Rh2AG088700

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006381
Physical Location & Seq
Forward (+)
7432005 .. 7434831
2827 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG088700.1

Sequence Viewer

Length: 720 bp
ATGGGAAGAAGAAAGAAGACTCCCTCCATTTCCACCATACCAGAAGTGAAGAGCTGCCTGGATTCCGATTCCAATTCCATTTCAAATTCCAATTCGAATTCCGGTGAAGCAAAATCGAAGAGCTCCATAGAGCAATTGGAATCTCAGATCCATGAGCTTCAGATCTCCACACTCTCCATCTCCACCAAGCAAACCGAGCTCCAGACCTCACAGTCTCACCTCCACAAGCTCATCGCCCTCGACAACCTCCCCGTCTTCCACGGCCGCGCCGGCAACGAGAACGCCGTCGACTGGATCACCGCCACCGAGCGCCACTTCGCCTACCTCGACATCCCCGCCGCCGACCGAGTCAAGATCGCCGCGACTCGCTTGCGCTGCGACGCGAGCCCCTGGATGTGCTGGTACGAGTCTCGGTACCCGGCGAGTGCGCCGTGGTCGATTTTCCGGCACGAGTTTCTGAGGTTTTTCCGGCCGGAGACGCTCATCGCCGCCGTGTGTAATTTGGAGCAGAAGAGGTCGGTGAAGGAGTATGAGGTGGAGTTTCTGAGGCTGGCTGCCGGAGGTGGGGATCCGTCGCCGGAGTGGAGCGATGAGGAGTTGACGAGGCATTTTGTTGGTGGATTGAAGGAGGAGATACGGCGGAAGGTGGAGTCGTTTAGGCCCAAAACCCTAGCTGACGCCATGGATTTTGCTCGCAATGTGGAGGCTGAAAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

239

Amino Acids

27.0

Weight (kDa)

6.19

Isoelectric Point (pI)

