RchiOBHm_Chr2g0148731

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008898
Physical Location & Seq
Forward (+)
66501327 .. 66502925
1599 bp
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UTR
Exon/CDS
Intron
PRQ51826

Sequence Viewer

Length: 543 bp
ATGACGACTCGAATAGCTCTGGGAGTCGGAGCTAACTTGCTCGGTCAACACACCGCGAGAGAAACCAGGACGCCATTGCTGACGTCGGCAATCTCGACCCTCAGGGTTTTGGCATTTGAGTTCGTTAGTGAAGAATTGCTGTGGGAGCAATTTGTTCGAGCCGGTCCTATTGTTAATGTGTATGTTCCTAAAGATAGAGTTATGAACCTCCATCAAGGATATAGATTCGTGGAGTTCCGAAGTGAAGAGGATGTTGATTACATAAGTTTTCCTATTCTCAAGTTATTATATAGCTCCCCGATTCCTGTTAAAGATGTTGTTTCTGACTATAAATTTGTTGTTTTGACTGCCATGCCTACACTCAAGGTGCTTAATATGATCAACCTTTACGGAAAGGCAATTCGTATAATGAGAGAACCTGAAACTGGAAACTCCTGTGGATTTGGTTTCATTAGCTATGATTCCTTTGAGCCATCTGATGCAGCTATTGAGGTATGTCCTTCACTATCCTTTATTATACTTTCTATATGGTTATTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1

Protein Analysis

180

Amino Acids

20.32

Weight (kDa)

