Rh2BG088800

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006381
Physical Location & Seq
Forward (+)
6927304 .. 6928423
1120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG088800.1

Sequence Viewer

Length: 726 bp
ATGGGAAGAAGAAAGAAGACTCCCTCCATTTCCACCATACCAGAAGTGAAGAGCTGCCTGGATTCCAATTCCAATTCCATTTCAAATTCCAATTCCAATTCCAATTCCGGTGAAGCAAAATCGAAGAGCTCCATAGAGCAATTGGAATCTCAGATCCATGAGCTTCAGATCTCCACACTCTCCATCTCCACCAAGCAAACCGAGCTCCAGACCTCACAGTCTCATCTCCACAAGCTCATCGCCCTCGAAAACCTCCCCGTCTTCCACGGCCGCGCCGGCGACGAGAATGCCGTCGACTGGCTCACCGCCACCGAGCGCCACTTCGCCTACCTCGACATCCCCGCCGCCGACCGAGTCAAGATCGCCGCGACTCGCTTGCGCTGCGACGCGAGCCCCTGGATGTGCTGGTACGAGTCTCGGTACCCGGCGAGTGCGCCGTGGTCGATTTTCCGGCACGAGTTTCTGAGGTTTTTCCGGCCGGAGACGCTCATCGCCGCCGTGTGTAATTTGGAGCAGAAGAGGTCGGTGAAGGAGTATGAGGTGGAGTTTCTGAGGCTGGCTGCCGGAGGTGGGGATCCGTCGCCGGAGTGGAGCGATGAGGAGTTGACGAGGCATTTTGTTGGTGGATTGAAGGAGGAGATACGGCGGAAGGTGGAGTCGTTTCGGCCCAAAACCCTAGCTGACGCCATGGATTTTGCTCGCAATGTGGAGGCTGAAAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

241

Amino Acids

27.21

Weight (kDa)

6.2

Isoelectric Point (pI)