59.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 87 - 233 1.2e-08 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 118 - 210 1.4e-07 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 211
Acc65I GGTACC 1 cut(s) 414
AccB1I GGYRCC 1 cut(s) 414
AccI GTMKAC 1 cut(s) 288
AccII CGCG 3 cut(s) 267, 362, 383
AciI CCGC 7 cut(s) 265, 300, 336, 339, 360, 489, 640
AclWI GGATC 4 cut(s) 142, 302, 563, 576
AcoI YGGCCR 2 cut(s) 262, 470
AcsI RAATTY 2 cut(s) 85, 97
AcuI CTGAAG 1 cut(s) 143
AcyI GRCGYC 1 cut(s) 678
AfaI GTAC 2 cut(s) 404, 416
AfiI CCNNNNNNNGG 2 cut(s) 291, 564
AgsI TTSAA 2 cut(s) 84, 625
AjnI CCWGG 2 cut(s) 57, 389
AluBI AGCT 6 cut(s) 54, 123, 157, 199, 229, 674
AluI AGCT 6 cut(s) 54, 123, 157, 199, 229, 674
Alw21I GWGCWC 2 cut(s) 125, 201
Alw26I GTCTC 3 cut(s) 219, 414, 470
AlwI GGATC 4 cut(s) 142, 302, 563, 576
AoxI GGCC 3 cut(s) 262, 470, 659
ApeKI GCWGC 3 cut(s) 54, 375, 554
ApoI RAATTY 2 cut(s) 85, 97
Asp718I GGTACC 1 cut(s) 414
AspLEI GCGC 4 cut(s) 269, 312, 375, 430
AspS9I GGNCC 1 cut(s) 660
AsuC2I CCSGG 1 cut(s) 419
AsuHPI GGTGA 4 cut(s) 116, 209, 289, 532
AsuII TTCGAA 1 cut(s) 95
BamHI GGATCC 1 cut(s) 568
BanI GGYRCC 1 cut(s) 414
BanII GRGCYC 3 cut(s) 125, 201, 389
BauI CACGAG 1 cut(s) 449
BbsI GAAGAC 2 cut(s) 23, 247
Bbv12I GWGCWC 2 cut(s) 125, 201
BbvI GCAGC 3 cut(s) 41, 362, 541
BccI CCATC 1 cut(s) 185
BceAI ACGGC 5 cut(s) 269, 277, 415, 476, 653
BcgI CGANNNNNNTGC 2 cut(s) 352, 386
BciT130I CCWGG 2 cut(s) 59, 391
BcnI CCSGG 1 cut(s) 419
BcoDI GTCTC 3 cut(s) 219, 414, 470
BfaI CTAG 1 cut(s) 671
BfoI RGCGCY 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 270
BglII AGATCT 1 cut(s) 162
BisI GCNGC 7 cut(s) 55, 265, 339, 360, 376, 489, 555
BlsI GCNGC 7 cut(s) 56, 266, 340, 361, 377, 490, 556
Bme1390I CCNGG 3 cut(s) 59, 391, 419
BmgT120I GGNCC 1 cut(s) 660
BmiI GGNNCC 2 cut(s) 416, 570
BmrFI CCNGG 3 cut(s) 59, 391, 419
BpiI GAAGAC 2 cut(s) 23, 247
BpmI CTGGAG 1 cut(s) 185
Bpu14I TTCGAA 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 419
BsaHI GRCGYC 1 cut(s) 678
BsaJI CCNNGG 4 cut(s) 259, 389, 431, 681
BsaWI WCCGGW 1 cut(s) 101
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 564
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 1 cut(s) 703
BseBI CCWGG 2 cut(s) 59, 391
BseDI CCNNGG 4 cut(s) 259, 389, 431, 681
BseGI GGATG 2 cut(s) 330, 399
BseLI CCNNNNNNNGG 2 cut(s) 291, 564
BseMI GCAATG 1 cut(s) 703
BseMII CTCAG 3 cut(s) 158, 449, 536
BseNI ACTGG 1 cut(s) 296
BseRI GAGGAG 2 cut(s) 608, 644
BseX3I CGGCCG 2 cut(s) 262, 470
BseXI GCAGC 3 cut(s) 41, 362, 541
Bsh1236I CGCG 3 cut(s) 267, 362, 383
Bsh1285I CGRYCG 3 cut(s) 265, 346, 473
BshFI GGCC 3 cut(s) 264, 472, 661
BshNI GGYRCC 1 cut(s) 414
BsiEI CGRYCG 3 cut(s) 265, 346, 473
BsiHKAI GWGCWC 2 cut(s) 125, 201
BsiSI CCGG 8 cut(s) 102, 270, 419, 445, 469, 473, 558, 578
BslI CCNNNNNNNGG 2 cut(s) 291, 564
BsmAI GTCTC 3 cut(s) 219, 414, 470
BsmBI CGTCTC 1 cut(s) 470
BsnI GGCC 3 cut(s) 264, 472, 661
Bsp119I TTCGAA 1 cut(s) 95
Bsp1286I GDGCHC 3 cut(s) 125, 201, 389
Bsp143I GATC 5 cut(s) 147, 162, 294, 354, 568
Bsp19I CCATGG 1 cut(s) 681
BspACI CCGC 7 cut(s) 265, 300, 336, 339, 360, 489, 640
BspANI GGCC 3 cut(s) 264, 472, 661
BspCNI CTCAG 3 cut(s) 157, 450, 537
BspFNI CGCG 3 cut(s) 267, 362, 383
BspLI GGNNCC 2 cut(s) 416, 570
BspPI GGATC 4 cut(s) 142, 302, 563, 576
