Rh4BG236800

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0006852
Physical Location & Seq
Reverse (-)
40260025 .. 40260396
372 bp
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UTR
Exon/CDS
Intron
Rh4BG236800.1

Sequence Viewer

Length: 372 bp
ATGGAGGTGTCATGCTATCGAATTGAAACACCTGAATTATTTATAGACCGAGGCAAGCACCCAAAGAATGGAATGTTAAAAAGACGAATCAACCCAAGTGATGTTGTCATTAACGTTGGAGATACAAGTCAGACTCCACGCTGCCCCATTCCTGGATGTTGGAAGAAAGTTGTATGTGATCCTCATCTTAAATGGCTCCCATATTGGAAAGATTCAATTTCTGGGAGAAGAAAGTACATGAACTTGAAATATTGCAGCAGTATGTTTAACATTTCAAAATTTAACACCGCGAGGGAACTAAAGATCAATCAAGTAGAGATCAAGCAACAATACACAGTGGATTTCACCAATTCAAATCTTGTGGATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

123

Amino Acids

14.43

Weight (kDa)

9.49

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I_N PF02919 2 - 81 5.3e-26 Eukaryotic DNA topoisomerase I, DNA binding fragment
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 68
AccII CGCG 1 cut(s) 290
AciI CCGC 1 cut(s) 288
AclI AACGTT 1 cut(s) 114
AclWI GGATC 1 cut(s) 173
AcsI RAATTY 1 cut(s) 278
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 2 cut(s) 68, 152
AgsI TTSAA 5 cut(s) 26, 216, 247, 276, 354
AjnI CCWGG 1 cut(s) 151
AlwI GGATC 1 cut(s) 173
ApeKI GCWGC 2 cut(s) 141, 255
ApoI RAATTY 1 cut(s) 278
AsuHPI GGTGA 1 cut(s) 337
BbvI GCAGC 2 cut(s) 128, 267
BciT130I CCWGG 1 cut(s) 153
BisI GCNGC 2 cut(s) 142, 256
BlsI GCNGC 2 cut(s) 143, 257
Bme1390I CCNGG 1 cut(s) 153
BmiI GGNNCC 1 cut(s) 197
BmrFI CCNGG 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 152
Bse8I GATNNNNATC 1 cut(s) 183
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 1 cut(s) 161
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 2 cut(s) 68, 152
BseXI GCAGC 2 cut(s) 128, 267
Bsh1236I CGCG 1 cut(s) 290
BslI CCNNNNNNNGG 2 cut(s) 68, 152
Bsp143I GATC 3 cut(s) 178, 303, 318
BspACI CCGC 1 cut(s) 288
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 1 cut(s) 197
BspPI GGATC 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 3 cut(s) 178, 303, 318
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 56
BstF5I GGATG 1 cut(s) 161
BstFNI CGCG 1 cut(s) 290
BstKTI GATC 3 cut(s) 181, 306, 321
BstMBI GATC 3 cut(s) 178, 303, 318
BstNI CCWGG 1 cut(s) 153
BstSCI CCNGG 1 cut(s) 151
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 2 cut(s) 128, 267
BtsCI GGATG 1 cut(s) 161
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 56
Csp6I GTAC 1 cut(s) 235
CspCI CAANNNNNGTGG 1 cut(s) 342
CviAII CATG 2 cut(s) 12, 238
CviJI RGCY 1 cut(s) 196
CviKI_1 RGCY 1 cut(s) 196
CviQI GTAC 1 cut(s) 235
DpnI GATC 3 cut(s) 180, 305, 320
DpnII GATC 3 cut(s) 178, 303, 318
EcoRII CCWGG 1 cut(s) 151
FaeI CATG 2 cut(s) 15, 241
FaiI YATR 6 cut(s) 13, 44, 175, 202, 239, 263
FatI CATG 2 cut(s) 11, 237
Fnu4HI GCNGC 2 cut(s) 142, 256
FokI GGATG 1 cut(s) 168
Fsp4HI GCNGC 2 cut(s) 142, 256
GluI GCNGC 2 cut(s) 142, 256
Hin1II CATG 2 cut(s) 15, 241
HinfI GANTC 3 cut(s) 87, 133, 212
HphI GGTGA 1 cut(s) 337
Hpy188I TCNGA 1 cut(s) 132
HpyCH4III ACNGT 1 cut(s) 337
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 1 cut(s) 255
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 2 cut(s) 15, 241
Kzo9I GATC 3 cut(s) 178, 303, 318
LmnI GCTCC 1 cut(s) 201
LpnPI CCDG 4 cut(s) 45, 138, 165, 207
Lsp1109I GCAGC 2 cut(s) 128, 267
MaeII ACGT 1 cut(s) 114
MalI GATC 3 cut(s) 180, 305, 320
MboI GATC 3 cut(s) 178, 303, 318
MboII GAAGA 2 cut(s) 175, 240
MluCI AATT 5 cut(s) 21, 35, 216, 278, 349
MlyI GAGTC 1 cut(s) 127
MmeI TCCRAC 2 cut(s) 97, 140
MnlI CCTC 3 cut(s) 44, 192, 285
MseI TTAA 5 cut(s) 77, 111, 189, 267, 282
MspR9I CCNGG 1 cut(s) 153
MvaI CCWGG 1 cut(s) 153
MvnI CGCG 1 cut(s) 290
NdeII GATC 3 cut(s) 178, 303, 318
NlaIII CATG 2 cut(s) 15, 241
NlaIV GGNNCC 1 cut(s) 197
PfeI GAWTC 2 cut(s) 87, 212
PflMI CCANNNNNTGG 1 cut(s) 68
PfoI TCCNGGA 1 cut(s) 151
PkrI GCNGC 2 cut(s) 143, 257
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
Psp1406I AACGTT 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 151
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 1 cut(s) 197
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
SaqAI TTAA 5 cut(s) 77, 111, 189, 267, 282
SatI GCNGC 2 cut(s) 142, 256
Sau3AI GATC 3 cut(s) 178, 303, 318
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 1 cut(s) 153
SetI ASST 3 cut(s) 9, 34, 117
Sse9I AATT 5 cut(s) 21, 35, 216, 278, 349
SsiI CCGC 1 cut(s) 288
SspI AATATT 1 cut(s) 251
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 337
TaiI ACGT 1 cut(s) 117
TaqI TCGA 1 cut(s) 19
TaqII GACCGA 1 cut(s) 63
TasI AATT 5 cut(s) 21, 35, 216, 278, 349
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 2 cut(s) 87, 212
Tru1I TTAA 5 cut(s) 77, 111, 189, 267, 282
Tru9I TTAA 5 cut(s) 77, 111, 189, 267, 282
TscAI CASTG 1 cut(s) 342
TseI GCWGC 2 cut(s) 141, 255
TspDTI ATGAA 1 cut(s) 254
TspRI CASTG 1 cut(s) 342
Van91I CCANNNNNTGG 1 cut(s) 68
XapI RAATTY 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.