Rorug05G0208300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010590
Physical Location & Seq
Reverse (-)
20756721 .. 20758357
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0208300.1

Sequence Viewer

Length: 1437 bp
ATGGATTCGGAGTCAAAGCGTCAACAACAAGGAGTTGATAGAATTAGTGCATTGCCAGATGCACTTGTTTGCCACATACTGTCATTCCTTCCTACAATATATGCTGTGTGGACCACCCTTCTGTCTAGGAGATGGAACAAGTTATGGAGGTCTCTTCGCAATTTGGACTTCAATGACGTAGACTTCCCTACCAAGAGGACTGATCGTTTTATGAAATTTGTTGGTTGTGTACTTTCCTTCCGTGGCTCATCAGACATTCGAAAAATCTGTCTTCACATCCACCATTGTGAGAACTTCAGTACTGGGGATTTCTCTCGCGTTCAGAGGTGGATTCGTGCTGCCATTAGGCATAATATTGTTGAACTTGATCTTTCTCTTTGGTTTCAGTATCGCAAGAATGATGAGTTTCAAATGCCTCTAAGCCTTCTCAAATGCAAAACACTTGAGGTTTTGTCATTGCATTCAAATTGCATTACCTACCCTGCTGCTACAGGAGGGTGTTTCCCATGCCTCAAGGTCCTCTATGTATCAGATTTTGATGATGATTCAATGCAGAAACTTTTTTCTCACTTCCCTGTGCTCGAAGAGTTGACTATTGATGCACACCTTAGTTTTTACAATGTTTTGAATATCAAAATCTCTGCACCTGAATTGAAGACGCTGAGAATATTGTTGGACTATCCCAGCAACAACTATAAGCGTCCCAACAACATTTTTATCAATGCCCCGAAGCTGGAGAACCTTGAAGTCACAGATGGTGTTTTAACAAATTACAATTTGGAGAGTGCAAAATGTCCAGAAAGAACTAGGATTGTATATAATGACAGATGTCTGATTGCTGATGATGTTTGCTTTCGAGATAAAGATGATGTATTTGAAGATGAAGCTCCGAGATTCACTAACCGCGCAGCTGCTTTACTTACAAAAGTTCACAATGTTAAATTTCTGTCTCTTGATGCTCGTCCTAAATGTTTGGAGGCTTGGTGTGTACCTGCTTTGGAAAACTTGGTCAAACTGGAGCTGGCTCTTCGGGACTGCTACAACTGGGGATTGCTAACAGAGTTGCTGAAGAGATCACCTAAGCTGGAATATCTTGGCATGGAACATAGAAACACCAGTTTTGATGAATGGGAGATCCCAGAGTGTAAATGGAATCCACCCGAGTTTGTGCCTATTTGTTTGTCATCAAGCCTTAAGACTATCTGCGTAAAGGGATTCAAAGGAGGGCAGCATGAGATGGAAGTTGCAAAGTACTTGTTAAAATACGGTGAAGTTTTGAATACCATGATTATTTCAACCAAGAATCGGTTCAATAGGCAGGATGGTCATCCATATAAAACGGAGGAAGAGCTATGCGAGGAATTTCATGTGTGTCAAAAGGGTTCAAAGATTTGCCAAGTTAACCTGATTTATGATGCAGGTTCATATTTCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

478

Amino Acids

55.6

Weight (kDa)

6.88

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 15 - 54 1.1e-07 F-box domain
LRR_At1g61320_AtMIF1 PF23622 83 - 254 3.2e-11 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 107 - 321 1.8e-12 FBD-associated F-box protein At5g56370, LRR repeats
FBD PF08387 387 - 431 3.4e-13 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1000, 1409
AccI GTMKAC 1 cut(s) 180
AccII CGCG 2 cut(s) 318, 906
AciI CCGC 1 cut(s) 904