BspQI GCTCTTC 2 cut(s) 44, 113
BspT104I TTCGAA 1 cut(s) 95
BspT107I GGYRCC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 703
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 1 cut(s) 296
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 4 cut(s) 259, 389, 431, 681
BssMI GATC 5 cut(s) 147, 162, 294, 354, 568
BssNI GRCGYC 1 cut(s) 678
BssSI CACGAG 1 cut(s) 449
BssT1I CCWWGG 1 cut(s) 681
Bst2BI CACGAG 1 cut(s) 449
Bst2UI CCWGG 2 cut(s) 59, 391
Bst4CI ACNGT 1 cut(s) 213
Bst6I CTCTTC 3 cut(s) 44, 113, 506
BstACI GRCGYC 1 cut(s) 678
BstBI TTCGAA 1 cut(s) 95
BstC8I GCNNGC 5 cut(s) 271, 371, 385, 552, 694
BstDEI CTNAG 3 cut(s) 144, 458, 545
BstDSI CCRYGG 3 cut(s) 259, 431, 681
BstF5I GGATG 2 cut(s) 330, 399
BstFNI CGCG 3 cut(s) 267, 362, 383
BstH2I RGCGCY 1 cut(s) 313
BstHHI GCGC 4 cut(s) 269, 312, 375, 430
BstKTI GATC 5 cut(s) 150, 165, 297, 357, 571
BstMAI GTCTC 3 cut(s) 219, 414, 470
BstMBI GATC 5 cut(s) 147, 162, 294, 354, 568
BstMCI CGRYCG 3 cut(s) 265, 346, 473
BstMWI GCNNNNNNNGC 6 cut(s) 196, 270, 375, 384, 478, 713
BstNI CCWGG 2 cut(s) 59, 391
BstSCI CCNGG 3 cut(s) 57, 389, 417
BstUI CGCG 3 cut(s) 267, 362, 383
BstV1I GCAGC 3 cut(s) 41, 362, 541
BstV2I GAAGAC 2 cut(s) 23, 247
BstX2I RGATCY 3 cut(s) 147, 162, 568
BstYI RGATCY 3 cut(s) 147, 162, 568
BstZI CGGCCG 2 cut(s) 262, 470
BsuRI GGCC 3 cut(s) 264, 472, 661
BtgI CCRYGG 3 cut(s) 259, 431, 681
BtgZI GCGATG 3 cut(s) 217, 469, 603
BtsCI GGATG 2 cut(s) 330, 399
Cac8I GCNNGC 5 cut(s) 271, 371, 385, 552, 694
CfoI GCGC 4 cut(s) 269, 312, 375, 430
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 1 cut(s) 660
CseI GACGC 3 cut(s) 389, 487, 686
Csp6I GTAC 2 cut(s) 403, 415
CviAII CATG 2 cut(s) 152, 682
CviQI GTAC 2 cut(s) 403, 415
DdeI CTNAG 3 cut(s) 144, 458, 545
DpnI GATC 5 cut(s) 149, 164, 296, 356, 570
DpnII GATC 5 cut(s) 147, 162, 294, 354, 568
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
EaeI YGGCCR 2 cut(s) 262, 470
EagI CGGCCG 2 cut(s) 262, 470
Eam1104I CTCTTC 3 cut(s) 44, 113, 506
EarI CTCTTC 3 cut(s) 44, 113, 506
EciI GGCGGA 1 cut(s) 655
Ecl136II GAGCTC 2 cut(s) 123, 199
EclXI CGGCCG 2 cut(s) 262, 470
Eco130I CCWWGG 1 cut(s) 681
Eco24I GRGCYC 3 cut(s) 125, 201, 389
Eco52I CGGCCG 2 cut(s) 262, 470
Eco53kI GAGCTC 2 cut(s) 123, 199
Eco57I CTGAAG 1 cut(s) 143
EcoICRI GAGCTC 2 cut(s) 123, 199
EcoRI GAATTC 1 cut(s) 97
EcoRII CCWGG 2 cut(s) 57, 389
EcoT14I CCWWGG 1 cut(s) 681
EcoT38I GRGCYC 3 cut(s) 125, 201, 389
ErhI CCWWGG 1 cut(s) 681
Esp3I CGTCTC 1 cut(s) 470
FaeI CATG 2 cut(s) 155, 685
FaiI YATR 6 cut(s) 38, 128, 153, 531, 683, 718
FatI CATG 2 cut(s) 151, 681
FauI CCCGC 1 cut(s) 343
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 7 cut(s) 55, 265, 339, 360, 376, 489, 555
FokI GGATG 2 cut(s) 317, 406
FriOI GRGCYC 3 cut(s) 125, 201, 389
Fsp4HI GCNGC 7 cut(s) 55, 265, 339, 360, 376, 489, 555
FspBI CTAG 1 cut(s) 671
GlaI GCGC 4 cut(s) 268, 311, 374, 429
GluI GCNGC 7 cut(s) 55, 265, 339, 360, 376, 489, 555
GsuI CTGGAG 1 cut(s) 185
HaeII RGCGCY 1 cut(s) 313
HaeIII GGCC 3 cut(s) 264, 472, 661
HapII CCGG 8 cut(s) 102, 270, 419, 445, 469, 473, 558, 578
HgaI GACGC 3 cut(s) 389, 487, 686
HhaI GCGC 4 cut(s) 269, 312, 375, 430
Hin1I GRCGYC 1 cut(s) 678
Hin1II CATG 2 cut(s) 155, 685
Hin6I GCGC 4 cut(s) 267, 310, 373, 428
