Rh4CG248600

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006852
Physical Location & Seq
Reverse (-)
49815424 .. 49816077
654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG248600.1

Sequence Viewer

Length: 654 bp
ATGGAGGTGTCATGCTATCGAATTGAACCACCTGGATTATTTATAGGTCGTGGCAAGCACCCAAAGAATGGAATGTTAAAAAGACGAATCAACCCAAGTGATGTTGTCATCAACGTTGGAGATACAAGTCAGACTCCACGCTGCCTCATTCCTGGATGTTGGAAGAAAGTTATACGCGATCCTCATCTTAAATGGCTCCCATATTGGAACGATTCAATTTCCGAGAGAAGAAAGTACTTGAACTTGAAATATTACAGCAGTATGTTTGACATTTCAAAATTTAACACCCCGAGGGAACTAAAGATCAATCAAACAGAGATCAAGCAACAATACACAATGGATTTCACCAGTTCAAATCTTGTGGATAAGCAATGTGCAATTGTTACATATCTTATTGATACATTAGCTTTAAGACCTGGACATGTTAAGGACAAAGAAATTACTGCACCTACTGTTGGATGTTGCACGTTAATGGTGAAAAATGTAATTTTACATGAACCCTCATGTTTGGAGTTCACTTTATTGGGAAAGCATCAGTCGACTACGAGAATGAGGTTGAAGTTGAAAACCTTGTGTTTAATTGCATTAAAGAATTTCATATTGGGAAATCTCCTGAAGACAATTTGTTTGACAAGTTGGATGACAAAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

217

Amino Acids

25.02

Weight (kDa)

