RLG00000014192

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008898
Physical Location & Seq
Reverse (-)
49024880 .. 49034541
9662 bp
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UTR
Exon/CDS
Intron
RLM00000014192

Sequence Viewer

Length: 672 bp
ATGACGACTCGAATAGCTCCGGAAGTCGGAGCCAACTTGCTCGGTCAACACTCAGCCGAGAGAAACCAGGACGCTACTGCTTACGTCTGTAATCTCGACCCTCAGGTTAGTGAAGAATTGCTGTGGGAGCTATTTGTTCAAGCCGGTCCTGCTGTTAATGTGTATGTTCCCAAAGATAGAGTTACGAACCTCCATCAAGGATACAGATTCATGGAGTTCCGAAGTGAAGAGGATGCTGATTACATAAGTTTTCCTTTTCTCAAGTTATTATATAGCTCCCCGATTCCTGTTAATGATGTTGTTTCTGACTATAAATTTGTTGTTTTGACTGCCATGCAGGCAATCAAGGTGCTTAATATGATCAAGCTTTACAGAAAGGCAATTCGTGTAAATAAGGGCTTCCGGGAAAGCATTCTGCTTCATCTCTCTATGCCACATATCCTTGGATGTAATGATATTCTGATTGATCGTGTGAATTCTTTGATTTCGGCTTCCCAAGATAAAAAGAGCTTGGATGTAGGAGCTAATCTTTTTGTTGGAAATCTTGACCTTTGGAAACTAAGGCCACCCGGAGTTCCTCAGTTGCTTCTTCTACGACTTCTTTGGGAGCTCGAAGAAGACGGTGTTCGTTTGGACAGAGAGCAAGCGACGACTGCCATGAAGAGAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1

Protein Analysis

224

Amino Acids

25.3

Weight (kDa)

