RchiOBHm_Chr5g0043771

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010590
Physical Location & Seq
Reverse (-)
38811092 .. 38813345
2254 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32200

Sequence Viewer

Length: 1209 bp
ATGAGCACTCTATGCCAGGAAAAGAAAGCCTTCATTCCCGACGATCATCGCTCTCTATACCTCTGCTTCGGTGAGTACGAAGAGGGCAGCAAGGTGTCCTACACCATTTTATCAGCCAGCTTGCACCAATTGTTTTATGCTTGTTCTTCTCCACGGCAATTACAGCTAGTTACTAGGTTTTCCGGCGAAGGTCTTCCCCGCTATGTGGGTTGCGGCCTTTGGGGCTCCAAAATTCTCCTCGGGGGTGGCGCAAAACCAGCTTATTTGCATTGTCCGAGTCGAGGCCAGCCTGCTAGTGGGTGTTTTATACATCACGAGGTCAGAAAGATATACTGTTTTGAGACTACGGAGGAGACTCAGGTACCCATGCCACTCCCGAAGAAGATGTTGAAAGGGAACATTCAACCCTTGCTAGTGGAGGTGAACGGCAAACTCTACGCTCTTGGTAAATCATTACCGTCTTTTGAAATGTTTGACGGCAATGATTGGTCTGAGTTGGCACCACCTCCACACGGAACTTATTTGTGTTATCCCTTTACACATTCAGACCCAGCTTGTCAGTTCGATGTATACTATGCAGTTGCCGGCACAAATATATTAGTTTCTGATGGTGGTTCAAGCTTCCGCTTTGACACAAGTGACCCCAAGCAAGGCTGGAGAAGACTAATATCAGCCGACCAATTCTCTTTCGACCCATTCTGTTTTAAGGGCACAAGTGTAGTTGTGAATGATGGTAAACAGTTTGTGCTTTTCACATTCCAGCCCTCTGATGGTCAGCATATATCAGTGCATCTGATTGCATATGACTTTGATAGCATCACACAAGACTACAGTCGTGTGCCGCTGGCTCCATACTTGATGGAATACAAGGATGATCGTCTTGGTGATCTTCCATTACCTAATTATCGGCTAGTACACATAGGAGATAACGCTGTCTGCCTTCTTATGTCTAAACTCCTCCCCAATGGAGTGGATGAGAGGAAGAAGAAGATGATTAGGTGTGTTGAATTCGCAGCATTCACATTCAAAGTCAGAGACAATACTAGACTATCCTTAAAAATCAGATTCCTGCCTCATACCTCAAAGCTCATATATGATGATGACAATGATGATGATATGGACATGGCTTCTAGGACTGGACAGATGCTTGGTGCCTTCTTGTTGGAAAATTCTCAAGTGAACAAGGAGGACCTTGCAGCAGAACATTAG

Protein Analysis

402

Amino Acids

45.28

Weight (kDa)