HinP1I GCGC 4 cut(s) 267, 310, 373, 428
HincII GTYRAC 2 cut(s) 289, 600
HindII GTYRAC 2 cut(s) 289, 600
HinfI GANTC 8 cut(s) 19, 62, 68, 140, 348, 364, 407, 650
HpaII CCGG 8 cut(s) 102, 270, 419, 445, 469, 473, 558, 578
HphI GGTGA 4 cut(s) 116, 209, 289, 532
Hpy166II GTNNAC 2 cut(s) 289, 600
Hpy188I TCNGA 5 cut(s) 67, 147, 162, 459, 546
Hpy188III TCNNGA 2 cut(s) 202, 352
Hpy8I GTNNAC 2 cut(s) 289, 600
Hpy99I CGWCG 3 cut(s) 290, 383, 577
HpyAV CCTTC 3 cut(s) 517, 619, 637
HpyCH4III ACNGT 1 cut(s) 213
HpyCH4V TGCA 1 cut(s) 716
HpyF10VI GCNNNNNNNGC 6 cut(s) 196, 270, 375, 384, 478, 713
HpyF3I CTNAG 3 cut(s) 144, 458, 545
Hsp92I GRCGYC 1 cut(s) 678
Hsp92II CATG 2 cut(s) 155, 685
HspAI GCGC 4 cut(s) 267, 310, 373, 428
KpnI GGTACC 1 cut(s) 418
KroI GCCGGC 1 cut(s) 269
KroNI GCCGGC 1 cut(s) 271
Kzo9I GATC 5 cut(s) 147, 162, 294, 354, 568
LguI GCTCTTC 2 cut(s) 44, 113
LmnI GCTCC 4 cut(s) 128, 204, 505, 585
Lsp1109I GCAGC 3 cut(s) 41, 362, 541
MaeI CTAG 1 cut(s) 671
MalI GATC 5 cut(s) 149, 164, 296, 356, 570
MboI GATC 5 cut(s) 147, 162, 294, 354, 568
MboII GAAGA 7 cut(s) 18, 21, 28, 61, 130, 247, 523
MfeI CAATTG 1 cut(s) 134
MflI RGATCY 3 cut(s) 147, 162, 568
MhlI GDGCHC 3 cut(s) 125, 201, 389
MluCI AATT 6 cut(s) 73, 85, 91, 97, 134, 499
MlyI GAGTC 5 cut(s) 13, 357, 358, 416, 659
MroNI GCCGGC 1 cut(s) 269
MspI CCGG 8 cut(s) 102, 270, 419, 445, 469, 473, 558, 578
MspR9I CCNGG 3 cut(s) 59, 391, 419
MunI CAATTG 1 cut(s) 134
MvaI CCWGG 2 cut(s) 59, 391
MvnI CGCG 3 cut(s) 267, 362, 383
MwoI GCNNNNNNNGC 6 cut(s) 196, 270, 375, 384, 478, 713
NaeI GCCGGC 1 cut(s) 271
NciI CCSGG 1 cut(s) 419
NcoI CCATGG 1 cut(s) 681
NdeII GATC 5 cut(s) 147, 162, 294, 354, 568
NgoMIV GCCGGC 1 cut(s) 269
NlaIII CATG 2 cut(s) 155, 685
NlaIV GGNNCC 2 cut(s) 416, 570
NspV TTCGAA 1 cut(s) 95
PciSI GCTCTTC 2 cut(s) 44, 113
PcsI WCGNNNNNNNCGW 2 cut(s) 282, 324
PdiI GCCGGC 1 cut(s) 271
PfeI GAWTC 3 cut(s) 62, 68, 140
PflFI GACNNNGTC 1 cut(s) 347
PkrI GCNGC 7 cut(s) 56, 266, 340, 361, 377, 490, 556
PleI GAGTC 5 cut(s) 13, 356, 358, 415, 658
PpsI GAGTC 5 cut(s) 13, 356, 358, 415, 658
Psp124BI GAGCTC 2 cut(s) 125, 201
Psp6I CCWGG 2 cut(s) 57, 389
PspGI CCWGG 2 cut(s) 57, 389
PspN4I GGNNCC 2 cut(s) 416, 570
PspPI GGNCC 1 cut(s) 660
PsuI RGATCY 3 cut(s) 147, 162, 568
PsyI GACNNNGTC 1 cut(s) 347
RsaI GTAC 2 cut(s) 404, 416
RsaNI GTAC 2 cut(s) 403, 415
SacI GAGCTC 2 cut(s) 125, 201
SalI GTCGAC 1 cut(s) 287
SapI GCTCTTC 2 cut(s) 44, 113
SatI GCNGC 7 cut(s) 55, 265, 339, 360, 376, 489, 555
Sau3AI GATC 5 cut(s) 147, 162, 294, 354, 568
Sau96I GGNCC 1 cut(s) 660
SchI GAGTC 5 cut(s) 13, 357, 358, 416, 659
ScrFI CCNGG 3 cut(s) 59, 391, 419
SduI GDGCHC 3 cut(s) 125, 201, 389
SfuI TTCGAA 1 cut(s) 95
Sse9I AATT 6 cut(s) 73, 85, 91, 97, 134, 499
SsiI CCGC 7 cut(s) 265, 300, 336, 339, 360, 489, 640
SspMI CTAG 1 cut(s) 671
SstI GAGCTC 2 cut(s) 125, 201
StyD4I CCNGG 3 cut(s) 57, 389, 417
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 1 cut(s) 213
TaqI TCGA 6 cut(s) 95, 116, 240, 288, 327, 437
TaqII GACCGA 1 cut(s) 360
TasI AATT 6 cut(s) 73, 85, 91, 97, 134, 499
TauI GCSGC 4 cut(s) 267, 341, 362, 491
TfiI GAWTC 3 cut(s) 62, 68, 140
TseI GCWGC 3 cut(s) 54, 375, 554
TspGWI ACGGA 1 cut(s) 561
Tth111I GACNNNGTC 1 cut(s) 347
XapI RAATTY 2 cut(s) 85, 97
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.