RLG00000030135

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008898
Physical Location & Seq
Reverse (-)
56177760 .. 56181706
3947 bp
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UTR
Exon/CDS
Intron
RLM00000030135

Sequence Viewer

Length: 426 bp
ATGACGACTCGAACAGCTCCGGGAGTCGGAGCCAACTTGCTCGGTCAACACTCCGCCGAGAGAAACCAGGACGCCACTGCCTACGTCTGTAATCTCGACCCTCAGGTTAGTGAAGAATTGCTGTGGGAGCTATTTGTTCAAGCCGGTCGTGCTGTTAATGTGTATGTTCCTAAAGATAGAGTTACGAACCTCCATCAAGGATACAGATTCGTGGAGTTCCGAAGTGAAGAGGATGCTGATTACATAAGTTTTCCTTTTCTCAAGTTATTATATAGCTCCCCGATTCCTGTTAAAGATGTTGTTTCTGACTATAAATTTGTTGTTTTGACTGCCATGCCGGCAATCAAGGTGCTTAATATGATCAAGCTTTACAGAAAGGCAATTCGTGTAAATAAGCTTCCCAAGATAAAAAGAGCTTGGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1

Protein Analysis

142

Amino Acids

16.13

Weight (kDa)

9.38

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 28 - 81 3.5e-11 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 54
AcsI RAATTY 1 cut(s) 314
AcyI GRCGYC 1 cut(s) 72
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 1 cut(s) 140
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 6 cut(s) 17, 130, 276, 367, 397, 416
AluI AGCT 6 cut(s) 17, 130, 276, 367, 397, 416
ApoI RAATTY 1 cut(s) 314
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
AsuC2I CCSGG 1 cut(s) 21
AxyI CCTNAGG 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 381, 413
BccI CCATC 1 cut(s) 201
BciT130I CCWGG 1 cut(s) 68
BciVI GTATCC 1 cut(s) 194
BclI TGATCA 1 cut(s) 360
BcnI CCSGG 1 cut(s) 21
BfuI GTATCC 1 cut(s) 194
BglI GCCNNNNNGGC 1 cut(s) 338
Bme1390I CCNGG 2 cut(s) 21, 68
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 2 cut(s) 21, 68
BmsI GCATC 1 cut(s) 223
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 72
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse118I RCCGGY 2 cut(s) 143, 337
Bse21I CCTNAGG 1 cut(s) 102
BseBI CCWGG 1 cut(s) 68
BseGI GGATG 2 cut(s) 238, 426
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMII CTCAG 1 cut(s) 116
Bsh1285I CGRYCG 1 cut(s) 148
BsiEI CGRYCG 1 cut(s) 148
BsiSI CCGG 3 cut(s) 20, 144, 338
BslI CCNNNNNNNGG 1 cut(s) 26
Bsp143I GATC 1 cut(s) 360
BspACI CCGC 1 cut(s) 54
BspCNI CTCAG 1 cut(s) 115
BspLI GGNNCC 1 cut(s) 31
BsrFI RCCGGY 2 cut(s) 143, 337
BssAI RCCGGY 2 cut(s) 143, 337
BssMI GATC 1 cut(s) 360
BssNI GRCGYC 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 68
Bst6I CTCTTC 1 cut(s) 222
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 1 cut(s) 102
BstF5I GGATG 2 cut(s) 238, 426
BstKTI GATC 1 cut(s) 363
BstMBI GATC 1 cut(s) 360
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 3 cut(s) 127, 149, 338
BstNI CCWGG 1 cut(s) 68
BstSCI CCNGG 2 cut(s) 19, 66
Bsu36I CCTNAGG 1 cut(s) 102
BsuI GTATCC 1 cut(s) 194
BtsCI GGATG 2 cut(s) 238, 426
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 339
