Rroxscaffold_3G00257090

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006381
Physical Location & Seq
Forward (+)
51510127 .. 51510498
372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00257090.1

Sequence Viewer

Length: 372 bp
ATGAGCTTCATATCTCCACACTCTCCATCTCCACCAAGCAAACCGAGCTCCGGACCTCGGTCTCATCTCCACAACCTCATTGCCTTCGAGAACCTCCTCATCTTCCATGGCCGCGGCAACGAGAACGTCGTCGGCCGGCTCACCGCCACCGAGCGCCACTTCGCCTACCTTGACATCCCCTTCGCCGTCCTAGTCAAGATCGCCCCCACTCGCTTGAGCGGCAACGCGAGCCCTTGGATGTGTTGGTACGAGTCTCGGTATCCGGCGAGTACGCCATGGTCGATTTTCGGTATGAGTTTGCGGTTTTCGAGCCGGAGACGCTCATCGTCGCCGTGTGTAATTTGGAGCGAAGAGGTCGGTGAAGGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

123

Amino Acids

13.75

Weight (kDa)

9.58

Isoelectric Point (pI)

77.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 219
AccII CGCG 2 cut(s) 114, 227
AccIII TCCGGA 1 cut(s) 50
AciI CCGC 5 cut(s) 112, 114, 144, 219, 301
AcoI YGGCCR 2 cut(s) 109, 133
AfaI GTAC 2 cut(s) 248, 271
AfiI CCNNNNNNNGG 2 cut(s) 50, 57
AluBI AGCT 2 cut(s) 6, 48
AluI AGCT 2 cut(s) 6, 48
Alw21I GWGCWC 1 cut(s) 50
Alw26I GTCTC 3 cut(s) 66, 258, 310
Aor13HI TCCGGA 1 cut(s) 50
AoxI GGCC 2 cut(s) 109, 133
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 53
AsuHPI GGTGA 2 cut(s) 133, 371
AvaII GGWCC 1 cut(s) 53
BanII GRGCYC 2 cut(s) 50, 233
Bbv12I GWGCWC 1 cut(s) 50
BccI CCATC 1 cut(s) 34
BceAI ACGGC 2 cut(s) 170, 316
BciVI GTATCC 1 cut(s) 270
BcoDI GTCTC 3 cut(s) 66, 258, 310
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 1 cut(s) 157
BfuI GTATCC 1 cut(s) 270
BisI GCNGC 3 cut(s) 112, 115, 220
BlsI GCNGC 3 cut(s) 113, 116, 221
Bme18I GGWCC 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 53
BoxI GACNNNNGTC 1 cut(s) 58
BpuEI CTTGAG 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 66
BsaJI CCNNGG 5 cut(s) 56, 106, 112, 233, 275
BsaWI WCCGGW 1 cut(s) 50
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 57
Bse118I RCCGGY 1 cut(s) 135
Bse3DI GCAATG 1 cut(s) 78
BseAI TCCGGA 1 cut(s) 50
BseDI CCNNGG 5 cut(s) 56, 106, 112, 233, 275
BseGI GGATG 2 cut(s) 174, 243
BseLI CCNNNNNNNGG 2 cut(s) 50, 57
BseMI GCAATG 1 cut(s) 78
BseRI GAGGAG 1 cut(s) 86
BseX3I CGGCCG 1 cut(s) 133
Bsh1236I CGCG 2 cut(s) 114, 227
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 2 cut(s) 111, 135
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 50
BsiSI CCGG 4 cut(s) 51, 136, 263, 313
BslI CCNNNNNNNGG 2 cut(s) 50, 57
BsmAI GTCTC 3 cut(s) 66, 258, 310
BsmBI CGTCTC 1 cut(s) 310
BsnI GGCC 2 cut(s) 111, 135
Bso31I GGTCTC 1 cut(s) 66
Bsp1286I GDGCHC 2 cut(s) 50, 233
Bsp13I TCCGGA 1 cut(s) 50
Bsp143I GATC 1 cut(s) 198
Bsp19I CCATGG 2 cut(s) 106, 275
BspACI CCGC 5 cut(s) 112, 114, 144, 219, 301
BspANI GGCC 2 cut(s) 111, 135
BspEI TCCGGA 1 cut(s) 50
BspFNI CGCG 2 cut(s) 114, 227
BspTNI GGTCTC 1 cut(s) 66
BsrBI CCGCTC 1 cut(s) 219
BsrDI GCAATG 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 135
BssAI RCCGGY 1 cut(s) 135
BssECI CCNNGG 5 cut(s) 56, 106, 112, 233, 275
BssMI GATC 1 cut(s) 198
BssT1I CCWWGG 3 cut(s) 106, 233, 275
Bst6I CTCTTC 1 cut(s) 345
BstC8I GCNNGC 2 cut(s) 137, 229
BstDSI CCRYGG 3 cut(s) 106, 112, 275
BstF5I GGATG 2 cut(s) 174, 243
BstFNI CGCG 2 cut(s) 114, 227
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 1 cut(s) 201
BstMAI GTCTC 3 cut(s) 66, 258, 310
BstMBI GATC 1 cut(s) 198
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 4 cut(s) 45, 219, 228, 318
BstPAI GACNNNNGTC 1 cut(s) 58
BstUI CGCG 2 cut(s) 114, 227
BstZI CGGCCG 1 cut(s) 133
BsuI GTATCC 1 cut(s) 270
BsuRI GGCC 2 cut(s) 111, 135
BtgI CCRYGG 3 cut(s) 106, 112, 275
BtsCI GGATG 2 cut(s) 174, 243
Cac8I GCNNGC 2 cut(s) 137, 229
CfoI GCGC 1 cut(s) 156
Cfr10I RCCGGY 1 cut(s) 135
