Rh4AG227300
ERF Family

DNA RNA polymerases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0006381
Physical Location & Seq
Forward (+)
52829380 .. 52831086
1707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG227300.1

Sequence Viewer

Length: 432 bp
ATGAGCTTCAGATCTCCACACTCTCCATCTCCACCAAGCAAACCGAGCTCCAGACCTCAGTCTCATCTTCACAACCTCATTGCCTTCGAGAGCCTCCTCATCTTCCACGGCCGAGCTGGCAACGAGAACGTCATCGACTGGCTCACCGCCATCGAGCGCCACTTCGCCTACCTCGACATCCCCTTCGCCGACCGAGTCAAGATCGCCCCCACTCGCTTGCGCGGCGACGCGAGCCCCTGGATGTGCTTGTACGAGTCTCGGTATCCGGCGAGTGCGCCATGGTCGATTTTCAGAAAAAGAGAGTCAGTCCGGGCTGTATATACCCTAGAACTACGAAAGCCAAATTTTCTGCACTCAGAAAGCTTCCGAAGCTCAACGTTCACAGAGACTCAGACCCCCATAATCCCCTACAGCGTCGCCACCAATCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag

Protein Analysis

143

Amino Acids

16.4

Weight (kDa)

9.72

Isoelectric Point (pI)