AclWI GGATC 1 cut(s) 1129
AcsI RAATTY 3 cut(s) 215, 941, 1361
AcuI CTGAAG 2 cut(s) 280, 1088
AfaI GTAC 4 cut(s) 231, 301, 990, 1253
AfiI CCNNNNNNNGG 2 cut(s) 733, 1305
AflII CTTAAG 1 cut(s) 1193
AjuI GAANNNNNNNTTGG 2 cut(s) 1292, 1324
AleI CACNNNNGTG 1 cut(s) 285
AluBI AGCT 6 cut(s) 733, 887, 911, 1021, 1084, 1351
AluI AGCT 6 cut(s) 733, 887, 911, 1021, 1084, 1351
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 2 cut(s) 156, 954
AlwI GGATC 1 cut(s) 1129
Ama87I CYCGRG 1 cut(s) 1160
ApeKI GCWGC 5 cut(s) 338, 485, 908, 911, 1228
ApoI RAATTY 3 cut(s) 215, 941, 1361
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 1 cut(s) 1307
AspLEI GCGC 1 cut(s) 908
AspS9I GGNCC 2 cut(s) 111, 517
AsuHPI GGTGA 2 cut(s) 1068, 1280
AsuII TTCGAA 1 cut(s) 259
AvaI CYCGRG 1 cut(s) 1160
AvaII GGWCC 2 cut(s) 111, 517
BbsI GAAGAC 2 cut(s) 263, 662
Bbv12I GWGCWC 1 cut(s) 582
BbvI GCAGC 5 cut(s) 325, 472, 898, 920, 1240
BccI CCATC 4 cut(s) 126, 749, 1231, 1316
BcoDI GTCTC 2 cut(s) 156, 954
BfaI CTAG 2 cut(s) 126, 807
BfmI CTRYAG 1 cut(s) 489
BfrI CTTAAG 1 cut(s) 1193
BfuAI ACCTGC 2 cut(s) 1000, 1409
BisI GCNGC 5 cut(s) 339, 486, 909, 912, 1229
BlsI GCNGC 5 cut(s) 340, 487, 910, 913, 1230
BmcAI AGTACT 2 cut(s) 301, 1253
Bme18I GGWCC 2 cut(s) 111, 517
BmeT110I CYCGRG 1 cut(s) 1160
BmgT120I GGNCC 2 cut(s) 111, 517
BmrI ACTGGG 2 cut(s) 312, 1054
BmsI GCATC 4 cut(s) 49, 589, 946, 1405
BmuI ACTGGG 2 cut(s) 312, 1054
BoxI GACNNNNGTC 1 cut(s) 828
BpiI GAAGAC 2 cut(s) 263, 662
BpmI CTGGAG 2 cut(s) 755, 1037
Bpu10I CCTNAGC 1 cut(s) 1080
Bpu14I TTCGAA 1 cut(s) 259
BpuEI CTTGAG 2 cut(s) 464, 497
BsaBI GATNNNNATC 1 cut(s) 1326
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 241
BsaXI ACNNNNNCTCC 2 cut(s) 968, 998
Bsc4I CCNNNNNNNGG 2 cut(s) 733, 1305
Bse1I ACTGG 4 cut(s) 307, 1020, 1049, 1116
Bse3DI GCAATG 2 cut(s) 50, 455
Bse8I GATNNNNATC 1 cut(s) 1326
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 3 cut(s) 276, 1327, 1327
BseJI GATNNNNATC 1 cut(s) 1326
BseLI CCNNNNNNNGG 2 cut(s) 733, 1305
BseMI GCAATG 2 cut(s) 50, 455
BseMII CTCAG 1 cut(s) 653
BseNI ACTGG 4 cut(s) 307, 1020, 1049, 1116
BseXI GCAGC 5 cut(s) 325, 472, 898, 920, 1240
BseYI CCCAGC 1 cut(s) 683
