Rh1BG328900

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0006852
Physical Location & Seq
Reverse (-)
46327089 .. 46334841
7753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG328900.1

Sequence Viewer

Length: 633 bp
ATGATACACGGCCGTGGCAAGCACCCAAAGAATGGAATGTTTAAAGGACGAATCAACCCAAGTGATGTTATCATCAATATTGGAGATACAAGTCAGACTCCACGCTGCCCCATTCCTGGACGCAGTATGTTTGACATTTCAAAATTTAACACCGCGAGGGAACTAAAGGACGAAGAAATTACTGCACCTACTGTTGGATGTTGCACGTTAATGGTGAAAAATTTTCGCTTTACTGGGAAAGCATCAGTCGACTACGAGAATGAGGTTGAAGTTGAAAACCTTGTGTTTAATTGCGTTAAAGAATTCCAGCTTGGGCCTAGCATACTGGTAATTGATGAGCGGAGGAGAGATTGCTTCCAAGAGTACAAATTAAGCAGTAAGTTATGTTTTAATGATGTTCCAGTGTGCCATCAGAATTTGAACAAGGAAGAGTTGAAGGAGAAAACAGAAGAAGATAAGCTTGTTGTTTACTGGAATGCAAACAAGGAAGTTGCTATTTTTCTAAATCATCGGAGAGAAGTGTCAGATTTTGAAGAGAAACAAGCTTGGGAGCTTAGCATACAGATCTTTGAACTAGAGGCAAGTATTATATCTCATTTTGCCAATTTTTGTCCATTCTTATCAATAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

210

Amino Acids

24.26

Weight (kDa)