66.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 222, 230
AciI CCGC 2 cut(s) 147, 222
AclI AACGTT 1 cut(s) 377
AcoI YGGCCR 1 cut(s) 109
AcsI RAATTY 1 cut(s) 343
AfaI GTAC 1 cut(s) 251
AjnI CCWGG 1 cut(s) 236
AluBI AGCT 5 cut(s) 6, 48, 116, 363, 372
AluI AGCT 5 cut(s) 6, 48, 116, 363, 372
Alw21I GWGCWC 1 cut(s) 50
Alw26I GTCTC 3 cut(s) 66, 261, 380
AoxI GGCC 1 cut(s) 109
ApoI RAATTY 1 cut(s) 343
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AspLEI GCGC 3 cut(s) 159, 222, 277
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 1 cut(s) 136
BanII GRGCYC 2 cut(s) 50, 236
Bbv12I GWGCWC 1 cut(s) 50
BccI CCATC 2 cut(s) 34, 158
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 1 cut(s) 238
BciVI GTATCC 1 cut(s) 273
BcnI CCSGG 1 cut(s) 311
BcoDI GTCTC 3 cut(s) 66, 261, 380
BfaI CTAG 1 cut(s) 326
BfmI CTRYAG 1 cut(s) 409
BfoI RGCGCY 1 cut(s) 160
BfuI GTATCC 1 cut(s) 273
BglI GCCNNNNNGGC 1 cut(s) 117
BglII AGATCT 1 cut(s) 11
BisI GCNGC 1 cut(s) 223
BlsI GCNGC 1 cut(s) 224
Bme1390I CCNGG 2 cut(s) 238, 311
BmrFI CCNGG 2 cut(s) 238, 311
BoxI GACNNNNGTC 1 cut(s) 58
BpmI CTGGAG 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 311
BsaJI CCNNGG 3 cut(s) 106, 236, 278
Bse1I ACTGG 1 cut(s) 143
Bse3DI GCAATG 1 cut(s) 78
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 3 cut(s) 106, 236, 278
BseGI GGATG 2 cut(s) 177, 246
BseMI GCAATG 1 cut(s) 78
BseMII CTCAG 3 cut(s) 71, 369, 404
BseNI ACTGG 1 cut(s) 143
BseRI GAGGAG 1 cut(s) 86
BseX3I CGGCCG 1 cut(s) 109
BsgI GTGCAG 1 cut(s) 335
Bsh1236I CGCG 2 cut(s) 222, 230
Bsh1285I CGRYCG 2 cut(s) 112, 193
BshFI GGCC 1 cut(s) 111
BsiEI CGRYCG 2 cut(s) 112, 193
BsiHKAI GWGCWC 1 cut(s) 50
BsiSI CCGG 2 cut(s) 266, 310
BsmAI GTCTC 3 cut(s) 66, 261, 380
BsnI GGCC 1 cut(s) 111
Bsp1286I GDGCHC 2 cut(s) 50, 236
Bsp143I GATC 2 cut(s) 11, 201
Bsp19I CCATGG 1 cut(s) 278
BspACI CCGC 2 cut(s) 147, 222
BspANI GGCC 1 cut(s) 111
BspCNI CTCAG 3 cut(s) 70, 368, 403
BspFNI CGCG 2 cut(s) 222, 230
BsrDI GCAATG 1 cut(s) 78
BsrI ACTGG 1 cut(s) 143
BssECI CCNNGG 3 cut(s) 106, 236, 278
BssMI GATC 2 cut(s) 11, 201
BssT1I CCWWGG 1 cut(s) 278
Bst2UI CCWGG 1 cut(s) 238
BstC8I GCNNGC 3 cut(s) 118, 218, 232
BstDEI CTNAG 3 cut(s) 57, 355, 390
BstDSI CCRYGG 2 cut(s) 106, 278
BstF5I GGATG 2 cut(s) 177, 246
BstFNI CGCG 2 cut(s) 222, 230
BstH2I RGCGCY 1 cut(s) 160
BstHHI GCGC 3 cut(s) 159, 222, 277
BstKTI GATC 2 cut(s) 14, 204
BstMAI GTCTC 3 cut(s) 66, 261, 380
BstMBI GATC 2 cut(s) 11, 201
BstMCI CGRYCG 2 cut(s) 112, 193
BstMWI GCNNNNNNNGC 5 cut(s) 45, 117, 222, 231, 369
BstNI CCWGG 1 cut(s) 238
BstPAI GACNNNNGTC 1 cut(s) 58
BstSCI CCNGG 2 cut(s) 236, 309
BstSFI CTRYAG 1 cut(s) 409
BstUI CGCG 2 cut(s) 222, 230
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
BstZI CGGCCG 1 cut(s) 109
BsuI GTATCC 1 cut(s) 273
BsuRI GGCC 1 cut(s) 111
BtgI CCRYGG 2 cut(s) 106, 278
BtsCI GGATG 2 cut(s) 177, 246
Cac8I GCNNGC 3 cut(s) 118, 218, 232
CfoI GCGC 3 cut(s) 159, 222, 277
CseI GACGC 2 cut(s) 236, 403
Csp6I GTAC 1 cut(s) 250
CviAII CATG 1 cut(s) 279
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 3 cut(s) 57, 355, 390
DpnI GATC 2 cut(s) 13, 203
DpnII GATC 2 cut(s) 11, 201
EaeI YGGCCR 1 cut(s) 109
EagI CGGCCG 1 cut(s) 109