5.95

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 110 - 316 1.1e-06 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 361
AccB1I GGYRCC 3 cut(s) 361, 499, 1151
AccI GTMKAC 1 cut(s) 570
AciI CCGC 4 cut(s) 199, 213, 625, 842
AcsI RAATTY 3 cut(s) 231, 1007, 1168
AfaI GTAC 3 cut(s) 77, 363, 915
AfiI CCNNNNNNNGG 6 cut(s) 205, 281, 296, 512, 650, 770
AgsI TTSAA 6 cut(s) 391, 404, 467, 618, 1007, 1027
AjnI CCWGG 1 cut(s) 15
AluBI AGCT 6 cut(s) 120, 166, 260, 554, 621, 1087
AluI AGCT 6 cut(s) 120, 166, 260, 554, 621, 1087
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 3 cut(s) 335, 347, 1029
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 2 cut(s) 214, 283
ApeKI GCWGC 3 cut(s) 87, 1013, 1196
ApoI RAATTY 3 cut(s) 231, 1007, 1168
Asp700I GAANNNNTTC 2 cut(s) 29, 192
Asp718I GGTACC 1 cut(s) 361
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 1189
AsuHPI GGTGA 3 cut(s) 83, 433, 896
AvaI CYCGRG 1 cut(s) 239
AvaII GGWCC 1 cut(s) 1189
BaeGI GKGCMC 1 cut(s) 713
BaeI ACNNNNGTAYC 2 cut(s) 345, 378
BanI GGYRCC 3 cut(s) 361, 499, 1151
BanII GRGCYC 1 cut(s) 227
BauI CACGAG 1 cut(s) 314
BbsI GAAGAC 2 cut(s) 185, 667
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 2 cut(s) 99, 1025
BccI CCATC 4 cut(s) 602, 725, 764, 853
BceAI ACGGC 3 cut(s) 170, 442, 493
BciT130I CCWGG 1 cut(s) 17
BcoDI GTCTC 3 cut(s) 335, 347, 1029
BfaI CTAG 7 cut(s) 167, 174, 294, 413, 911, 1044, 1131
BfmI CTRYAG 1 cut(s) 829
BglI GCCNNNNNGGC 1 cut(s) 222
BisI GCNGC 5 cut(s) 88, 214, 842, 1014, 1197
BlsI GCNGC 5 cut(s) 89, 215, 843, 1015, 1198
Bme1390I CCNGG 1 cut(s) 17
Bme18I GGWCC 1 cut(s) 1189
BmeT110I CYCGRG 1 cut(s) 239
BmgT120I GGNCC 1 cut(s) 1189
BmiI GGNNCC 5 cut(s) 226, 363, 501, 849, 1153
BmrFI CCNGG 1 cut(s) 17
BmsI GCATC 3 cut(s) 799, 825, 1134
BoxI GACNNNNGTC 1 cut(s) 831
BpiI GAAGAC 2 cut(s) 185, 667
BpmI CTGGAG 1 cut(s) 676
BpuEI CTTGAG 1 cut(s) 1158
BsaJI CCNNGG 2 cut(s) 152, 238
Bsc4I CCNNNNNNNGG 6 cut(s) 205, 281, 296, 512, 650, 770
Bse118I RCCGGY 1 cut(s) 584
Bse1I ACTGG 1 cut(s) 1141
Bse3DI GCAATG 1 cut(s) 487
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 152, 238
BseGI GGATG 2 cut(s) 877, 979
BseLI CCNNNNNNNGG 6 cut(s) 205, 281, 296, 512, 650, 770
BseMI GCAATG 1 cut(s) 487
BseMII CTCAG 2 cut(s) 371, 483
BseNI ACTGG 1 cut(s) 1141
BseRI GAGGAG 3 cut(s) 227, 365, 947
BseSI GKGCMC 1 cut(s) 713
BseXI GCAGC 2 cut(s) 99, 1025
BseYI CCCAGC 1 cut(s) 550
BshFI GGCC 2 cut(s) 216, 285
BshNI GGYRCC 3 cut(s) 361, 499, 1151
BsiHKAI GWGCWC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 2 cut(s) 183, 585
BslI CCNNNNNNNGG 6 cut(s) 205, 281, 296, 512, 650, 770
BsmAI GTCTC 3 cut(s) 335, 347, 1029
BsmI GAATGC 1 cut(s) 1016
BsnI GGCC 2 cut(s) 216, 285
BsoBI CYCGRG 1 cut(s) 239
Bsp1286I GDGCHC 3 cut(s) 8, 227, 713
Bsp143I GATC 3 cut(s) 43, 874, 886
BspACI CCGC 4 cut(s) 199, 213, 625, 842
BspANI GGCC 2 cut(s) 216, 285
BspCNI CTCAG 2 cut(s) 370, 484
BspLI GGNNCC 5 cut(s) 226, 363, 501, 849, 1153
