Rh2AG365300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006381
Physical Location & Seq
Forward (+)
54127173 .. 54127541
369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG365300.1

Sequence Viewer

Length: 369 bp
ATGAGCTTCATATCTCCACACTCTCCATCTCCACCAAGCAAACCAAGCTCCAGACCTCAGTCTCATCTTCACAACCTCATTGCCTTCAAGAACCTCCTCATCTTCCACGGCCGCGCTGGCAACGAGAACGTAGTCGACTGGCTCACCGCCACCGAGCGCCACTTCGCCTACCTCGACATCCCCTTCGCCGACCGAGTCAAGATCGCCCCCACTTGCTTGCGCGGCGACGCGAGCCCCTGGATGTGCTTGTACGAGTCTCGGTATCCGGCGAGTGCGCCATGGTCGATTTTCAGGTATGAGTTTTACAGTTTTTCCGCCCGGAGACGCTCATCGCCGCCGTGTGTAATTTGGAGCAGAAGAGGTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

122

Amino Acids

14.07

Weight (kDa)

10.03

Isoelectric Point (pI)

82.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AccII CGCG 3 cut(s) 114, 222, 230
AciI CCGC 5 cut(s) 112, 147, 222, 315, 335
AcoI YGGCCR 1 cut(s) 109
AfaI GTAC 1 cut(s) 251
AgsI TTSAA 1 cut(s) 88
AjnI CCWGG 1 cut(s) 236
AluBI AGCT 2 cut(s) 6, 48
AluI AGCT 2 cut(s) 6, 48
Alw26I GTCTC 3 cut(s) 66, 261, 316
AoxI GGCC 1 cut(s) 109
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AspLEI GCGC 4 cut(s) 116, 159, 222, 277
AsuC2I CCSGG 1 cut(s) 319
AsuHPI GGTGA 1 cut(s) 136
BanII GRGCYC 1 cut(s) 236
BccI CCATC 1 cut(s) 34
BceAI ACGGC 2 cut(s) 124, 322
BciT130I CCWGG 1 cut(s) 238
BciVI GTATCC 1 cut(s) 273
BcnI CCSGG 1 cut(s) 319
BcoDI GTCTC 3 cut(s) 66, 261, 316
BfoI RGCGCY 1 cut(s) 160
BfuI GTATCC 1 cut(s) 273
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 3 cut(s) 112, 223, 335
BlsI GCNGC 3 cut(s) 113, 224, 336
Bme1390I CCNGG 2 cut(s) 238, 319
BmrFI CCNGG 2 cut(s) 238, 319
BoxI GACNNNNGTC 1 cut(s) 58
BpmI CTGGAG 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 319
BsaJI CCNNGG 3 cut(s) 106, 236, 278
Bse1I ACTGG 1 cut(s) 143
Bse3DI GCAATG 1 cut(s) 78
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 3 cut(s) 106, 236, 278
BseGI GGATG 2 cut(s) 177, 246
BseMI GCAATG 1 cut(s) 78
BseMII CTCAG 1 cut(s) 71
BseNI ACTGG 1 cut(s) 143
BseRI GAGGAG 1 cut(s) 86
BseX3I CGGCCG 1 cut(s) 109
Bsh1236I CGCG 3 cut(s) 114, 222, 230
Bsh1285I CGRYCG 2 cut(s) 112, 193
BshFI GGCC 1 cut(s) 111
BsiEI CGRYCG 2 cut(s) 112, 193
BsiSI CCGG 2 cut(s) 266, 319
BsmAI GTCTC 3 cut(s) 66, 261, 316
BsmBI CGTCTC 1 cut(s) 316
BsnI GGCC 1 cut(s) 111
Bsp1286I GDGCHC 1 cut(s) 236
Bsp143I GATC 1 cut(s) 201
Bsp19I CCATGG 1 cut(s) 278
BspACI CCGC 5 cut(s) 112, 147, 222, 315, 335
BspANI GGCC 1 cut(s) 111
BspCNI CTCAG 1 cut(s) 70
BspFNI CGCG 3 cut(s) 114, 222, 230
BsrDI GCAATG 1 cut(s) 78
BsrI ACTGG 1 cut(s) 143
BssECI CCNNGG 3 cut(s) 106, 236, 278
BssMI GATC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 278
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 1 cut(s) 308
Bst6I CTCTTC 1 cut(s) 352
BstC8I GCNNGC 3 cut(s) 118, 218, 232
BstDEI CTNAG 1 cut(s) 57
BstDSI CCRYGG 2 cut(s) 106, 278
BstF5I GGATG 2 cut(s) 177, 246
BstFNI CGCG 3 cut(s) 114, 222, 230
BstH2I RGCGCY 1 cut(s) 160
BstHHI GCGC 4 cut(s) 116, 159, 222, 277
BstKTI GATC 1 cut(s) 204
BstMAI GTCTC 3 cut(s) 66, 261, 316
BstMBI GATC 1 cut(s) 201
BstMCI CGRYCG 2 cut(s) 112, 193
BstMWI GCNNNNNNNGC 4 cut(s) 45, 117, 222, 231
BstNI CCWGG 1 cut(s) 238
BstPAI GACNNNNGTC 1 cut(s) 58
BstSCI CCNGG 2 cut(s) 236, 317
BstUI CGCG 3 cut(s) 114, 222, 230
BstZI CGGCCG 1 cut(s) 109
BsuI GTATCC 1 cut(s) 273
BsuRI GGCC 1 cut(s) 111
BtgI CCRYGG 2 cut(s) 106, 278