Ecl136II GAGCTC 1 cut(s) 48
EclXI CGGCCG 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 278
Eco24I GRGCYC 2 cut(s) 50, 236
Eco52I CGGCCG 1 cut(s) 109
Eco53kI GAGCTC 1 cut(s) 48
EcoICRI GAGCTC 1 cut(s) 48
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 278
EcoT38I GRGCYC 2 cut(s) 50, 236
ErhI CCWWGG 1 cut(s) 278
FaeI CATG 1 cut(s) 282
FaiI YATR 4 cut(s) 280, 319, 321, 401
FatI CATG 1 cut(s) 278
Fnu4HI GCNGC 1 cut(s) 223
FokI GGATG 2 cut(s) 164, 253
FriOI GRGCYC 2 cut(s) 50, 236
Fsp4HI GCNGC 1 cut(s) 223
FspBI CTAG 1 cut(s) 326
GlaI GCGC 3 cut(s) 158, 221, 276
GluI GCNGC 1 cut(s) 223
GsuI CTGGAG 1 cut(s) 34
HaeII RGCGCY 1 cut(s) 160
HaeIII GGCC 1 cut(s) 111
HapII CCGG 2 cut(s) 266, 310
HgaI GACGC 2 cut(s) 236, 403
HhaI GCGC 3 cut(s) 159, 222, 277
Hin1II CATG 1 cut(s) 282
Hin6I GCGC 3 cut(s) 157, 220, 275
HinP1I GCGC 3 cut(s) 157, 220, 275
HindIII AAGCTT 1 cut(s) 361
HinfI GANTC 4 cut(s) 195, 254, 302, 388
HpaII CCGG 2 cut(s) 266, 310
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 381
Hpy188I TCNGA 5 cut(s) 11, 293, 358, 368, 393
Hpy188III TCNNGA 3 cut(s) 51, 88, 199
Hpy8I GTNNAC 1 cut(s) 381
Hpy99I CGWCG 2 cut(s) 230, 419
HpyAV CCTTC 2 cut(s) 94, 193
HpyCH4IV ACGT 2 cut(s) 129, 377
HpyCH4V TGCA 1 cut(s) 352
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 117, 222, 231, 369
HpyF3I CTNAG 3 cut(s) 57, 355, 390
HpySE526I ACGT 2 cut(s) 129, 377
Hsp92II CATG 1 cut(s) 282
HspAI GCGC 3 cut(s) 157, 220, 275
Kzo9I GATC 2 cut(s) 11, 201
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 7 cut(s) 64, 102, 124, 223, 250, 279, 323
MaeI CTAG 1 cut(s) 326
MaeII ACGT 2 cut(s) 129, 377
MalI GATC 2 cut(s) 13, 203
MboI GATC 2 cut(s) 11, 201
MboII GAAGA 2 cut(s) 59, 94
MflI RGATCY 1 cut(s) 11
MhlI GDGCHC 2 cut(s) 50, 236
MluCI AATT 1 cut(s) 343
MlyI GAGTC 4 cut(s) 204, 263, 311, 382
MnlI CCTC 5 cut(s) 66, 86, 104, 107, 182
MspI CCGG 2 cut(s) 266, 310
MspR9I CCNGG 2 cut(s) 238, 311
MvaI CCWGG 1 cut(s) 238
MvnI CGCG 2 cut(s) 222, 230
MwoI GCNNNNNNNGC 5 cut(s) 45, 117, 222, 231, 369
NciI CCSGG 1 cut(s) 311
NcoI CCATGG 1 cut(s) 278
NdeII GATC 2 cut(s) 11, 201
NlaIII CATG 1 cut(s) 282
NmeAIII GCCGAG 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 1 cut(s) 224
PleI GAGTC 4 cut(s) 203, 262, 310, 382
PpsI GAGTC 4 cut(s) 203, 262, 310, 382
PshAI GACNNNNGTC 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 50
Psp1406I AACGTT 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PsuI RGATCY 1 cut(s) 11
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SacI GAGCTC 1 cut(s) 50
SatI GCNGC 1 cut(s) 223
Sau3AI GATC 2 cut(s) 11, 201
SchI GAGTC 4 cut(s) 204, 263, 311, 382
ScrFI CCNGG 2 cut(s) 238, 311
SduI GDGCHC 2 cut(s) 50, 236
SfcI CTRYAG 1 cut(s) 409
Sse9I AATT 1 cut(s) 343
SsiI CCGC 2 cut(s) 147, 222
SspMI CTAG 1 cut(s) 326
SstI GAGCTC 1 cut(s) 50
StyD4I CCNGG 2 cut(s) 236, 309
StyI CCWWGG 1 cut(s) 278
TaiI ACGT 2 cut(s) 132, 380
TaqI TCGA 5 cut(s) 87, 135, 153, 174, 284
TaqII GACCGA 1 cut(s) 207
TasI AATT 1 cut(s) 343
TauI GCSGC 1 cut(s) 225
TspGWI ACGGA 1 cut(s) 417
Tth111I GACNNNGTC 1 cut(s) 194
XapI RAATTY 1 cut(s) 343
XcmI CCANNNNNNNNNTGG 1 cut(s) 113
XspI CTAG 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.