BsgI GTGCAG 1 cut(s) 627
Bsh1236I CGCG 2 cut(s) 318, 906
BsiHKAI GWGCWC 1 cut(s) 582
BsiHKCI CYCGRG 1 cut(s) 1160
BslFI GGGAC 2 cut(s) 687, 1046
BslI CCNNNNNNNGG 2 cut(s) 733, 1305
BsmAI GTCTC 2 cut(s) 156, 954
BsmFI GGGAC 2 cut(s) 687, 1046
BsmI GAATGC 1 cut(s) 460
Bso31I GGTCTC 1 cut(s) 156
BsoBI CYCGRG 1 cut(s) 1160
Bsp119I TTCGAA 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 582
Bsp143I GATC 4 cut(s) 202, 367, 1073, 1134
BspACI CCGC 1 cut(s) 904
BspCNI CTCAG 1 cut(s) 654
BspFNI CGCG 2 cut(s) 318, 906
BspMI ACCTGC 2 cut(s) 1000, 1409
BspPI GGATC 1 cut(s) 1129
BspQI GCTCTTC 2 cut(s) 1032, 1341
BspT104I TTCGAA 1 cut(s) 259
BspTI CTTAAG 1 cut(s) 1193
BspTNI GGTCTC 1 cut(s) 156
BsrDI GCAATG 2 cut(s) 50, 455
BsrI ACTGG 4 cut(s) 307, 1020, 1049, 1116
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 4 cut(s) 202, 367, 1073, 1134
Bst4CI ACNGT 2 cut(s) 81, 1268
Bst6I CTCTTC 5 cut(s) 159, 579, 1032, 1064, 1341
BstAFI CTTAAG 1 cut(s) 1193
BstBI TTCGAA 1 cut(s) 259
BstC8I GCNNGC 1 cut(s) 1023
BstDEI CTNAG 4 cut(s) 419, 608, 662, 1080
BstDSI CCRYGG 1 cut(s) 241
BstF5I GGATG 3 cut(s) 276, 1327, 1327
BstFNI CGCG 2 cut(s) 318, 906
BstHHI GCGC 1 cut(s) 908
BstKTI GATC 4 cut(s) 205, 370, 1076, 1137
BstMAI GTCTC 2 cut(s) 156, 954
BstMBI GATC 4 cut(s) 202, 367, 1073, 1134
BstPAI GACNNNNGTC 1 cut(s) 828
BstSFI CTRYAG 1 cut(s) 489
BstUI CGCG 2 cut(s) 318, 906
BstV1I GCAGC 5 cut(s) 325, 472, 898, 920, 1240
BstV2I GAAGAC 2 cut(s) 263, 662
BstX2I RGATCY 1 cut(s) 1134
BstYI RGATCY 1 cut(s) 1134
BtgI CCRYGG 1 cut(s) 241
BtsCI GGATG 3 cut(s) 276, 1327, 1327
BveI ACCTGC 2 cut(s) 1000, 1409
Cac8I GCNNGC 1 cut(s) 1023
CfoI GCGC 1 cut(s) 908
Cfr13I GGNCC 2 cut(s) 111, 517
CseI GACGC 3 cut(s) 8, 667, 689
Csp6I GTAC 4 cut(s) 230, 300, 989, 1252
CviAII CATG 5 cut(s) 507, 1099, 1232, 1285, 1367
CviQI GTAC 4 cut(s) 230, 300, 989, 1252
DdeI CTNAG 4 cut(s) 419, 608, 662, 1080
DpnI GATC 4 cut(s) 204, 369, 1075, 1136
DpnII GATC 4 cut(s) 202, 367, 1073, 1134
Eam1104I CTCTTC 5 cut(s) 159, 579, 1032, 1064, 1341
EarI CTCTTC 5 cut(s) 159, 579, 1032, 1064, 1341
Eco31I GGTCTC 1 cut(s) 156
Eco47I GGWCC 2 cut(s) 111, 517
Eco57I CTGAAG 2 cut(s) 280, 1088
