RLG00000017787

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008898
Physical Location & Seq
Reverse (-)
21189552 .. 21191467
1916 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017787

Sequence Viewer

Length: 600 bp
ATGACGACTCGAATAGCTCCGGAAGTCGGAGCCAACTTGCTCGGTCAACACTCAGCCGAGAGAAACCAGGACGCTACTGCTTACGTCTGTAATCTCGACCCTCAGGTTAGTGAAGAATTGCTGTGGGAGCTATTTGTTCAAGCCGGTCCTGCTGTTAATGTGTATGTTCCTAAAGATAGAGTTACGAACCTCCATCAAGGATACAGATTCGTGGAGTTCCGAAGTGAAGAGGATGCTGATTACATAAGTTTTCCTTTTCTCAAGTTATTATATAGCTTCCCGATTCCTGTTAATGATGTTGTTTCTGACTATAAATTTGTTGTTTTGACTGCCATGCAGGCAATCAAGGTGCTTAATATGATCAAGCTTTACAGAAAGGCAATTCGTGTAAATAAGGGCTTCCGGGAAAGCATTCTGCTTCATCTCTCTATGCCACATATCCTTGGATGTAATGATATTCTGATTGATCGTGTGAATTCTTTGATTTCGGCTTCCCAAGATAAAAAGAGCTTGGATGTAGGAGCTAATCTTTTTGTTGGAAATCTTGACCTTGACACTGCCATCATGATCAGAGCTCCTGCACTCAGTTCTTGCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1

Protein Analysis

200

Amino Acids

22.28

Weight (kDa)

5.79

Isoelectric Point (pI)