5.42

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 42 - 86 7.4e-06 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 86
AccII CGCG 1 cut(s) 56
AciI CCGC 1 cut(s) 54
AcsI RAATTY 1 cut(s) 332
AcyI GRCGYC 2 cut(s) 71, 83
AfiI CCNNNNNNNGG 1 cut(s) 425
AjnI CCWGG 1 cut(s) 65
AluBI AGCT 6 cut(s) 17, 32, 294, 456, 485, 540
AluI AGCT 6 cut(s) 17, 32, 294, 456, 485, 540
ApeKI GCWGC 1 cut(s) 482
ApoI RAATTY 1 cut(s) 332
ArsI GACNNNNNNTTYG 2 cut(s) 317, 349
AspS9I GGNCC 1 cut(s) 164
AvaII GGWCC 1 cut(s) 164
AxyI CCTNAGG 1 cut(s) 101
BbvI GCAGC 1 cut(s) 494
BccI CCATC 2 cut(s) 219, 481
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 1 cut(s) 67
BclI TGATCA 1 cut(s) 378
BisI GCNGC 1 cut(s) 483
BlsI GCNGC 1 cut(s) 484
Bme1390I CCNGG 1 cut(s) 67
Bme18I GGWCC 1 cut(s) 164
BmgT120I GGNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 67
BmsI GCATC 1 cut(s) 469
BpuEI CTTGAG 2 cut(s) 263, 347
BsaHI GRCGYC 2 cut(s) 71, 83
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 1 cut(s) 430
Bse21I CCTNAGG 1 cut(s) 101
Bse3DI GCAATG 1 cut(s) 74
BseBI CCWGG 1 cut(s) 67
BseGI GGATG 1 cut(s) 256
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMI GCAATG 1 cut(s) 74
BseMII CTCAG 1 cut(s) 115
BseNI ACTGG 1 cut(s) 430
BseXI GCAGC 1 cut(s) 494
Bsh1236I CGCG 1 cut(s) 56
BsiSI CCGG 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 425
Bsp143I GATC 1 cut(s) 378
BspACI CCGC 1 cut(s) 54
BspCNI CTCAG 1 cut(s) 114
BspFNI CGCG 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 161
BsrI ACTGG 1 cut(s) 430
BssAI RCCGGY 1 cut(s) 161
BssMI GATC 1 cut(s) 378
BssNI GRCGYC 2 cut(s) 71, 83
Bst2UI CCWGG 1 cut(s) 67
Bst6I CTCTTC 1 cut(s) 240
BstACI GRCGYC 2 cut(s) 71, 83
BstDEI CTNAG 1 cut(s) 101
BstF5I GGATG 1 cut(s) 256
BstFNI CGCG 1 cut(s) 56
BstKTI GATC 1 cut(s) 381
BstMBI GATC 1 cut(s) 378
BstMWI GCNNNNNNNGC 1 cut(s) 145
BstNI CCWGG 1 cut(s) 67
BstSCI CCNGG 1 cut(s) 65
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 494
Bsu36I CCTNAGG 1 cut(s) 101
BtsCI GGATG 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 164
CseI GACGC 1 cut(s) 79
CviAII CATG 1 cut(s) 352
CviJI RGCY 8 cut(s) 17, 32, 161, 294, 456, 472, 485, 540
CviKI_1 RGCY 8 cut(s) 17, 32, 161, 294, 456, 472, 485, 540
DdeI CTNAG 1 cut(s) 101
DpnI GATC 1 cut(s) 380
DpnII GATC 1 cut(s) 378
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 164
Eco81I CCTNAGG 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 65
FaeI CATG 1 cut(s) 355
FatI CATG 1 cut(s) 351
FbaI TGATCA 1 cut(s) 378
Fnu4HI GCNGC 1 cut(s) 483
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 1 cut(s) 483
GluI GCNGC 1 cut(s) 483
HapII CCGG 1 cut(s) 162
HgaI GACGC 1 cut(s) 79
Hin1I GRCGYC 2 cut(s) 71, 83
Hin1II CATG 1 cut(s) 355
HincII GTYRAC 1 cut(s) 47
HindII GTYRAC 1 cut(s) 47
HinfI GANTC 5 cut(s) 7, 24, 225, 301, 461
HpaII CCGG 1 cut(s) 162
Hpy166II GTNNAC 1 cut(s) 47
Hpy188I TCNGA 4 cut(s) 29, 239, 325, 478
Hpy188III TCNNGA 1 cut(s) 94
Hpy8I GTNNAC 1 cut(s) 47
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 1 cut(s) 510
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 1 cut(s) 482
HpyF10VI GCNNNNNNNGC 1 cut(s) 145
HpyF3I CTNAG 1 cut(s) 101
HpySE526I ACGT 1 cut(s) 83
Hsp92I GRCGYC 2 cut(s) 71, 83
Hsp92II CATG 1 cut(s) 355
Ksp22I TGATCA 1 cut(s) 378
Kzo9I GATC 1 cut(s) 378
LmnI GCTCC 3 cut(s) 29, 145, 299
LpnPI CCDG 9 cut(s) 5, 52, 79, 88, 175, 318, 411, 432, 448
Lsp1109I GCAGC 1 cut(s) 494
LweI GCATC 1 cut(s) 469
MaeII ACGT 1 cut(s) 83
MalI GATC 1 cut(s) 380
MboI GATC 1 cut(s) 378
MboII GAAGA 2 cut(s) 143, 257
MluCI AATT 4 cut(s) 134, 149, 332, 399
MlyI GAGTC 1 cut(s) 33
MmeI TCCRAC 1 cut(s) 7
MnlI CCTC 4 cut(s) 110, 218, 241, 484
MseI TTAA 3 cut(s) 174, 309, 372
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 1 cut(s) 67
MvaI CCWGG 1 cut(s) 67
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 1 cut(s) 145
NdeII GATC 1 cut(s) 378
NlaIII CATG 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PfeI GAWTC 3 cut(s) 225, 301, 461
PkrI GCNGC 1 cut(s) 484
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
Psp6I CCWGG 1 cut(s) 65
PspGI CCWGG 1 cut(s) 65
PspPI GGNCC 1 cut(s) 164
SaqAI TTAA 3 cut(s) 174, 309, 372
SatI GCNGC 1 cut(s) 483
Sau3AI GATC 1 cut(s) 378
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 1 cut(s) 67
SfaNI GCATC 1 cut(s) 469
SinI GGWCC 1 cut(s) 164
SmlI CTYRAG 2 cut(s) 278, 362
SmoI CTYRAG 2 cut(s) 278, 362
Sse9I AATT 4 cut(s) 134, 149, 332, 399
SsiI CCGC 1 cut(s) 54
StyD4I CCNGG 1 cut(s) 65
TaiI ACGT 1 cut(s) 86
TaqI TCGA 3 cut(s) 10, 95, 157
TaqII GACCGA 1 cut(s) 32
TasI AATT 4 cut(s) 134, 149, 332, 399
TfiI GAWTC 3 cut(s) 225, 301, 461
Tru1I TTAA 3 cut(s) 174, 309, 372
Tru9I TTAA 3 cut(s) 174, 309, 372
TseI GCWGC 1 cut(s) 482
TspDTI ATGAA 2 cut(s) 218, 439
TspGWI ACGGA 1 cut(s) 405
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 1 cut(s) 332
ZraI GACGTC 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.