6.11

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 28 - 81 3.2e-10 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 19
AcsI RAATTY 2 cut(s) 314, 475
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 1 cut(s) 140
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 7 cut(s) 17, 130, 276, 367, 510, 524, 610
AluI AGCT 7 cut(s) 17, 130, 276, 367, 510, 524, 610
Alw21I GWGCWC 1 cut(s) 612
Aor13HI TCCGGA 1 cut(s) 19
AoxI GGCC 1 cut(s) 563
ApoI RAATTY 2 cut(s) 314, 475
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
Asp700I GAANNNNTTC 1 cut(s) 411
AspS9I GGNCC 1 cut(s) 146
AsuC2I CCSGG 2 cut(s) 404, 570
AvaII GGWCC 1 cut(s) 146
AxyI CCTNAGG 1 cut(s) 102
BanII GRGCYC 1 cut(s) 612
BbsI GAAGAC 1 cut(s) 624
Bbv12I GWGCWC 1 cut(s) 612
BccI CCATC 1 cut(s) 201
BciT130I CCWGG 1 cut(s) 68
BciVI GTATCC 1 cut(s) 194
BclI TGATCA 1 cut(s) 360
BcnI CCSGG 2 cut(s) 404, 570
BfaI CTAG 1 cut(s) 670
BfuI GTATCC 1 cut(s) 194
BglI GCCNNNNNGGC 1 cut(s) 338
Bme1390I CCNGG 3 cut(s) 68, 404, 570
Bme18I GGWCC 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 3 cut(s) 68, 404, 570
BmsI GCATC 1 cut(s) 223
BpiI GAAGAC 1 cut(s) 624
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 2 cut(s) 404, 570
BsaJI CCNNGG 1 cut(s) 442
BsaWI WCCGGW 1 cut(s) 19
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse118I RCCGGY 1 cut(s) 143
Bse21I CCTNAGG 1 cut(s) 102
BseAI TCCGGA 1 cut(s) 19
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 442
BseGI GGATG 3 cut(s) 238, 452, 520
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMII CTCAG 3 cut(s) 66, 116, 593
BshFI GGCC 1 cut(s) 565
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 4 cut(s) 20, 144, 403, 570
BslI CCNNNNNNNGG 1 cut(s) 26
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 1 cut(s) 565
Bsp1286I GDGCHC 1 cut(s) 612
Bsp13I TCCGGA 1 cut(s) 19
Bsp143I GATC 2 cut(s) 360, 466
BspANI GGCC 1 cut(s) 565
BspCNI CTCAG 3 cut(s) 65, 115, 592
BspEI TCCGGA 1 cut(s) 19
BspLI GGNNCC 1 cut(s) 31
BsrFI RCCGGY 1 cut(s) 143
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 442
BssMI GATC 2 cut(s) 360, 466
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 1 cut(s) 623
Bst6I CTCTTC 2 cut(s) 222, 656
BstC8I GCNNGC 2 cut(s) 339, 645
BstDEI CTNAG 4 cut(s) 52, 102, 560, 579
BstF5I GGATG 3 cut(s) 238, 452, 520
BstKTI GATC 2 cut(s) 363, 469
BstMBI GATC 2 cut(s) 360, 466
BstMWI GCNNNNNNNGC 4 cut(s) 127, 149, 338, 653
BstNI CCWGG 1 cut(s) 68
BstSCI CCNGG 3 cut(s) 66, 402, 568
BstV2I GAAGAC 1 cut(s) 624
Bsu36I CCTNAGG 1 cut(s) 102
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 1 cut(s) 565
BtsCI GGATG 3 cut(s) 238, 452, 520
Cac8I GCNNGC 2 cut(s) 339, 645
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 146
CseI GACGC 1 cut(s) 80
CviAII CATG 3 cut(s) 211, 334, 658
DdeI CTNAG 4 cut(s) 52, 102, 560, 579
DpnI GATC 2 cut(s) 362, 468
DpnII GATC 2 cut(s) 360, 466
Eam1104I CTCTTC 2 cut(s) 222, 656
EarI CTCTTC 2 cut(s) 222, 656
Ecl136II GAGCTC 1 cut(s) 610
Eco130I CCWWGG 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 612
Eco47I GGWCC 1 cut(s) 146
Eco53kI GAGCTC 1 cut(s) 610
Eco81I CCTNAGG 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 610
EcoRI GAATTC 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 66
EcoT14I CCWWGG 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 612
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 3 cut(s) 214, 337, 661
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FatI CATG 3 cut(s) 210, 333, 657
FbaI TGATCA 1 cut(s) 360
FokI GGATG 3 cut(s) 245, 459, 527
FriOI GRGCYC 1 cut(s) 612
FspBI CTAG 1 cut(s) 670
HaeIII GGCC 1 cut(s) 565
HapII CCGG 4 cut(s) 20, 144, 403, 570
HgaI GACGC 1 cut(s) 80
Hin1II CATG 3 cut(s) 214, 337, 661
HincII GTYRAC 1 cut(s) 47
HindII GTYRAC 1 cut(s) 47
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 3 cut(s) 7, 207, 283
HpaII CCGG 4 cut(s) 20, 144, 403, 570
Hpy166II GTNNAC 1 cut(s) 47
Hpy188I TCNGA 4 cut(s) 29, 221, 307, 462
Hpy188III TCNNGA 3 cut(s) 20, 95, 545
Hpy8I GTNNAC 1 cut(s) 47
Hpy99I CGWCG 1 cut(s) 652
HpyCH4III ACNGT 1 cut(s) 623
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 1 cut(s) 337
HpyF10VI GCNNNNNNNGC 4 cut(s) 127, 149, 338, 653
HpyF3I CTNAG 4 cut(s) 52, 102, 560, 579
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 3 cut(s) 214, 337, 661
Kpn2I TCCGGA 1 cut(s) 19
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 2 cut(s) 360, 466
LmnI GCTCC 6 cut(s) 22, 29, 127, 281, 521, 607
LweI GCATC 1 cut(s) 223
MaeI CTAG 1 cut(s) 670
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 181
MalI GATC 2 cut(s) 362, 468
MboI GATC 2 cut(s) 360, 466
MboII GAAGA 5 cut(s) 125, 239, 581, 626, 629
MhlI GDGCHC 1 cut(s) 612
MluCI AATT 4 cut(s) 116, 314, 381, 475
MmeI TCCRAC 2 cut(s) 7, 517
MnlI CCTC 5 cut(s) 111, 200, 223, 588, 659
MroI TCCGGA 1 cut(s) 19
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 3 cut(s) 156, 291, 354
MspI CCGG 4 cut(s) 20, 144, 403, 570
MspR9I CCNGG 3 cut(s) 68, 404, 570
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 1 cut(s) 68
MwoI GCNNNNNNNGC 4 cut(s) 127, 149, 338, 653
NciI CCSGG 2 cut(s) 404, 570
NdeII GATC 2 cut(s) 360, 466
NlaIII CATG 3 cut(s) 214, 337, 661
NlaIV GGNNCC 1 cut(s) 31
NmeAIII GCCGAG 1 cut(s) 82
PctI GAATGC 1 cut(s) 411
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 2 cut(s) 207, 283
PfoI TCCNGGA 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 612
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 1 cut(s) 146
SacI GAGCTC 1 cut(s) 612
SaqAI TTAA 3 cut(s) 156, 291, 354
Sau3AI GATC 2 cut(s) 360, 466
Sau96I GGNCC 1 cut(s) 146
ScrFI CCNGG 3 cut(s) 68, 404, 570
SduI GDGCHC 1 cut(s) 612
SfaNI GCATC 1 cut(s) 223
SinI GGWCC 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 4 cut(s) 116, 314, 381, 475
SspMI CTAG 1 cut(s) 670
SstI GAGCTC 1 cut(s) 612
StyD4I CCNGG 3 cut(s) 66, 402, 568
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 1 cut(s) 623
TaiI ACGT 1 cut(s) 87
TaqI TCGA 3 cut(s) 10, 96, 612
TaqII GACCGA 1 cut(s) 32
TasI AATT 4 cut(s) 116, 314, 381, 475
TfiI GAWTC 2 cut(s) 207, 283
Tru1I TTAA 3 cut(s) 156, 291, 354
Tru9I TTAA 3 cut(s) 156, 291, 354
TspDTI ATGAA 2 cut(s) 199, 410
VpaK11BI GGWCC 1 cut(s) 146
XapI RAATTY 2 cut(s) 314, 475
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.