Rh1CG342400

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0006852
Physical Location & Seq
Reverse (-)
61683232 .. 61696300
13069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG342400.1

Sequence Viewer

Length: 783 bp
ATGGAGGAAATCAAGGTTCCCATCCAAGTTCGCATTCAGGGTCAAAATTCGAATGGAATGTTTAAAGGACGAATCAACCCAAGTGATGTTATCATCAATATTGGAGATACAAGTCAGACTCCATGCTGCCCCATTCCTGGACGTTGGAAGAAAGTTGTACGCGATCCTCATCTTAAATGGCTCACATATTGGAAAGATTCAATTTTCGGGAGAAGAAAGTACATGAACTTGAAATATTGCAGCAGTATGTTTGACATTTCAAAATTTAACACCGCGAGGGAACTAAAGGACGAAGAAATTACTGCACCTACTGTTGGATGTTGCACGTTAATGGTGAAAAATTTTCGCTTTACTGGGAAAGCATCAGTCGACTACGAGAATGAGGTTGAAGTTGAAAACCTTGTGTTTAATTGCGTTAAAGAATTCCAGCTTGGGAAATCTCCTGAAGACAAGTTGTTTGACAAAAAATTGAATAGATATTTACAAAACTTGGGAAAACCTTTTGGCCTCCATTTTACAGCAAAGACATTCCGCACATTTCGAGCATCTGAAATGATGGATGATTTGTTGAACAAGGAAGAGTTGAAGGAGAAAACAGAAGAAGATAAGCTTGCTGTTTACTGGAATGCAAACAAGGAAGTTGCTATTTTTCTAAATCATCGGAGAGAAGTGTCAGATTTTGAAGAGAAACAAGCTTGGGAGCTTAGCATACAGATCTTTGAACTAGAGGCAAGTATTATATCTCATTTTGCCAATTTTTGTCCATTCTTATCAATAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

260

Amino Acids

30.39

Weight (kDa)

