Rh2DG087800

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006381
Physical Location & Seq
Forward (+)
6805727 .. 6807062
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG087800.1

Sequence Viewer

Length: 732 bp
ATGGGAAGAAGAAAGAAGACTCCCTCCATTTCCACCATACCAGAAGTGAAGAGCTGCCTGGATTCCAATTCCAATTCCATTTCAAATTCCAATTCCAATTCCAATTCCAATTCCGGTGAAGCAAAATCGAAGAGCTCCATAGAGCAATTGGAATCTCAGATCCATGAGCTTCAGATCTCTACACTCTCCATCTCCACCAAGCAAACCGAGCTCCAGACCTCACAGTCTCATCTCCACAAGCTCATCGCCCTCGAAAACCTCCCTGTCTTCCACGGCCGCGCCGGCAACGAGAACGCCGTCGACTGGCTCACCGCCACCGAGCGCCACTTCGCCTACCTCGACATCCCCGCCGCCGACCGAGTCAAGATCGCCTCGACTCGCTTGCGCTGCGACGCGAGCCCCTGGATGTGCTGGTACGAGTCTCGGTACCCGGCGAGTGCGCCGTGGTCGATTTTCCGGCACGAGTTTCTGAGGTTTTTCTGGCCGGAGACGCTCATCGCCGCCGTGTGTAATTTGGAGCAGAAGAGGTCGGTGAAGGAGTATGAGGTGGAGTTTCTGAGGCTGGCTGCCGGAGGTGGGGATCCGTCGCCGGAGTGGAGCGATGAGGAGTTGACGAGGCATTTTGTTGGTGGATTGAAGGAGGAGATACGGCGGAAGGTGGAGTCGTTTCGGCCCAAAACCCTAGCTGACGCCATGGATTTTGCTCGCAATGTGGAGGCTGAAAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

243

Amino Acids

27.46

Weight (kDa)

6.19

Isoelectric Point (pI)