Cfr10I RCCGGY 2 cut(s) 143, 337
CseI GACGC 1 cut(s) 80
CviAII CATG 1 cut(s) 334
CviJI RGCY 8 cut(s) 17, 32, 130, 143, 276, 367, 397, 416
CviKI_1 RGCY 8 cut(s) 17, 32, 130, 143, 276, 367, 397, 416
DdeI CTNAG 1 cut(s) 102
DpnI GATC 1 cut(s) 362
DpnII GATC 1 cut(s) 360
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
EciI GGCGGA 1 cut(s) 43
Eco81I CCTNAGG 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 66
FaeI CATG 1 cut(s) 337
FaiI YATR 7 cut(s) 165, 245, 271, 273, 312, 335, 359
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FatI CATG 1 cut(s) 333
FbaI TGATCA 1 cut(s) 360
FokI GGATG 1 cut(s) 245
HapII CCGG 3 cut(s) 20, 144, 338
HgaI GACGC 1 cut(s) 80
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 1 cut(s) 337
HincII GTYRAC 1 cut(s) 47
HindII GTYRAC 1 cut(s) 47
HindIII AAGCTT 2 cut(s) 365, 395
HinfI GANTC 4 cut(s) 7, 24, 207, 283
HpaII CCGG 3 cut(s) 20, 144, 338
Hpy166II GTNNAC 1 cut(s) 47
Hpy188I TCNGA 3 cut(s) 29, 221, 307
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 1 cut(s) 47
HpyCH4IV ACGT 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 3 cut(s) 127, 149, 338
HpyF3I CTNAG 1 cut(s) 102
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 1 cut(s) 337
KroI GCCGGC 1 cut(s) 337
KroNI GCCGGC 1 cut(s) 339
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 1 cut(s) 360
LmnI GCTCC 4 cut(s) 22, 29, 127, 281
LpnPI CCDG 7 cut(s) 33, 53, 80, 89, 157, 300, 351
LweI GCATC 1 cut(s) 223
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 181
MalI GATC 1 cut(s) 362
MboI GATC 1 cut(s) 360
MboII GAAGA 2 cut(s) 125, 239
MluCI AATT 3 cut(s) 116, 314, 381
MlyI GAGTC 1 cut(s) 33
MmeI TCCRAC 1 cut(s) 7
MnlI CCTC 3 cut(s) 111, 200, 223
MroNI GCCGGC 1 cut(s) 337
MseI TTAA 3 cut(s) 156, 291, 354
MspI CCGG 3 cut(s) 20, 144, 338
MspR9I CCNGG 2 cut(s) 21, 68
MvaI CCWGG 1 cut(s) 68
MwoI GCNNNNNNNGC 3 cut(s) 127, 149, 338
NaeI GCCGGC 1 cut(s) 339
NciI CCSGG 1 cut(s) 21
NdeII GATC 1 cut(s) 360
NgoMIV GCCGGC 1 cut(s) 337
NlaIII CATG 1 cut(s) 337
NlaIV GGNNCC 1 cut(s) 31
NmeAIII GCCGAG 1 cut(s) 82
PdiI GCCGGC 1 cut(s) 339
PfeI GAWTC 2 cut(s) 207, 283
PfoI TCCNGGA 1 cut(s) 19
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 31
SaqAI TTAA 3 cut(s) 156, 291, 354
Sau3AI GATC 1 cut(s) 360
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 2 cut(s) 21, 68
SfaNI GCATC 1 cut(s) 223
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 3 cut(s) 116, 314, 381
SsiI CCGC 1 cut(s) 54
StyD4I CCNGG 2 cut(s) 19, 66
TaiI ACGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 10, 96
TaqII GACCGA 1 cut(s) 32
TasI AATT 3 cut(s) 116, 314, 381
TfiI GAWTC 2 cut(s) 207, 283
Tru1I TTAA 3 cut(s) 156, 291, 354
Tru9I TTAA 3 cut(s) 156, 291, 354
TscAI CASTG 1 cut(s) 82
TspRI CASTG 1 cut(s) 82
XapI RAATTY 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.