BspT107I GGYRCC 3 cut(s) 361, 499, 1151
BsrDI GCAATG 1 cut(s) 487
BsrFI RCCGGY 1 cut(s) 584
BsrI ACTGG 1 cut(s) 1141
BssAI RCCGGY 1 cut(s) 584
BssECI CCNNGG 2 cut(s) 152, 238
BssMI GATC 3 cut(s) 43, 874, 886
BssNAI GTATAC 1 cut(s) 571
BssSI CACGAG 1 cut(s) 314
Bst1107I GTATAC 1 cut(s) 571
Bst2BI CACGAG 1 cut(s) 314
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 4 cut(s) 335, 459, 741, 833
Bst6I CTCTTC 1 cut(s) 75
BstAPI GCANNNNNTGC 1 cut(s) 12
BstC8I GCNNGC 6 cut(s) 118, 122, 287, 291, 586, 846
BstDEI CTNAG 2 cut(s) 357, 492
BstDSI CCRYGG 1 cut(s) 152
BstF5I GGATG 2 cut(s) 877, 979
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 3 cut(s) 46, 877, 889
BstMAI GTCTC 3 cut(s) 335, 347, 1029
BstMBI GATC 3 cut(s) 43, 874, 886
BstMWI GCNNNNNNNGC 4 cut(s) 12, 163, 222, 257
BstNI CCWGG 1 cut(s) 17
BstPAI GACNNNNGTC 1 cut(s) 831
BstSCI CCNGG 1 cut(s) 15
BstSFI CTRYAG 1 cut(s) 829
BstSLI GKGCMC 1 cut(s) 713
BstV1I GCAGC 2 cut(s) 99, 1025
BstV2I GAAGAC 2 cut(s) 185, 667
BstXI CCANNNNNNTGG 1 cut(s) 970
BstZ17I GTATAC 1 cut(s) 571
BsuRI GGCC 2 cut(s) 216, 285
BtgI CCRYGG 1 cut(s) 152
BtgZI GCGATG 1 cut(s) 32
BtsCI GGATG 2 cut(s) 877, 979
BtsIMutI CAGTG 1 cut(s) 792
Cac8I GCNNGC 6 cut(s) 118, 122, 287, 291, 586, 846
CfoI GCGC 1 cut(s) 251
Cfr10I RCCGGY 1 cut(s) 584
Cfr13I GGNCC 1 cut(s) 1189
Csp6I GTAC 3 cut(s) 76, 362, 914
CviAII CATG 2 cut(s) 367, 1123
CviQI GTAC 3 cut(s) 76, 362, 914
DdeI CTNAG 2 cut(s) 357, 492
DpnI GATC 3 cut(s) 45, 876, 888
DpnII GATC 3 cut(s) 43, 874, 886
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 1189
Eco88I CYCGRG 1 cut(s) 239
EcoO109I RGGNCCY 1 cut(s) 1189
EcoRI GAATTC 1 cut(s) 1007
EcoRII CCWGG 1 cut(s) 15
EcoT38I GRGCYC 1 cut(s) 227
FaeI CATG 2 cut(s) 370, 1126
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FatI CATG 2 cut(s) 366, 1122
FauI CCCGC 1 cut(s) 206
FauNDI CATATG 1 cut(s) 802
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 5 cut(s) 88, 214, 842, 1014, 1197
FokI GGATG 2 cut(s) 884, 986
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 5 cut(s) 88, 214, 842, 1014, 1197
FspBI CTAG 7 cut(s) 167, 174, 294, 413, 911, 1044, 1131
GlaI GCGC 1 cut(s) 250
GluI GCNGC 5 cut(s) 88, 214, 842, 1014, 1197
GsaI CCCAGC 1 cut(s) 554
GsuI CTGGAG 1 cut(s) 676
HaeIII GGCC 2 cut(s) 216, 285
HapII CCGG 2 cut(s) 183, 585
HhaI GCGC 1 cut(s) 251
Hin1II CATG 2 cut(s) 370, 1126
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HindIII AAGCTT 1 cut(s) 619
HinfI GANTC 3 cut(s) 277, 355, 1065
HpaII CCGG 2 cut(s) 183, 585
HphI GGTGA 3 cut(s) 83, 433, 896
Hpy166II GTNNAC 5 cut(s) 424, 571, 737, 916, 1180
Hpy188I TCNGA 9 cut(s) 276, 323, 493, 547, 607, 769, 795, 1034, 1064
Hpy188III TCNNGA 3 cut(s) 38, 314, 376
Hpy8I GTNNAC 5 cut(s) 424, 571, 737, 916, 1180
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 4 cut(s) 40, 182, 950, 1165
HpyCH4III ACNGT 4 cut(s) 335, 459, 741, 833
HpyCH4V TGCA 6 cut(s) 124, 268, 578, 790, 800, 1196
HpyF10VI GCNNNNNNNGC 4 cut(s) 12, 163, 222, 257
