17, 163, 200
XapI RAATTY 1 cut(s) 170
XmiI GTMKAC 2 cut(s) 25, 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.