32.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 28 - 81 2.5e-11 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 19
AcsI RAATTY 2 cut(s) 314, 475
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 1 cut(s) 140
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 8 cut(s) 17, 130, 276, 367, 510, 524, 575, 597
AluI AGCT 8 cut(s) 17, 130, 276, 367, 510, 524, 575, 597
Alw21I GWGCWC 1 cut(s) 577
Aor13HI TCCGGA 1 cut(s) 19
ApeKI GCWGC 1 cut(s) 594
ApoI RAATTY 2 cut(s) 314, 475
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
Asp700I GAANNNNTTC 1 cut(s) 411
AspS9I GGNCC 1 cut(s) 146
AsuC2I CCSGG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 146
AxyI CCTNAGG 1 cut(s) 102
BanII GRGCYC 1 cut(s) 577
Bbv12I GWGCWC 1 cut(s) 577
BccI CCATC 2 cut(s) 201, 569
BciT130I CCWGG 1 cut(s) 68
BciVI GTATCC 1 cut(s) 194
BclI TGATCA 2 cut(s) 360, 567
BcnI CCSGG 1 cut(s) 404
BfuI GTATCC 1 cut(s) 194
BglI GCCNNNNNGGC 1 cut(s) 338
BisI GCNGC 1 cut(s) 595
BlsI GCNGC 1 cut(s) 596
Bme1390I CCNGG 2 cut(s) 68, 404
Bme18I GGWCC 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 2 cut(s) 68, 404
BmsI GCATC 1 cut(s) 223
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 1 cut(s) 404
BsaJI CCNNGG 1 cut(s) 442
BsaWI WCCGGW 1 cut(s) 19
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse118I RCCGGY 1 cut(s) 143
Bse21I CCTNAGG 1 cut(s) 102
BseAI TCCGGA 1 cut(s) 19
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 442
BseGI GGATG 3 cut(s) 238, 452, 520
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMII CTCAG 3 cut(s) 66, 116, 598
BsgI GTGCAG 1 cut(s) 564
BsiHKAI GWGCWC 1 cut(s) 577
BsiSI CCGG 3 cut(s) 20, 144, 403
BslI CCNNNNNNNGG 1 cut(s) 26
BsmI GAATGC 1 cut(s) 411
Bsp1286I GDGCHC 1 cut(s) 577
Bsp13I TCCGGA 1 cut(s) 19
Bsp143I GATC 3 cut(s) 360, 466, 567
BspCNI CTCAG 3 cut(s) 65, 115, 597
BspEI TCCGGA 1 cut(s) 19
BspHI TCATGA 1 cut(s) 564
BspLI GGNNCC 1 cut(s) 31
BsrFI RCCGGY 1 cut(s) 143
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 442
BssMI GATC 3 cut(s) 360, 466, 567
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 1 cut(s) 68
Bst6I CTCTTC 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 3 cut(s) 52, 102, 584
BstF5I GGATG 3 cut(s) 238, 452, 520
BstKTI GATC 3 cut(s) 363, 469, 570
BstMBI GATC 3 cut(s) 360, 466, 567
BstMWI GCNNNNNNNGC 3 cut(s) 127, 149, 338
BstNI CCWGG 1 cut(s) 68
BstSCI CCNGG 2 cut(s) 66, 402
Bsu36I CCTNAGG 1 cut(s) 102
BsuI GTATCC 1 cut(s) 194
BtsCI GGATG 3 cut(s) 238, 452, 520
BtsI GCAGTG 1 cut(s) 555
BtsIMutI CAGTG 1 cut(s) 555
Cac8I GCNNGC 1 cut(s) 339
CciI TCATGA 1 cut(s) 564
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 146
CseI GACGC 1 cut(s) 80
CviAII CATG 2 cut(s) 334, 565
DdeI CTNAG 3 cut(s) 52, 102, 584
DpnI GATC 3 cut(s) 362, 468, 569
DpnII GATC 3 cut(s) 360, 466, 567
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
Ecl136II GAGCTC 1 cut(s) 575
Eco130I CCWWGG 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 577
Eco47I GGWCC 1 cut(s) 146
Eco53kI GAGCTC 1 cut(s) 575
Eco81I CCTNAGG 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 66
EcoT14I CCWWGG 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 577
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 2 cut(s) 337, 568
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FatI CATG 2 cut(s) 333, 564
FbaI TGATCA 2 cut(s) 360, 567
Fnu4HI GCNGC 1 cut(s) 595
FokI GGATG 3 cut(s) 245, 459, 527
FriOI GRGCYC 1 cut(s) 577
Fsp4HI GCNGC 1 cut(s) 595
GluI GCNGC 1 cut(s) 595
HapII CCGG 3 cut(s) 20, 144, 403
HgaI GACGC 1 cut(s) 80
Hin1II CATG 2 cut(s) 337, 568
HincII GTYRAC 1 cut(s) 47
HindII GTYRAC 1 cut(s) 47
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 3 cut(s) 7, 207, 283
HpaII CCGG 3 cut(s) 20, 144, 403
Hpy166II GTNNAC 1 cut(s) 47
Hpy188I TCNGA 5 cut(s) 29, 221, 307, 462, 572
Hpy188III TCNNGA 5 cut(s) 20, 95, 280, 545, 565
Hpy8I GTNNAC 1 cut(s) 47
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 3 cut(s) 337, 581, 594
HpyF10VI GCNNNNNNNGC 3 cut(s) 127, 149, 338
HpyF3I CTNAG 3 cut(s) 52, 102, 584
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 2 cut(s) 337, 568
Kpn2I TCCGGA 1 cut(s) 19
Ksp22I TGATCA 2 cut(s) 360, 567
Kzo9I GATC 3 cut(s) 360, 466, 567
LmnI GCTCC 5 cut(s) 22, 29, 127, 521, 580
LweI GCATC 1 cut(s) 223
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 181
MalI GATC 3 cut(s) 362, 468, 569
MboI GATC 3 cut(s) 360, 466, 567
MboII GAAGA 2 cut(s) 125, 239
MhlI GDGCHC 1 cut(s) 577
MluCI AATT 4 cut(s) 116, 314, 381, 475
MmeI TCCRAC 2 cut(s) 7, 517
MnlI CCTC 3 cut(s) 111, 200, 223
MroI TCCGGA 1 cut(s) 19
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 3 cut(s) 156, 291, 354
MspI CCGG 3 cut(s) 20, 144, 403
MspR9I CCNGG 2 cut(s) 68, 404
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 1 cut(s) 68
MwoI GCNNNNNNNGC 3 cut(s) 127, 149, 338
NciI CCSGG 1 cut(s) 404
NdeII GATC 3 cut(s) 360, 466, 567
NlaIII CATG 2 cut(s) 337, 568
NlaIV GGNNCC 1 cut(s) 31
NmeAIII GCCGAG 1 cut(s) 82
PagI TCATGA 1 cut(s) 564
PctI GAATGC 1 cut(s) 411
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 2 cut(s) 207, 283
PfoI TCCNGGA 1 cut(s) 402
PkrI GCNGC 1 cut(s) 596
Psp124BI GAGCTC 1 cut(s) 577
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 1 cut(s) 146
SacI GAGCTC 1 cut(s) 577
SaqAI TTAA 3 cut(s) 156, 291, 354
SatI GCNGC 1 cut(s) 595
Sau3AI GATC 3 cut(s) 360, 466, 567
Sau96I GGNCC 1 cut(s) 146
ScrFI CCNGG 2 cut(s) 68, 404
SduI GDGCHC 1 cut(s) 577
SfaNI GCATC 1 cut(s) 223
SinI GGWCC 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 4 cut(s) 116, 314, 381, 475
SstI GAGCTC 1 cut(s) 577
StyD4I CCNGG 2 cut(s) 66, 402
StyI CCWWGG 1 cut(s) 442
TaiI ACGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 10, 96
TaqII GACCGA 1 cut(s) 32
TasI AATT 4 cut(s) 116, 314, 381, 475
TfiI GAWTC 2 cut(s) 207, 283
Tru1I TTAA 3 cut(s) 156, 291, 354
Tru9I TTAA 3 cut(s) 156, 291, 354
TscAI CASTG 1 cut(s) 562
TseI GCWGC 1 cut(s) 594
TspDTI ATGAA 1 cut(s) 410
TspRI CASTG 1 cut(s) 562
VpaK11BI GGWCC 1 cut(s) 146
XapI RAATTY 2 cut(s) 314, 475
XmnI GAANNNNTTC 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.