Cfr13I GGNCC 1 cut(s) 53
Cfr42I CCGCGG 1 cut(s) 115
CseI GACGC 1 cut(s) 327
Csp6I GTAC 2 cut(s) 247, 270
CviAII CATG 2 cut(s) 107, 276
CviJI RGCY 7 cut(s) 6, 48, 111, 135, 139, 231, 312
CviKI_1 RGCY 7 cut(s) 6, 48, 111, 135, 139, 231, 312
CviQI GTAC 2 cut(s) 247, 270
DpnI GATC 1 cut(s) 200
DpnII GATC 1 cut(s) 198
EaeI YGGCCR 2 cut(s) 109, 133
EagI CGGCCG 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
Ecl136II GAGCTC 1 cut(s) 48
EclXI CGGCCG 1 cut(s) 133
Eco130I CCWWGG 3 cut(s) 106, 233, 275
Eco24I GRGCYC 2 cut(s) 50, 233
Eco31I GGTCTC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 53
Eco52I CGGCCG 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 48
EcoICRI GAGCTC 1 cut(s) 48
EcoT14I CCWWGG 3 cut(s) 106, 233, 275
EcoT38I GRGCYC 2 cut(s) 50, 233
ErhI CCWWGG 3 cut(s) 106, 233, 275
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 2 cut(s) 110, 279
FaiI YATR 5 cut(s) 11, 108, 277, 293, 370
FatI CATG 2 cut(s) 106, 275
Fnu4HI GCNGC 3 cut(s) 112, 115, 220
FokI GGATG 2 cut(s) 161, 250
FriOI GRGCYC 2 cut(s) 50, 233
Fsp4HI GCNGC 3 cut(s) 112, 115, 220
FspBI CTAG 1 cut(s) 191
GlaI GCGC 1 cut(s) 155
GluI GCNGC 3 cut(s) 112, 115, 220
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 2 cut(s) 111, 135
HapII CCGG 4 cut(s) 51, 136, 263, 313
HgaI GACGC 1 cut(s) 327
HhaI GCGC 1 cut(s) 156
Hin1II CATG 2 cut(s) 110, 279
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HinfI GANTC 1 cut(s) 251
HpaII CCGG 4 cut(s) 51, 136, 263, 313
HphI GGTGA 2 cut(s) 133, 371
Hpy188III TCNNGA 3 cut(s) 51, 88, 196
Hpy99I CGWCG 3 cut(s) 131, 134, 331
HpyAV CCTTC 3 cut(s) 94, 190, 356
HpyCH4IV ACGT 1 cut(s) 126
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 219, 228, 318
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 2 cut(s) 110, 279
HspAI GCGC 1 cut(s) 154
Kpn2I TCCGGA 1 cut(s) 50
KroI GCCGGC 1 cut(s) 135
KroNI GCCGGC 1 cut(s) 137
KspI CCGCGG 1 cut(s) 115
Kzo9I GATC 1 cut(s) 198
LmnI GCTCC 2 cut(s) 53, 345
LpnPI CCDG 4 cut(s) 64, 149, 276, 326
MaeI CTAG 1 cut(s) 191
MaeII ACGT 1 cut(s) 126
MalI GATC 1 cut(s) 200
MbiI CCGCTC 1 cut(s) 219
MboI GATC 1 cut(s) 198
MboII GAAGA 2 cut(s) 94, 362
MhlI GDGCHC 2 cut(s) 50, 233
MluCI AATT 1 cut(s) 339
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 5 cut(s) 66, 86, 104, 107, 346
MroI TCCGGA 1 cut(s) 50
MroNI GCCGGC 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 114
MspI CCGG 4 cut(s) 51, 136, 263, 313
MvnI CGCG 2 cut(s) 114, 227
MwoI GCNNNNNNNGC 4 cut(s) 45, 219, 228, 318
NaeI GCCGGC 1 cut(s) 137
NcoI CCATGG 2 cut(s) 106, 275
NdeII GATC 1 cut(s) 198
NgoMIV GCCGGC 1 cut(s) 135
NlaIII CATG 2 cut(s) 110, 279
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 278
PdiI GCCGGC 1 cut(s) 137
PkrI GCNGC 3 cut(s) 113, 116, 221
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PshAI GACNNNNGTC 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 50
PspPI GGNCC 1 cut(s) 53
RsaI GTAC 2 cut(s) 248, 271
RsaNI GTAC 2 cut(s) 247, 270
SacI GAGCTC 1 cut(s) 50
SacII CCGCGG 1 cut(s) 115
SatI GCNGC 3 cut(s) 112, 115, 220
Sau3AI GATC 1 cut(s) 198
Sau96I GGNCC 1 cut(s) 53
SchI GAGTC 1 cut(s) 260
SduI GDGCHC 2 cut(s) 50, 233
SetI ASST 9 cut(s) 8, 50, 58, 78, 96, 129, 171, 357, 367
Sfr303I CCGCGG 1 cut(s) 115
SgrBI CCGCGG 1 cut(s) 115
SinI GGWCC 1 cut(s) 53
SmlI CTYRAG 1 cut(s) 214
SmoI CTYRAG 1 cut(s) 214
Sse9I AATT 1 cut(s) 339
SsiI CCGC 5 cut(s) 112, 114, 144, 219, 301
SspMI CTAG 1 cut(s) 191
SstI GAGCTC 1 cut(s) 50
StyI CCWWGG 3 cut(s) 106, 233, 275
TaiI ACGT 1 cut(s) 129
TaqI TCGA 3 cut(s) 87, 281, 308
TaqII GACCGA 1 cut(s) 48
TasI AATT 1 cut(s) 339
TauI GCSGC 3 cut(s) 114, 117, 222
VpaK11BI GGWCC 1 cut(s) 53
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.