9.59

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I_N PF02919 2 - 81 5.8e-27 Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I PF01028 89 - 197 6.5e-23 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 68
AccI GTMKAC 1 cut(s) 539
AccII CGCG 1 cut(s) 177
AclI AACGTT 1 cut(s) 114
AclWI GGATC 1 cut(s) 173
AcsI RAATTY 2 cut(s) 278, 592
AcuI CTGAAG 1 cut(s) 635
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 2 cut(s) 68, 455
AflIII ACRYGT 1 cut(s) 421
AgsI TTSAA 8 cut(s) 26, 216, 241, 247, 276, 354, 559, 565
AjnI CCWGG 3 cut(s) 31, 151, 415
AjuI GAANNNNNNNTTGG 2 cut(s) 584, 616
AluBI AGCT 1 cut(s) 407
AluI AGCT 1 cut(s) 407
AlwI GGATC 1 cut(s) 173
Ama87I CYCGRG 1 cut(s) 289
ApeKI GCWGC 1 cut(s) 141
ApoI RAATTY 2 cut(s) 278, 592
ArsI GACNNNNNNTTYG 2 cut(s) 610, 642
AsuHPI GGTGA 2 cut(s) 337, 487
AvaI CYCGRG 1 cut(s) 289
BbsI GAAGAC 1 cut(s) 623
BbvI GCAGC 1 cut(s) 128
BciT130I CCWGG 3 cut(s) 33, 153, 417
BisI GCNGC 1 cut(s) 142
BlsI GCNGC 1 cut(s) 143
BmcAI AGTACT 1 cut(s) 236
Bme1390I CCNGG 3 cut(s) 33, 153, 417
BmeT110I CYCGRG 1 cut(s) 289
BmiI GGNNCC 1 cut(s) 197
BmrFI CCNGG 3 cut(s) 33, 153, 417
BmsI GCATC 1 cut(s) 541
BpiI GAAGAC 1 cut(s) 623
BsaBI GATNNNNATC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 290
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 455
Bse1I ACTGG 1 cut(s) 348
Bse3DI GCAATG 1 cut(s) 377
Bse8I GATNNNNATC 1 cut(s) 183
BseBI CCWGG 3 cut(s) 33, 153, 417
BseDI CCNNGG 1 cut(s) 290
BseGI GGATG 3 cut(s) 161, 464, 645
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 2 cut(s) 68, 455
BseMI GCAATG 1 cut(s) 377
BseNI ACTGG 1 cut(s) 348
BseXI GCAGC 1 cut(s) 128
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 177
BsiHKCI CYCGRG 1 cut(s) 289
BslI CCNNNNNNNGG 2 cut(s) 68, 455
BsoBI CYCGRG 1 cut(s) 289
Bsp143I GATC 3 cut(s) 178, 303, 318
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 197
BspPI GGATC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 377
BsrI ACTGG 1 cut(s) 348
BssECI CCNNGG 1 cut(s) 290
BssMI GATC 3 cut(s) 178, 303, 318
Bst2UI CCWGG 3 cut(s) 33, 153, 417
Bst4CI ACNGT 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 56
BstF5I GGATG 3 cut(s) 161, 464, 645
BstFNI CGCG 1 cut(s) 177
BstKTI GATC 3 cut(s) 181, 306, 321
BstMBI GATC 3 cut(s) 178, 303, 318
BstNI CCWGG 3 cut(s) 33, 153, 417
BstNSI RCATGY 1 cut(s) 425
BstSCI CCNGG 3 cut(s) 31, 151, 415
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 1 cut(s) 128
BstV2I GAAGAC 1 cut(s) 623
BtsCI GGATG 3 cut(s) 161, 464, 645
Cac8I GCNNGC 1 cut(s) 56
Csp6I GTAC 1 cut(s) 235
CspCI CAANNNNNGTGG 2 cut(s) 342, 377
CviAII CATG 4 cut(s) 12, 422, 494, 504
CviJI RGCY 2 cut(s) 196, 407
CviKI_1 RGCY 2 cut(s) 196, 407
CviQI GTAC 1 cut(s) 235
DpnI GATC 3 cut(s) 180, 305, 320
DpnII GATC 3 cut(s) 178, 303, 318
Eco57I CTGAAG 1 cut(s) 635
Eco88I CYCGRG 1 cut(s) 289
EcoRII CCWGG 3 cut(s) 31, 151, 415
FaeI CATG 4 cut(s) 15, 425, 497, 507
FatI CATG 4 cut(s) 11, 421, 493, 503
FblI GTMKAC 1 cut(s) 539
Fnu4HI GCNGC 1 cut(s) 142
FokI GGATG 2 cut(s) 168, 471
Fsp4HI GCNGC 1 cut(s) 142
GluI GCNGC 1 cut(s) 142
Hin1II CATG 4 cut(s) 15, 425, 497, 507
HincII GTYRAC 1 cut(s) 540
HindII GTYRAC 1 cut(s) 540
HinfI GANTC 3 cut(s) 87, 133, 212
HphI GGTGA 2 cut(s) 337, 487
Hpy166II GTNNAC 2 cut(s) 516, 540
Hpy188I TCNGA 2 cut(s) 132, 223
Hpy188III TCNNGA 1 cut(s) 613
Hpy8I GTNNAC 2 cut(s) 516, 540
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4IV ACGT 2 cut(s) 114, 467
HpyCH4V TGCA 4 cut(s) 377, 446, 465, 584
HpySE526I ACGT 2 cut(s) 114, 467
Hsp92II CATG 4 cut(s) 15, 425, 497, 507
Kzo9I GATC 3 cut(s) 178, 303, 318
LmnI GCTCC 1 cut(s) 201
LpnPI CCDG 8 cut(s) 18, 45, 138, 165, 361, 402, 429, 626
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 1 cut(s) 541
MaeII ACGT 2 cut(s) 114, 467
MaeIII GTNAC 1 cut(s) 382
MalI GATC 3 cut(s) 180, 305, 320
MboI GATC 3 cut(s) 178, 303, 318
MboII GAAGA 3 cut(s) 175, 240, 628
MfeI CAATTG 1 cut(s) 378
MlyI GAGTC 1 cut(s) 127
MmeI TCCRAC 4 cut(s) 97, 140, 436, 617
MnlI CCTC 5 cut(s) 155, 192, 285, 511, 546
MseI TTAA 8 cut(s) 77, 189, 282, 410, 426, 470, 578, 587
MslI CAYNNNNRTG 1 cut(s) 470
MspR9I CCNGG 3 cut(s) 33, 153, 417
MunI CAATTG 1 cut(s) 378
MvaI CCWGG 3 cut(s) 33, 153, 417
MvnI CGCG 1 cut(s) 177
NdeII GATC 3 cut(s) 178, 303, 318
NlaIII CATG 4 cut(s) 15, 425, 497, 507
NlaIV GGNNCC 1 cut(s) 197
NspI RCATGY 1 cut(s) 425
PciI ACATGT 1 cut(s) 421
PfeI GAWTC 2 cut(s) 87, 212
PflMI CCANNNNNTGG 1 cut(s) 68
PfoI TCCNGGA 1 cut(s) 151
PkrI GCNGC 1 cut(s) 143
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PscI ACATGT 1 cut(s) 421
Psp1406I AACGTT 1 cut(s) 114
Psp6I CCWGG 3 cut(s) 31, 151, 415
PspGI CCWGG 3 cut(s) 31, 151, 415
PspN4I GGNNCC 1 cut(s) 197
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
RseI CAYNNNNRTG 1 cut(s) 470
SalI GTCGAC 1 cut(s) 538
SaqAI TTAA 8 cut(s) 77, 189, 282, 410, 426, 470, 578, 587
SatI GCNGC 1 cut(s) 142
Sau3AI GATC 3 cut(s) 178, 303, 318
ScaI AGTACT 1 cut(s) 236
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 3 cut(s) 33, 153, 417
SfaNI GCATC 1 cut(s) 541
SmiMI CAYNNNNRTG 1 cut(s) 470
SspI AATATT 1 cut(s) 251
StyD4I CCNGG 3 cut(s) 31, 151, 415
TaaI ACNGT 1 cut(s) 454
TaiI ACGT 2 cut(s) 117, 470
TaqI TCGA 2 cut(s) 19, 539
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 2 cut(s) 87, 212
Tru1I TTAA 8 cut(s) 77, 189, 282, 410, 426, 470, 578, 587
Tru9I TTAA 8 cut(s) 77, 189, 282, 410, 426, 470, 578, 587
TseI GCWGC 1 cut(s) 141
TspDTI ATGAA 2 cut(s) 510, 586
Van91I CCANNNNNTGG 1 cut(s) 68
XapI RAATTY 2 cut(s) 278, 592
XceI RCATGY 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 539
ZrmI AGTACT 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.