58.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 109 - 235 1.7e-08 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 120 - 212 1.5e-07 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 217
Acc65I GGTACC 1 cut(s) 420
AccB1I GGYRCC 1 cut(s) 420
AccI GTMKAC 1 cut(s) 294
AccII CGCG 3 cut(s) 273, 368, 389
AciI CCGC 7 cut(s) 271, 306, 342, 345, 366, 495, 646
AclWI GGATC 3 cut(s) 148, 569, 582
AcoI YGGCCR 2 cut(s) 268, 476
AcsI RAATTY 1 cut(s) 85
AcuI CTGAAG 1 cut(s) 149
AcyI GRCGYC 1 cut(s) 684
AfaI GTAC 2 cut(s) 410, 422
AfiI CCNNNNNNNGG 2 cut(s) 297, 570
AgsI TTSAA 2 cut(s) 84, 631
AjnI CCWGG 2 cut(s) 57, 395
AluBI AGCT 6 cut(s) 54, 129, 163, 205, 235, 680
AluI AGCT 6 cut(s) 54, 129, 163, 205, 235, 680
Alw21I GWGCWC 2 cut(s) 131, 207
Alw26I GTCTC 3 cut(s) 225, 420, 476
AlwI GGATC 3 cut(s) 148, 569, 582
AoxI GGCC 3 cut(s) 268, 476, 665
ApeKI GCWGC 3 cut(s) 54, 381, 560
ApoI RAATTY 1 cut(s) 85
Asp718I GGTACC 1 cut(s) 420
AspLEI GCGC 4 cut(s) 275, 318, 381, 436
AspS9I GGNCC 1 cut(s) 666
AsuC2I CCSGG 1 cut(s) 425
AsuHPI GGTGA 3 cut(s) 122, 295, 538
BamHI GGATCC 1 cut(s) 574
BanI GGYRCC 1 cut(s) 420
BanII GRGCYC 3 cut(s) 131, 207, 395
BauI CACGAG 1 cut(s) 455
BbsI GAAGAC 2 cut(s) 23, 253
Bbv12I GWGCWC 2 cut(s) 131, 207
BbvI GCAGC 3 cut(s) 41, 368, 547
BccI CCATC 1 cut(s) 191
BceAI ACGGC 5 cut(s) 275, 283, 421, 482, 659
BcgI CGANNNNNNTGC 4 cut(s) 269, 303, 358, 392
BciT130I CCWGG 2 cut(s) 59, 397
BcnI CCSGG 1 cut(s) 425
BcoDI GTCTC 3 cut(s) 225, 420, 476
BfaI CTAG 1 cut(s) 677
BfoI RGCGCY 1 cut(s) 319
BglI GCCNNNNNGGC 1 cut(s) 276
BglII AGATCT 1 cut(s) 168
BisI GCNGC 7 cut(s) 55, 271, 345, 366, 382, 495, 561
BlsI GCNGC 7 cut(s) 56, 272, 346, 367, 383, 496, 562
Bme1390I CCNGG 3 cut(s) 59, 397, 425
BmgT120I GGNCC 1 cut(s) 666
BmiI GGNNCC 2 cut(s) 422, 576
BmrFI CCNGG 3 cut(s) 59, 397, 425
BpiI GAAGAC 2 cut(s) 23, 253
BpmI CTGGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 425
BsaHI GRCGYC 1 cut(s) 684
BsaJI CCNNGG 4 cut(s) 265, 395, 437, 687
BsaWI WCCGGW 1 cut(s) 107
Bsc4I CCNNNNNNNGG 2 cut(s) 297, 570
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 302
Bse3DI GCAATG 1 cut(s) 709
BseBI CCWGG 2 cut(s) 59, 397
BseDI CCNNGG 4 cut(s) 265, 395, 437, 687
BseGI GGATG 2 cut(s) 336, 405
BseLI CCNNNNNNNGG 2 cut(s) 297, 570
BseMI GCAATG 1 cut(s) 709
BseMII CTCAG 3 cut(s) 164, 455, 542
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 2 cut(s) 614, 650
BseX3I CGGCCG 2 cut(s) 268, 476
BseXI GCAGC 3 cut(s) 41, 368, 547
Bsh1236I CGCG 3 cut(s) 273, 368, 389
Bsh1285I CGRYCG 3 cut(s) 271, 352, 479
BshFI GGCC 3 cut(s) 270, 478, 667
BshNI GGYRCC 1 cut(s) 420
BsiEI CGRYCG 3 cut(s) 271, 352, 479
BsiHKAI GWGCWC 2 cut(s) 131, 207
BsiSI CCGG 8 cut(s) 108, 276, 425, 451, 475, 479, 564, 584
BslI CCNNNNNNNGG 2 cut(s) 297, 570
BsmAI GTCTC 3 cut(s) 225, 420, 476
BsmBI CGTCTC 1 cut(s) 476
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 3 cut(s) 270, 478, 667
Bsp1286I GDGCHC 3 cut(s) 131, 207, 395
Bsp143I GATC 4 cut(s) 153, 168, 360, 574
Bsp19I CCATGG 1 cut(s) 687
BspACI CCGC 7 cut(s) 271, 306, 342, 345, 366, 495, 646
BspANI GGCC 3 cut(s) 270, 478, 667
BspCNI CTCAG 3 cut(s) 163, 456, 543
BspFNI CGCG 3 cut(s) 273, 368, 389
BspLI GGNNCC 2 cut(s) 422, 576
BspPI GGATC 3 cut(s) 148, 569, 582