57.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 112 - 237 1.2e-07 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 122 - 214 3.3e-07 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 223
Acc65I GGTACC 1 cut(s) 426
AccB1I GGYRCC 1 cut(s) 426
AccI GTMKAC 1 cut(s) 300
AccII CGCG 2 cut(s) 279, 395
AciI CCGC 6 cut(s) 277, 312, 348, 351, 501, 652
AclWI GGATC 3 cut(s) 154, 575, 588
AcoI YGGCCR 2 cut(s) 274, 482
AcsI RAATTY 1 cut(s) 85
AcuI CTGAAG 1 cut(s) 155
AcyI GRCGYC 1 cut(s) 690
AfaI GTAC 2 cut(s) 416, 428
AfiI CCNNNNNNNGG 2 cut(s) 303, 576
AgsI TTSAA 2 cut(s) 84, 637
AjnI CCWGG 2 cut(s) 57, 401
AluBI AGCT 6 cut(s) 54, 135, 169, 211, 241, 686
AluI AGCT 6 cut(s) 54, 135, 169, 211, 241, 686
Alw21I GWGCWC 2 cut(s) 137, 213
Alw26I GTCTC 3 cut(s) 231, 426, 482
AlwI GGATC 3 cut(s) 154, 575, 588
AoxI GGCC 3 cut(s) 274, 482, 671
ApeKI GCWGC 3 cut(s) 54, 387, 566
ApoI RAATTY 1 cut(s) 85
Asp718I GGTACC 1 cut(s) 426
AspLEI GCGC 4 cut(s) 281, 324, 387, 442
AspS9I GGNCC 1 cut(s) 672
AsuC2I CCSGG 1 cut(s) 431
AsuHPI GGTGA 3 cut(s) 128, 301, 544
BamHI GGATCC 1 cut(s) 580
BanI GGYRCC 1 cut(s) 426
BanII GRGCYC 3 cut(s) 137, 213, 401
BauI CACGAG 1 cut(s) 461
BbsI GAAGAC 2 cut(s) 23, 259
Bbv12I GWGCWC 2 cut(s) 137, 213
BbvI GCAGC 3 cut(s) 41, 374, 553
BccI CCATC 1 cut(s) 197
BceAI ACGGC 5 cut(s) 281, 289, 427, 488, 665
BcgI CGANNNNNNTGC 2 cut(s) 364, 398
BciT130I CCWGG 2 cut(s) 59, 403
BcnI CCSGG 1 cut(s) 431
BcoDI GTCTC 3 cut(s) 231, 426, 482
BfaI CTAG 1 cut(s) 683
BfoI RGCGCY 1 cut(s) 325
BglI GCCNNNNNGGC 1 cut(s) 282
BglII AGATCT 1 cut(s) 174
BisI GCNGC 6 cut(s) 55, 277, 351, 388, 501, 567
BlsI GCNGC 6 cut(s) 56, 278, 352, 389, 502, 568
Bme1390I CCNGG 3 cut(s) 59, 403, 431
BmgT120I GGNCC 1 cut(s) 672
BmiI GGNNCC 2 cut(s) 428, 582
BmrFI CCNGG 3 cut(s) 59, 403, 431
BpiI GAAGAC 2 cut(s) 23, 259
BpmI CTGGAG 1 cut(s) 197
BpuMI CCSGG 1 cut(s) 431
BsaHI GRCGYC 1 cut(s) 690
BsaJI CCNNGG 4 cut(s) 271, 401, 443, 693
BsaWI WCCGGW 1 cut(s) 113
Bsc4I CCNNNNNNNGG 2 cut(s) 303, 576
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 308
Bse3DI GCAATG 1 cut(s) 715
BseBI CCWGG 2 cut(s) 59, 403
BseDI CCNNGG 4 cut(s) 271, 401, 443, 693
BseGI GGATG 2 cut(s) 342, 411
BseLI CCNNNNNNNGG 2 cut(s) 303, 576
BseMI GCAATG 1 cut(s) 715
BseMII CTCAG 3 cut(s) 170, 461, 548
BseNI ACTGG 1 cut(s) 308
BseRI GAGGAG 2 cut(s) 620, 656
BseX3I CGGCCG 1 cut(s) 274
BseXI GCAGC 3 cut(s) 41, 374, 553
Bsh1236I CGCG 2 cut(s) 279, 395
Bsh1285I CGRYCG 2 cut(s) 277, 358
BshFI GGCC 3 cut(s) 276, 484, 673
BshNI GGYRCC 1 cut(s) 426
BsiEI CGRYCG 2 cut(s) 277, 358
BsiHKAI GWGCWC 2 cut(s) 137, 213
BsiSI CCGG 7 cut(s) 114, 282, 431, 457, 485, 570, 590
BslI CCNNNNNNNGG 2 cut(s) 303, 576
BsmAI GTCTC 3 cut(s) 231, 426, 482
BsmBI CGTCTC 1 cut(s) 482
BsnI GGCC 3 cut(s) 276, 484, 673
Bsp1286I GDGCHC 3 cut(s) 137, 213, 401
Bsp143I GATC 4 cut(s) 159, 174, 366, 580
Bsp19I CCATGG 1 cut(s) 693
BspACI CCGC 6 cut(s) 277, 312, 348, 351, 501, 652
BspANI GGCC 3 cut(s) 276, 484, 673
BspCNI CTCAG 3 cut(s) 169, 462, 549
BspFNI CGCG 2 cut(s) 279, 395
BspLI GGNNCC 2 cut(s) 428, 582
BspPI GGATC 3 cut(s) 154, 575, 588
BspQI GCTCTTC 2 cut(s) 44, 125
BspT107I GGYRCC 1 cut(s) 426