5.64

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I_N PF02919 2 - 52 1.7e-10 Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I PF01028 58 - 196 8e-12 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 32
AccBSI CCGCTC 1 cut(s) 340
AccI GTMKAC 1 cut(s) 249
AccII CGCG 1 cut(s) 155
AciI CCGC 2 cut(s) 153, 340
AcoI YGGCCR 1 cut(s) 10
AcsI RAATTY 4 cut(s) 143, 220, 302, 415
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 3 cut(s) 32, 116, 194
AgsI TTSAA 7 cut(s) 141, 269, 275, 421, 436, 533, 572
AjnI CCWGG 1 cut(s) 115
AjuI GAANNNNNNNTTGG 2 cut(s) 294, 326
AleI CACNNNNGTG 1 cut(s) 12
AluBI AGCT 4 cut(s) 310, 460, 545, 553
AluI AGCT 4 cut(s) 310, 460, 545, 553
AoxI GGCC 2 cut(s) 10, 314
ApeKI GCWGC 1 cut(s) 105
ApoI RAATTY 4 cut(s) 143, 220, 302, 415
AspS9I GGNCC 1 cut(s) 314
AsuHPI GGTGA 1 cut(s) 226
BbvI GCAGC 1 cut(s) 92
BccI CCATC 1 cut(s) 417
BceAI ACGGC 1 cut(s) 25
BciT130I CCWGG 1 cut(s) 117
BfaI CTAG 2 cut(s) 318, 575
BglII AGATCT 1 cut(s) 564
BisI GCNGC 1 cut(s) 106
BlpI GCTNAGC 1 cut(s) 554
BlsI GCNGC 1 cut(s) 107
Bme1390I CCNGG 1 cut(s) 117
BmgT120I GGNCC 1 cut(s) 314
BmrFI CCNGG 1 cut(s) 117
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 1 cut(s) 251
BmuI ACTGGG 1 cut(s) 243
Bpu1102I GCTNAGC 1 cut(s) 554
BsaJI CCNNGG 1 cut(s) 13
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 116, 194
Bse1I ACTGG 4 cut(s) 238, 330, 401, 476
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 13
BseGI GGATG 1 cut(s) 203
BseLI CCNNNNNNNGG 3 cut(s) 32, 116, 194
BseNI ACTGG 4 cut(s) 238, 330, 401, 476
BseRI GAGGAG 1 cut(s) 358
BseX3I CGGCCG 1 cut(s) 10
BseXI GCAGC 1 cut(s) 92
BsgI GTGCAG 1 cut(s) 168
Bsh1236I CGCG 1 cut(s) 155
Bsh1285I CGRYCG 1 cut(s) 13
BshFI GGCC 2 cut(s) 12, 316
BsiEI CGRYCG 1 cut(s) 13
BslI CCNNNNNNNGG 3 cut(s) 32, 116, 194
BsmI GAATGC 1 cut(s) 481
BsnI GGCC 2 cut(s) 12, 316
Bsp143I GATC 1 cut(s) 564
Bsp1720I GCTNAGC 1 cut(s) 554
BspACI CCGC 2 cut(s) 153, 340
BspANI GGCC 2 cut(s) 12, 316
BspFNI CGCG 1 cut(s) 155
BsrBI CCGCTC 1 cut(s) 340
BsrI ACTGG 4 cut(s) 238, 330, 401, 476
BssECI CCNNGG 1 cut(s) 13
BssMI GATC 1 cut(s) 564
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 193
Bst6I CTCTTC 2 cut(s) 423, 528
BstC8I GCNNGC 1 cut(s) 20
BstDEI CTNAG 1 cut(s) 554
BstDSI CCRYGG 1 cut(s) 13
BstF5I GGATG 1 cut(s) 203
BstFNI CGCG 1 cut(s) 155
BstKTI GATC 1 cut(s) 567
BstMBI GATC 1 cut(s) 564
BstMCI CGRYCG 1 cut(s) 13
BstNI CCWGG 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 115
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 1 cut(s) 92
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
BstZI CGGCCG 1 cut(s) 10
BsuRI GGCC 2 cut(s) 12, 316
BtgI CCRYGG 1 cut(s) 13
BtsCI GGATG 1 cut(s) 203
BtsIMutI CAGTG 1 cut(s) 408
Cac8I GCNNGC 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 314
CseI GACGC 1 cut(s) 129
Csp6I GTAC 1 cut(s) 364
CviJI RGCY 6 cut(s) 12, 310, 316, 460, 545, 553
CviKI_1 RGCY 6 cut(s) 12, 310, 316, 460, 545, 553
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 1 cut(s) 554
DpnI GATC 1 cut(s) 566
DpnII GATC 1 cut(s) 564
DraI TTTAAA 1 cut(s) 43
EaeI YGGCCR 1 cut(s) 10
EagI CGGCCG 1 cut(s) 10
Eam1104I CTCTTC 2 cut(s) 423, 528
EarI CTCTTC 2 cut(s) 423, 528
EclXI CGGCCG 1 cut(s) 10
Eco52I CGGCCG 1 cut(s) 10
EcoRI GAATTC 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 115
FaiI YATR 5 cut(s) 128, 323, 385, 560, 590
FalI AAGNNNNNCTT 2 cut(s) 444, 476
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 1 cut(s) 106
FokI GGATG 1 cut(s) 210
Fsp4HI GCNGC 1 cut(s) 106
FspBI CTAG 2 cut(s) 318, 575
GluI GCNGC 1 cut(s) 106
HaeIII GGCC 2 cut(s) 12, 316
HgaI GACGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HindIII AAGCTT 2 cut(s) 458, 543
HinfI GANTC 2 cut(s) 51, 97
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 2 cut(s) 250, 469
Hpy188I TCNGA 4 cut(s) 96, 414, 513, 526
Hpy8I GTNNAC 2 cut(s) 250, 469
HpyAV CCTTC 1 cut(s) 430
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 3 cut(s) 185, 204, 479
HpyF3I CTNAG 1 cut(s) 554
HpySE526I ACGT 1 cut(s) 206
Kzo9I GATC 1 cut(s) 564
LmnI GCTCC 1 cut(s) 550
LpnPI CCDG 7 cut(s) 102, 129, 219, 311, 320, 414, 457
Lsp1109I GCAGC 1 cut(s) 92
LweI GCATC 1 cut(s) 251
MaeI CTAG 2 cut(s) 318, 575
MaeII ACGT 1 cut(s) 206
MalI GATC 1 cut(s) 566
MbiI CCGCTC 1 cut(s) 340
MboI GATC 1 cut(s) 564
MboII GAAGA 5 cut(s) 185, 440, 461, 464, 545
MflI RGATCY 1 cut(s) 564
MlyI GAGTC 1 cut(s) 91
MmeI TCCRAC 1 cut(s) 175
MnlI CCTC 4 cut(s) 150, 256, 336, 571
MseI TTAA 8 cut(s) 42, 147, 209, 288, 297, 371, 390, 631
MslI CAYNNNNRTG 2 cut(s) 12, 209
MspR9I CCNGG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 481
MvaI CCWGG 1 cut(s) 117
MvnI CGCG 1 cut(s) 155
NdeII GATC 1 cut(s) 564
OliI CACNNNNGTG 1 cut(s) 12
PctI GAATGC 1 cut(s) 481
PfeI GAWTC 1 cut(s) 51
PflMI CCANNNNNTGG 1 cut(s) 32
PfoI TCCNGGA 1 cut(s) 115
PkrI GCNGC 1 cut(s) 107
PleI GAGTC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 91
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspPI GGNCC 1 cut(s) 314
PsuI RGATCY 1 cut(s) 564
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 2 cut(s) 12, 209
SalI GTCGAC 1 cut(s) 248
SaqAI TTAA 8 cut(s) 42, 147, 209, 288, 297, 371, 390, 631
SatI GCNGC 1 cut(s) 106
Sau3AI GATC 1 cut(s) 564
Sau96I GGNCC 1 cut(s) 314
SchI GAGTC 1 cut(s) 91
ScrFI CCNGG 1 cut(s) 117
SetI ASST 8 cut(s) 190, 209, 267, 282, 312, 462, 547, 555
SfaNI GCATC 1 cut(s) 251
SmiMI CAYNNNNRTG 2 cut(s) 12, 209
SsiI CCGC 2 cut(s) 153, 340
SspI AATATT 1 cut(s) 79
SspMI CTAG 2 cut(s) 318, 575
StyD4I CCNGG 1 cut(s) 115
TaaI ACNGT 1 cut(s) 193
TaiI ACGT 1 cut(s) 209
TaqI TCGA 1 cut(s) 249
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 1 cut(s) 51
Tru1I TTAA 8 cut(s) 42, 147, 209, 288, 297, 371, 390, 631
Tru9I TTAA 8 cut(s) 42, 147, 209, 288, 297, 371, 390, 631
TscAI CASTG 1 cut(s) 408
TseI GCWGC 1 cut(s) 105
TspRI CASTG 1 cut(s) 408
Van91I CCANNNNNTGG 1 cut(s) 32
XapI RAATTY 4 cut(s) 143, 220, 302, 415
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 2 cut(s) 318, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.