BspQI GCTCTTC 2 cut(s) 44, 119
BspT107I GGYRCC 1 cut(s) 420
BsrDI GCAATG 1 cut(s) 709
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 4 cut(s) 265, 395, 437, 687
BssMI GATC 4 cut(s) 153, 168, 360, 574
BssNI GRCGYC 1 cut(s) 684
BssSI CACGAG 1 cut(s) 455
BssT1I CCWWGG 1 cut(s) 687
Bst2BI CACGAG 1 cut(s) 455
Bst2UI CCWGG 2 cut(s) 59, 397
Bst4CI ACNGT 1 cut(s) 219
Bst6I CTCTTC 3 cut(s) 44, 119, 512
BstACI GRCGYC 1 cut(s) 684
BstC8I GCNNGC 5 cut(s) 277, 377, 391, 558, 700
BstDEI CTNAG 3 cut(s) 150, 464, 551
BstDSI CCRYGG 3 cut(s) 265, 437, 687
BstF5I GGATG 2 cut(s) 336, 405
BstFNI CGCG 3 cut(s) 273, 368, 389
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 4 cut(s) 275, 318, 381, 436
BstKTI GATC 4 cut(s) 156, 171, 363, 577
BstMAI GTCTC 3 cut(s) 225, 420, 476
BstMBI GATC 4 cut(s) 153, 168, 360, 574
BstMCI CGRYCG 3 cut(s) 271, 352, 479
BstMWI GCNNNNNNNGC 6 cut(s) 202, 276, 381, 390, 484, 719
BstNI CCWGG 2 cut(s) 59, 397
BstSCI CCNGG 3 cut(s) 57, 395, 423
BstUI CGCG 3 cut(s) 273, 368, 389
BstV1I GCAGC 3 cut(s) 41, 368, 547
BstV2I GAAGAC 2 cut(s) 23, 253
BstX2I RGATCY 3 cut(s) 153, 168, 574
BstYI RGATCY 3 cut(s) 153, 168, 574
BstZI CGGCCG 2 cut(s) 268, 476
BsuRI GGCC 3 cut(s) 270, 478, 667
BtgI CCRYGG 3 cut(s) 265, 437, 687
BtgZI GCGATG 3 cut(s) 223, 475, 609
BtsCI GGATG 2 cut(s) 336, 405
Cac8I GCNNGC 5 cut(s) 277, 377, 391, 558, 700
CfoI GCGC 4 cut(s) 275, 318, 381, 436
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 666
CseI GACGC 3 cut(s) 395, 493, 692
Csp6I GTAC 2 cut(s) 409, 421
CviAII CATG 2 cut(s) 158, 688
CviQI GTAC 2 cut(s) 409, 421
DdeI CTNAG 3 cut(s) 150, 464, 551
DpnI GATC 4 cut(s) 155, 170, 362, 576
DpnII GATC 4 cut(s) 153, 168, 360, 574
DrdI GACNNNNNNGTC 1 cut(s) 217
DseDI GACNNNNNNGTC 1 cut(s) 217
EaeI YGGCCR 2 cut(s) 268, 476
EagI CGGCCG 2 cut(s) 268, 476
Eam1104I CTCTTC 3 cut(s) 44, 119, 512
EarI CTCTTC 3 cut(s) 44, 119, 512
EciI GGCGGA 1 cut(s) 661
Ecl136II GAGCTC 2 cut(s) 129, 205
EclXI CGGCCG 2 cut(s) 268, 476
Eco130I CCWWGG 1 cut(s) 687
Eco24I GRGCYC 3 cut(s) 131, 207, 395
Eco52I CGGCCG 2 cut(s) 268, 476
Eco53kI GAGCTC 2 cut(s) 129, 205
Eco57I CTGAAG 1 cut(s) 149
EcoICRI GAGCTC 2 cut(s) 129, 205
EcoRII CCWGG 2 cut(s) 57, 395
EcoT14I CCWWGG 1 cut(s) 687
EcoT38I GRGCYC 3 cut(s) 131, 207, 395
ErhI CCWWGG 1 cut(s) 687
Esp3I CGTCTC 1 cut(s) 476
FaeI CATG 2 cut(s) 161, 691
FaiI YATR 6 cut(s) 38, 134, 159, 537, 689, 724
FatI CATG 2 cut(s) 157, 687
FauI CCCGC 1 cut(s) 349
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 7 cut(s) 55, 271, 345, 366, 382, 495, 561
FokI GGATG 2 cut(s) 323, 412
FriOI GRGCYC 3 cut(s) 131, 207, 395
Fsp4HI GCNGC 7 cut(s) 55, 271, 345, 366, 382, 495, 561
FspBI CTAG 1 cut(s) 677
GlaI GCGC 4 cut(s) 274, 317, 380, 435
GluI GCNGC 7 cut(s) 55, 271, 345, 366, 382, 495, 561
GsuI CTGGAG 1 cut(s) 191
HaeII RGCGCY 1 cut(s) 319
HaeIII GGCC 3 cut(s) 270, 478, 667
HapII CCGG 8 cut(s) 108, 276, 425, 451, 475, 479, 564, 584
HgaI GACGC 3 cut(s) 395, 493, 692
HhaI GCGC 4 cut(s) 275, 318, 381, 436
Hin1I GRCGYC 1 cut(s) 684
Hin1II CATG 2 cut(s) 161, 691
Hin6I GCGC 4 cut(s) 273, 316, 379, 434
HinP1I GCGC 4 cut(s) 273, 316, 379, 434
HincII GTYRAC 2 cut(s) 295, 606
HindII GTYRAC 2 cut(s) 295, 606
HinfI GANTC 7 cut(s) 19, 62, 146, 354, 370, 413, 656
