RchiOBHm_Chr2g0094121

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006381
Physical Location & Seq
Forward (+)
7149898 .. 7150924
1027 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46914

Sequence Viewer

Length: 678 bp
ATGGGAAGAAGAAAGAAGACTCCCTCCATTTCCACCATACCAGAAGTGAAGAGCTGCCTGGATTCCAATTCCAATTCCATTTCAAATTCCAATTCCAATTCCAATTCCAATTCCAATTCCGGTGAAGCAAAATCGAAGAGCTCCATAGAGCAATTGGAATCTCAGATCCATGAGCTTCAGATCTCTACACTCTCCATCTCCACCAAGCAAACCGAGCTCCAGACCTCACAGTCTCATCTCCACAAGCTCATCGCCCTCGAAAACCTCCCTGTCTTCCACGGCCGCGCCGGCAACGAGAACGCCGTCGACTGGCTCACCGCCACCGAGCGCCACTTCGCCTACCTCGACATCCCCGCCGCCGACCGAGTCAAGATCGCCTCGACTCGCTTGCGCTGCGACGCGAGCCCCTGGATGTGCTGGTACGAGTCTCGGTACCCGGCGAGTGCGCCGTGGTCGATTTTCCGGCACGAGTTTCTGAGGTTTTTCTGGCCGGAGACGCTCATCGCCGCCGTGTGTAATTTGGAGCAGAAGAGGTCGGTGAAGGAGTATGAGGAGTTGACGAGGCATTTTGTTGGTGGATTGAAGGAGGAGATACGGCGGAAGGTGGAGTCGTTTCGGCCCAAAACCCTAGCTGACGCCATGGATTTTGCTCGCAATGTGGAGGCTGAAAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

225

Amino Acids

25.55

Weight (kDa)

7.73

Isoelectric Point (pI)