Eco88I CYCGRG 1 cut(s) 1160
EcoO109I RGGNCCY 1 cut(s) 517
FaeI CATG 5 cut(s) 510, 1102, 1235, 1288, 1370
FaqI GGGAC 2 cut(s) 687, 1046
FatI CATG 5 cut(s) 506, 1098, 1231, 1284, 1366
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 5 cut(s) 339, 486, 909, 912, 1229
FokI GGATG 3 cut(s) 263, 1314, 1334
Fsp4HI GCNGC 5 cut(s) 339, 486, 909, 912, 1229
FspBI CTAG 2 cut(s) 126, 807
GlaI GCGC 1 cut(s) 907
GluI GCNGC 5 cut(s) 339, 486, 909, 912, 1229
GsaI CCCAGC 1 cut(s) 687
GsuI CTGGAG 2 cut(s) 755, 1037
HgaI GACGC 3 cut(s) 8, 667, 689
HhaI GCGC 1 cut(s) 908
Hin1II CATG 5 cut(s) 510, 1102, 1235, 1288, 1370
Hin6I GCGC 1 cut(s) 906
HinP1I GCGC 1 cut(s) 906
HincII GTYRAC 3 cut(s) 23, 591, 1402
HindII GTYRAC 3 cut(s) 23, 591, 1402
HinfI GANTC 8 cut(s) 5, 11, 331, 545, 894, 1153, 1215, 1303
HpaI GTTAAC 1 cut(s) 1402
HphI GGTGA 2 cut(s) 1068, 1280
Hpy166II GTNNAC 8 cut(s) 23, 111, 181, 230, 591, 931, 989, 1402
Hpy188I TCNGA 6 cut(s) 10, 253, 324, 532, 834, 891
Hpy188III TCNNGA 4 cut(s) 797, 857, 953, 1031
Hpy8I GTNNAC 8 cut(s) 23, 111, 181, 230, 591, 931, 989, 1402
HpyAV CCTTC 4 cut(s) 98, 128, 247, 434
HpyCH4III ACNGT 2 cut(s) 81, 1268
HpyCH4IV ACGT 1 cut(s) 177
HpyF3I CTNAG 4 cut(s) 419, 608, 662, 1080
HpySE526I ACGT 1 cut(s) 177
Hsp92II CATG 5 cut(s) 510, 1102, 1235, 1288, 1370
HspAI GCGC 1 cut(s) 906
KspAI GTTAAC 1 cut(s) 1402
Kzo9I GATC 4 cut(s) 202, 367, 1073, 1134
LguI GCTCTTC 2 cut(s) 1032, 1341
LmnI GCTCC 2 cut(s) 892, 1018
Lsp1109I GCAGC 5 cut(s) 325, 472, 898, 920, 1240
LweI GCATC 4 cut(s) 49, 589, 946, 1405
MaeI CTAG 2 cut(s) 126, 807
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 1 cut(s) 748
MalI GATC 4 cut(s) 204, 369, 1075, 1136
MboI GATC 4 cut(s) 202, 367, 1073, 1134
MboII GAAGA 8 cut(s) 146, 263, 596, 667, 890, 1019, 1081, 1358
MflI RGATCY 1 cut(s) 1134
MhlI GDGCHC 1 cut(s) 582
MluCI AATT 9 cut(s) 42, 160, 215, 466, 650, 769, 775, 941, 1361
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 654
MroXI GAANNNNTTC 1 cut(s) 1307
MseI TTAA 5 cut(s) 764, 939, 1194, 1259, 1401
MslI CAYNNNNRTG 1 cut(s) 285
MspA1I CMGCKG 1 cut(s) 911
MspCI CTTAAG 1 cut(s) 1193
Mva1269I GAATGC 1 cut(s) 460
MvnI CGCG 2 cut(s) 318, 906
NdeII GATC 4 cut(s) 202, 367, 1073, 1134