HpyF3I CTNAG 2 cut(s) 357, 492
Hsp92II CATG 2 cut(s) 370, 1126
HspAI GCGC 1 cut(s) 249
KpnI GGTACC 1 cut(s) 365
KroI GCCGGC 1 cut(s) 584
KroNI GCCGGC 1 cut(s) 586
Kzo9I GATC 3 cut(s) 43, 874, 886
LmnI GCTCC 2 cut(s) 230, 853
Lsp1109I GCAGC 2 cut(s) 99, 1025
LweI GCATC 3 cut(s) 799, 825, 1134
MaeI CTAG 7 cut(s) 167, 174, 294, 413, 911, 1044, 1131
MaeIII GTNAC 2 cut(s) 169, 638
MalI GATC 3 cut(s) 45, 876, 888
MboI GATC 3 cut(s) 43, 874, 886
MfeI CAATTG 1 cut(s) 128
MhlI GDGCHC 3 cut(s) 8, 227, 713
MluCI AATT 7 cut(s) 128, 158, 231, 680, 901, 1007, 1168
MlyI GAGTC 2 cut(s) 286, 349
MmeI TCCRAC 1 cut(s) 1143
MroNI GCCGGC 1 cut(s) 584
MroXI GAANNNNTTC 2 cut(s) 29, 192
MseI TTAA 2 cut(s) 705, 1055
MspA1I CMGCKG 1 cut(s) 844
MspI CCGG 2 cut(s) 183, 585
MspR9I CCNGG 1 cut(s) 17
MunI CAATTG 1 cut(s) 128
Mva1269I GAATGC 1 cut(s) 1016
MvaI CCWGG 1 cut(s) 17
MwoI GCNNNNNNNGC 4 cut(s) 12, 163, 222, 257
NaeI GCCGGC 1 cut(s) 586
NdeI CATATG 1 cut(s) 802
NdeII GATC 3 cut(s) 43, 874, 886
NgoMIV GCCGGC 1 cut(s) 584
NlaIII CATG 2 cut(s) 370, 1126
NlaIV GGNNCC 5 cut(s) 226, 363, 501, 849, 1153
NmuCI GTSAC 1 cut(s) 638
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 1016
PdiI GCCGGC 1 cut(s) 586
PdmI GAANNNNTTC 2 cut(s) 29, 192
PfeI GAWTC 1 cut(s) 1065
PkrI GCNGC 5 cut(s) 89, 215, 843, 1015, 1198
PleI GAGTC 2 cut(s) 285, 349
PpsI GAGTC 2 cut(s) 285, 349
PpuMI RGGWCCY 1 cut(s) 1189
PshAI GACNNNNGTC 1 cut(s) 831
Psp5II RGGWCCY 1 cut(s) 1189
Psp6I CCWGG 1 cut(s) 15
PspFI CCCAGC 1 cut(s) 550
PspGI CCWGG 1 cut(s) 15
PspN4I GGNNCC 5 cut(s) 226, 363, 501, 849, 1153
PspPI GGNCC 1 cut(s) 1189
PspPPI RGGWCCY 1 cut(s) 1189
RsaI GTAC 3 cut(s) 77, 363, 915
RsaNI GTAC 3 cut(s) 76, 362, 914
SaqAI TTAA 2 cut(s) 705, 1055
SatI GCNGC 5 cut(s) 88, 214, 842, 1014, 1197
Sau3AI GATC 3 cut(s) 43, 874, 886
Sau96I GGNCC 1 cut(s) 1189
SchI GAGTC 2 cut(s) 286, 349
ScrFI CCNGG 1 cut(s) 17
SduI GDGCHC 3 cut(s) 8, 227, 713
SfaNI GCATC 3 cut(s) 799, 825, 1134
SfcI CTRYAG 1 cut(s) 829
SinI GGWCC 1 cut(s) 1189
SmlI CTYRAG 1 cut(s) 1173
SmoI CTYRAG 1 cut(s) 1173
Sse9I AATT 7 cut(s) 128, 158, 231, 680, 901, 1007, 1168
SsiI CCGC 4 cut(s) 199, 213, 625, 842
SspMI CTAG 7 cut(s) 167, 174, 294, 413, 911, 1044, 1131
StyD4I CCNGG 1 cut(s) 15
TaaI ACNGT 4 cut(s) 335, 459, 741, 833
TaqI TCGA 3 cut(s) 280, 564, 690
TasI AATT 7 cut(s) 128, 158, 231, 680, 901, 1007, 1168
TatI WGTACW 1 cut(s) 913
TauI GCSGC 2 cut(s) 216, 844
TfiI GAWTC 1 cut(s) 1065
Tru1I TTAA 2 cut(s) 705, 1055
Tru9I TTAA 2 cut(s) 705, 1055
TscAI CASTG 1 cut(s) 792
TseFI GTSAC 1 cut(s) 638
TseI GCWGC 3 cut(s) 87, 1013, 1196
Tsp45I GTSAC 1 cut(s) 638
TspDTI ATGAA 1 cut(s) 22
TspGWI ACGGA 2 cut(s) 362, 528
TspRI CASTG 1 cut(s) 792
VpaK11BI GGWCC 1 cut(s) 1189
XapI RAATTY 3 cut(s) 231, 1007, 1168
XcmI CCANNNNNNNNNTGG 2 cut(s) 293, 767
XmiI GTMKAC 1 cut(s) 570
XmnI GAANNNNTTC 2 cut(s) 29, 192
XspI CTAG 7 cut(s) 167, 174, 294, 413, 911, 1044, 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.