56.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 229
Acc65I GGTACC 1 cut(s) 432
AccB1I GGYRCC 1 cut(s) 432
AccI GTMKAC 1 cut(s) 306
AccII CGCG 2 cut(s) 285, 401
AciI CCGC 6 cut(s) 283, 318, 354, 357, 507, 598
AclWI GGATC 1 cut(s) 160
AcoI YGGCCR 2 cut(s) 280, 488
AcsI RAATTY 1 cut(s) 85
AcuI CTGAAG 1 cut(s) 161
AcyI GRCGYC 1 cut(s) 636
AfaI GTAC 2 cut(s) 422, 434
AfiI CCNNNNNNNGG 1 cut(s) 309
AgsI TTSAA 2 cut(s) 84, 583
AjnI CCWGG 2 cut(s) 57, 407
AluBI AGCT 6 cut(s) 54, 141, 175, 217, 247, 632
AluI AGCT 6 cut(s) 54, 141, 175, 217, 247, 632
Alw21I GWGCWC 2 cut(s) 143, 219
Alw26I GTCTC 3 cut(s) 237, 432, 488
AlwI GGATC 1 cut(s) 160
AoxI GGCC 3 cut(s) 280, 488, 617
ApeKI GCWGC 2 cut(s) 54, 393
ApoI RAATTY 1 cut(s) 85
Asp718I GGTACC 1 cut(s) 432
AspLEI GCGC 4 cut(s) 287, 330, 393, 448
AspS9I GGNCC 1 cut(s) 618
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 3 cut(s) 134, 307, 550
BanI GGYRCC 1 cut(s) 432
BanII GRGCYC 3 cut(s) 143, 219, 407
BauI CACGAG 1 cut(s) 467
BbsI GAAGAC 2 cut(s) 23, 265
Bbv12I GWGCWC 2 cut(s) 143, 219
BbvI GCAGC 2 cut(s) 41, 380
BccI CCATC 1 cut(s) 203
BceAI ACGGC 5 cut(s) 287, 295, 433, 494, 611
BcgI CGANNNNNNTGC 2 cut(s) 370, 404
BciT130I CCWGG 2 cut(s) 59, 409
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 3 cut(s) 237, 432, 488
BfaI CTAG 1 cut(s) 629
BfoI RGCGCY 1 cut(s) 331
BglI GCCNNNNNGGC 1 cut(s) 288
BglII AGATCT 1 cut(s) 180
BisI GCNGC 5 cut(s) 55, 283, 357, 394, 507
BlsI GCNGC 5 cut(s) 56, 284, 358, 395, 508
Bme1390I CCNGG 3 cut(s) 59, 409, 437
BmgT120I GGNCC 1 cut(s) 618
BmiI GGNNCC 1 cut(s) 434
BmrFI CCNGG 3 cut(s) 59, 409, 437
BpiI GAAGAC 2 cut(s) 23, 265
BpmI CTGGAG 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 437
BsaHI GRCGYC 1 cut(s) 636
BsaJI CCNNGG 4 cut(s) 277, 407, 449, 639
BsaWI WCCGGW 1 cut(s) 119
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 314
Bse3DI GCAATG 1 cut(s) 661
BseBI CCWGG 2 cut(s) 59, 409
BseDI CCNNGG 4 cut(s) 277, 407, 449, 639
BseGI GGATG 2 cut(s) 348, 417
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMI GCAATG 1 cut(s) 661
BseMII CTCAG 2 cut(s) 176, 467
BseNI ACTGG 1 cut(s) 314
BseRI GAGGAG 2 cut(s) 566, 602
BseX3I CGGCCG 1 cut(s) 280
BseXI GCAGC 2 cut(s) 41, 380
Bsh1236I CGCG 2 cut(s) 285, 401
Bsh1285I CGRYCG 2 cut(s) 283, 364
BshFI GGCC 3 cut(s) 282, 490, 619
BshNI GGYRCC 1 cut(s) 432
BsiEI CGRYCG 2 cut(s) 283, 364
BsiHKAI GWGCWC 2 cut(s) 143, 219
BsiSI CCGG 5 cut(s) 120, 288, 437, 463, 491
BslI CCNNNNNNNGG 1 cut(s) 309
BsmAI GTCTC 3 cut(s) 237, 432, 488
BsmBI CGTCTC 1 cut(s) 488
BsnI GGCC 3 cut(s) 282, 490, 619
Bsp1286I GDGCHC 3 cut(s) 143, 219, 407
Bsp143I GATC 3 cut(s) 165, 180, 372
Bsp19I CCATGG 1 cut(s) 639
BspACI CCGC 6 cut(s) 283, 318, 354, 357, 507, 598
BspANI GGCC 3 cut(s) 282, 490, 619
BspCNI CTCAG 2 cut(s) 175, 468
BspFNI CGCG 2 cut(s) 285, 401
BspLI GGNNCC 1 cut(s) 434
BspPI GGATC 1 cut(s) 160
BspQI GCTCTTC 2 cut(s) 44, 131
BspT107I GGYRCC 1 cut(s) 432