7.55

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I_N PF02919 17 - 76 2.4e-14 Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I PF01028 99 - 245 8.5e-31 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 369
AccII CGCG 2 cut(s) 162, 275
AciI CCGC 2 cut(s) 273, 532
AclWI GGATC 1 cut(s) 158
AcsI RAATTY 4 cut(s) 46, 263, 340, 422
AcuI CTGAAG 1 cut(s) 465
AfaI GTAC 2 cut(s) 159, 221
AfiI CCNNNNNNNGG 2 cut(s) 137, 314
AjnI CCWGG 1 cut(s) 136
AjuI GAANNNNNNNTTGG 4 cut(s) 18, 50, 414, 446
AluBI AGCT 4 cut(s) 430, 610, 695, 703
AluI AGCT 4 cut(s) 430, 610, 695, 703
AlwI GGATC 1 cut(s) 158
AoxI GGCC 1 cut(s) 505
ApeKI GCWGC 2 cut(s) 126, 240
ApoI RAATTY 4 cut(s) 46, 263, 340, 422
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
AsuHPI GGTGA 1 cut(s) 346
AsuII TTCGAA 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 453
BbvI GCAGC 2 cut(s) 113, 252
BccI CCATC 2 cut(s) 29, 550
BciT130I CCWGG 1 cut(s) 138
BfaI CTAG 1 cut(s) 725
BglII AGATCT 1 cut(s) 714
BisI GCNGC 2 cut(s) 127, 241
BlpI GCTNAGC 1 cut(s) 704
BlsI GCNGC 2 cut(s) 128, 242
Bme1390I CCNGG 1 cut(s) 138
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 1 cut(s) 138
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 2 cut(s) 371, 554
BmuI ACTGGG 1 cut(s) 363
BpiI GAAGAC 1 cut(s) 453
Bpu1102I GCTNAGC 1 cut(s) 704
Bpu14I TTCGAA 1 cut(s) 50
BsaBI GATNNNNATC 1 cut(s) 168
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 314
Bse1I ACTGG 2 cut(s) 358, 626
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 1 cut(s) 138
BseGI GGATG 3 cut(s) 21, 323, 565
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 2 cut(s) 137, 314
BseNI ACTGG 2 cut(s) 358, 626
BseXI GCAGC 2 cut(s) 113, 252
BsgI GTGCAG 1 cut(s) 288
Bsh1236I CGCG 2 cut(s) 162, 275
BshFI GGCC 1 cut(s) 507
BslI CCNNNNNNNGG 2 cut(s) 137, 314
BsmI GAATGC 2 cut(s) 33, 631
BsnI GGCC 1 cut(s) 507
Bsp119I TTCGAA 1 cut(s) 50
Bsp143I GATC 2 cut(s) 163, 714
Bsp1720I GCTNAGC 1 cut(s) 704
BspACI CCGC 2 cut(s) 273, 532
BspANI GGCC 1 cut(s) 507
BspFNI CGCG 2 cut(s) 162, 275
BspLI GGNNCC 1 cut(s) 18
BspPI GGATC 1 cut(s) 158
BspT104I TTCGAA 1 cut(s) 50
BsrI ACTGG 2 cut(s) 358, 626
BssMI GATC 2 cut(s) 163, 714
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 1 cut(s) 313
Bst6I CTCTTC 2 cut(s) 573, 678
BstBI TTCGAA 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 612
BstDEI CTNAG 1 cut(s) 704
BstF5I GGATG 3 cut(s) 21, 323, 565
BstFNI CGCG 2 cut(s) 162, 275
BstKTI GATC 2 cut(s) 166, 717
BstMBI GATC 2 cut(s) 163, 714
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstUI CGCG 2 cut(s) 162, 275
BstV1I GCAGC 2 cut(s) 113, 252
BstV2I GAAGAC 1 cut(s) 453
BstX2I RGATCY 1 cut(s) 714
BstYI RGATCY 1 cut(s) 714
BsuRI GGCC 1 cut(s) 507
BtsCI GGATG 3 cut(s) 21, 323, 565
Cac8I GCNNGC 1 cut(s) 612
Csp6I GTAC 2 cut(s) 158, 220
CviAII CATG 2 cut(s) 123, 223
CviJI RGCY 6 cut(s) 181, 430, 507, 610, 695, 703
CviKI_1 RGCY 6 cut(s) 181, 430, 507, 610, 695, 703
CviQI GTAC 2 cut(s) 158, 220
DdeI CTNAG 1 cut(s) 704
DpnI GATC 2 cut(s) 165, 716
DpnII GATC 2 cut(s) 163, 714
DraI TTTAAA 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 573, 678
EarI CTCTTC 2 cut(s) 573, 678
Eco57I CTGAAG 1 cut(s) 465
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 136
FaeI CATG 2 cut(s) 126, 226
FaiI YATR 6 cut(s) 124, 187, 224, 248, 710, 740
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FatI CATG 2 cut(s) 122, 222
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 2 cut(s) 127, 241
FokI GGATG 3 cut(s) 8, 330, 572
Fsp4HI GCNGC 2 cut(s) 127, 241
FspBI CTAG 1 cut(s) 725
GluI GCNGC 2 cut(s) 127, 241
HaeIII GGCC 1 cut(s) 507
Hin1II CATG 2 cut(s) 126, 226
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HindIII AAGCTT 2 cut(s) 608, 693
HinfI GANTC 3 cut(s) 72, 118, 197
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 2 cut(s) 370, 619
Hpy188I TCNGA 4 cut(s) 117, 550, 663, 676
Hpy188III TCNNGA 2 cut(s) 208, 443
Hpy8I GTNNAC 2 cut(s) 370, 619
HpyAV CCTTC 1 cut(s) 580
HpyCH4III ACNGT 1 cut(s) 313
HpyCH4IV ACGT 2 cut(s) 142, 326
HpyCH4V TGCA 4 cut(s) 240, 305, 324, 629
HpyF3I CTNAG 1 cut(s) 704
HpySE526I ACGT 2 cut(s) 142, 326
Hsp92II CATG 2 cut(s) 126, 226
Kzo9I GATC 2 cut(s) 163, 714
LmnI GCTCC 1 cut(s) 700
LpnPI CCDG 7 cut(s) 23, 123, 150, 339, 440, 456, 607
Lsp1109I GCAGC 2 cut(s) 113, 252
LweI GCATC 2 cut(s) 371, 554
MaeI CTAG 1 cut(s) 725
MaeII ACGT 2 cut(s) 142, 326
MalI GATC 2 cut(s) 165, 716
MboI GATC 2 cut(s) 163, 714
MboII GAAGA 8 cut(s) 160, 225, 305, 458, 590, 611, 614, 695
MflI RGATCY 1 cut(s) 714
MlyI GAGTC 1 cut(s) 112
MmeI TCCRAC 2 cut(s) 125, 295
MnlI CCTC 5 cut(s) 177, 270, 376, 518, 721
MseI TTAA 7 cut(s) 63, 174, 267, 329, 408, 417, 781
MslI CAYNNNNRTG 1 cut(s) 329
MspR9I CCNGG 1 cut(s) 138
Mva1269I GAATGC 2 cut(s) 33, 631
MvaI CCWGG 1 cut(s) 138
MvnI CGCG 2 cut(s) 162, 275
NdeII GATC 2 cut(s) 163, 714
NlaIII CATG 2 cut(s) 126, 226
NlaIV GGNNCC 1 cut(s) 18
NspV TTCGAA 1 cut(s) 50
PctI GAATGC 2 cut(s) 33, 631
PfeI GAWTC 2 cut(s) 72, 197
PfoI TCCNGGA 1 cut(s) 136
PkrI GCNGC 2 cut(s) 128, 242
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 1 cut(s) 18
PsuI RGATCY 1 cut(s) 714
RsaI GTAC 2 cut(s) 159, 221
RsaNI GTAC 2 cut(s) 158, 220
RseI CAYNNNNRTG 1 cut(s) 329
SalI GTCGAC 1 cut(s) 368
SaqAI TTAA 7 cut(s) 63, 174, 267, 329, 408, 417, 781
SatI GCNGC 2 cut(s) 127, 241
Sau3AI GATC 2 cut(s) 163, 714
SchI GAGTC 1 cut(s) 112
ScrFI CCNGG 1 cut(s) 138
SfaNI GCATC 2 cut(s) 371, 554
SfuI TTCGAA 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 329
SsiI CCGC 2 cut(s) 273, 532
SspI AATATT 2 cut(s) 100, 236
SspMI CTAG 1 cut(s) 725
StyD4I CCNGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 313
TaiI ACGT 2 cut(s) 145, 329
TaqI TCGA 3 cut(s) 50, 369, 541
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 2 cut(s) 72, 197
Tru1I TTAA 7 cut(s) 63, 174, 267, 329, 408, 417, 781
Tru9I TTAA 7 cut(s) 63, 174, 267, 329, 408, 417, 781
TseI GCWGC 2 cut(s) 126, 240
TspDTI ATGAA 1 cut(s) 239
XapI RAATTY 4 cut(s) 46, 263, 340, 422
XmiI GTMKAC 1 cut(s) 369
XspI CTAG 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.