BsrDI GCAATG 1 cut(s) 715
BsrFI RCCGGY 1 cut(s) 281
BsrI ACTGG 1 cut(s) 308
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 4 cut(s) 271, 401, 443, 693
BssMI GATC 4 cut(s) 159, 174, 366, 580
BssNI GRCGYC 1 cut(s) 690
BssSI CACGAG 1 cut(s) 461
BssT1I CCWWGG 1 cut(s) 693
Bst2BI CACGAG 1 cut(s) 461
Bst2UI CCWGG 2 cut(s) 59, 403
Bst4CI ACNGT 1 cut(s) 225
Bst6I CTCTTC 3 cut(s) 44, 125, 518
BstACI GRCGYC 1 cut(s) 690
BstC8I GCNNGC 5 cut(s) 283, 383, 397, 564, 706
BstDEI CTNAG 3 cut(s) 156, 470, 557
BstDSI CCRYGG 3 cut(s) 271, 443, 693
BstF5I GGATG 2 cut(s) 342, 411
BstFNI CGCG 2 cut(s) 279, 395
BstH2I RGCGCY 1 cut(s) 325
BstHHI GCGC 4 cut(s) 281, 324, 387, 442
BstKTI GATC 4 cut(s) 162, 177, 369, 583
BstMAI GTCTC 3 cut(s) 231, 426, 482
BstMBI GATC 4 cut(s) 159, 174, 366, 580
BstMCI CGRYCG 2 cut(s) 277, 358
BstMWI GCNNNNNNNGC 6 cut(s) 208, 282, 387, 396, 490, 725
BstNI CCWGG 2 cut(s) 59, 403
BstSCI CCNGG 3 cut(s) 57, 401, 429
BstUI CGCG 2 cut(s) 279, 395
BstV1I GCAGC 3 cut(s) 41, 374, 553
BstV2I GAAGAC 2 cut(s) 23, 259
BstX2I RGATCY 3 cut(s) 159, 174, 580
BstYI RGATCY 3 cut(s) 159, 174, 580
BstZI CGGCCG 1 cut(s) 274
BsuRI GGCC 3 cut(s) 276, 484, 673
BtgI CCRYGG 3 cut(s) 271, 443, 693
BtgZI GCGATG 3 cut(s) 229, 481, 615
BtsCI GGATG 2 cut(s) 342, 411
Cac8I GCNNGC 5 cut(s) 283, 383, 397, 564, 706
CfoI GCGC 4 cut(s) 281, 324, 387, 442
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 1 cut(s) 672
CseI GACGC 3 cut(s) 401, 499, 698
Csp6I GTAC 2 cut(s) 415, 427
CviAII CATG 2 cut(s) 164, 694
CviQI GTAC 2 cut(s) 415, 427
DdeI CTNAG 3 cut(s) 156, 470, 557
DpnI GATC 4 cut(s) 161, 176, 368, 582
DpnII GATC 4 cut(s) 159, 174, 366, 580
DrdI GACNNNNNNGTC 1 cut(s) 223
DseDI GACNNNNNNGTC 1 cut(s) 223
EaeI YGGCCR 2 cut(s) 274, 482
EagI CGGCCG 1 cut(s) 274
Eam1104I CTCTTC 3 cut(s) 44, 125, 518
EarI CTCTTC 3 cut(s) 44, 125, 518
EciI GGCGGA 1 cut(s) 667
Ecl136II GAGCTC 2 cut(s) 135, 211
EclXI CGGCCG 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 693
Eco24I GRGCYC 3 cut(s) 137, 213, 401
Eco52I CGGCCG 1 cut(s) 274
Eco53kI GAGCTC 2 cut(s) 135, 211
Eco57I CTGAAG 1 cut(s) 155
EcoICRI GAGCTC 2 cut(s) 135, 211
EcoRII CCWGG 2 cut(s) 57, 401
EcoT14I CCWWGG 1 cut(s) 693
EcoT38I GRGCYC 3 cut(s) 137, 213, 401
ErhI CCWWGG 1 cut(s) 693
Esp3I CGTCTC 1 cut(s) 482
FaeI CATG 2 cut(s) 167, 697
FaiI YATR 6 cut(s) 38, 140, 165, 543, 695, 730
FatI CATG 2 cut(s) 163, 693
FauI CCCGC 1 cut(s) 355
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 6 cut(s) 55, 277, 351, 388, 501, 567
FokI GGATG 2 cut(s) 329, 418
FriOI GRGCYC 3 cut(s) 137, 213, 401
Fsp4HI GCNGC 6 cut(s) 55, 277, 351, 388, 501, 567
FspBI CTAG 1 cut(s) 683
GlaI GCGC 4 cut(s) 280, 323, 386, 441
GluI GCNGC 6 cut(s) 55, 277, 351, 388, 501, 567
GsuI CTGGAG 1 cut(s) 197
HaeII RGCGCY 1 cut(s) 325
HaeIII GGCC 3 cut(s) 276, 484, 673
HapII CCGG 7 cut(s) 114, 282, 431, 457, 485, 570, 590
HgaI GACGC 3 cut(s) 401, 499, 698
HhaI GCGC 4 cut(s) 281, 324, 387, 442
Hin1I GRCGYC 1 cut(s) 690
Hin1II CATG 2 cut(s) 167, 697
Hin6I GCGC 4 cut(s) 279, 322, 385, 440
HinP1I GCGC 4 cut(s) 279, 322, 385, 440
HincII GTYRAC 2 cut(s) 301, 612
HindII GTYRAC 2 cut(s) 301, 612
HinfI GANTC 7 cut(s) 19, 62, 152, 360, 376, 419, 662