BsrDI GCAATG 1 cut(s) 661
BsrFI RCCGGY 1 cut(s) 287
BsrI ACTGG 1 cut(s) 314
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 4 cut(s) 277, 407, 449, 639
BssMI GATC 3 cut(s) 165, 180, 372
BssNI GRCGYC 1 cut(s) 636
BssSI CACGAG 1 cut(s) 467
BssT1I CCWWGG 1 cut(s) 639
Bst2BI CACGAG 1 cut(s) 467
Bst2UI CCWGG 2 cut(s) 59, 409
Bst4CI ACNGT 1 cut(s) 231
Bst6I CTCTTC 3 cut(s) 44, 131, 524
BstACI GRCGYC 1 cut(s) 636
BstC8I GCNNGC 4 cut(s) 289, 389, 403, 652
BstDEI CTNAG 2 cut(s) 162, 476
BstDSI CCRYGG 3 cut(s) 277, 449, 639
BstF5I GGATG 2 cut(s) 348, 417
BstFNI CGCG 2 cut(s) 285, 401
BstH2I RGCGCY 1 cut(s) 331
BstHHI GCGC 4 cut(s) 287, 330, 393, 448
BstKTI GATC 3 cut(s) 168, 183, 375
BstMAI GTCTC 3 cut(s) 237, 432, 488
BstMBI GATC 3 cut(s) 165, 180, 372
BstMCI CGRYCG 2 cut(s) 283, 364
BstMWI GCNNNNNNNGC 6 cut(s) 214, 288, 393, 402, 496, 671
BstNI CCWGG 2 cut(s) 59, 409
BstSCI CCNGG 3 cut(s) 57, 407, 435
BstUI CGCG 2 cut(s) 285, 401
BstV1I GCAGC 2 cut(s) 41, 380
BstV2I GAAGAC 2 cut(s) 23, 265
BstX2I RGATCY 2 cut(s) 165, 180
BstYI RGATCY 2 cut(s) 165, 180
BstZI CGGCCG 1 cut(s) 280
BsuRI GGCC 3 cut(s) 282, 490, 619
BtgI CCRYGG 3 cut(s) 277, 449, 639
BtgZI GCGATG 2 cut(s) 235, 487
BtsCI GGATG 2 cut(s) 348, 417
Cac8I GCNNGC 4 cut(s) 289, 389, 403, 652
CfoI GCGC 4 cut(s) 287, 330, 393, 448
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 1 cut(s) 618
CseI GACGC 3 cut(s) 407, 505, 644
Csp6I GTAC 2 cut(s) 421, 433
CviAII CATG 2 cut(s) 170, 640
CviQI GTAC 2 cut(s) 421, 433
DdeI CTNAG 2 cut(s) 162, 476
DpnI GATC 3 cut(s) 167, 182, 374
DpnII GATC 3 cut(s) 165, 180, 372
DrdI GACNNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 2 cut(s) 280, 488
EagI CGGCCG 1 cut(s) 280
Eam1104I CTCTTC 3 cut(s) 44, 131, 524
EarI CTCTTC 3 cut(s) 44, 131, 524
EciI GGCGGA 1 cut(s) 613
Ecl136II GAGCTC 2 cut(s) 141, 217
EclXI CGGCCG 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 639
Eco24I GRGCYC 3 cut(s) 143, 219, 407
Eco52I CGGCCG 1 cut(s) 280
Eco53kI GAGCTC 2 cut(s) 141, 217
Eco57I CTGAAG 1 cut(s) 161
EcoICRI GAGCTC 2 cut(s) 141, 217
EcoRII CCWGG 2 cut(s) 57, 407
EcoT14I CCWWGG 1 cut(s) 639
EcoT38I GRGCYC 3 cut(s) 143, 219, 407
ErhI CCWWGG 1 cut(s) 639
Esp3I CGTCTC 1 cut(s) 488
FaeI CATG 2 cut(s) 173, 643
FaiI YATR 6 cut(s) 38, 146, 171, 549, 641, 676
FatI CATG 2 cut(s) 169, 639
FauI CCCGC 1 cut(s) 361
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 5 cut(s) 55, 283, 357, 394, 507
FokI GGATG 2 cut(s) 335, 424
FriOI GRGCYC 3 cut(s) 143, 219, 407
Fsp4HI GCNGC 5 cut(s) 55, 283, 357, 394, 507
FspBI CTAG 1 cut(s) 629
GlaI GCGC 4 cut(s) 286, 329, 392, 447
GluI GCNGC 5 cut(s) 55, 283, 357, 394, 507
GsuI CTGGAG 1 cut(s) 203
HaeII RGCGCY 1 cut(s) 331
HaeIII GGCC 3 cut(s) 282, 490, 619
HapII CCGG 5 cut(s) 120, 288, 437, 463, 491
HgaI GACGC 3 cut(s) 407, 505, 644
HhaI GCGC 4 cut(s) 287, 330, 393, 448
Hin1I GRCGYC 1 cut(s) 636
Hin1II CATG 2 cut(s) 173, 643