NlaIII CATG 5 cut(s) 510, 1102, 1235, 1288, 1370
NmuCI GTSAC 1 cut(s) 748
NspV TTCGAA 1 cut(s) 259
OliI CACNNNNGTG 1 cut(s) 285
PciSI GCTCTTC 2 cut(s) 1032, 1341
PctI GAATGC 1 cut(s) 460
PdmI GAANNNNTTC 1 cut(s) 1307
PfeI GAWTC 7 cut(s) 5, 331, 545, 894, 1153, 1215, 1303
PkrI GCNGC 5 cut(s) 340, 487, 910, 913, 1230
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
PpuMI RGGWCCY 1 cut(s) 517
PshAI GACNNNNGTC 1 cut(s) 828
Psp5II RGGWCCY 1 cut(s) 517
PspFI CCCAGC 1 cut(s) 683
PspPI GGNCC 2 cut(s) 111, 517
PspPPI RGGWCCY 1 cut(s) 517
PsuI RGATCY 1 cut(s) 1134
PvuII CAGCTG 1 cut(s) 911
RsaI GTAC 4 cut(s) 231, 301, 990, 1253
RsaNI GTAC 4 cut(s) 230, 300, 989, 1252
RseI CAYNNNNRTG 1 cut(s) 285
SapI GCTCTTC 2 cut(s) 1032, 1341
SaqAI TTAA 5 cut(s) 764, 939, 1194, 1259, 1401
SatI GCNGC 5 cut(s) 339, 486, 909, 912, 1229
Sau3AI GATC 4 cut(s) 202, 367, 1073, 1134
Sau96I GGNCC 2 cut(s) 111, 517
ScaI AGTACT 2 cut(s) 301, 1253
SchI GAGTC 1 cut(s) 20
SduI GDGCHC 1 cut(s) 582
SfaNI GCATC 4 cut(s) 49, 589, 946, 1405
SfcI CTRYAG 1 cut(s) 489
SfuI TTCGAA 1 cut(s) 259
SinI GGWCC 2 cut(s) 111, 517
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 3 cut(s) 443, 512, 1193
SmoI CTYRAG 3 cut(s) 443, 512, 1193
Sse9I AATT 9 cut(s) 42, 160, 215, 466, 650, 769, 775, 941, 1361
SsiI CCGC 1 cut(s) 904
SspI AATATT 2 cut(s) 355, 669
SspMI CTAG 2 cut(s) 126, 807
TaaI ACNGT 2 cut(s) 81, 1268
TaiI ACGT 1 cut(s) 180
TaqI TCGA 3 cut(s) 259, 582, 856
TasI AATT 9 cut(s) 42, 160, 215, 466, 650, 769, 775, 941, 1361
TatI WGTACW 3 cut(s) 229, 299, 1251
TfiI GAWTC 7 cut(s) 5, 331, 545, 894, 1153, 1215, 1303
Tru1I TTAA 5 cut(s) 764, 939, 1194, 1259, 1401
Tru9I TTAA 5 cut(s) 764, 939, 1194, 1259, 1401
TseFI GTSAC 1 cut(s) 748
TseI GCWGC 5 cut(s) 338, 485, 908, 911, 1228
Tsp45I GTSAC 1 cut(s) 748
TspDTI ATGAA 5 cut(s) 227, 897, 1140, 1355, 1413
TspGWI ACGGA 2 cut(s) 230, 1355
Vha464I CTTAAG 1 cut(s) 1193
VpaK11BI GGWCC 2 cut(s) 111, 517
XapI RAATTY 3 cut(s) 215, 941, 1361
XcmI CCANNNNNNNNNTGG 1 cut(s) 1146
XmiI GTMKAC 1 cut(s) 180
XmnI GAANNNNTTC 1 cut(s) 1307
XspI CTAG 2 cut(s) 126, 807
ZrmI AGTACT 2 cut(s) 301, 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.