HpaII CCGG 7 cut(s) 114, 282, 431, 457, 485, 570, 590
HphI GGTGA 3 cut(s) 128, 301, 544
Hpy166II GTNNAC 2 cut(s) 301, 612
Hpy188I TCNGA 4 cut(s) 159, 174, 471, 558
Hpy188III TCNNGA 2 cut(s) 214, 364
Hpy8I GTNNAC 2 cut(s) 301, 612
Hpy99I CGWCG 3 cut(s) 302, 395, 589
HpyAV CCTTC 3 cut(s) 529, 631, 649
HpyCH4III ACNGT 1 cut(s) 225
HpyCH4V TGCA 1 cut(s) 728
HpyF10VI GCNNNNNNNGC 6 cut(s) 208, 282, 387, 396, 490, 725
HpyF3I CTNAG 3 cut(s) 156, 470, 557
Hsp92I GRCGYC 1 cut(s) 690
Hsp92II CATG 2 cut(s) 167, 697
HspAI GCGC 4 cut(s) 279, 322, 385, 440
KpnI GGTACC 1 cut(s) 430
KroI GCCGGC 1 cut(s) 281
KroNI GCCGGC 1 cut(s) 283
Kzo9I GATC 4 cut(s) 159, 174, 366, 580
LguI GCTCTTC 2 cut(s) 44, 125
LmnI GCTCC 4 cut(s) 140, 216, 517, 597
Lsp1109I GCAGC 3 cut(s) 41, 374, 553
MaeI CTAG 1 cut(s) 683
MalI GATC 4 cut(s) 161, 176, 368, 582
MboI GATC 4 cut(s) 159, 174, 366, 580
MboII GAAGA 7 cut(s) 18, 21, 28, 61, 142, 259, 535
MfeI CAATTG 1 cut(s) 146
MflI RGATCY 3 cut(s) 159, 174, 580
MhlI GDGCHC 3 cut(s) 137, 213, 401
MluCI AATT 9 cut(s) 67, 73, 85, 91, 97, 103, 109, 146, 511
MlyI GAGTC 5 cut(s) 13, 369, 370, 428, 671
MroNI GCCGGC 1 cut(s) 281
MspI CCGG 7 cut(s) 114, 282, 431, 457, 485, 570, 590
MspR9I CCNGG 3 cut(s) 59, 403, 431
MunI CAATTG 1 cut(s) 146
MvaI CCWGG 2 cut(s) 59, 403
MvnI CGCG 2 cut(s) 279, 395
MwoI GCNNNNNNNGC 6 cut(s) 208, 282, 387, 396, 490, 725
NaeI GCCGGC 1 cut(s) 283
NciI CCSGG 1 cut(s) 431
NcoI CCATGG 1 cut(s) 693
NdeII GATC 4 cut(s) 159, 174, 366, 580
NgoMIV GCCGGC 1 cut(s) 281
NlaIII CATG 2 cut(s) 167, 697
NlaIV GGNNCC 2 cut(s) 428, 582
PciSI GCTCTTC 2 cut(s) 44, 125
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 336
PdiI GCCGGC 1 cut(s) 283
PfeI GAWTC 2 cut(s) 62, 152
PflFI GACNNNGTC 1 cut(s) 359
PkrI GCNGC 6 cut(s) 56, 278, 352, 389, 502, 568
PleI GAGTC 5 cut(s) 13, 368, 370, 427, 670
PpsI GAGTC 5 cut(s) 13, 368, 370, 427, 670
Psp124BI GAGCTC 2 cut(s) 137, 213
Psp6I CCWGG 2 cut(s) 57, 401
PspGI CCWGG 2 cut(s) 57, 401
PspN4I GGNNCC 2 cut(s) 428, 582
PspPI GGNCC 1 cut(s) 672
PsuI RGATCY 3 cut(s) 159, 174, 580
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 2 cut(s) 416, 428
RsaNI GTAC 2 cut(s) 415, 427
SacI GAGCTC 2 cut(s) 137, 213
SalI GTCGAC 1 cut(s) 299
SapI GCTCTTC 2 cut(s) 44, 125
SatI GCNGC 6 cut(s) 55, 277, 351, 388, 501, 567
Sau3AI GATC 4 cut(s) 159, 174, 366, 580
Sau96I GGNCC 1 cut(s) 672
SchI GAGTC 5 cut(s) 13, 369, 370, 428, 671
ScrFI CCNGG 3 cut(s) 59, 403, 431
SduI GDGCHC 3 cut(s) 137, 213, 401
Sse9I AATT 9 cut(s) 67, 73, 85, 91, 97, 103, 109, 146, 511
SsiI CCGC 6 cut(s) 277, 312, 348, 351, 501, 652
SspMI CTAG 1 cut(s) 683
SstI GAGCTC 2 cut(s) 137, 213
StyD4I CCNGG 3 cut(s) 57, 401, 429
StyI CCWWGG 1 cut(s) 693
TaaI ACNGT 1 cut(s) 225
TaqI TCGA 6 cut(s) 128, 252, 300, 339, 374, 449
TaqII GACCGA 1 cut(s) 372
TasI AATT 9 cut(s) 67, 73, 85, 91, 97, 103, 109, 146, 511
TauI GCSGC 3 cut(s) 279, 353, 503
TfiI GAWTC 2 cut(s) 62, 152
TseI GCWGC 3 cut(s) 54, 387, 566
TspGWI ACGGA 1 cut(s) 573
Tth111I GACNNNGTC 1 cut(s) 359
XapI RAATTY 1 cut(s) 85
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 300
XspI CTAG 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.