HpaII CCGG 8 cut(s) 108, 276, 425, 451, 475, 479, 564, 584
HphI GGTGA 3 cut(s) 122, 295, 538
Hpy166II GTNNAC 2 cut(s) 295, 606
Hpy188I TCNGA 4 cut(s) 153, 168, 465, 552
Hpy188III TCNNGA 2 cut(s) 208, 358
Hpy8I GTNNAC 2 cut(s) 295, 606
Hpy99I CGWCG 4 cut(s) 284, 296, 389, 583
HpyAV CCTTC 3 cut(s) 523, 625, 643
HpyCH4III ACNGT 1 cut(s) 219
HpyCH4V TGCA 1 cut(s) 722
HpyF10VI GCNNNNNNNGC 6 cut(s) 202, 276, 381, 390, 484, 719
HpyF3I CTNAG 3 cut(s) 150, 464, 551
Hsp92I GRCGYC 1 cut(s) 684
Hsp92II CATG 2 cut(s) 161, 691
HspAI GCGC 4 cut(s) 273, 316, 379, 434
KpnI GGTACC 1 cut(s) 424
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
Kzo9I GATC 4 cut(s) 153, 168, 360, 574
LguI GCTCTTC 2 cut(s) 44, 119
LmnI GCTCC 4 cut(s) 134, 210, 511, 591
Lsp1109I GCAGC 3 cut(s) 41, 368, 547
MaeI CTAG 1 cut(s) 677
MalI GATC 4 cut(s) 155, 170, 362, 576
MboI GATC 4 cut(s) 153, 168, 360, 574
MboII GAAGA 7 cut(s) 18, 21, 28, 61, 136, 253, 529
MfeI CAATTG 1 cut(s) 140
MflI RGATCY 3 cut(s) 153, 168, 574
MhlI GDGCHC 3 cut(s) 131, 207, 395
MluCI AATT 8 cut(s) 67, 73, 85, 91, 97, 103, 140, 505
MlyI GAGTC 5 cut(s) 13, 363, 364, 422, 665
MreI CGCCGGCG 1 cut(s) 275
MroNI GCCGGC 1 cut(s) 275
MspI CCGG 8 cut(s) 108, 276, 425, 451, 475, 479, 564, 584
MspR9I CCNGG 3 cut(s) 59, 397, 425
MunI CAATTG 1 cut(s) 140
Mva1269I GAATGC 1 cut(s) 292
MvaI CCWGG 2 cut(s) 59, 397
MvnI CGCG 3 cut(s) 273, 368, 389
MwoI GCNNNNNNNGC 6 cut(s) 202, 276, 381, 390, 484, 719
NaeI GCCGGC 1 cut(s) 277
NciI CCSGG 1 cut(s) 425
NcoI CCATGG 1 cut(s) 687
NdeII GATC 4 cut(s) 153, 168, 360, 574
NgoMIV GCCGGC 1 cut(s) 275
NlaIII CATG 2 cut(s) 161, 691
NlaIV GGNNCC 2 cut(s) 422, 576
PciSI GCTCTTC 2 cut(s) 44, 119
PcsI WCGNNNNNNNCGW 2 cut(s) 288, 330
PctI GAATGC 1 cut(s) 292
PdiI GCCGGC 1 cut(s) 277
PfeI GAWTC 2 cut(s) 62, 146
PflFI GACNNNGTC 1 cut(s) 353
PkrI GCNGC 7 cut(s) 56, 272, 346, 367, 383, 496, 562
PleI GAGTC 5 cut(s) 13, 362, 364, 421, 664
PpsI GAGTC 5 cut(s) 13, 362, 364, 421, 664
Psp124BI GAGCTC 2 cut(s) 131, 207
Psp6I CCWGG 2 cut(s) 57, 395
PspGI CCWGG 2 cut(s) 57, 395
PspN4I GGNNCC 2 cut(s) 422, 576
PspPI GGNCC 1 cut(s) 666
PsuI RGATCY 3 cut(s) 153, 168, 574
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 2 cut(s) 410, 422
RsaNI GTAC 2 cut(s) 409, 421
SacI GAGCTC 2 cut(s) 131, 207
SalI GTCGAC 1 cut(s) 293
SapI GCTCTTC 2 cut(s) 44, 119
SatI GCNGC 7 cut(s) 55, 271, 345, 366, 382, 495, 561
Sau3AI GATC 4 cut(s) 153, 168, 360, 574
Sau96I GGNCC 1 cut(s) 666
SchI GAGTC 5 cut(s) 13, 363, 364, 422, 665
ScrFI CCNGG 3 cut(s) 59, 397, 425
SduI GDGCHC 3 cut(s) 131, 207, 395
SgrAI CRCCGGYG 1 cut(s) 275
Sse9I AATT 8 cut(s) 67, 73, 85, 91, 97, 103, 140, 505
SsiI CCGC 7 cut(s) 271, 306, 342, 345, 366, 495, 646
SspMI CTAG 1 cut(s) 677
SstI GAGCTC 2 cut(s) 131, 207
StyD4I CCNGG 3 cut(s) 57, 395, 423
StyI CCWWGG 1 cut(s) 687
TaaI ACNGT 1 cut(s) 219
TaqI TCGA 5 cut(s) 122, 246, 294, 333, 443
TaqII GACCGA 1 cut(s) 366
TasI AATT 8 cut(s) 67, 73, 85, 91, 97, 103, 140, 505
TauI GCSGC 4 cut(s) 273, 347, 368, 497
TfiI GAWTC 2 cut(s) 62, 146
TseI GCWGC 3 cut(s) 54, 381, 560
TspGWI ACGGA 1 cut(s) 567
Tth111I GACNNNGTC 1 cut(s) 353
XapI RAATTY 1 cut(s) 85
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 294
XspI CTAG 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.