Rh5DG309200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010590
Physical Location & Seq
Reverse (-)
39506554 .. 39507159
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG309200.1

Sequence Viewer

Length: 606 bp
ATGTTTGATGGCAATGATTGGTGTGAACTGGCACCACCTCCAATCAGAAAATATTTCTGTTATCCCTTTACACACTCCGACCCGGCTTGTCAGTTCGATATATACTATGTAGTTGCAGGCACAAATATATTTGTTTCTGATGGTGGTTTAAGCTTCCGCTTTGACACAGCTGACCCCAACCAAGGCTGGATAAAACTAATGTGGGTCGATCAATTCTGTTTTAAGGGCACATGTTTGGTTGTGAATGATGGTAAGCAATTTGTGCTTTTCACATTCCAACCCTCCGGTGGTCAGCACATATCAGTGCATCTGATTTCATATGACTTCAATAGCATCACACAAGACTACAGTCAAGTGCAGCTACCTTCGTACTTAATGGAATGCAAGGGTGATCGTCTTGGTGATTTTTCATTACCCAATTACCAGCTGGTACACCTAGGAGATAAGGCCGTCTGCCTTCTTATGTCTGAACTTCGCCCCAGTGGAGTGGATGAGAGCGAGAAGATGATTAGGTCTGTTGAATTTGCCACATTCACATTCAAAGTCAAAGACAATGCTAAGTCCTTGAAAATCAGATTTCTACCTCATACTCCAAAGCTCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

22.94

Weight (kDa)