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 2 cut(s) 177, 246
Cac8I GCNNGC 3 cut(s) 118, 218, 232
CfoI GCGC 4 cut(s) 116, 159, 222, 277
CseI GACGC 2 cut(s) 236, 333
Csp6I GTAC 1 cut(s) 250
CviAII CATG 1 cut(s) 279
CviJI RGCY 5 cut(s) 6, 48, 111, 142, 234
CviKI_1 RGCY 5 cut(s) 6, 48, 111, 142, 234
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 1 cut(s) 57
DpnI GATC 1 cut(s) 203
DpnII GATC 1 cut(s) 201
EaeI YGGCCR 1 cut(s) 109
EagI CGGCCG 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
EciI GGCGGA 1 cut(s) 304
EclXI CGGCCG 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 278
Eco24I GRGCYC 1 cut(s) 236
Eco52I CGGCCG 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 278
EcoT38I GRGCYC 1 cut(s) 236
ErhI CCWWGG 1 cut(s) 278
Esp3I CGTCTC 1 cut(s) 316
FaeI CATG 1 cut(s) 282
FaiI YATR 3 cut(s) 11, 280, 297
FatI CATG 1 cut(s) 278
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 3 cut(s) 112, 223, 335
FokI GGATG 2 cut(s) 164, 253
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 3 cut(s) 112, 223, 335
GlaI GCGC 4 cut(s) 115, 158, 221, 276
GluI GCNGC 3 cut(s) 112, 223, 335
GsuI CTGGAG 1 cut(s) 34
HaeII RGCGCY 1 cut(s) 160
HaeIII GGCC 1 cut(s) 111
HapII CCGG 2 cut(s) 266, 319
HgaI GACGC 2 cut(s) 236, 333
HhaI GCGC 4 cut(s) 116, 159, 222, 277
Hin1II CATG 1 cut(s) 282
Hin6I GCGC 4 cut(s) 114, 157, 220, 275
HinP1I GCGC 4 cut(s) 114, 157, 220, 275
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 2 cut(s) 195, 254
HpaII CCGG 2 cut(s) 266, 319
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 136
Hpy188III TCNNGA 3 cut(s) 51, 88, 199
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 2 cut(s) 94, 193
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4IV ACGT 1 cut(s) 129
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 117, 222, 231
HpyF3I CTNAG 1 cut(s) 57
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 1 cut(s) 282
HspAI GCGC 4 cut(s) 114, 157, 220, 275
Kzo9I GATC 1 cut(s) 201
LmnI GCTCC 2 cut(s) 53, 351
LpnPI CCDG 8 cut(s) 64, 102, 124, 223, 250, 277, 279, 332
MaeII ACGT 1 cut(s) 129
MalI GATC 1 cut(s) 203
MboI GATC 1 cut(s) 201
MboII GAAGA 3 cut(s) 59, 94, 369
MhlI GDGCHC 1 cut(s) 236
MluCI AATT 1 cut(s) 345
MlyI GAGTC 2 cut(s) 204, 263
MnlI CCTC 6 cut(s) 66, 86, 104, 107, 182, 353
MspI CCGG 2 cut(s) 266, 319
MspR9I CCNGG 2 cut(s) 238, 319
MvaI CCWGG 1 cut(s) 238
MvnI CGCG 3 cut(s) 114, 222, 230
MwoI GCNNNNNNNGC 4 cut(s) 45, 117, 222, 231
NciI CCSGG 1 cut(s) 319
NcoI CCATGG 1 cut(s) 278
NdeII GATC 1 cut(s) 201
NlaIII CATG 1 cut(s) 282
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 3 cut(s) 113, 224, 336
PleI GAGTC 2 cut(s) 203, 262
PpsI GAGTC 2 cut(s) 203, 262
PshAI GACNNNNGTC 1 cut(s) 58
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SalI GTCGAC 1 cut(s) 134
SatI GCNGC 3 cut(s) 112, 223, 335
Sau3AI GATC 1 cut(s) 201
SchI GAGTC 2 cut(s) 204, 263
ScrFI CCNGG 2 cut(s) 238, 319
SduI GDGCHC 1 cut(s) 236
SetI ASST 9 cut(s) 8, 50, 58, 78, 96, 132, 174, 296, 364
Sse9I AATT 1 cut(s) 345
SsiI CCGC 5 cut(s) 112, 147, 222, 315, 335
StyD4I CCNGG 2 cut(s) 236, 317
StyI CCWWGG 1 cut(s) 278
TaaI ACNGT 1 cut(s) 308
TaiI ACGT 1 cut(s) 132
TaqI TCGA 3 cut(s) 135, 174, 284
TaqII GACCGA 1 cut(s) 207
TasI AATT 1 cut(s) 345
TauI GCSGC 3 cut(s) 114, 225, 337
Tth111I GACNNNGTC 1 cut(s) 194
XcmI CCANNNNNNNNNTGG 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.