Hin6I GCGC 4 cut(s) 285, 328, 391, 446
HinP1I GCGC 4 cut(s) 285, 328, 391, 446
HincII GTYRAC 2 cut(s) 307, 558
HindII GTYRAC 2 cut(s) 307, 558
HinfI GANTC 7 cut(s) 19, 62, 158, 366, 382, 425, 608
HpaII CCGG 5 cut(s) 120, 288, 437, 463, 491
HphI GGTGA 3 cut(s) 134, 307, 550
Hpy166II GTNNAC 2 cut(s) 307, 558
Hpy188I TCNGA 3 cut(s) 165, 180, 477
Hpy188III TCNNGA 2 cut(s) 220, 370
Hpy8I GTNNAC 2 cut(s) 307, 558
Hpy99I CGWCG 2 cut(s) 308, 401
HpyAV CCTTC 3 cut(s) 535, 577, 595
HpyCH4III ACNGT 1 cut(s) 231
HpyCH4V TGCA 1 cut(s) 674
HpyF10VI GCNNNNNNNGC 6 cut(s) 214, 288, 393, 402, 496, 671
HpyF3I CTNAG 2 cut(s) 162, 476
Hsp92I GRCGYC 1 cut(s) 636
Hsp92II CATG 2 cut(s) 173, 643
HspAI GCGC 4 cut(s) 285, 328, 391, 446
KpnI GGTACC 1 cut(s) 436
KroI GCCGGC 1 cut(s) 287
KroNI GCCGGC 1 cut(s) 289
Kzo9I GATC 3 cut(s) 165, 180, 372
LguI GCTCTTC 2 cut(s) 44, 131
LmnI GCTCC 3 cut(s) 146, 222, 523
Lsp1109I GCAGC 2 cut(s) 41, 380
MaeI CTAG 1 cut(s) 629
MalI GATC 3 cut(s) 167, 182, 374
MboI GATC 3 cut(s) 165, 180, 372
MboII GAAGA 7 cut(s) 18, 21, 28, 61, 148, 265, 541
MfeI CAATTG 1 cut(s) 152
MflI RGATCY 2 cut(s) 165, 180
MhlI GDGCHC 3 cut(s) 143, 219, 407
MlyI GAGTC 5 cut(s) 13, 375, 376, 434, 617
MroNI GCCGGC 1 cut(s) 287
MspI CCGG 5 cut(s) 120, 288, 437, 463, 491
MspR9I CCNGG 3 cut(s) 59, 409, 437
MunI CAATTG 1 cut(s) 152
MvaI CCWGG 2 cut(s) 59, 409
MvnI CGCG 2 cut(s) 285, 401
MwoI GCNNNNNNNGC 6 cut(s) 214, 288, 393, 402, 496, 671
NaeI GCCGGC 1 cut(s) 289
NciI CCSGG 1 cut(s) 437
NcoI CCATGG 1 cut(s) 639
NdeII GATC 3 cut(s) 165, 180, 372
NgoMIV GCCGGC 1 cut(s) 287
NlaIII CATG 2 cut(s) 173, 643
NlaIV GGNNCC 1 cut(s) 434
PciSI GCTCTTC 2 cut(s) 44, 131
PcsI WCGNNNNNNNCGW 2 cut(s) 300, 342
PdiI GCCGGC 1 cut(s) 289
PfeI GAWTC 2 cut(s) 62, 158
PflFI GACNNNGTC 1 cut(s) 365
PkrI GCNGC 5 cut(s) 56, 284, 358, 395, 508
PleI GAGTC 5 cut(s) 13, 374, 376, 433, 616
PpsI GAGTC 5 cut(s) 13, 374, 376, 433, 616
Psp124BI GAGCTC 2 cut(s) 143, 219
Psp6I CCWGG 2 cut(s) 57, 407
PspGI CCWGG 2 cut(s) 57, 407
PspN4I GGNNCC 1 cut(s) 434
PspPI GGNCC 1 cut(s) 618
PsuI RGATCY 2 cut(s) 165, 180
PsyI GACNNNGTC 1 cut(s) 365
RsaI GTAC 2 cut(s) 422, 434
RsaNI GTAC 2 cut(s) 421, 433
SacI GAGCTC 2 cut(s) 143, 219
SalI GTCGAC 1 cut(s) 305
SapI GCTCTTC 2 cut(s) 44, 131
SatI GCNGC 5 cut(s) 55, 283, 357, 394, 507
Sau3AI GATC 3 cut(s) 165, 180, 372
Sau96I GGNCC 1 cut(s) 618
SchI GAGTC 5 cut(s) 13, 375, 376, 434, 617
ScrFI CCNGG 3 cut(s) 59, 409, 437
SduI GDGCHC 3 cut(s) 143, 219, 407
SsiI CCGC 6 cut(s) 283, 318, 354, 357, 507, 598
SspMI CTAG 1 cut(s) 629
SstI GAGCTC 2 cut(s) 143, 219
StyD4I CCNGG 3 cut(s) 57, 407, 435
StyI CCWWGG 1 cut(s) 639
TaaI ACNGT 1 cut(s) 231
TaqI TCGA 6 cut(s) 134, 258, 306, 345, 380, 455
TaqII GACCGA 1 cut(s) 378
TauI GCSGC 3 cut(s) 285, 359, 509
TfiI GAWTC 2 cut(s) 62, 158
TseI GCWGC 2 cut(s) 54, 393
Tth111I GACNNNGTC 1 cut(s) 365
XapI RAATTY 1 cut(s) 85
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.