5.68

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 6 - 198 9.2e-07 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 31
AciI CCGC 1 cut(s) 157
AcsI RAATTY 1 cut(s) 521
AfaI GTAC 2 cut(s) 371, 432
AfiI CCNNNNNNNGG 2 cut(s) 182, 287
AflIII ACRYGT 1 cut(s) 230
AgsI TTSAA 4 cut(s) 328, 521, 541, 568
AluBI AGCT 5 cut(s) 153, 170, 361, 427, 598
AluI AGCT 5 cut(s) 153, 170, 361, 427, 598
AoxI GGCC 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 358
ApoI RAATTY 1 cut(s) 521
AspA2I CCTAGG 1 cut(s) 436
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 2 cut(s) 401, 413
AvrII CCTAGG 1 cut(s) 436
BaeGI GKGCMC 1 cut(s) 230
BanI GGYRCC 1 cut(s) 31
BbvI GCAGC 1 cut(s) 370
BccI CCATC 3 cut(s) 2, 134, 242
BceAI ACGGC 1 cut(s) 434
BcnI CCSGG 1 cut(s) 83
BfaI CTAG 1 cut(s) 437
BfmI CTRYAG 1 cut(s) 346
BisI GCNGC 1 cut(s) 359
BlnI CCTAGG 1 cut(s) 436
BlsI GCNGC 1 cut(s) 360
Bme1390I CCNGG 1 cut(s) 83
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 1 cut(s) 83
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 2 cut(s) 316, 342
BmuI ACTGGG 1 cut(s) 474
BoxI GACNNNNGTC 1 cut(s) 348
BpuMI CCSGG 1 cut(s) 83
BsaJI CCNNGG 2 cut(s) 181, 436
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 287
Bse1I ACTGG 2 cut(s) 33, 480
Bse3DI GCAATG 1 cut(s) 19
BseDI CCNNGG 2 cut(s) 181, 436
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 2 cut(s) 182, 287
BseMI GCAATG 1 cut(s) 19
BseNI ACTGG 2 cut(s) 33, 480
BseSI GKGCMC 1 cut(s) 230
BseXI GCAGC 1 cut(s) 370
BsgI GTGCAG 1 cut(s) 377
BshFI GGCC 1 cut(s) 449
BshNI GGYRCC 1 cut(s) 31
BsiSI CCGG 2 cut(s) 83, 285
BslI CCNNNNNNNGG 2 cut(s) 182, 287
BsmI GAATGC 1 cut(s) 386
BsnI GGCC 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 230
Bsp143I GATC 2 cut(s) 208, 391
BspACI CCGC 1 cut(s) 157
BspANI GGCC 1 cut(s) 449
BspLI GGNNCC 1 cut(s) 33
BspT107I GGYRCC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 19
BsrI ACTGG 2 cut(s) 33, 480
BssECI CCNNGG 2 cut(s) 181, 436
BssMI GATC 2 cut(s) 208, 391
BssT1I CCWWGG 2 cut(s) 181, 436
Bst4CI ACNGT 1 cut(s) 350
BstAPI GCANNNNNTGC 1 cut(s) 262
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 1 cut(s) 558
BstF5I GGATG 1 cut(s) 496
BstKTI GATC 2 cut(s) 211, 394
BstMBI GATC 2 cut(s) 208, 391
BstMWI GCNNNNNNNGC 1 cut(s) 262
BstNSI RCATGY 1 cut(s) 234
BstPAI GACNNNNGTC 1 cut(s) 348
BstSCI CCNGG 1 cut(s) 81
BstSFI CTRYAG 1 cut(s) 346
BstSLI GKGCMC 1 cut(s) 230
BstV1I GCAGC 1 cut(s) 370
BstXI CCANNNNNNTGG 1 cut(s) 487
BsuRI GGCC 1 cut(s) 449
BtsCI GGATG 1 cut(s) 496
BtsIMutI CAGTG 2 cut(s) 309, 487
Cac8I GCNNGC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 370, 431
CviAII CATG 1 cut(s) 231
CviJI RGCY 8 cut(s) 86, 153, 170, 186, 361, 427, 449, 598
CviKI_1 RGCY 8 cut(s) 86, 153, 170, 186, 361, 427, 449, 598
CviQI GTAC 2 cut(s) 370, 431
DdeI CTNAG 1 cut(s) 558
DpnI GATC 2 cut(s) 210, 393
DpnII GATC 2 cut(s) 208, 391
Eco130I CCWWGG 2 cut(s) 181, 436
EcoT14I CCWWGG 2 cut(s) 181, 436
ErhI CCWWGG 2 cut(s) 181, 436
FaeI CATG 1 cut(s) 234
FatI CATG 1 cut(s) 230
FauNDI CATATG 1 cut(s) 319
Fnu4HI GCNGC 1 cut(s) 359
FokI GGATG 1 cut(s) 503
Fsp4HI GCNGC 1 cut(s) 359
FspBI CTAG 1 cut(s) 437
GluI GCNGC 1 cut(s) 359
HaeIII GGCC 1 cut(s) 449
HapII CCGG 2 cut(s) 83, 285
Hin1II CATG 1 cut(s) 234
HindIII AAGCTT 1 cut(s) 151
HpaII CCGG 2 cut(s) 83, 285
HphI GGTGA 2 cut(s) 401, 413
Hpy166II GTNNAC 2 cut(s) 26, 433
Hpy188I TCNGA 6 cut(s) 47, 79, 139, 312, 469, 575
Hpy8I GTNNAC 2 cut(s) 26, 433
HpyAV CCTTC 2 cut(s) 375, 467
HpyCH4III ACNGT 1 cut(s) 350
HpyCH4V TGCA 4 cut(s) 116, 307, 358, 384
HpyF10VI GCNNNNNNNGC 1 cut(s) 262
HpyF3I CTNAG 1 cut(s) 558
Hsp92II CATG 1 cut(s) 234
Kzo9I GATC 2 cut(s) 208, 391
LpnPI CCDG 8 cut(s) 14, 96, 102, 172, 298, 413, 437, 493
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 2 cut(s) 316, 342
MaeI CTAG 1 cut(s) 437
MalI GATC 2 cut(s) 210, 393
MboI GATC 2 cut(s) 208, 391
MboII GAAGA 1 cut(s) 514
MhlI GDGCHC 1 cut(s) 230
MluCI AATT 4 cut(s) 212, 257, 418, 521
MmeI TCCRAC 2 cut(s) 102, 301
MnlI CCTC 3 cut(s) 48, 292, 594
MseI TTAA 3 cut(s) 149, 222, 374
MslI CAYNNNNRTG 1 cut(s) 302
MspA1I CMGCKG 2 cut(s) 170, 427
MspI CCGG 2 cut(s) 83, 285
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 1 cut(s) 386
MwoI GCNNNNNNNGC 1 cut(s) 262
NciI CCSGG 1 cut(s) 83
NdeI CATATG 1 cut(s) 319
NdeII GATC 2 cut(s) 208, 391
NlaIII CATG 1 cut(s) 234
NlaIV GGNNCC 1 cut(s) 33
NspI RCATGY 1 cut(s) 234
PciI ACATGT 1 cut(s) 230
PctI GAATGC 1 cut(s) 386
PkrI GCNGC 1 cut(s) 360
PscI ACATGT 1 cut(s) 230
PshAI GACNNNNGTC 1 cut(s) 348
PspN4I GGNNCC 1 cut(s) 33
PvuII CAGCTG 2 cut(s) 170, 427
RsaI GTAC 2 cut(s) 371, 432
RsaNI GTAC 2 cut(s) 370, 431
RseI CAYNNNNRTG 1 cut(s) 302
SaqAI TTAA 3 cut(s) 149, 222, 374
SatI GCNGC 1 cut(s) 359
Sau3AI GATC 2 cut(s) 208, 391
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 1 cut(s) 230
SfaNI GCATC 2 cut(s) 316, 342
SfcI CTRYAG 1 cut(s) 346
SmiMI CAYNNNNRTG 1 cut(s) 302
Sse9I AATT 4 cut(s) 212, 257, 418, 521
SsiI CCGC 1 cut(s) 157
SspI AATATT 1 cut(s) 53
SspMI CTAG 1 cut(s) 437
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 2 cut(s) 181, 436
TaaI ACNGT 1 cut(s) 350
TaqI TCGA 2 cut(s) 96, 207
TasI AATT 4 cut(s) 212, 257, 418, 521
Tru1I TTAA 3 cut(s) 149, 222, 374
Tru9I TTAA 3 cut(s) 149, 222, 374
TscAI CASTG 2 cut(s) 309, 487
TseI GCWGC 1 cut(s) 358
TspDTI ATGAA 2 cut(s) 306, 399
TspRI CASTG 2 cut(s) 309, 487
XapI RAATTY 1 cut(s) 521
XceI RCATGY 1 cut(s) 234
XcmI CCANNNNNNNNNTGG 2 cut(s) 284, 424
XmaJI CCTAGG 1 cut(s) 436
XspI CTAG 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.